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MX2023004594A - Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas. - Google Patents

Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas.

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Publication number
MX2023004594A
MX2023004594A MX2023004594A MX2023004594A MX2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A MX 2023004594 A MX2023004594 A MX 2023004594A
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Mexico
Prior art keywords
cells
corneal endothelial
human corneal
time
sup
Prior art date
Application number
MX2023004594A
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English (en)
Inventor
Shigeru Kinoshita
Munetoyo TODA
Chie Sotozono
Morio Ueno
Original Assignee
Kyoto Prefectural Public Univ Corp
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Publication date
Application filed by Kyoto Prefectural Public Univ Corp filed Critical Kyoto Prefectural Public Univ Corp
Publication of MX2023004594A publication Critical patent/MX2023004594A/es

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    • C12N1/04—Preserving or maintaining viable microorganisms
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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Abstract

Las células endoteliales de la córnea humana y/o las células precursoras endoteliales de la córnea humana se conservan con una alta tasa de supervivencia de estas células manteniéndose, y se suprime la tasa de aparición de células contaminadas en el cultivo posterior a la conservación. Un método de almacenamiento de células endoteliales de la córnea humana y/o células precursoras endoteliales de la córnea humana se caracteriza porque las células endoteliales de la córnea humana y/o las células precursoras endoteliales de la córnea humana que han sido cultivadas utilizando un medio de cultivo que contiene un inhibidor de ROCK, y en el que el contenido de factor de crecimiento epidérmico (EGF) es menor que una concentración que causará una transformación se recolectan en un tiempo en que se cumple cualquiera de o una pluralidad de las condiciones (a) ~ (d) que se indican a continuación, y se colocan en un estado de suspensión y luego de conservado. (a) Durante un período inmediatamente después de que una morfología de las células endoteliales de la córnea humana y/o de las células precursoras endoteliales de la córnea humana haya cambiado de una forma fusiforme a una forma poligonal o una forma elíptica cuya proporción eje mayor-eje menor es cercana a 1, hasta inmediatamente antes de que los límites entre las células se vuelvan indistintos. (b) Durante un período desde que un nivel de expresión de CD44 se vuelve igual o inferior a la mitad del valor máximo observado después del subcultivo más reciente hasta que este nivel de expresión alcanza una meseta. (c) Cuando la densidad celular de las células endoteliales de la córnea humana y/o de las células precursoras endoteliales de la córnea humana no sea inferior a 900 células/mm2 ni superior a 2500 células/mm2. (d) Cuando el número de días de cultivo desde el subcultivo más reciente no sea inferior a 4 días ni superior a 14 días.
MX2023004594A 2020-10-22 2021-10-19 Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas. MX2023004594A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020177634 2020-10-22
PCT/JP2021/038609 WO2022085680A1 (ja) 2020-10-22 2021-10-19 ヒト角膜内皮細胞及び／又はヒト角膜内皮前駆細胞の保存方法

Publications (1)

Publication Number Publication Date
MX2023004594A true MX2023004594A (es) 2023-05-08

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Application Number Title Priority Date Filing Date
MX2023004594A MX2023004594A (es) 2020-10-22 2021-10-19 Un metodo de almacenamiento de celulas endoteliales de la cornea humanas y/o celulas precursoras endoteliales de la cornea humanas.

Country Status (11)

Country Link
US (1) US20230380417A1 (es)
EP (1) EP4234682A4 (es)
JP (2) JP7361431B2 (es)
KR (1) KR20230091107A (es)
CN (1) CN116583593A (es)
AU (1) AU2021366805B2 (es)
CA (1) CA3196110A1 (es)
IL (1) IL302168A (es)
MX (1) MX2023004594A (es)
NZ (1) NZ799955A (es)
WO (1) WO2022085680A1 (es)

