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KR920019931A - 핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법 - Google Patents

핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법 Download PDF

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KR920019931A
KR920019931A KR1019920005492A KR920005492A KR920019931A KR 920019931 A KR920019931 A KR 920019931A KR 1019920005492 A KR1019920005492 A KR 1019920005492A KR 920005492 A KR920005492 A KR 920005492A KR 920019931 A KR920019931 A KR 920019931A
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nucleic acids
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브로치 윌
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더 퍼킨 엘머 코포레이션
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Abstract

내용 없음

Description

핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (19)

  1. (a) (1) 디알킬암모늄, 트리알킬암모늄 및 테트라알킬암모늄(여기에서, 알킬 그룹은 메틸 및 에틸의 배합으로 이루어진다)으로 이루어진 그룹 중에서 선택된 양이온과 (2) 브로마이드, 클로라이드, 아세테이트, 포르메이트, 니트레이트, 피클로레이트, 디하이드로겐 포스페이트, 에탄 설포네이트 및 메탄 설포네이트로 이루어진 그룹 중에서 선택된 음이온의 동일 농도로 구성된 0.5 내지 1.5M의 적당한 농도 범위의 용출염 및 (b) 약 0.05M의 농도를 초과하지 않고 3.5 내지 8.5의 적당한 pKa 범위를 갖는 완충산을 함유하고, 약 4 내지 8의 pH 범위를 가지며, 물에 대하여 260㎚(광로 1㎝)에서의 흡광도가 약 0.1을 초과하지 않는, 핵산의 음이온-교환 크로마토그래피에 유용한 수성용매.
  2. 제 1항에 있어서, 완충산이 양이온이고 양이온이거나 중성인 하전을 갖는 짝염기를 갖는 용매.
  3. 수중 조성물의 1/2 내지 1/20 희석 결과 제 1항의 용매를 제공하도록 제 1항에 따른 용매의 약 2배 내지 20배 농축물을 함유하는 수성 조성물.
  4. 제 1항에 있어서, 약 1㎍/L 내지 1g/L의 농도 범위로 핵산을 추가로 함유하는 조성물.
  5. 제 4항에 있어서, 핵산이 이본쇄 DNA인 조성물.
  6. 제 1항의 용매가 음이온-교환 고체와 배합된 조성물.
  7. 제 6항에 있어서, 고체가 폴리크릴계 골격을 포함하는 조성물.
  8. 제 6항에 있어서, 고체가 디에틸아미노에틸 작용그룹을 포함하는 조성물.
  9. 제 6항에 있어서, 고체가 폴리에틸렌이민 작용그룹을 포함하는 조성물.
  10. 제 6항에 있어서, 고체가 약 10㎛ 내지 2㎛의 평균 직경을 입자를 포함하는 조성물.
  11. 제 6항에 있어서, 고체가 실질적으로 비공성인 조성물.
  12. 제 6항에 있어서, 고체가 폴리스티렌 골격을 포함하는 조성물.
  13. 제 6항에 있어서, 약 1㎍/L 내지 1g/L의 농도 범위의 핵산을 추가로 함유하는 조성물.
  14. (a) 핵산을 함유하는 시험샘플을 음이온-교환고체와 접촉시키고, (b) 단계 (a)의 고체를 일련의 제 1항의 용매(여기에서, 용출염의 농도는 용매의 대부분이 접촉 후 고체로부터 분리되도록 약 0.5M로부터 약 1.5M로 체계적으로 증가된다)와 접촉시키며, (c) 핵산에 대해 일련의 용매를 분석함을 특징으로 하여, 분자 크기의 차이를 근거로 핵산을 분리하고 분석하는 방법.
  15. 제 14항에 있어서, 고체와 접촉하는 용출염의 농도가 연속적으로 증가하는 방법.
  16. 제 14항에 있어서, 고체로부터 분리된 용매 중의 핵산의 농도를 약 250㎚ 내지 290㎚의 파장 범위에서 자외선 흡광도로 측정하는 방법.
  17. 제 14항에 있어서, 고체가 약 10㎛ 내지 약 2㎛의 평균 직경을 갖는 입자를 포함하는 방법.
  18. 제 17항에 있어서, 고체가 직경이 약 1㎜ 내지 약 6㎜이고 길이가 약 10㎜ 내지 약 60㎜인 실린더형 컬럼에 함유된 방법.
  19. 제 14항에 있어서, 공정을 완성하는데 필요한 총 시간이 약 2분 내지 약 30분인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920005492A 1991-04-03 1992-04-02 핵산의 음이온 교환 크로마토그래피에 유용한 수성 용매, 이를 함유하는 수성 조성물 및 이를 사용한 핵산의 개선된 분리 방법 KR920019931A (ko)

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US67973691A 1991-04-03 1991-04-03
US7/679,736 1991-04-03

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AT (2) ATE220341T1 (ko)
AU (1) AU645674B2 (ko)
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DE (3) DE69232677T2 (ko)
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EP0691148A3 (ko) 1996-01-24
AU1403492A (en) 1992-10-08
ATE220341T1 (de) 2002-07-15
IL101356A0 (en) 1992-11-15
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ATE154132T1 (de) 1997-06-15
EP0507591A3 (en) 1993-02-03
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EP0691148B1 (en) 2002-07-10
IL101356A (en) 1996-08-04
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CA2063855A1 (en) 1992-10-04
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AU645674B2 (en) 1994-01-20
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