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KR910018551A - 스트렙토마이세스에서의 외래 단백질의 제조방법 - Google Patents

스트렙토마이세스에서의 외래 단백질의 제조방법 Download PDF

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Publication number
KR910018551A
KR910018551A KR1019910006250A KR910006250A KR910018551A KR 910018551 A KR910018551 A KR 910018551A KR 1019910006250 A KR1019910006250 A KR 1019910006250A KR 910006250 A KR910006250 A KR 910006250A KR 910018551 A KR910018551 A KR 910018551A
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South Korea
Prior art keywords
gene
protein
fusion protein
interest
gene construct
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KR0168669B1 (ko
Inventor
콜러 클라우스-페터
리쓰 권터
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펜스케, 라피체
훽스트 아크티엔게젤샤프트
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음

Description

스트렙토마이세스에서의 외래 단백질의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 목적하는 단백질에 대한 구조 유전자를 텐다미스타트(tendamistat)의 시그날 서열 및 대략 처음 10개의 아미노-말단 아미노산에 대한 코돈(여기에서, 덴다미스타트 유전자 서열은 변화시킬 수 있다)에 커플링시키고, 이 유전자 구조물을 스크랩토마이세스 숙주 세포에서 발현시킨 다음, 분비된 융합 단백질을 상등액으로부터 분리 시킴을 특징으로 하는, 융합 단백질의 제조방법.
  2. 텐다미스타트의 시그날 서열 및 대략 처음 10개의 코돈 및 다른 단백질에 대한 구조 유전자를 함유하는 유전자 구조물.
  3. 제2항에 있어서, 아미노산에 대한 코돈이 덴다미스타트 영역에서 결실되거나, 대체되거나 또는 부가된 유전자 구조물.
  4. 제2항 또는 제3항에 있어서, 링커 서열이 덴다미스타트 유전자와 목적하는 단백질의 구조 유전자 사이에 배열되어 있는 유전자 구조물.
  5. 제2항, 제3항 또는 제4항에서 청구된 유전자 구조물을 함유하는 벡터.
  6. 제5항에서 청구된 벡터를 함유하는 스트렙토마이세스 세포.
  7. 텐다미스타트의 대략 처음 10개의 아미노산의 N-말단부분이, 필요한 경우 브릿지 서열을 통해, 목적하는 단백질에 커플링된 융합 단백질.
  8. 목적하는 단백질을 제조하기 위한, 제7항에서 청구된 융합 단백질 또는 제1항에서 청구된 바와 같이 수득할 수 있는 융합 단백질의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910006250A 1990-04-21 1991-04-19 스트렙토마이세스에서의 외래 단백질의 제조방법 Expired - Lifetime KR0168669B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE?P4012818.0? 1990-04-21
DE4012818A DE4012818A1 (de) 1990-04-21 1990-04-21 Verfahren zur herstellung von fremdproteinen in streptomyceten
DEP4012818,0 1990-04-21

Publications (2)

Publication Number Publication Date
KR910018551A true KR910018551A (ko) 1991-11-30
KR0168669B1 KR0168669B1 (ko) 1999-01-15

Family

ID=6404857

Family Applications (1)

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KR1019910006250A Expired - Lifetime KR0168669B1 (ko) 1990-04-21 1991-04-19 스트렙토마이세스에서의 외래 단백질의 제조방법

Country Status (29)

