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MXPA05010478A - Procedimiento para la tincion de esperma. - Google Patents

Procedimiento para la tincion de esperma.

Info

Publication number
MXPA05010478A
MXPA05010478A MXPA05010478A MXPA05010478A MXPA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A
Authority
MX
Mexico
Prior art keywords
sperm
cells
sperm cells
staining
involve
Prior art date
Application number
MXPA05010478A
Other languages
English (en)
Inventor
Jeannet A Glaenzer
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of MXPA05010478A publication Critical patent/MXPA05010478A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Reproductive Health (AREA)
  • Endocrinology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Las celulas de esperma se tinen de conformidad con procedimientos que implican la combinacion de celulas de esperma con un colorante selectivo de ADN fluorescente a una temperatura elevada en exceso de aproximadamente 40°C; los metodos permiten un tiempo de tincion disminuido; las celulas pueden de aqui en adelante clasificarse eficazmente de conformidad con metodos de separacion comunes, incluyendo citometria de flujo.
MXPA05010478A 2003-03-28 2004-03-29 Procedimiento para la tincion de esperma. MXPA05010478A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45873103P 2003-03-28 2003-03-28
PCT/US2004/009903 WO2004087177A1 (en) 2003-03-28 2004-03-29 Process for the staining of sperm

Publications (1)

Publication Number Publication Date
MXPA05010478A true MXPA05010478A (es) 2005-12-15

Family

ID=33131822

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05010478A MXPA05010478A (es) 2003-03-28 2004-03-29 Procedimiento para la tincion de esperma.

Country Status (14)

Country Link
US (1) US7335507B2 (es)
EP (1) EP1608382B1 (es)
JP (1) JP2006525019A (es)
CN (1) CN100379429C (es)
AT (1) ATE369867T1 (es)
AU (1) AU2004226343B2 (es)
BR (1) BRPI0408852A (es)
CA (1) CA2519989A1 (es)
DE (1) DE602004008241T2 (es)
MX (1) MXPA05010478A (es)
NZ (1) NZ542688A (es)
PL (1) PL1608382T3 (es)
WO (1) WO2004087177A1 (es)
ZA (1) ZA200507823B (es)

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DE69830598T2 (de) 1997-01-31 2006-05-18 The Horticulture And Food Research Institute Of New Zealand Limited Optische vorrichtung und methode
US6071689A (en) * 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6149867A (en) * 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
EP1100534B1 (en) 1998-07-30 2008-01-16 XY, Inc. Equine system for non-surgical artificial insemination
US7208265B1 (en) * 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
DK2258172T3 (en) * 2000-05-09 2017-08-07 Xy Llc Flow cytometer for differentiation of X-chromosome-bearing and Y-chromosome-bearing populations of spermatozoa
UY26761A1 (es) * 2000-06-12 2001-07-31 Univ Colorado State Res Found Integración de destete precoz y uso de semen de sexo seleccionado en un sistema de vaquillona- ternero único para incrementar el valor de vaquillonas de no reemplazo.
US20040031071A1 (en) * 2000-10-05 2004-02-12 Xy, Inc. System of hysteroscopic insemination of mares
BRPI0115792B1 (pt) 2000-11-29 2020-05-05 Colorado State Univ sistema para separação de espermatozóides congelados/descongelados em populações portadoras de cromossomo-x e portadoras de cromossomo-y
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
DK2283724T3 (en) 2002-08-01 2016-04-04 Xy Llc Heterospermic insemination to assess sperm function
MXPA05001654A (es) 2002-08-15 2005-10-18 Xy Inc Citometro de flujo de alta resolucion.
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
PL1608382T3 (pl) 2003-03-28 2008-03-31 Inguran Llc Sposób barwienia spermy
EP2306174B1 (en) 2003-03-28 2016-05-11 Inguran, LLC Flow cytometry nozzle for orienting particles and corresponding method
CA2566749C (en) * 2003-05-15 2017-02-21 Xy, Inc. Efficient haploid cell sorting for flow cytometer systems
EP1730523B1 (en) * 2004-03-29 2010-01-13 Inguran, LLC Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellularly in a staining or sorting process of spermatozoa
DK2801363T3 (en) 2004-03-29 2018-05-28 Inguran Llc PROCEDURE FOR STORING SORTED SPERMATOZOES
EP1769067A2 (en) 2004-07-22 2007-04-04 Monsanto Technology LLC Process for enriching a population of sperm cells
US7355696B2 (en) 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
KR100949923B1 (ko) * 2008-04-21 2010-03-30 중앙대학교 산학협력단 번식능력이 불량한 수퇘지 선별법
US20110236923A1 (en) * 2010-03-23 2011-09-29 Genetics & Ivf Institute Method for staining and sorting of a small volume of sperm
CA2794934A1 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
CN101793641B (zh) * 2010-04-14 2011-09-28 内蒙古蒙牛繁育生物技术股份有限公司 一种用于奶牛精子分离的荧光染色方法
CN102095675B (zh) * 2010-11-23 2012-05-23 内蒙古赛科星繁育生物技术股份有限公司 混合奶牛性控冻精产品鉴定和检测方法
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
EP2713713A4 (en) 2011-06-01 2014-12-10 Inguran Llc COMPOSITIONS AND METHODS FOR IMPROVING THE QUALITY OF PROCESSED SPERM
DK2761275T3 (en) 2011-09-30 2017-09-25 Inguran Llc METHODS FOR COLORING AND SORTING SPERMA
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
BR112015007480B1 (pt) 2012-10-05 2020-04-22 Inguran Llc métodos de separação de esperma com base no sexo de eficácia elevada.
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
CN103335934A (zh) * 2013-06-28 2013-10-02 浙江星博生物科技有限公司 一种基于流式细胞术的精子活率检测试剂
CN103344543B (zh) * 2013-06-28 2016-07-06 浙江星博生物科技股份有限公司 一种基于流式细胞术的精子线粒体膜电位检测试剂
CN103555662B (zh) * 2013-10-31 2015-09-16 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
WO2019199852A1 (en) * 2018-04-09 2019-10-17 Inguran, Llc Improved sperm nuclei and methods of their manufacture and use
US10470798B1 (en) 2018-11-30 2019-11-12 Ohana Biosciences, Inc. Methods for promoting fertilization

