[go: up one dir, main page]

DK3089822T3 - Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder - Google Patents

Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder Download PDF

Info

Publication number
DK3089822T3
DK3089822T3 DK14830928.9T DK14830928T DK3089822T3 DK 3089822 T3 DK3089822 T3 DK 3089822T3 DK 14830928 T DK14830928 T DK 14830928T DK 3089822 T3 DK3089822 T3 DK 3089822T3
Authority
DK
Denmark
Prior art keywords
analysis
individual cells
bar codes
nucleic acid
acids associated
Prior art date
Application number
DK14830928.9T
Other languages
English (en)
Inventor
Gary Withey
Yann Chong Tan
Original Assignee
Atreca Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atreca Inc filed Critical Atreca Inc
Application granted granted Critical
Publication of DK3089822T3 publication Critical patent/DK3089822T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DK14830928.9T 2013-12-30 2014-12-30 Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder DK3089822T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361922012P 2013-12-30 2013-12-30
PCT/US2014/072898 WO2015103339A1 (en) 2013-12-30 2014-12-30 Analysis of nucleic acids associated with single cells using nucleic acid barcodes

Publications (1)

Publication Number Publication Date
DK3089822T3 true DK3089822T3 (da) 2022-05-02

Family

ID=52424112

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14830928.9T DK3089822T3 (da) 2013-12-30 2014-12-30 Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder

Country Status (13)

Country Link
US (7) US9580736B2 (da)
EP (2) EP3089822B1 (da)
JP (1) JP6608368B2 (da)
KR (2) KR102433825B1 (da)
CN (2) CN106460033B (da)
AU (1) AU2014373757B2 (da)
CA (1) CA2935122C (da)
DK (1) DK3089822T3 (da)
ES (1) ES2912183T3 (da)
PL (1) PL3089822T3 (da)
PT (1) PT3089822T (da)
SG (2) SG11201605344YA (da)
WO (1) WO2015103339A1 (da)

