KR950701974A - 신규한 효소 제제 및 그의 제조방법(novel enzyme preparations and methods for their production) - Google Patents
신규한 효소 제제 및 그의 제조방법(novel enzyme preparations and methods for their production)Info
- Publication number
- KR950701974A KR950701974A KR1019940704357A KR19940704357A KR950701974A KR 950701974 A KR950701974 A KR 950701974A KR 1019940704357 A KR1019940704357 A KR 1019940704357A KR 19940704357 A KR19940704357 A KR 19940704357A KR 950701974 A KR950701974 A KR 950701974A
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- South Korea
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- nucleic acid
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- reesei
- acid sequence
- recombinant
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- 108090000790 Enzymes Proteins 0.000 title claims abstract 4
- 102000004190 Enzymes Human genes 0.000 title claims abstract 4
- 238000004519 manufacturing process Methods 0.000 title claims abstract 3
- 238000000034 method Methods 0.000 title claims 8
- 229940079919 digestives enzyme preparation Drugs 0.000 title abstract 3
- 229940088598 enzyme Drugs 0.000 title abstract 2
- 238000009472 formulation Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 101100429194 Hypocrea jecorina (strain QM6a) xyn2 gene Proteins 0.000 claims abstract description 9
- 241000499912 Trichoderma reesei Species 0.000 claims abstract description 9
- 101150052795 cbh-1 gene Proteins 0.000 claims description 3
- 239000013612 plasmid Substances 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 241000223259 Trichoderma Species 0.000 claims 6
- 239000001963 growth medium Substances 0.000 claims 5
- 229920002678 cellulose Polymers 0.000 claims 3
- 239000001913 cellulose Substances 0.000 claims 3
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims 2
- 101710098247 Exoglucanase 1 Proteins 0.000 claims 2
- 102000004317 Lyases Human genes 0.000 claims 2
- 108090000856 Lyases Proteins 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 101000899859 Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) Endoglucanase 1 Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 101710126559 Endoglucanase EG-II Proteins 0.000 claims 1
- 101710098246 Exoglucanase 2 Proteins 0.000 claims 1
- 101150114858 cbh2 gene Proteins 0.000 claims 1
- 230000000593 degrading effect Effects 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 101150066032 egl-1 gene Proteins 0.000 claims 1
- 101150003727 egl2 gene Proteins 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 230000001461 cytolytic effect Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 2
- 230000004988 N-glycosylation Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 150000002307 glutamic acids Chemical class 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Paper (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
T. reesei xln1과 xln2유전자의 구조 및 단백질의 일차 구조가 설명된다. 헤민셀룰라제 효소가 풍부한 효소 제제가 설명된다. 이러한 효소 제제들은 또한 부분적으로 또는 완전하게 셀룰로스 분해 활성이 결여될 수 있다. 이러한 제제들은 조질의 정제되지 않은 상태로 사용될 수 있으며 특히 펄프 및 종이 제조에 유됴하다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 유전자를 결실시키기 위한 일반적인 전략을 나타낸 도면.
제2도는 pAL475의 5.7kb(kpnl)삽입물의 제한효소 지도를 나탄 도면. xln2 유전자의 위치가 화살표로 표시되어 있다. 서브클론 pALK573, pALK574, pALK570 및 pALK476에의 삽입이 분리선으로 표시되어 있다. 단편의 5′ 말단에서의 Smal위치가 pUC19의 폴리링커로부터 유도된다.
제3도는 T. reesei xln2 유전자의 뉴클레오타이드 서열을 나타낸 도면〔SEQ ID No. :1:〕. 코딩 대역은 대문자로 표시되어 있다〔SEQ ID No. :2:〕. 정제된 단백질로부터 얻어진 펩티드 서열이 밑줄 표시되어 있으며; 이중 밑줄 그어진 서얼을 이용하여 PCR 프라이머를 제조하였다. TATA 박스는 도트로 표시하였다. 추정적인 시그날 분해 부위를 화살표(↑)로 표시하고 N-글리코실화의 추정 부위는 삼각형 (▼)으로 표시하였다. 성숙한 단백질의 N-말단에 화살표(→)표시를 하였다. 활성부위와 관련 있을 것으로 추측되는 두개의 글루탐산을 박스 안에 나타내었다.
제4도는 xln2 유전자를 cbh1위치에 표적화시키기 위한 플라스미드인 플라스미드 pALK476을 도시한다. xln2 유전자는 그 자신의 프로모터의 통제하에 있다.
제5도는 형질전환에 사용되는 pALK476단편을 도시한 것이다. 1.9kb cbh1 5'-플랭킹 대약(Scal-EcoRl)은 cbh1-코딩 대역의 2.2kb상류로 부터 시작되며, 1.8kb 3'-대역(BamHI-EcoRdI)은 cbh1-코딩 대역 말단의 1.4kb하류로부터 시작된다. amidS 유전자는 p3SR2(3.1 kb SpeI-XbaI 단편)로 부터, xln2 유전자는 pALK475(5.Okb Smal 단편, 제2도 참조)로 부터 유래하였다. 단편 중의 xln2 유전자의 프로모터 대역은 2.3kb 크기였다.
