[go: up one dir, main page]

LT3299469T - Sistemos ir būdai, skirti nukleorūgšties identifikavimo žymės įvedimui - Google Patents

Sistemos ir būdai, skirti nukleorūgšties identifikavimo žymės įvedimui

Info

Publication number
LT3299469T
LT3299469T LTEP17198030.3T LT17198030T LT3299469T LT 3299469 T LT3299469 T LT 3299469T LT 17198030 T LT17198030 T LT 17198030T LT 3299469 T LT3299469 T LT 3299469T
Authority
LT
Lithuania
Prior art keywords
systems
methods
nucleic acid
barcoding nucleic
barcoding
Prior art date
Application number
LTEP17198030.3T
Other languages
English (en)
Inventor
David A. Weitz
Allon Moshe Klein
Ilke Akartuna
Linas Mazutis
Marc W. Kirschner
Original Assignee
President And Fellows Of Harvard College
Vilnius University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College, Vilnius University filed Critical President And Fellows Of Harvard College
Publication of LT3299469T publication Critical patent/LT3299469T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
LTEP17198030.3T 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgšties identifikavimo žymės įvedimui LT3299469T (lt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461982001P 2014-04-21 2014-04-21
US201462065348P 2014-10-17 2014-10-17
US201462066188P 2014-10-20 2014-10-20
US201462072944P 2014-10-30 2014-10-30

Publications (1)

Publication Number Publication Date
LT3299469T true LT3299469T (lt) 2020-04-27

Family

ID=54333034

Family Applications (3)

Application Number Title Priority Date Filing Date
LTEP17198030.3T LT3299469T (lt) 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgšties identifikavimo žymės įvedimui
LTEP15783629.7T LT3134536T (lt) 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui
LTEP18201501.6T LT3456846T (lt) 2014-04-21 2015-04-17 Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai

Family Applications After (2)

Application Number Title Priority Date Filing Date
LTEP15783629.7T LT3134536T (lt) 2014-04-21 2015-04-17 Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui
LTEP18201501.6T LT3456846T (lt) 2014-04-21 2015-04-17 Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai

Country Status (9)

Country Link
EP (3) EP3456846B1 (lt)
JP (1) JP6853667B2 (lt)
CN (1) CN107075543B (lt)
AU (1) AU2015250034B2 (lt)
CA (1) CA2946144A1 (lt)
DK (3) DK3134536T3 (lt)
HK (1) HK1253013A1 (lt)
LT (3) LT3299469T (lt)
WO (1) WO2015164212A1 (lt)

