KR930019696A - 탄수화물의 생산 방법 - Google Patents
탄수화물의 생산 방법 Download PDFInfo
- Publication number
- KR930019696A KR930019696A KR1019930003656A KR930003656A KR930019696A KR 930019696 A KR930019696 A KR 930019696A KR 1019930003656 A KR1019930003656 A KR 1019930003656A KR 930003656 A KR930003656 A KR 930003656A KR 930019696 A KR930019696 A KR 930019696A
- Authority
- KR
- South Korea
- Prior art keywords
- oligosaccharides
- peptide
- peptides
- present
- produced
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 150000001720 carbohydrates Chemical class 0.000 title 1
- 235000014633 carbohydrates Nutrition 0.000 title 1
- 229920001542 oligosaccharide Polymers 0.000 claims abstract 9
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract 9
- 239000004480 active ingredient Substances 0.000 claims 1
- 230000003110 anti-inflammatory effect Effects 0.000 claims 1
- 239000003085 diluting agent Substances 0.000 claims 1
- 239000003937 drug carrier Substances 0.000 claims 1
- 239000000546 pharmaceutical excipient Substances 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 7
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 230000021164 cell adhesion Effects 0.000 abstract 2
- 206010061218 Inflammation Diseases 0.000 abstract 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 abstract 1
- OVRNDRQMDRJTHS-KEWYIRBNSA-N N-acetyl-D-galactosamine Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O OVRNDRQMDRJTHS-KEWYIRBNSA-N 0.000 abstract 1
- MBLBDJOUHNCFQT-UHFFFAOYSA-N N-acetyl-D-galactosamine Natural products CC(=O)NC(C=O)C(O)C(O)C(O)CO MBLBDJOUHNCFQT-UHFFFAOYSA-N 0.000 abstract 1
- OVRNDRQMDRJTHS-RTRLPJTCSA-N N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-RTRLPJTCSA-N 0.000 abstract 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 abstract 1
- 102000019997 adhesion receptor Human genes 0.000 abstract 1
- 108010013985 adhesion receptor Proteins 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000004054 inflammatory process Effects 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 101500025568 Homo sapiens Saposin-D Proteins 0.000 description 1
- 229940100689 human protein c Drugs 0.000 description 1
- 150000004677 hydrates Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6464—Protein C (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Rheumatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pain & Pain Management (AREA)
- Epidemiology (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Compounds Of Unknown Constitution (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Amplifiers (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
Abstract
본 발명은 펩타이드의 발현에 적당한 조헌하에서 글리코실화될 수 있는 펩타이드를 발현하는 인간 신장 293세포를 배양한 다음, 세포 또는 세포 배양물 상층액으로부터 펩타이드를 회수함으로서 서열 GalNAc (1-4) (Fuc(1-3)) GlcNAc를 가진 올리고당을 포함하는 펩타이드의 생산 방법에 관한 것이다.
본 발명은 또한 293 세포에서 생산된 펩타이드로부터 신규 올리고당을 더 단리하기 위한 방법을 포함한다. 생산된 펩타이드 및/또는 올리고당은 특정 세포 유착 수용체의 고 친화성 리간드로서 작용 하도록 사용될 수 있다. 펩타이드 및/또는 올리고당은 세포유착 및 염증을 방지하거나 감소시키기 위해 사용될 수 있다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 293 세포에서 생산된 인간 단백질 C 분자상에서 발견된 주요 난수화물들의 크로마토그래프를 나타낸 것이다.
Claims (5)
- 하기 구조의 올리고당.
- 하기 구조의 올리고당.
- 하기 구조의 올리고당.
- 하기 구조의 올리고당.
