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KR950032281A - 혈장 헤파린 결합 단백질-70 - Google Patents

혈장 헤파린 결합 단백질-70 Download PDF

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Publication number
KR950032281A
KR950032281A KR1019950006971A KR19950006971A KR950032281A KR 950032281 A KR950032281 A KR 950032281A KR 1019950006971 A KR1019950006971 A KR 1019950006971A KR 19950006971 A KR19950006971 A KR 19950006971A KR 950032281 A KR950032281 A KR 950032281A
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Prior art keywords
heparin binding
binding protein
plasma heparin
protein
plasma
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KR1019950006971A
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KR100371976B1 (ko
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마사요시 고야마
미키코 다카하시
가즈유키 도이
Original Assignee
사토 미쓰아키
훽스트 저팬 리미티드
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Publication of KR950032281A publication Critical patent/KR950032281A/ko
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Publication of KR100371976B1 publication Critical patent/KR100371976B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

상처 치유 활성이 있는 혈장 헤파린 결합 단백질(PHBP)-70으로 명명된 단백질은 환원 조건하에서 SDS-PAGE를 이용한 분석에 의해 측정된 분자량이 약 60 내지 80KDa이며 사람을 포유동물의 태아 및 신생아의 혈액으로부터 수득될 수 있다.
단백질 PHBP-70은 섬유아세포 증식 활성을 지닌 신규단백질이므로 상처 치유제로서 유용하다.

Description

혈정 헤파린 결합 단백질-70
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 역상 액체 크로마토그래피에 의해 전개된 분획의 용출 양식을 도시한 것이다. 이 분획은 헤파린 친화 컬럼 크로카토그래피에 의해 수득된다. 화살표는 단백질을 함유하는 활성 분획을 나타낸다. 제2도는 분광광도계를 사용하여 280nm에서의 흡광도를 기록한 세파크릴 S-200HR 겔 여과의 용출 양식을 도시한 것이다. 제3도는 분광광도계를 사용하여 280nm에서의 흡광도를 기록한 모노 Q 칼럼의 용출 양식을 도시한 것이다. 용출은 0.1 내지 1.0M 염화나트륨의 선형 구배로 수행한다. 제4도는 분광광도계를 사용하여 214nm에서의 흡광도를 기록한 5C18-300의 용출 양식을 도시한 것이다. 용출은 10 내지 70%아세토니트릴의 선형 구배로 수행한다. 제5도는 피크 1에서 펩타이드의 전기영동 양식을 도시한 것이다.

Claims (4)

  1. 환원 조건하에서 SDS-PAGE를 이용한 분석에 의해 측정한 분자량이 약 60 내지 80KDa이고 서열 리스트의 서열 확인 번호: 1에 기술된 N-말단 아미노산 서열을 지니는, 혈장 헤파린 결합 단백질-70.
  2. 제1항에 있어서, 포유동물 혈액으로부터 기원한 혈장 헤파린 결합 단백질-70.
  3. 제1항 또는 제2항에 있어서, 헤파린 결합 성장인자인 혈장 헤파린 결합 단백질-70.
  4. 제1항 내지 제3항중 어느 한 항에 따른 혈장 헤파린 결합 단백질-70을 포함하는 상처 치유제.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950006971A 1994-03-31 1995-03-30 혈장헤파린결합단백질-70 KR100371976B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP6061905A JPH07267992A (ja) 1994-03-31 1994-03-31 新規なタンパク質ｐｈｂｐ−７０
JP94-061905 1994-03-31

Publications (2)

Publication Number Publication Date
KR950032281A true KR950032281A (ko) 1995-12-20
KR100371976B1 KR100371976B1 (ko) 2003-03-31

Family

ID=13184646

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950006971A KR100371976B1 (ko) 1994-03-31 1995-03-30 혈장헤파린결합단백질-70

Country Status (6)

Country Link
US (1) US5545720A (ko)
EP (1) EP0675135A3 (ko)
JP (1) JPH07267992A (ko)
KR (1) KR100371976B1 (ko)
AU (1) AU689852B2 (ko)
CA (1) CA2145960A1 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6670198A (en) * 1997-02-28 1998-09-18 The Texas A & M University System Heparan sulfate/heparin interacting protein compositions and methods of use
US20050142208A1 (en) * 2002-05-09 2005-06-30 Won Min Yoo Pharmceutical composition for treatment of wounds conntaining blood plasma or serum
KR20070113876A (ko) * 2006-05-26 2007-11-29 메디제네스(주) 혈장성분을 함유하는 창상 치료용 약학 조성물

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5166317A (en) * 1988-10-31 1992-11-24 Houston Biotechnology Incorporated Neurotrophic factor
US5227302A (en) * 1988-12-20 1993-07-13 Ludwig Institute For Cancer Research DNA encoding platelet derived endothelial cell growth factor (PD-ECGF)
EP0488196A3 (en) * 1990-11-30 1993-04-07 Takeda Chemical Industries, Ltd. Hst-2, a member of the heparin binding growth factor family

Also Published As

Publication number Publication date
EP0675135A3 (en) 1996-02-07
US5545720A (en) 1996-08-13
JPH07267992A (ja) 1995-10-17
KR100371976B1 (ko) 2003-03-31
AU689852B2 (en) 1998-04-09
EP0675135A2 (en) 1995-10-04
CA2145960A1 (en) 1995-10-01
AU1617495A (en) 1995-10-12

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