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MX9401112A - Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos. - Google Patents

Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos.

Info

Publication number
MX9401112A
MX9401112A MX9401112A MX9401112A MX9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A
Authority
MX
Mexico
Prior art keywords
conjugates
methods
present
preparation
photoprotein
Prior art date
Application number
MX9401112A
Other languages
English (en)
Inventor
Nancy L Stults
Original Assignee
Sealite Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sealite Sciences Inc filed Critical Sealite Sciences Inc
Publication of MX9401112A publication Critical patent/MX9401112A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención abarca un método para la síntesis de conjugados de fotoproteínas que retienen toda o una porción considerable de la actividad luminiscente de las fotoproteínas no derivatizadas. De conformidad con la presente invención, las fotoproteínas pueden conjugarse con una variedad de enlaces o uniones que incluyen estreptavidina/avidina, glicoproteínas, lectinas, hormonas, antígenos, fármacos, anticuerpos y fragmentos de unión de antígeno, de los mismos, o cualquier otro reactivo selectivamente ligable, por medios de reticulación química. La presente invención también abarca conjugados producidos por este método, y los métodos de uso de esos conjugados.
MX9401112A 1993-02-12 1994-02-11 Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos. MX9401112A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1711693A 1993-02-12 1993-02-12

Publications (1)

Publication Number Publication Date
MX9401112A true MX9401112A (es) 1994-08-31

Family

ID=21780817

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9401112A MX9401112A (es) 1993-02-12 1994-02-11 Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos.

Country Status (9)

Country Link
US (1) US5486455A (es)
EP (1) EP0683822B1 (es)
JP (1) JPH08506897A (es)
AT (1) ATE217086T1 (es)
AU (1) AU6171894A (es)
DE (1) DE69430523T2 (es)
IL (1) IL108607A (es)
MX (1) MX9401112A (es)
WO (1) WO1994018342A1 (es)

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DE4438660A1 (de) * 1994-10-28 1996-05-02 Boehringer Mannheim Gmbh Biolumineszenzmarkierte Hapten-Konjugate für den Einsatz in kompetitiven Immunoassays
EP0736770A3 (fr) * 1995-04-05 1997-05-02 Anda Biolog Sa Conjugué immunoréactif, procédé d'obtention de ce conjugué, anticorps dirigés contre ledit conjugué, composition pharmaceutique et dispositif de diagnostic les comprenant
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
US5876995A (en) * 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US5942407A (en) * 1996-06-25 1999-08-24 Immunomatrix, Inc. Light-emitting immunoassay
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
EP1015872B1 (en) 1996-12-12 2005-03-02 Prolume, Ltd. Apparatus and method for detecting and identifying infectious agents
DE19652576A1 (de) * 1996-12-17 1998-06-25 Boehringer Mannheim Gmbh Niedermolekulare Photoprotein-Konjugate
US5876935A (en) * 1997-01-08 1999-03-02 Dade Behring Inc. Luminescent specific binding assay
WO1998036081A2 (en) 1997-02-13 1998-08-20 Memorial Sloan-Kettering Cancer Center Hybrid molecules for optically detecting changes in cellular microenvironments
CA2283094A1 (en) * 1997-02-18 1998-08-20 Oncor, Inc. Detection of targets with green fluorescent protein and fluorescent variants thereof
DE19719465A1 (de) * 1997-05-07 1998-11-12 Boehringer Mannheim Gmbh Konservierung von Aequorin-Reagenzien
EP1925320A3 (en) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics
EP1064360B1 (en) 1998-03-27 2008-03-05 Prolume, Ltd. Luciferases, gfp fluorescent proteins, their nucleic acids and the use thereof in diagnostics
EP1194781B1 (en) * 1999-06-18 2007-03-07 Cardiogenics Inc. Method for conducting chemiluminescent binding assay
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US7659078B1 (en) * 2002-07-17 2010-02-09 University Of Kentucky Research Foundation Method and kit for determination of prostacyclin in plasma
US20080280310A1 (en) * 2007-05-09 2008-11-13 Louis Panagopoulos Testing for Blood Group Immunological Reaction Without the Use of Anti-Human Globulin