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US3005756A (en) 1958-11-14 1961-10-24 Noland L Van Demark Diluter containing carbon dioxide for preserving semen
US4474875A (en) 1969-04-10 1984-10-02 Wallace Shrimpton Method and means for controlling the sex of mammalian offspring and product therefor
US3791384A (en) 1971-07-15 1974-02-12 Schaumann H Artificial insemination of sows
US4267268A (en) 1979-03-12 1981-05-12 Nelson Jr Robert A Spermatozoa extenders
US4362246A (en) 1980-07-14 1982-12-07 Adair Edwin Lloyd Method of treating collected mammal semen and separating sperm into X Y components
GB2145112B (en) 1983-04-27 1987-02-18 Milk Marketing Board Sorting living spermatozoa
ATE142788T1 (de) * 1989-05-10 1996-09-15 Us Agriculture Verfahren zur vorwahl des geschlechts der nachkommenschaft
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
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US6040139A (en) 1995-09-19 2000-03-21 Bova; G. Steven Laser cell purification system
ES2186732T3 (es) 1995-11-09 2003-05-16 Medical Res Council Uso de medio cyb para el transporte y almacenamiento de esperma.
US5985216A (en) 1997-07-24 1999-11-16 The United States Of America, As Represented By The Secretary Of Agriculture Flow cytometry nozzle for high efficiency cell sorting
CA2304197C (en) 1997-09-22 2009-02-03 University Of Guelph Reduction of sperm sensitivity to chilling
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6642018B1 (en) 1998-03-27 2003-11-04 Oncosis Llc Method for inducing a response in one or more targeted cells
US6309815B1 (en) 1998-08-07 2001-10-30 University Of Kansas Medical Center Composition and method for preparation, storage and activation of large populations of immotile sperm
WO2000054026A1 (en) 1999-03-05 2000-09-14 Hatting-Ks Determination of sperm concentration and viability for artificial insemination
FR2793495B1 (fr) 1999-05-14 2001-08-10 Imv Technologies Dilueur pour la cryoconservation de spermatozoides de bovins
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US6263745B1 (en) 1999-12-03 2001-07-24 Xy, Inc. Flow cytometer nozzle and flow cytometer sample handling methods
IT1317724B1 (it) * 2000-01-14 2003-07-15 Istituto Sperimentale Italiano Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico.
DK2258172T3 (en) 2000-05-09 2017-08-07 Xy Llc Flow cytometer for differentiation of X-chromosome-bearing and Y-chromosome-bearing populations of spermatozoa
US7193706B2 (en) 2000-08-02 2007-03-20 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Computer interfaced scanning fluorescence lifetime microscope applied to directed evolution
US20050064383A1 (en) * 2000-11-22 2005-03-24 Bashkin James K. Methods and apparatus for producing gender enriched sperm
BRPI0115792B1 (pt) 2000-11-29 2020-05-05 Colorado State Univ sistema para separação de espermatozóides congelados/descongelados em populações portadoras de cromossomo-x e portadoras de cromossomo-y
AU2002254173A1 (en) * 2001-03-12 2002-10-08 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The Unive Oxidation-reduction sensitive green fluorescent protein variants
EP2306174B1 (en) 2003-03-28 2016-05-11 Inguran, LLC Flow cytometry nozzle for orienting particles and corresponding method
PL1608382T3 (pl) 2003-03-28 2008-03-31 Inguran Llc Sposób barwienia spermy

Also Published As

Publication number Publication date
CA2519989A1 (en) 2004-10-14
CN100379429C (zh) 2008-04-09
NZ542688A (en) 2008-06-30
AU2004226343A1 (en) 2004-10-14
ATE369867T1 (de) 2007-09-15
EP1608382B1 (en) 2007-08-15
CN1795004A (zh) 2006-06-28
PL1608382T3 (pl) 2008-03-31
WO2004087177A1 (en) 2004-10-14
BRPI0408852A (pt) 2006-04-04
JP2006525019A (ja) 2006-11-09
AU2004226343B2 (en) 2008-10-09
DE602004008241T2 (de) 2008-05-08
ZA200507823B (en) 2008-03-26
US20050003472A1 (en) 2005-01-06
EP1608382A1 (en) 2005-12-28
US7335507B2 (en) 2008-02-26
DE602004008241D1 (de) 2007-09-27

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