Families Citing this family (149)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1663497B2 (en) 2003-09-05 2020-03-25 Stokes Bio Limited A microfluidic analysis system
US9597644B2 (en) * 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
EP1981624B1 (en) 2006-02-07 2011-09-07 Stokes Bio Limited A liquid bridge system and method
EP2530167A1 (en) 2006-05-11 2012-12-05 Raindance Technologies, Inc. Microfluidic Devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
DE202012013668U1 (de) 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
EP3161157B1 (en) * 2014-06-24 2024-03-27 Bio-Rad Laboratories, Inc. Digital pcr barcoding
AU2015279548B2 (en) 2014-06-26 2020-02-27 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3191605B1 (en) * 2014-09-09 2022-07-27 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
JP2017532042A (ja) 2014-10-29 2017-11-02 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
BR112017014902A2 (pt) 2015-01-12 2018-03-13 10X Genomics Inc processos e sistemas para a preparação de bibliotecas de sequenciamento de ácido nucleico e bibliotecas preparadas usando os mesmos
JP2018505688A (ja) 2015-02-24 2018-03-01 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列包括度（ｃｏｖｅｒａｇｅ）のための方法
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
EP3268125A4 (en) 2015-03-13 2018-08-15 President and Fellows of Harvard College Determination of cells using amplification
EP3283629A4 (en) 2015-04-17 2018-08-29 President and Fellows of Harvard College Barcoding systems and methods for gene sequencing and other applications
WO2017079593A1 (en) * 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
WO2017083375A1 (en) * 2015-11-10 2017-05-18 Illumina, Inc. Inertial droplet generation and particle encapsulation
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
ES2926495T3 (es) 2015-12-04 2022-10-26 10X Genomics Inc Métodos y composiciones para el análisis de ácidos nucleicos
CN117512080A (zh) * 2015-12-07 2024-02-06 生物-拉德实验室公司 在分配中使用微粒的多路技术
EP3263715B1 (en) 2016-06-28 2020-01-08 Hifibio Method for transcriptome analysis of single cells
CN108779491B (zh) 2016-02-11 2021-03-09 10X基因组学有限公司 用于全基因组序列数据的从头组装的系统、方法和介质
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
CN110199019B (zh) 2016-05-02 2024-09-10 Encodia有限公司 采用核酸编码的大分子分析
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2017218486A1 (en) * 2016-06-14 2017-12-21 Mission Bio, Inc. Methods and compositions for emulsification of solid supports in deformable beads
EP3260201A1 (en) 2016-06-21 2017-12-27 Hifibio Microfluidic filtration unit and related microfluidic system
WO2018096058A1 (en) * 2016-11-23 2018-05-31 Universite De Strasbourg Tandem barcoding of target molecules for their absolute quantification at single-entity resolution
AU2017382905A1 (en) * 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) * 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018156935A1 (en) 2017-02-24 2018-08-30 The Regents Of The University Of California Particle-drop structures and methods for making and using the same
ES2922624T3 (es) * 2017-03-13 2022-09-19 Gigagen Inc Sistemas y métodos para el análisis combinatorio paralelo masivo de células individuales
LT3375889T (lt) 2017-03-17 2020-06-25 Hifibio Sas Vienos ląstelės analizė
CN110678558B (zh) * 2017-05-02 2023-06-02 国立大学法人东京大学 整体性检测单个细胞的非破坏性测量信息和基因组相关信息的方法
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
EP4435113A1 (en) 2017-05-18 2024-09-25 10x Genomics, Inc. Methods and systems for sorting droplets and beads
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
AU2018273999A1 (en) 2017-05-26 2019-12-05 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
WO2019036505A1 (en) * 2017-08-14 2019-02-21 Mission Bio, Inc. METHODS AND COMPOSITIONS FOR EVALUATING THE UNIFORMITY OF AN EMULSION
US10821442B2 (en) 2017-08-22 2020-11-03 10X Genomics, Inc. Devices, systems, and kits for forming droplets
US20200292526A1 (en) 2017-09-07 2020-09-17 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
RU2756306C1 (ru) * 2017-09-22 2021-09-29 федеральное государственное автономное образовательное учреждение высшего образования "Тюменский государственный университет" Система и способ для анализа фенотипа и полинуклеотидного секвенирования биологических частиц с использованием детерминированного штрихкодирования
CN116785253A (zh) * 2017-09-29 2023-09-22 加利福尼亚大学董事会 单分散乳液的制备方法
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
EP3695008B1 (en) * 2017-10-09 2021-11-24 Psomagen, Inc. Single molecule sequencing and unique molecular identifiers to characterize nucleic acid sequences
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
EP3821032A1 (en) * 2018-07-13 2021-05-19 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
US20210310063A1 (en) * 2018-07-13 2021-10-07 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
CN119224296A (zh) * 2018-09-26 2024-12-31 北卡罗来纳-查佩尔山大学 用于改进测定的化合物、组合物和方法
ES2992313T3 (es) * 2018-09-28 2024-12-11 Fluent Biosciences Inc Captura de dianas en gotitas monodispersas
US20220010373A1 (en) * 2018-11-07 2022-01-13 The University Of Tokyo Method for detecting genome-related information of cell coexisting with at least one type of test substance
US11512356B2 (en) 2018-11-08 2022-11-29 Tokitae Llc Systems and methods for particle multiplexing in droplets
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP4464778A3 (en) 2018-12-13 2025-02-19 DNA Script Direct oligonucleotide synthesis on cdna
CN109851711B (zh) * 2018-12-29 2020-09-25 苏州绘真生物科技有限公司 可溶性水凝胶微球及其制备方法与在单细胞检测中的应用
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
EP3938539A4 (en) * 2019-03-12 2022-12-14 Universal Sequencing Technology METHOD OF INTRACELLULAR SINGLE CELL TRACTION AND ITS APPLICATIONS
WO2020206285A1 (en) * 2019-04-05 2020-10-08 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
EP3973074A4 (en) 2019-05-22 2023-09-06 Mission Bio, Inc. METHOD AND DEVICE FOR SIMULTANEOUS TARGETED SEQUENCING OF DNA, RNA AND PROTEIN
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
WO2021046475A1 (en) * 2019-09-06 2021-03-11 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
CN111172257A (zh) * 2020-01-16 2020-05-19 南方科技大学 一种带编码的凝胶微粒及其制备方法和应用
WO2021147069A1 (zh) * 2020-01-23 2021-07-29 青岛华大智造普惠科技有限公司 基于液滴微流控的单细胞测序及应用
CA3153296A1 (en) * 2020-03-24 2021-09-30 Alex Chenchik Single cell genetic analysis
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20250034640A1 (en) * 2021-04-20 2025-01-30 Simsen Diagnostics Ab Compositions and methods for cell-free nucleic acid isolation
CN113215233A (zh) * 2021-05-15 2021-08-06 墨卓生物科技(浙江)有限公司 一种用于单细胞测序的具有特定寡核苷酸序列的微球
WO2022256612A1 (en) * 2021-06-04 2022-12-08 Enumerix, Inc. Compositions, methods, and systems for single cell barcoding and sequencing
CN117794646A (zh) 2021-06-04 2024-03-29 伊努梅里斯公司 用于单细胞条形码化和测序的组合物、方法和系统
CN113584598A (zh) * 2021-06-17 2021-11-02 南方科技大学 一种单细胞文库的构建方法
EP4392577A1 (en) * 2021-08-24 2024-07-03 Ecole Polytechnique Federale De Lausanne (Epfl) Optimised set of oligonucleotides for bulk rna barcoding and sequencing
CN113889192B (zh) * 2021-09-29 2024-02-27 西安热工研究院有限公司 一种基于深层降噪自编码器的单细胞RNA-seq数据聚类方法
WO2023099667A1 (en) * 2021-12-01 2023-06-08 Vilnius University Methods for processing and barcoding nucleic acids
CN114177960B (zh) * 2021-12-15 2023-04-28 天津大学 基于超高频体声波谐振器的微液滴操纵方法以及装置
WO2023139272A1 (en) * 2022-01-24 2023-07-27 Scipio Bioscience Gelation device with piston
WO2023237180A1 (en) * 2022-06-07 2023-12-14 Alithea Genomics Sa Optimised set of oligonucleotides for bulk rna barcoding and sequencing
WO2024011226A1 (en) * 2022-07-07 2024-01-11 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cell-barcode recorder devices and methods
EP4331724A1 (en) * 2022-08-29 2024-03-06 Institut Curie Method of extracting a bead from a droplet
EP4435114A1 (en) 2023-03-24 2024-09-25 Miltenyi Biotec B.V. & Co. KG Syntheis of highly multiplexed oligo-barcoded fluorescent beads for single cell seq applications