Families Citing this family (177)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
EP2854057B1 (en) 2010-05-18 2018-03-07 Natera, Inc. Methods for non-invasive pre-natal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
AU2012212148B8 (en) 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
EP2673729B1 (en) 2011-02-09 2018-10-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9644204B2 (en) 2013-02-08 2017-05-09 10X Genomics, Inc. Partitioning and processing of analytes and other species
DK2970951T3 (da) 2013-03-13 2019-05-13 Illumina Inc Fremgangsmåder til nukleinsyresekventering
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
WO2015054292A1 (en) 2013-10-07 2015-04-16 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
WO2015095226A2 (en) 2013-12-20 2015-06-25 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic dna samples
PL3089822T3 (pl) * 2013-12-30 2022-09-19 Atreca, Inc. Analiza kwasów nukleinowych powiązanych z pojedynczymi komórkami z użyciem kodów kreskowych kwasów nukleinowych
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
JP6853667B2 (ja) * 2014-04-21 2021-03-31 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸をバーコーディングするためのシステムおよび方法
JP6659575B2 (ja) 2014-04-21 2020-03-04 ナテラ， インコーポレイテッド 変異の検出および染色体分節の倍数性
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
JP6412954B2 (ja) * 2014-04-29 2018-10-24 イルミナ インコーポレイテッド 鋳型切換え及びタグメンテーションを用いる単一細胞の遺伝子発現の多重分析
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
JP2017522866A (ja) 2014-06-26 2017-08-17 １０エックス ジェノミクス， インコーポレイテッド 核酸配列の分析
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
SG10201911069WA (en) * 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
IL299976B2 (en) 2014-10-17 2024-11-01 Illumina Cambridge Ltd Contiguity preserving transposition
DK3207134T3 (da) * 2014-10-17 2019-09-23 Illumina Cambridge Ltd Kontiguitetsbevarende transposition
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
ES2824700T3 (es) 2015-02-19 2021-05-13 Becton Dickinson Co Análisis unicelular de alto rendimiento que combina información proteómica y genómica
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US20160257993A1 (en) * 2015-02-27 2016-09-08 Cellular Research, Inc. Methods and compositions for labeling targets
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
WO2016168584A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
EP4582556A2 (en) 2015-04-23 2025-07-09 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
HK1250757A1 (zh) * 2015-08-28 2019-01-11 Illumina, Inc. 來自單細胞的核酸序列分析
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
MX2018003532A (es) 2015-09-24 2019-04-25 Abvitro Llc Conjugados de afinidad-oligonucleotido y usos de los mismos.
CN108473984A (zh) * 2015-11-04 2018-08-31 阿特雷卡公司 用于分析与单个细胞相关联的核酸的核酸条形码的组合组
US20170141793A1 (en) * 2015-11-13 2017-05-18 Microsoft Technology Licensing, Llc Error correction for nucleotide data stores
EP4524255A3 (en) 2015-12-04 2025-04-02 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
US11965891B2 (en) * 2015-12-30 2024-04-23 Bio-Rad Laboratories, Inc. Digital protein quantification
US12083512B2 (en) * 2016-01-14 2024-09-10 European Molecular Biology Laboratory Microfluidic analysis of ligand induced cell expression
WO2017181146A1 (en) 2016-04-14 2017-10-19 Guardant Health, Inc. Methods for early detection of cancer
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
US11702702B2 (en) * 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3464633A4 (en) * 2016-05-25 2020-01-01 Bio-Rad Laboratories, Inc. DIGITAL PROXIMITY TEST
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP4407625A3 (en) 2016-05-26 2024-10-23 Becton, Dickinson and Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN105950612B (zh) * 2016-07-08 2019-06-21 北京全式金生物技术有限公司 一种高效的dna接头连接方法
CN109642219B (zh) 2016-08-05 2021-02-26 生物辐射实验室股份有限公司 第二链引导
CN116445593A (zh) * 2016-08-10 2023-07-18 格里尔公司 测定一生物样品的一甲基化图谱的方法
AU2017331459B2 (en) 2016-09-26 2023-04-13 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
WO2018075693A1 (en) * 2016-10-19 2018-04-26 10X Genomics, Inc. Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
US11667951B2 (en) 2016-10-24 2023-06-06 Geneinfosec, Inc. Concealing information present within nucleic acids
EP3538672B1 (en) 2016-11-08 2025-07-16 Becton, Dickinson and Company Methods for cell label classification
WO2018089378A1 (en) 2016-11-08 2018-05-17 Cellular Research, Inc. Methods for expression profile classification
JP7050057B2 (ja) * 2016-11-10 2022-04-07 タカラ バイオ ユーエスエー， インコーポレイテッド 増幅二本鎖デオキシリボ核酸の作製方法ならびに該方法で使用する組成物及びキット
US10011870B2 (en) * 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20180195115A1 (en) * 2016-12-22 2018-07-12 Illumina, Inc. Arrays with quality control tracers
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
EP3375889B1 (en) 2017-03-17 2019-12-11 HiFiBiO SAS Single cell analysis
CN110546272B (zh) * 2017-04-14 2024-06-07 夸登特健康公司 将衔接子附接至样品核酸的方法
RU2761479C2 (ru) * 2017-04-21 2021-12-08 Меса Байотек, Инк. Флюидная кассета для тестирования
CN110168087B (zh) * 2017-04-27 2023-11-14 深圳华大基因股份有限公司 Dna标签及其应用
WO2018203576A1 (ja) 2017-05-02 2018-11-08 国立大学法人 東京大学 一細胞の非破壊的計測情報とゲノム関連情報とを一体的に検出する方法