Country Link
EP (1) EP0453969B1 (ko)
JP (1) JP3319605B2 (ko)
KR (1) KR0168669B1 (ko)
CN (1) CN1049248C (ko)
AT (1) ATE142263T1 (ko)
AU (1) AU630287B2 (ko)
BR (1) BR9101587A (ko)
CA (1) CA2040810C (ko)
CZ (1) CZ285440B6 (ko)
DE (2) DE4012818A1 (ko)
DK (1) DK0453969T3 (ko)
ES (1) ES2093043T3 (ko)
FI (1) FI911882L (ko)
GR (1) GR3021040T3 (ko)
HR (1) HRP940770A2 (ko)
HU (1) HU210358B (ko)
IE (1) IE911322A1 (ko)
IL (1) IL97903A0 (ko)
LT (1) LT3686B (ko)
LV (1) LV10494B (ko)
NO (1) NO911557L (ko)
NZ (1) NZ237882A (ko)
PL (2) PL169596B1 (ko)
PT (1) PT97427B (ko)
RU (1) RU2055892C1 (ko)
SK (1) SK110191A3 (ko)
TW (1) TW213487B (ko)
YU (1) YU48435B (ko)
ZA (1) ZA912937B (ko)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SK279686B6 (sk) * 1990-09-05 1999-02-11 Hoechst Aktiengesellschaft Spôsob hydrolýzy reťazca aminokyselín preproinzulí
EP0600372B1 (de) 1992-12-02 1997-02-05 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
ES2161726T3 (es) * 1993-04-27 2001-12-16 Hoechst Ag Formas amorfas monoesfericas de derivados de insulina.
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
CN1061375C (zh) * 1996-07-19 2001-01-31 中国科学院上海生物工程研究中心 利用异源启动子在链霉菌中表达透明颤菌血红蛋白
DE59711533D1 (de) 1996-07-26 2004-05-27 Aventis Pharma Gmbh Insulinderivate mit erhöhter Zinkbindung
DE19825447A1 (de) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh Neue Insulinanaloga mit erhöhter Zinkbildung
CN1298742C (zh) * 2003-06-03 2007-02-07 上海新药研究开发中心 一种适合于高效表达的融合蛋白及其生产方法
RU2395296C1 (ru) * 2009-02-19 2010-07-27 Общество С Ограниченной Ответственностью "Концерн О3" Способ получения препарата проинсулина для перорального применения
CN104818291A (zh) * 2015-05-08 2015-08-05 江南大学 一种链霉菌重组表达载体的构建及应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
DE3418274A1 (de) 1984-05-17 1985-11-21 Hoechst Ag, 6230 Frankfurt Signalpeptid fuer die exkretion von peptiden in streptomyceten
DE3707150A1 (de) * 1987-03-06 1988-09-15 Hoechst Ag Tendamistat-derivate
DE3714866A1 (de) * 1987-05-05 1988-11-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
DE3715033A1 (de) 1987-05-06 1988-11-17 Hoechst Ag Verfahren zur isolierung von fusionsproteinen
DE3716722A1 (de) 1987-05-19 1988-12-01 Hoechst Ag Gentechnologisches verfahren zur herstellung von angiogeninen
DE3843713A1 (de) * 1988-04-25 1989-11-02 Henkel Kgaa Verwendung von calcinierten hydrotalciten als katalysatoren fuer die ethoxylierung bzw. propoxylierung
ES2081826T3 (es) * 1988-11-03 1996-03-16 Hoechst Ag Procedimiento para la preparacion de un producto previo de insulina en estreptomicetos.

Also Published As

Publication number Publication date
YU48435B (sh) 1998-07-10
HUT57268A (en) 1991-11-28
TW213487B (ko) 1993-09-21
CA2040810A1 (en) 1991-10-22
NZ237882A (en) 1993-12-23
CN1055952A (zh) 1991-11-06
AU7515491A (en) 1991-10-24
DE4012818A1 (de) 1991-10-24
LV10494B (en) 1996-02-20
ES2093043T3 (es) 1996-12-16
PL169178B1 (pl) 1996-06-28
HRP940770A2 (en) 1997-06-30
YU69691A (sh) 1995-12-04
FI911882L (fi) 1991-10-22
DE59108128D1 (de) 1996-10-10
SK110191A3 (en) 1995-07-11
CZ110191A3 (en) 1993-08-11
DK0453969T3 (ko) 1997-02-10
ATE142263T1 (de) 1996-09-15
HU911302D0 (en) 1991-10-28
IE911322A1 (en) 1991-10-23
KR0168669B1 (ko) 1999-01-15
LT3686B (en) 1996-01-25
CZ285440B6 (cs) 1999-08-11
LTIP1523A (en) 1995-06-26
NO911557D0 (no) 1991-04-19
NO911557L (no) 1991-10-22
EP0453969A1 (de) 1991-10-30
LV10494A (lv) 1995-02-20
IL97903A0 (en) 1992-06-21
PL169596B1 (pl) 1996-08-30
ZA912937B (en) 1991-12-24
CN1049248C (zh) 2000-02-09
AU630287B2 (en) 1992-10-22
PL289953A1 (en) 1991-11-04
EP0453969B1 (de) 1996-09-04
RU2055892C1 (ru) 1996-03-10
BR9101587A (pt) 1991-12-10
GR3021040T3 (en) 1996-12-31
PT97427B (pt) 1998-08-31
JPH04228086A (ja) 1992-08-18
HU210358B (en) 1995-04-28
FI911882A0 (fi) 1991-04-18
JP3319605B2 (ja) 2002-09-03
CA2040810C (en) 2001-07-24
PT97427A (pt) 1992-01-31

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