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
CA2784762A1 (en) 2003-04-10 2004-10-28 President And Fellows Of Harvard College Formation and control of fluidic species
BRPI0414004A (pt) 2003-08-27 2006-10-24 Harvard College controle eletrÈnico de espécies fluìdicas
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
DE602007009811D1 (de) 2006-01-27 2010-11-25 Harvard College Koaleszenz fluider tröpfchen
MX2009009127A (es) * 2007-02-27 2009-10-19 Univ Northwestern Unión de moléculas a nanopartículas.
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20090181375A1 (en) * 2008-01-11 2009-07-16 Peter Brian J Method for detection of nucleic acid barcodes
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3587594B1 (en) 2008-12-19 2022-04-13 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
KR101770363B1 (ko) * 2009-04-02 2017-08-22 플루이다임 코포레이션 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법
US9757698B2 (en) 2009-06-26 2017-09-12 President And Fellows Of Harvard College Fluid injection
US9056289B2 (en) 2009-10-27 2015-06-16 President And Fellows Of Harvard College Droplet creation techniques
US8586301B2 (en) * 2010-06-30 2013-11-19 Stratos Genomics, Inc. Multiplexed identification of nucleic acid sequences
CA2814049C (en) * 2010-10-08 2021-07-13 President And Fellows Of Harvard College High-throughput single cell barcoding
WO2012083225A2 (en) * 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
EP3736281A1 (en) * 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
JP5822929B2 (ja) * 2011-07-19 2015-11-25 株式会社日立ハイテクノロジーズ 核酸分析装置
JP6196241B2 (ja) * 2012-02-02 2017-09-13 インベンラ， インコーポレイテッド 生物学的に活性なポリペプチドのハイスループットスクリーニング
EP2823064B1 (en) * 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
JP6558830B2 (ja) * 2012-06-15 2019-08-14 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム 複数転写産物のハイスループットシークエンシング
EP2898096B1 (en) * 2012-09-21 2024-02-14 The Broad Institute, Inc. Methods for labeling of rnas
DK3089822T3 (da) * 2013-12-30 2022-05-02 Atreca Inc Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder
EP3161157B1 (en) * 2014-06-24 2024-03-27 Bio-Rad Laboratories, Inc. Digital pcr barcoding