Claims (23)
- 트리코더마 리제이(T. reesei) pI 5.5자일라나제의 아미노산 서열 (SEQ ID No. 4)을 코딩하는 핵산 서열 또는 상기 분자에 하이브리다이즈되는 다른 핵산 분자로 이루어지는, 분리된 핵산 분자.
- 제1항에 있어서, 상기 핵산 서열이 SEQ ID No.3의 DNA 서열의 핵산 서열인 분리된 핵산 분자.
- 트리코더마 리제이(T. reesei) pI 9 자일라나제의 아미노산 서열(SEQ ID No. 2)을 코딩하는 핵산 서열 또는 상기 분자에 하이브리다이즈되는 다른 핵산 분자로 이루어지는, 분리된 핵산 분자.
- 제3항에 있어서, 상기 핵산 서열이 SEQ ID NO. 1의 DNA 서열의 핵산 서열인 분리된 핵산 분자.
- 제1항 내지 4항 중 어느 한 항에 따른 분리된 핵산 분자를 함유하는 재조합 벡터.
- 제5항에 있어서, 상기 벡터가 플라스미드 및 직쇄상 DNA 중에서 선택되는 재조합 벡터.
- 제5항에 따른 재조합 벡터에 의해 형질전환된 재조합 숙주.
- 제6항에 있어서, 상기 숙주가 트리코더마(Trichoderma)인 재조합 숙주
- 제8항에 있어서, 상기 트리코더마(Trichoderma)가 T.reesei인 재조합 숙주.
- 제1항 내지 4항 중 어느 한항에 따른 분리된 핵산 분자를 함유하는 재조합 벡터로서, 상기 핵산 서열이 xlnl 프로모터, xln2 프로모터, cbh1 프로모터, cbh2프로모터, egl1 프로모터, 및 egl2프로모터 중에서 선택된 T. reesei 프로모터에 작동적으로 연결된 것인 재조합 벡터.
- 제10항에 따른 재조합 벡터에 의해 형질전환된 재조합 숙주.
- 제11항에 있어서, 상기 숙주가 Trichoderma인 재조합 숙주.
- 제12항에 있어서, 상기 Trichoderma가 T. reesei인 재조합 숙주.
- (1)T. reesei xln1 및/또는 T. reesei xln2를 코딩하는 재조합 구축물로 숙주 세포를 형질전환시키고; (2)상기 (1)단계의 숙주 세포로부터 xln1및/또는 xln2가 발현되는 조건 하에서 상기 숙주세포를 배양한 다음; (3)상기 배양 배지를 수집하는 단계로 이루어지는, 자일라나제 활성이 풍부한 효소 제제의 제조방법.
- 제14항에 있어서, 상기 숙주 세포가 한가지 이상의 내인성 셀루로스 분해효소를 유전학적으로 발현할 수 없는 것인 방법.
- 제15항에 있어서, 상기 셀룰로스 분해효소가 CBHI, CBHII, EGI 및 EGII중에서 선택되는 방법.
- 제16항에 있어서, 상기 셀룰로스 분해효소가 CBHI인 방법.
- 제14항 내지 17항 중 어느 한 항에 있어서, 상기 숙주가 Trichoderma인 방법.
- 제18항에 있어서, 사익 TRichoderma가 T. reesei인 방법.
- 제7항 내지 9항 또는 11항 내지 13항 중 어느 한 항에 따른 숙주를 배양한 후 얻어진 배양 배지.
- 제20항에 따른 배양 배지를 정제, 건조, 농축 또는 고정화시킴으로써 유도된 생성물.
- 제20항에 따른 배양 배지 또는 제21항에 따른 유도된 생성물의 펄프 및 종이 산업에 있어서의 용도.