AU2018262331A1 (en) * 2017-05-05 2019-11-21 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
CN107502607A (zh) * 2017-06-20 2017-12-22 浙江大学 一种大量组织、细胞样本mRNA的分子条形码标记、文库构建、测序的方法
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
EP4241882B1 (en) * 2017-10-27 2025-04-30 10X Genomics, Inc. Methods for sample preparation and analysis
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US20190153438A1 (en) * 2017-11-15 2019-05-23 Viome, Inc. Methods and compositions for preparing polynucleotide libraries
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
US20210371853A1 (en) * 2017-12-09 2021-12-02 Viome, Inc. Methods for nucleic acid library creation
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
CN111492068B (zh) * 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
KR102014470B1 (ko) 2017-12-29 2019-08-26 한국과학기술원 핵산의 절단 및 중합연쇄반응 시스템 기반 등온 핵산증폭기술을 이용한 표적 rna 검출 방법
WO2019133874A1 (en) * 2017-12-31 2019-07-04 Berkeley Lights, Inc. General functional assay
WO2019147663A1 (en) * 2018-01-24 2019-08-01 Freenome Holdings, Inc. Methods and systems for abnormality detection in the patterns of nucleic acids
SG11202007686VA (en) * 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
CN112074610A (zh) * 2018-02-22 2020-12-11 10X基因组学有限公司 接合介导的核酸分析
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US20210087549A1 (en) * 2018-02-23 2021-03-25 Yale University Single-cell freeze-thaw lysis
CN111836890A (zh) * 2018-03-07 2020-10-27 威斯康星校友研究基金会 单细胞的高通量核酸谱分析
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
JP7573443B2 (ja) 2018-04-14 2024-10-25 ナテラ， インコーポレイテッド 循環腫瘍ｄｎａの個別化された検出を用いる癌検出およびモニタリングの方法
EP4234717A3 (en) 2018-05-03 2023-11-01 Becton, Dickinson and Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
KR102640255B1 (ko) 2018-05-17 2024-02-27 일루미나, 인코포레이티드 감소된 증폭 편향을 갖는 고속-대량 단일 세포 서열분석
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
JP2021531750A (ja) 2018-07-12 2021-11-25 ボード オブ リージェンツ, ザ ユニバーシティ オブ テキサス システムBoard Of Regents, The University Of Texas System オリゴヌクレオチドによる分子近傍検出
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3844299A1 (en) * 2018-08-28 2021-07-07 Becton Dickinson and Company Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
EP4471156A3 (en) 2018-10-01 2025-02-26 Becton, Dickinson and Company Determining 5' transcript sequences
CA3117476A1 (en) * 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
EP3878936A4 (en) 2018-11-07 2022-08-10 The University of Tokyo METHOD FOR DETECTING INFORMATION RELATING TO THE GENOME OF A CELL COEXISTING WITH ONE OR MORE SUBSTANCES TO BE DETECTED
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
EP3891303A4 (en) * 2018-12-07 2022-09-14 Qiagen Sciences LLC Methods for preparing cdna samples for rna sequencing, and cdna samples and uses thereof
US11459607B1 (en) * 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
US11358137B2 (en) 2018-12-26 2022-06-14 Industrial Technology Research Institute Tubular structure for producing droplets and method for producing droplets
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
CN120099139A (zh) 2019-02-14 2025-06-06 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN110241460A (zh) * 2019-05-31 2019-09-17 南方医科大学南方医院 一种甄别独立样品自身交叉反应的免疫组库方法
WO2020247263A1 (en) 2019-06-06 2020-12-10 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
BR112022002805A2 (pt) * 2019-08-14 2022-08-09 Irepertoire Inc Método de captura por sonda para recuperação de vdj da cadeia alfa e beta de tcr por rna transcrito de forma reversa baseado em óligo-dt
EP4041310A4 (en) 2019-10-10 2024-05-15 1859, Inc. MICROFLUIDIC SCREENING METHODS AND SYSTEMS
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
EP4089181A4 (en) * 2020-01-10 2024-05-22 Thinkcyte, Inc. Novel cellular phenotype screening method
JP2023511278A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞遺伝子プロファイリングのための方法およびシステム
CN115698282A (zh) * 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
CA3167729A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
US20230220376A1 (en) 2020-01-22 2023-07-13 Atreca, Inc. High throughput linking of multiple transcripts
CA3165999A1 (en) * 2020-01-24 2021-07-29 Marion Francis SETLIFF Methods for identification of ligand-blocking antibodies and for determining antibody potency
EP4471155A3 (en) 2020-01-29 2024-12-18 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4182477A4 (en) * 2020-07-15 2024-09-11 Fluent Biosciences Inc. MULTI-LEVEL LIGATION OLIGOS
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
AU2021401424A1 (en) 2020-12-18 2023-06-29 Ionis Pharmaceuticals, Inc. Compounds and methods for modulating factor xii
US20240042442A1 (en) * 2020-12-22 2024-02-08 Hifibio Sas Microfluidic methods and systems
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
AU2022389197A1 (en) * 2021-11-16 2024-05-02 Pioneer Hi-Bred International, Inc. Maize event das-01131-3 and methods for detection thereof
EP4353825A1 (en) * 2022-10-10 2024-04-17 bisy GmbH Modified promoter sequences
WO2025085431A1 (en) * 2023-10-16 2025-04-24 Bio-Rad Laboratories, Inc. Enzymatic selection of nucleic acid