Also Published As

Publication number Publication date
LT3134536T (lt) 2020-05-11
CA2946144A1 (en) 2015-10-29
EP3299469B1 (en) 2020-03-04
AU2015250034A1 (en) 2016-11-03
EP3134536A1 (en) 2017-03-01
WO2015164212A1 (en) 2015-10-29
EP3456846A1 (en) 2019-03-20
AU2015250034B2 (en) 2019-06-20
EP3134536A4 (en) 2017-12-20
EP3456846B1 (en) 2022-06-22
CN107075543B (zh) 2021-11-16
WO2015164212A9 (en) 2016-10-13
DK3134536T3 (da) 2020-02-03
HK1253013A1 (zh) 2019-06-06
JP6853667B2 (ja) 2021-03-31
LT3456846T (lt) 2022-09-12
EP3299469A1 (en) 2018-03-28
EP3134536B1 (en) 2019-12-18
DK3456846T3 (da) 2022-07-11
JP2017515469A (ja) 2017-06-15
DK3299469T3 (da) 2020-03-30
CN107075543A (zh) 2017-08-18

Similar Documents

Publication Publication Date Title
HK1253013A1 (zh) 用於條形碼化核酸的系統和方法
HK1244554A1 (zh) 用於智能空間的系統和方法
IL249461B (en) Processes and systems for constructing a nucleic acid sequence
DK3134131T3 (en) Nucleic acid vaccines
SG11201609892RA (en) Transport-container system and method
EP3160661C0 (en) SYSTEM AND PROCESS FOR BIOROT INHIBITION
SG10201405182WA (en) Method and system
EP3132060A4 (en) Methods and systems for nucleic acid amplification
GB201421814D0 (en) System and method
GB201408841D0 (en) Nucleic acid processing
GB201405226D0 (en) Nucleic acid preparation method
SG11201703181YA (en) Identification system and method
GB201513128D0 (en) Nucleic acid detection method
PL3183174T3 (pl) Układ do pipetowania
GB201420186D0 (en) Identification system and method
GB201523060D0 (en) Speed-limit-compliance system and method
GB201409786D0 (en) Nucleic acid amplification system
GB201415688D0 (en) Method and Systems
GB201404184D0 (en) Method and system
GB201417092D0 (en) Systems and methods for state connections
GB2528665B (en) Identification System
GB201521454D0 (en) Nucleic acid aditing systems
GB201410223D0 (en) Nucleic acid amplification system
GB201402370D0 (en) Nucleic acid amplification system
GB201415979D0 (en) System and method