- 제20항에 따른 배양 배지 또는 제21항에 따른 유도된 생성물의 사료 산업에 있어서의 용도.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88989392A | 1992-05-29 | 1992-05-29 | |
| US889,893 | 1992-05-29 | ||
| PCT/FI1993/000221 WO1993024621A1 (en) | 1992-05-29 | 1993-05-24 | Novel enzyme preparations and methods for their production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR950701974A true KR950701974A (ko) | 1995-05-17 |
Family
ID=25395958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019940704357A Withdrawn KR950701974A (ko) | 1992-05-29 | 1993-05-24 | 신규한 효소 제제 및 그의 제조방법(novel enzyme preparations and methods for their production) |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0670893A1 (ko) |
| JP (1) | JPH08500727A (ko) |
| KR (1) | KR950701974A (ko) |
| AU (1) | AU4071893A (ko) |
| BR (1) | BR9306451A (ko) |
| CA (1) | CA2136350C (ko) |
| WO (1) | WO1993024621A1 (ko) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7816129B2 (en) | 1994-07-29 | 2010-10-19 | Ab Enzymes Gmbh | Production and secretion of proteins of bacterial origin in filamentous fungi |
| CN1165614C (zh) * | 1995-01-26 | 2004-09-08 | 诺沃奇梅兹有限公司 | 含有木聚糖酶的动物饲料添加剂 |
| US6723549B2 (en) | 1995-10-17 | 2004-04-20 | Ab Enzymes Oy | Cellulases, the genes encoding them and uses thereof |
| CA2232245C (en) | 1995-10-17 | 2011-06-07 | Rohm Enzyme Finland Oy | Cellulases, the genes encoding them and uses thereof |
| US6184019B1 (en) | 1995-10-17 | 2001-02-06 | Röhm Enzyme Finland OY | Cellulases, the genes encoding them and uses thereof |
| US6228629B1 (en) | 1995-12-18 | 2001-05-08 | Röhn Enzyme Finland OY | Xylanases, genes encoding them, and uses thereof |
| US6635464B1 (en) | 1995-12-18 | 2003-10-21 | Rohm Enzyme Finland Oy | Xylanases, genes encoding them, and uses thereof |
| EP0868506A1 (en) * | 1995-12-18 | 1998-10-07 | Röhm Enzyme Finland Oy | Novel xylanases and uses thereof |
| EP1433843A3 (en) * | 1995-12-18 | 2005-04-13 | AB Enzymes Oy | Novel xylanases, genes encoding them, and uses thereof |
| US6001595A (en) * | 1996-11-29 | 1999-12-14 | Rohm Enzyme GmbH | Promoters and uses thereof |
| US6015703A (en) * | 1998-03-10 | 2000-01-18 | Iogen Corporation | Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase |
| JP4927281B2 (ja) * | 1999-11-30 | 2012-05-09 | ノボザイムス，インコーポレイティド | 共通翻訳開始配列を用いるポリペプチドの産生方法 |
| WO2006016596A1 (ja) * | 2004-08-12 | 2006-02-16 | Oji Paper Co., Ltd. | リグノセルロース材料からの繊維成分の製造およびその利用 |
| DE102004050410A1 (de) | 2004-10-15 | 2006-06-08 | Ab Enzymes Gmbh | Polypeptid mit Phytaseaktivität und dieses codierende Nucleotidsequenz |
| DE102006053059A1 (de) | 2006-11-10 | 2008-05-15 | Ab Enzymes Gmbh | Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz |
| US10167576B2 (en) | 2012-05-21 | 2019-01-01 | Oji Holdings Corporation | Method of producing fine fiber, and fine fiber, non-woven fabric, and fine fibrous cellulose |
| US20200002695A1 (en) * | 2017-02-23 | 2020-01-02 | Toray Industries, Inc. | Xylanase variant and enzyme composition for decomposing biomass |
| EP3728578A1 (en) | 2017-12-20 | 2020-10-28 | DSM IP Assets B.V. | Animal feed compositions and uses thereof |
| AU2019341519A1 (en) | 2018-09-17 | 2021-03-18 | Dsm Ip Assets B.V. | Animal feed compositions and uses thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI904214A7 (fi) * | 1990-08-27 | 1992-02-28 | Valtion Teknillinen Tutkimuskeskus | Hemiselluloosan hydrolysointimenetelmä |
| DK0553280T4 (da) * | 1990-10-05 | 2010-11-15 | Genencor Int | Fremgangsmåde til behandling af bomuldsholdige tekstiler med cellulase |
-
1993
- 1993-05-24 AU AU40718/93A patent/AU4071893A/en not_active Abandoned
- 1993-05-24 JP JP6500232A patent/JPH08500727A/ja active Pending
- 1993-05-24 EP EP93910050A patent/EP0670893A1/en not_active Ceased
- 1993-05-24 CA CA002136350A patent/CA2136350C/en not_active Expired - Lifetime
- 1993-05-24 WO PCT/FI1993/000221 patent/WO1993024621A1/en not_active Application Discontinuation
- 1993-05-24 KR KR1019940704357A patent/KR950701974A/ko not_active Withdrawn
- 1993-05-24 BR BR9306451A patent/BR9306451A/pt not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2136350A1 (en) | 1993-12-09 |
| CA2136350C (en) | 2009-04-07 |
| JPH08500727A (ja) | 1996-01-30 |
| EP0670893A1 (en) | 1995-09-13 |
| AU4071893A (en) | 1993-12-30 |
| BR9306451A (pt) | 1998-06-30 |
| WO1993024621A1 (en) | 1993-12-09 |
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