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KR900001859A - Ldh₁의 분석법 - Google Patents

Ldh₁의 분석법 Download PDF

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Publication number
KR900001859A
KR900001859A KR1019890010104A KR890010104A KR900001859A KR 900001859 A KR900001859 A KR 900001859A KR 1019890010104 A KR1019890010104 A KR 1019890010104A KR 890010104 A KR890010104 A KR 890010104A KR 900001859 A KR900001859 A KR 900001859A
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South Korea
Prior art keywords
ldh
protease
sample
acid
isozyme
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KR1019890010104A
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English (en)
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KR910005633B1 (ko
Inventor
야스시 시라하세
겐지 이스시끼
요시후미 와따즈
Original Assignee
나까모또 히로유끼
곡사이사야꾸 가부시끼가이샤
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Publication of KR900001859A publication Critical patent/KR900001859A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

내용 없음.

Description

LDH1의 분석법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 시료중의 LDH2,LDH3,LDH4, 및 LDH5를 단백질 변성제 존재하에 프로테아제로 억제한 다음 억제되지 않고 남아있는 LDH1를 정량함을 특징으로 하는 시료중의 LDH1의 분별 정량법.
  2. 제1항에 있어서, 시료가 혈청 또는 혈장의 임상시료인 방법.
  3. 제1항에 있어서, 프로테아제가 α-키모트립신, 트립신, 트롬빈, 엘라스타아제, 엔도프로테아제, 서브틸리신, 브로멜라인, 파파인, 카르복시펩티다아제, 프로나아제, 펩신 및 카텝신으로 구성된 군으로부터 선택되는 방법.
  4. 제3항에 있어서, 프로테아제로서 α-키모트립신이 10∼1.000단위/ml의 농도 범위로 사용되는 방법.
  5. 제1항에 있어서, 단백질 변성제가 콜산, 데옥시콜산, 타우로콜산, 타우로데옥시콜산, 구아니딘(히드로클로라이드), 트리클로로아세트산, 우레아, 티오우레아 및 티오시아네이트로 구성된 군으로부터 최소한 하나가 선택되는 방법.
  6. 제5항에 있어서, 단백질 변성제로서 0.05∼5M 구아니딘이 사용되는 방법.
  7. 제1항에 있어서, 아이소자임과 색소 또는 염료 전구물질을 촉매적으로 현색한 후 가시광선 범위에서 흡광도를 측정하거나, 아이소자임의 촉매 효과에 의해 환원된 보효소 NADH의 자외부 흡광도를 측정함을 특징으로 하는 효소 분석법에 따라서 억제되지 않고 남아 있는 LDH1아이소자임이 정량되는 방법.
  8. 제7항에 있어서, pH조정없이 중성 조건하에서 분석이 수행되는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890010104A 1988-07-15 1989-07-15 Ldh₁의 분석법 KR910005633B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP63177579A JPH0775556B2 (ja) 1988-07-15 1988-07-15 Ｌｄｈアイソザイムのｌｄｈ▲下１▼の分別定量法
JP177579 1988-07-15

Publications (2)

Publication Number Publication Date
KR900001859A true KR900001859A (ko) 1990-02-27
KR910005633B1 KR910005633B1 (ko) 1991-08-01

Family

ID=16033440

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890010104A KR910005633B1 (ko) 1988-07-15 1989-07-15 Ldh₁의 분석법

Country Status (4)

Country Link
EP (1) EP0352547B1 (ko)
JP (1) JPH0775556B2 (ko)
KR (1) KR910005633B1 (ko)
DE (1) DE68919144T2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9333609B2 (en) 2010-12-13 2016-05-10 Doosan Infracore Co., Ltd. Tool carrier unit of a tool magazine

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5158873A (en) * 1987-05-28 1992-10-27 Abbott Laboratories Method and reagent for determining LD-1 isoenzyme
US5364765A (en) * 1987-05-28 1994-11-15 Abbott William A Method and reagent system for assaying isoenzyme profiles
WO1990001067A1 (en) * 1988-07-25 1990-02-08 Boehringer Mannheim Corporation Reagent for use in ld-1 assay
IE920779A1 (en) * 1991-03-13 1992-09-23 Du Pont Selective stabilization of lactate dehydrogenase isoenzyme¹ld1 by high molecular weight polyols
CN114381494B (zh) * 2021-12-01 2023-12-22 天津中成佳益生物科技有限公司 一种乳酸脱氢酶同工酶1的检测试剂及检测方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2513407A1 (de) * 1975-03-26 1976-10-14 Schmidt Karlheinz Ein verfahren zur differenzierung der leber- und herzspezifischen lactatdehydrogenase im blutserum
US4224406A (en) * 1978-02-27 1980-09-23 Hoffmann-La Roche Inc. Immunochemical LDH1 assay
JPS60149399A (ja) * 1984-01-12 1985-08-06 Sawao Murao アスパラギン酸アミノトランスフエラ−ゼアイソザイムの測定法
JPS62278997A (ja) * 1986-05-29 1987-12-03 Yatoron:Kk Ｌｄｈイソ酵素測定試薬
JPH0644879B2 (ja) * 1986-09-04 1994-06-15 栄研化学株式会社 ヒト血清中のグルタミン酸オキザロ酢酸トランスアミナ−ゼアイソザイムの測定法
JPH06104074B2 (ja) * 1987-03-25 1994-12-21 国際試薬株式会社 アイソザイムの分別定量法
ATE80665T1 (de) * 1987-05-28 1992-10-15 Abbott Lab Verfahren und reagens zur bestimmung von ld-1isoenzym.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9333609B2 (en) 2010-12-13 2016-05-10 Doosan Infracore Co., Ltd. Tool carrier unit of a tool magazine

Also Published As

Publication number Publication date
EP0352547B1 (en) 1994-11-02
JPH0227997A (ja) 1990-01-30
JPH0775556B2 (ja) 1995-08-16
EP0352547A3 (en) 1991-07-31
EP0352547A2 (en) 1990-01-31
DE68919144T2 (de) 1995-03-09
KR910005633B1 (ko) 1991-08-01
DE68919144D1 (de) 1994-12-08

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