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MX2017001596A - Enzima aglicosilada y usos de la misma. - Google Patents

Enzima aglicosilada y usos de la misma.

Info

Publication number
MX2017001596A
MX2017001596A MX2017001596A MX2017001596A MX2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A MX 2017001596 A MX2017001596 A MX 2017001596A
Authority
MX
Mexico
Prior art keywords
cellobiase
enzyme
aglycosylated
present
reesei
Prior art date
Application number
MX2017001596A
Other languages
English (en)
Inventor
Craig Masterman Thomas
Pangilinan Desiree
Yoshida Aiichiro
Landry Sean
Lynch James
KREDER Natasha
Medoff Marshall
Original Assignee
Xyleco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xyleco Inc filed Critical Xyleco Inc
Publication of MX2017001596A publication Critical patent/MX2017001596A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

La presente invencion se relaciona con composiciones que comprenden un polipeptido aglicosilado que tiene actividad de celobiasa y a metodos para la produccion y uso de las mismas. La presente invencion se basa, al menos en parte, en el descubrimiento sorprendente de que una celobiasa de Trichoderma reesei que fue expresada en una linea celular no fungica y aislada a partir de una celula huesped que no glicosila significativamente la enzima, tuvo actividad especifica mas alta en sustrato puro que la celobiasa endogena (glicosilada y secretada) de T. reesei.
MX2017001596A 2014-08-08 2015-08-07 Enzima aglicosilada y usos de la misma. MX2017001596A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462035346P 2014-08-08 2014-08-08
PCT/US2015/044136 WO2016022878A1 (en) 2014-08-08 2015-08-07 Aglycosylated enzyme and uses thereof

Publications (1)

Publication Number Publication Date
MX2017001596A true MX2017001596A (es) 2017-05-15

Family

ID=55264604

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017001596A MX2017001596A (es) 2014-08-08 2015-08-07 Enzima aglicosilada y usos de la misma.

Country Status (16)

Country Link
US (2) US10131894B2 (es)
EP (1) EP3177719A4 (es)
JP (1) JP2017524363A (es)
KR (1) KR20170040246A (es)
CN (1) CN106536730A (es)
AP (1) AP2016009640A0 (es)
AU (1) AU2015300897A1 (es)
BR (1) BR112017002391A2 (es)
CA (1) CA2955960A1 (es)
CU (1) CU20170011A7 (es)
EA (1) EA201790054A1 (es)
IL (1) IL250272A0 (es)
MX (1) MX2017001596A (es)
PH (1) PH12016502604A1 (es)
SG (1) SG11201610894UA (es)
WO (1) WO2016022878A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10131894B2 (en) * 2014-08-08 2018-11-20 Xyleco, Inc. Aglycosylated enzyme and uses thereof
CN115806950A (zh) * 2022-11-24 2023-03-17 山东省科学院生物研究所 嗜热纤维二糖脱氢酶及其去糖基化酶和作为生物传感元件中的应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5028515B2 (es) 1971-09-30 1975-09-16
US6103464A (en) * 1990-12-10 2000-08-15 Genencor International, Inc. Method of detecting DNA encoding a β-glucosidase from a filamentous fungus
AU784258B2 (en) 1999-11-19 2006-03-02 Yissum Research Development Company Of The Hebrew University Of Jerusalem Aspegillus niger beta-glucosidase gene, protein and uses thereof
US6946277B2 (en) 2001-01-31 2005-09-20 Council Of Scientific And Industrial Research Method for enhancing cellobiase activity of termitomyces clypeatus using a glycosylation inhibitor
FI120045B (fi) * 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
CA2658483A1 (en) 2006-07-21 2008-01-24 Xyleco, Inc. Conversion systems for biomass
CN101516906B (zh) * 2006-07-21 2013-11-06 诺维信股份有限公司 提高具有生物学活性之多肽的分泌的方法
EP2037274A1 (de) * 2007-09-11 2009-03-18 GALAB Technologies GmbH Lektinbasierter Glykan-Assay
FR2935986B1 (fr) * 2008-09-12 2012-11-23 Inst Francais Du Petrole Variants de beta-glucosidase a activite amelioree et leurs utilisations.
WO2010135380A1 (en) 2009-05-20 2010-11-25 Xyleco, Inc. Processing hydrocarbon-containing materials
EP2599861B1 (en) * 2009-11-20 2017-08-02 Danisco US Inc. Beta-glucosidase variants with improved properties
KR20180130585A (ko) 2010-07-19 2018-12-07 질레코 인코포레이티드 바이오매스의 가공처리
MY159993A (en) 2010-10-20 2017-02-15 Xyleco Inc Method for treating lignocellulosic material by irradiating with an electron beam
CA2829918A1 (en) 2011-03-17 2012-09-20 Danisco Us Inc. Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
AU2012358374B2 (en) 2011-12-22 2016-05-19 Xyleco, Inc. Processing biomass
FR2994981B1 (fr) * 2012-09-05 2014-09-12 Centre Nat Rech Scient Polypeptide a activite beta-glucosidase renforcee a basse temperature
EP2929028A1 (en) 2012-12-07 2015-10-14 Novozymes A/S Method for generating site-specific mutations in filamentous fungi
US10131894B2 (en) 2014-08-08 2018-11-20 Xyleco, Inc. Aglycosylated enzyme and uses thereof

Also Published As

Publication number Publication date
US20170137797A1 (en) 2017-05-18
CA2955960A1 (en) 2016-02-11
CU20170011A7 (es) 2017-08-08
CN106536730A (zh) 2017-03-22
JP2017524363A (ja) 2017-08-31
BR112017002391A2 (pt) 2017-11-28
AP2016009640A0 (en) 2016-12-31
AU2015300897A1 (en) 2017-01-05
EP3177719A1 (en) 2017-06-14
EP3177719A4 (en) 2018-04-04
US20190024068A1 (en) 2019-01-24
IL250272A0 (en) 2017-03-30
EA201790054A1 (ru) 2017-07-31
US10131894B2 (en) 2018-11-20
KR20170040246A (ko) 2017-04-12
SG11201610894UA (en) 2017-01-27
WO2016022878A1 (en) 2016-02-11
PH12016502604A1 (en) 2017-04-24

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