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4678783B1 (en) 1983-11-04 1995-04-04 Asahi Chemical Ind Substituted isoquinolinesulfonyl compounds
JPH0276977A (ja) 1988-09-12 1990-03-16 Matsushita Electric Ind Co Ltd 湯水混合装置
JPH0276976A (ja) 1988-09-12 1990-03-16 Matsushita Electric Ind Co Ltd 制御弁
JPH02100833A (ja) 1988-10-07 1990-04-12 Tokai T R W Kk 転造ダイスの加工方法
JPH0359913A (ja) 1989-07-28 1991-03-14 Yazaki Corp 金属溶射による回路体の製造方法
JPH0362227A (ja) 1989-07-31 1991-03-18 Nec Corp 高速再翻訳処理方式
JPH04620A (ja) 1990-04-18 1992-01-06 Nec Off Syst Ltd キーボード
JPH081403A (ja) 1994-06-22 1996-01-09 Fukui Seisakusho:Kk Ｎｃ装置付き工作機械によるネジ端部バリ取り方法
JP3906284B2 (ja) 1995-04-14 2007-04-18 株式会社東芝 光ディスクからビデオ及びオーディオを再生する再生システム、その再生方法及び光ディスクの記録方法
JP3421217B2 (ja) 1995-11-20 2003-06-30 麒麟麦酒株式会社 Ｒｈｏ標的タンパク質Ｒｈｏキナーゼ
JP4315407B2 (ja) 2000-09-07 2009-08-19 コスモ石油株式会社 競合見積管理システムおよび方法、並びに購買ウエブサーバ
JP2004009555A (ja) 2002-06-07 2004-01-15 Futec Inc 印刷欠点の検出装置および検出方法
US6911772B2 (en) 2002-06-12 2005-06-28 Eastman Kodak Company Oled display having color filters for improving contrast
JP4023271B2 (ja) 2002-09-20 2007-12-19 株式会社トヨトミ 窓用空気調和機の構造
JP2005003101A (ja) 2003-06-12 2005-01-06 Toyota Motor Corp 無段変速機の制御装置
JP2005035501A (ja) 2003-07-14 2005-02-10 Takahisa Tsutsui 油タンカー船の積付け計算をボタン操作によって調整かつ、連動して海面を表示するプログラム
JP2005035503A (ja) 2003-07-15 2005-02-10 Okubo Tomio 自転車等の防寒用ハンドルカバー
JP2005039564A (ja) 2003-07-16 2005-02-10 Nec Saitama Ltd 画像配信システム、サーバ装置、携帯端末装置及びそれに用いる画像配信方法
JP2005035506A (ja) 2003-07-17 2005-02-10 Masuo Kato パイプを使用した水上、水中液体貯蔵装置及び水上輸送方法
JP2005037197A (ja) 2003-07-18 2005-02-10 Ricoh Co Ltd 接触式表面形状測定装置及び測定方法
JP4160460B2 (ja) 2003-07-18 2008-10-01 ヤマザキマザック株式会社 レーザ加工機
JP2005037198A (ja) 2003-07-18 2005-02-10 Kawasaki Heavy Ind Ltd 内側炉心槽の挿入・引き抜き方法及びその装置と内側炉心槽引き抜き時の燃料集合体の拘束支持方法及びその装置
JP3759133B2 (ja) 2003-08-29 2006-03-22 ローム株式会社 電源装置
JP4315279B2 (ja) 2003-09-30 2009-08-19 能美防災株式会社 消防用設備の開閉弁装置及びその制御弁
JP2006057270A (ja) 2004-08-18 2006-03-02 Akimori Taniguchi 巨大水母類の漂着による、取水口スクリ−ン吸着閉塞防止システム。
US20060101035A1 (en) 2004-11-11 2006-05-11 Mustakallio Minna M System and method for blog functionality
US9248125B2 (en) 2007-08-29 2016-02-02 Senju Pharmaceutical Co., Ltd. Agent for promoting corneal endothelial cell adhesion
EP2894218B1 (en) * 2012-09-07 2018-09-12 JCR Pharmaceuticals Co., Ltd. Medium, for culturing corneal endothelial cells, containing conditioned medium from mesenchymal stem cells
JP6637656B2 (ja) * 2014-01-16 2020-01-29 千寿製薬株式会社 角膜内皮細胞を含有する移植用組成物
JP6574487B2 (ja) 2015-02-13 2019-09-11 ピー プラス エル ゲーエムベーハー アンド コー・カーゲー 工作機械における工作物の位置決定方法
WO2017110094A1 (ja) 2015-12-24 2017-06-29 学校法人同志社 カスパーゼ阻害剤を含む、ＴＧＦ－βに起因する障害を治療または予防するための医薬およびその応用
JP7008337B2 (ja) * 2016-02-15 2022-02-10 京都府公立大学法人 ヒト機能性角膜内皮細胞およびその応用
US20210195539A1 (en) 2018-08-09 2021-06-24 Sharp Kabushiki Kaisha Integrated access and backhaul node recognition in integrated access and backhaul network
JP2020032139A (ja) 2018-08-31 2020-03-05 株式会社三洋物産 遊技機

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JP2023139164A (ja) 2023-10-03
WO2022085680A1 (ja) 2022-04-28
NZ799955A (en) 2025-05-30
JP7361431B2 (ja) 2023-10-16
US20230380417A1 (en) 2023-11-30
AU2021366805B2 (en) 2025-04-24
JPWO2022085680A1 (es) 2022-04-28
KR20230091107A (ko) 2023-06-22
EP4234682A4 (en) 2024-11-27
AU2021366805A1 (en) 2023-06-22
EP4234682A1 (en) 2023-08-30
IL302168A (en) 2023-06-01
CA3196110A1 (en) 2022-04-28
CN116583593A (zh) 2023-08-11

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