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5624711A (en) * 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
DE60221036T2 (de) 2001-02-23 2007-10-11 Japan Science And Technology Agency, Kawaguchi Vorrichtung zum Erzeugen von Emulsionen und Mikrokapseln
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US7976779B2 (en) * 2002-06-26 2011-07-12 California Institute Of Technology Integrated LC-ESI on a chip
US7670810B2 (en) 2003-06-20 2010-03-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
CA2560272C (en) 2004-03-23 2012-05-08 Japan Science And Technology Agency Method and apparatus for producing microdroplets
JOP20080381B1 (ar) 2007-08-23 2023-03-28 Amgen Inc بروتينات مرتبطة بمولدات مضادات تتفاعل مع بروبروتين كونفيرتاز سيتيليزين ككسين من النوع 9 (pcsk9)
ES2391906T3 (es) * 2007-09-12 2012-11-30 Institut Pasteur Polinucleótido apto para un ensayo indicador basado en una sola célula para controlar los patrones de expresión génica con una resolución espacio-temporal elevada
US20100179075A1 (en) * 2008-07-30 2010-07-15 Life Technologies Corporation Particles for use in supported nucleic acid ligation and detection sequencing
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
EP2420579A1 (en) * 2010-08-17 2012-02-22 QIAGEN GmbH Helicase dependent isothermal amplification using nicking enzymes
WO2012033687A1 (en) * 2010-09-10 2012-03-15 New England Biolabs, Inc. Method for reducing adapter-dimer formation
EP2625295B1 (en) 2010-10-08 2019-03-13 President and Fellows of Harvard College High-throughput immune sequencing
GB2512213B (en) * 2010-10-08 2015-02-11 Harvard College High-throughput single cell barcoding
WO2012083225A2 (en) 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
US9150852B2 (en) * 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
WO2012150317A1 (en) * 2011-05-05 2012-11-08 Institut National De La Sante Et De La Recherche Medicale (Inserm) Linear dna amplification
AU2012249158B2 (en) 2011-04-28 2017-06-29 Department Of Veterans Affairs Identification of polynucleotides associated with a sample
US20150125865A1 (en) 2011-12-23 2015-05-07 Gigagen, Inc. Methods And Apparatuses For Droplet Mixing
US9176031B2 (en) * 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
LT3305918T (lt) * 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
NO2694769T3 (da) * 2012-03-06 2018-03-03
JP2015519900A (ja) * 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
EP2861760B1 (en) * 2012-06-15 2020-01-01 The Board of Regents of The University of Texas System High throughput sequencing of multiple transcripts of a single cell
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
CN115558661A (zh) * 2013-08-23 2023-01-03 路德维格癌症研究院 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物
GB201317301D0 (en) * 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
PL3089822T3 (pl) 2013-12-30 2022-09-19 Atreca, Inc. Analiza kwasów nukleinowych powiązanych z pojedynczymi komórkami z użyciem kodów kreskowych kwasów nukleinowych
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids

Also Published As

Publication number Publication date
EP3089822B1 (en) 2022-04-06
CN106460033B (zh) 2021-12-24
KR20160108377A (ko) 2016-09-19
AU2014373757A1 (en) 2016-07-07
SG11201605344YA (en) 2016-07-28
PL3089822T3 (pl) 2022-09-19
KR20220119751A (ko) 2022-08-30
US20220243240A1 (en) 2022-08-04
ES2912183T3 (es) 2022-05-24
KR102433825B1 (ko) 2022-08-31
US20150329891A1 (en) 2015-11-19
CN114717291A (zh) 2022-07-08
US20220389471A1 (en) 2022-12-08
US10316345B2 (en) 2019-06-11
US9580736B2 (en) 2017-02-28
CA2935122A1 (en) 2015-07-09
CA2935122C (en) 2023-09-19
US20220389472A1 (en) 2022-12-08
CN106460033A (zh) 2017-02-22
EP3089822A1 (en) 2016-11-09
JP6608368B2 (ja) 2019-11-20
PT3089822T (pt) 2022-05-06
US20250059576A1 (en) 2025-02-20
US20170369921A1 (en) 2017-12-28
AU2014373757B2 (en) 2019-12-12
US20200123582A1 (en) 2020-04-23
SG10201807112XA (en) 2018-09-27
JP2017506877A (ja) 2017-03-16
EP4094834A1 (en) 2022-11-30
WO2015103339A1 (en) 2015-07-09

Similar Documents

Publication Publication Date Title
DK3089822T3 (da) Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder
DK3620534T3 (da) Crispr-cas sammensætninger af nucleinsyre-targeting nucleinsyrer
GB2525104B (en) Massively Parallel Single Cell Nucleic Acid Analysis
BR112014025695A2 (pt) Preparação de amostra de ácido nucleico
DK3452591T5 (da) Makromolekyleanalyse under anvendelse af nukleinsyrekodning
DK3341482T3 (da) Kunstige nukleinsyremolekyler
LT3334841T (lt) Nukleorūgščių tyrimo būdai
SI3294325T1 (sl) Krepitev učinka car-izdelanih t celic s cepljenjem z nukleinsko kislino
HRP20182030T1 (hr) Supstituirani nukleozidi, nukleotidi i njihovi analozi
EP2673380A4 (en) ANALYSIS OF NUCLEIC ACIDS
IL239324B (en) Substituted nucleosides, nucleotides and analogs thereof
SG11201603144QA (en) Artificial nucleic acid molecules
DK2972351T3 (da) Forbedrede analysemetoder
DK2970951T3 (da) Fremgangsmåder til nukleinsyresekventering
DK2890402T3 (da) Modificerede aminosyrer omfattende en azidogruppe
DK3351633T3 (da) Antisense-nukleinsyre
DK3138489T3 (da) Sæt til bestemmelse af en analytkoncentration
DK3014641T3 (da) Biokemisk energiomdannelsescelle
GB201419367D0 (en) Nucleic acid probe
BR112014028123A2 (pt) extração de ácidos nucleicos
BR112014024421A2 (pt) métodos de preparação de amostras para amplificação de ácido nucleico
BR112014029365A2 (pt) inibidores seletivos de células indiferenciadas
EP2915815A4 (en) NEW MODIFIED NUCLEIC ACID
DK2802660T3 (da) Nye C5A-bindende nucleinsyrer
EP3066193C0 (en) CELL CULTURE PROCESS