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US4604364A (en) * 1974-01-04 1986-08-05 Kosak Kenneth M Bioluminescent tracer composition and method of use in immunoassays
GB1578275A (en) * 1977-06-14 1980-11-05 Maier C Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids
US4396579A (en) * 1981-08-06 1983-08-02 Miles Laboratories, Inc. Luminescence detection device
US4608246A (en) * 1983-03-10 1986-08-26 Immucor, Inc. Testing for a blood group immunological reaction
ES8607563A1 (es) * 1983-10-13 1986-06-01 Univ Georgia Res Found Un metodo para detectar una interaccion ligante
US4665022A (en) * 1984-02-17 1987-05-12 The Regents Of The University Of California Bioluminescent assay reagent and method
US4927916A (en) * 1984-04-23 1990-05-22 The General Hospital Corporation Method of producing fibrin-specific monoclonal antibodies lacking fibrinogen-cross-reactivity using fibrin-specific peptides
US4764368A (en) * 1984-08-29 1988-08-16 Dana-Farber Cancer Institute, Inc. Acid-cleavable compound
US4657853A (en) * 1984-09-14 1987-04-14 E. I. Du Pont De Nemours And Company Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody
US4935339A (en) * 1985-05-07 1990-06-19 Nichols Institute Diagnostics Delayed solid phase immunologic assay
DE3537877A1 (de) * 1985-10-24 1987-04-30 Geiger Reinhard Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate
GB8603537D0 (en) * 1986-02-13 1986-03-19 Parker D Conjugate compound
US4916070A (en) * 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
DE3714147A1 (de) * 1987-04-28 1988-11-17 Boehringer Mannheim Gmbh Immunchemisches verfahren und reagenz zur bestimmung eines polyvalenten antigens in einer fluessigen probe
JPS6447379A (en) * 1987-08-19 1989-02-21 Chisso Corp Preparation of calcium-dependent oxygenation enzyme
US4981979A (en) * 1987-09-10 1991-01-01 Neorx Corporation Immunoconjugates joined by thioether bonds having reduced toxicity and improved selectivity
US5094939A (en) * 1988-07-19 1992-03-10 Fujirebio, Inc. Chemiluminescence assays using stabilized dioxetane derivatives
US4925648A (en) * 1988-07-29 1990-05-15 Immunomedics, Inc. Detection and treatment of infectious and inflammatory lesions
US5112615A (en) * 1988-08-03 1992-05-12 New England Deaconess Hospital Corporation Soluble hirudin conjugates
US5019393A (en) * 1988-08-03 1991-05-28 New England Deaconess Hospital Corporation Biocompatible substance with thromboresistance
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JPH02253162A (ja) * 1989-03-27 1990-10-11 Chisso Corp 特異的結合能を有する物質と結合したエクオリンを用いる検出法
JPH04502469A (ja) * 1988-12-22 1992-05-07 ゾマ　コーポレイション ヒンダードカップリング剤および方法
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US5116944A (en) * 1989-12-29 1992-05-26 Neorx Corporation Conjugates having improved characteristics for in vivo administration

Also Published As

Publication number Publication date
WO1994018342A1 (en) 1994-08-18
US5486455A (en) 1996-01-23
EP0683822A1 (en) 1995-11-29
IL108607A0 (en) 1994-05-30
ATE217086T1 (de) 2002-05-15
EP0683822B1 (en) 2002-05-02
JPH08506897A (ja) 1996-07-23
DE69430523T2 (de) 2002-11-28
DE69430523D1 (de) 2002-06-06
IL108607A (en) 1998-12-06
AU6171894A (en) 1994-08-29
EP0683822A4 (en) 1997-11-05

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