- 하나이상의 약제학적으로 허용되는 담체, 부형제 또는 희석제와 함께 제1항, 2항, 3항 또는 4항 중 어느 한 항의 올리고당을 활성성분으로서 함유하는 소염제 조성물.※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US84986892A | 1992-03-12 | 1992-03-12 | |
| US849,868 | 1992-03-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR930019696A true KR930019696A (ko) | 1993-10-18 |
Family
ID=25306715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019930003656A Withdrawn KR930019696A (ko) | 1992-03-12 | 1993-03-11 | 탄수화물의 생산 방법 |
Country Status (23)
| Country | Link |
|---|---|
| EP (1) | EP0565241B1 (ko) |
| JP (1) | JP3171506B2 (ko) |
| KR (1) | KR930019696A (ko) |
| CN (1) | CN1079967A (ko) |
| AT (1) | ATE137241T1 (ko) |
| AU (1) | AU661762B2 (ko) |
| BR (1) | BR9301136A (ko) |
| CA (1) | CA2091521A1 (ko) |
| CY (1) | CY1947A (ko) |
| CZ (1) | CZ39093A3 (ko) |
| DE (1) | DE69302317T2 (ko) |
| DK (1) | DK0565241T3 (ko) |
| ES (1) | ES2088227T3 (ko) |
| FI (1) | FI931086L (ko) |
| GR (1) | GR3020406T3 (ko) |
| HK (1) | HK132396A (ko) |
| HU (1) | HUT69613A (ko) |
| IL (1) | IL105018A0 (ko) |
| MX (1) | MX9301344A (ko) |
| NO (1) | NO930890L (ko) |
| NZ (1) | NZ247105A (ko) |
| YU (1) | YU16993A (ko) |
| ZA (1) | ZA931723B (ko) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1384786B1 (en) * | 2001-04-02 | 2010-01-27 | Glycomedics, Inc. | Process for producing oligosaccharide chains |
| FI20011664L (fi) * | 2001-08-17 | 2003-02-18 | Carbion Oy | Syöpäpesifiset oligoskkaridisekvenssit ja niiden käyttö |
| US7943763B2 (en) * | 2002-07-05 | 2011-05-17 | Otsuka Chemical Holdings Co., Ltd. | Process for preparing glycopeptides having asparagine-linked oligosaccharides, and the glycopeptides |
| CN100378128C (zh) * | 2002-12-26 | 2008-04-02 | 大塚化学株式会社 | 3支链型糖链天冬酰胺衍生物、该糖链天冬酰胺、该糖链和它们的制造方法 |
| JP3884725B2 (ja) | 2003-06-03 | 2007-02-21 | 三菱電機株式会社 | 導波管装置 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5009889A (en) * | 1987-12-31 | 1991-04-23 | Oklahoma Medical Research Foundation | Treatment of dysfunctional vascular endothelium using activated protein C |
-
1993
- 1993-03-10 NZ NZ247105A patent/NZ247105A/en unknown
- 1993-03-10 CZ CZ93390A patent/CZ39093A3/cs unknown
- 1993-03-10 HU HU9300687A patent/HUT69613A/hu unknown
- 1993-03-10 ZA ZA931723A patent/ZA931723B/xx unknown
- 1993-03-11 KR KR1019930003656A patent/KR930019696A/ko not_active Withdrawn
- 1993-03-11 YU YU16993A patent/YU16993A/sh unknown
- 1993-03-11 AU AU35199/93A patent/AU661762B2/en not_active Ceased
- 1993-03-11 CA CA002091521A patent/CA2091521A1/en not_active Abandoned
- 1993-03-11 FI FI931086A patent/FI931086L/fi not_active Application Discontinuation
- 1993-03-11 BR BR9301136A patent/BR9301136A/pt not_active Application Discontinuation
- 1993-03-11 MX MX9301344A patent/MX9301344A/es not_active Application Discontinuation
- 1993-03-11 IL IL105018A patent/IL105018A0/xx unknown
- 1993-03-11 JP JP05056193A patent/JP3171506B2/ja not_active Expired - Fee Related
- 1993-03-11 NO NO93930890A patent/NO930890L/no unknown
- 1993-03-11 CN CN93104040A patent/CN1079967A/zh active Pending
- 1993-03-12 CY CY194793A patent/CY1947A/xx unknown
- 1993-03-12 EP EP93301878A patent/EP0565241B1/en not_active Expired - Lifetime
- 1993-03-12 DE DE69302317T patent/DE69302317T2/de not_active Expired - Fee Related
- 1993-03-12 AT AT93301878T patent/ATE137241T1/de not_active IP Right Cessation
- 1993-03-12 DK DK93301878.0T patent/DK0565241T3/da active
- 1993-03-12 ES ES93301878T patent/ES2088227T3/es not_active Expired - Lifetime
-
1996
- 1996-06-12 GR GR960401581T patent/GR3020406T3/el unknown
- 1996-07-18 HK HK132396A patent/HK132396A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI931086L (fi) | 1993-09-13 |
| DK0565241T3 (da) | 1996-05-28 |
| NZ247105A (en) | 1994-11-25 |
| EP0565241B1 (en) | 1996-04-24 |
| DE69302317D1 (de) | 1996-05-30 |
| ES2088227T3 (es) | 1996-08-01 |
| HU9300687D0 (en) | 1993-05-28 |
| EP0565241A1 (en) | 1993-10-13 |
| AU3519993A (en) | 1993-09-16 |
| IL105018A0 (en) | 1993-07-08 |
| MX9301344A (es) | 1993-09-01 |
| CZ39093A3 (en) | 1994-03-16 |
| ZA931723B (en) | 1994-09-10 |
| CY1947A (en) | 1993-03-12 |
| BR9301136A (pt) | 1993-09-28 |
| AU661762B2 (en) | 1995-08-03 |
| JP3171506B2 (ja) | 2001-05-28 |
| DE69302317T2 (de) | 1996-09-19 |
| YU16993A (sh) | 1996-02-19 |
| NO930890L (no) | 1993-09-13 |
| CA2091521A1 (en) | 1993-09-13 |
| GR3020406T3 (en) | 1996-09-30 |
| HK132396A (en) | 1996-07-26 |
| JPH0625302A (ja) | 1994-02-01 |
| ATE137241T1 (de) | 1996-05-15 |
| NO930890D0 (no) | 1993-03-11 |
| FI931086A0 (fi) | 1993-03-11 |
| HUT69613A (en) | 1995-09-28 |
| CN1079967A (zh) | 1993-12-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 19930311 |
|
| PG1501 | Laying open of application | ||
| PC1203 | Withdrawal of no request for examination | ||
| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |