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KR880011340A - 하이브리드 세르핀 및 이들이 암호화 dna - Google Patents

하이브리드 세르핀 및 이들이 암호화 dna Download PDF

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Publication number
KR880011340A
KR880011340A KR1019880002920A KR880002920A KR880011340A KR 880011340 A KR880011340 A KR 880011340A KR 1019880002920 A KR1019880002920 A KR 1019880002920A KR 880002920 A KR880002920 A KR 880002920A KR 880011340 A KR880011340 A KR 880011340A
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South Korea
Prior art keywords
hls2
exon
gene
hybrid
serpin
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KR1019880002920A
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English (en)
Inventor
라그 헤르만
프라이비쉬 게르알트
하인 프리드리히
울만 유겐
린덴마이어 베르너
Original Assignee
하이리히 벡커, 베른하르트 벡크
훽스트 아크티엔게젤샤프트
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Application filed by 하이리히 벡커, 베른하르트 벡크, 훽스트 아크티엔게젤샤프트 filed Critical 하이리히 벡커, 베른하르트 벡크
Publication of KR880011340A publication Critical patent/KR880011340A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
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  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

내용없음

Description

하이브리드 세프핀 및 이들이 암호화 DNA
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 hLS2 유전자 구조의 다이어그램.
제2도는 및 2a도;hLS2의 엑손 1내지 4를 포함하는 하이브리드 세르핀 유전자의 작제도.

Claims (12)

  1. 인간 루세르핀-2(hLS2)의 유전자 구조를 포함하는 엑손에 실질적으로 상응하는 아미노산 일부-서열을 포함하는 하이브리드 세르핀.
  2. 제1항에 있어서, hLS2-α1-안티트립신 또는 앤지오텐시노겐의 엑손에 상으하는 아미노산 일부-서열을 포함하는 하이브리드 세르핀.
  3. hLS2의 엑손/인트론 구조를 가지며 세르핀을 암호화 하는 적어도 두 개의 유전자로부터 유래된 엑손으로부터 hLS2에 상응하는 유전자 구조를 갖는 유전자를 재조합한 다음, 이 재조합 유전자를 숙주세포내에서 발현시킴을 특징으로 하여, 이 하이브리드 세르핀을 제조하는 방법.
  4. 제3항에 있어서, 숙주세포가 고등 진핵세포인 방법.
  5. hLS2에 상응하는 유전자 구조가 포함된 세르핀 유전자의 엑손을 포함하는, hLS2에 상으하는 유전자 구조를 갖는 재조합 유전자.
  6. 제5항에 있어서, 엑손이 관련 인트론의 스폴리스 시그널과 분지점에 의해 플랭킹 되는 유전자.
  7. hLS2의 엑손을 포함하는 게놈성 DNA 단편.
  8. hLS2의 게놈성 DNA.
  9. 제5항 내지 7항중 어느 한항에 따른 DNA 형질 감염된 숙주세포.
  10. 제1항 또는 2항에 따른 하이브리드 세르핀을 함유하는 약제.
  11. 제7항 또는 8항에 따른 게놈성 DNA의 전부 또는 일부를 함유하는 진단용 제제.
  12. 인간 DNA를 제7항 또는 8항에 따른 DNA 또는 상응하는 RNA와 하이브리드화시킴을 특징으로 하는 진단방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880002920A 1987-03-20 1988-03-19 하이브리드 세르핀 및 이들이 암호화 dna KR880011340A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3709255.3 1987-03-20
DE19873709255 DE3709255A1 (de) 1987-03-20 1987-03-20 Hybridserpine und dafuer kodierende dna

Publications (1)

Publication Number Publication Date
KR880011340A true KR880011340A (ko) 1988-10-27

Family

ID=6323634

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880002920A KR880011340A (ko) 1987-03-20 1988-03-19 하이브리드 세르핀 및 이들이 암호화 dna

Country Status (11)

Country Link
EP (1) EP0283932A3 (ko)
JP (1) JPS63267294A (ko)
KR (1) KR880011340A (ko)
AU (1) AU611676B2 (ko)
DE (1) DE3709255A1 (ko)
DK (1) DK151788A (ko)
FI (1) FI881268L (ko)
HU (1) HUT46740A (ko)
IL (1) IL85789A0 (ko)
PT (1) PT87009B (ko)
ZA (1) ZA881948B (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0730660A4 (en) * 1993-10-29 1998-02-25 Incyte Pharma Inc CHIMEAN PROTEINE PROTEASE NEXIN-1 CONTAINING VARIANTS
DE19742725A1 (de) * 1997-09-26 1999-04-01 Abts Harry Frank Dr Hurpin/PI13: Ein neuer, UV-reprimierbarer, Serin Protease Inhibitor aus der Familie der Ovalbumin-Serpine
US7247704B2 (en) 2000-12-18 2007-07-24 Arriva Pharmaceuticals, Inc. Multifunctional protease inhibitors and their use in treatment of disease
JP4426179B2 (ja) * 2000-12-18 2010-03-03 アライバ ファーマシューティカルズ，インコーポレイティド 多機能性プロテアーゼインヒビターおよびそれらの疾患治療における使用
EP1903113A1 (en) * 2000-12-18 2008-03-26 Arriva Pharmaceuticals, Inc. Multifunctional protease inhibitors and their use in treatment of disease

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3521226A1 (de) * 1985-02-08 1986-08-14 Hoechst Ag, 6230 Frankfurt Gene fuer biologisch aktive proteine

Also Published As

Publication number Publication date
PT87009B (pt) 1992-06-30
DE3709255A1 (de) 1988-09-29
ZA881948B (en) 1988-12-28
AU1328888A (en) 1988-09-22
FI881268L (fi) 1988-09-21
PT87009A (pt) 1988-04-01
EP0283932A3 (de) 1989-10-25
JPS63267294A (ja) 1988-11-04
EP0283932A2 (de) 1988-09-28
FI881268A0 (fi) 1988-03-17
HUT46740A (en) 1988-11-28
DK151788A (da) 1988-09-21
DK151788D0 (da) 1988-03-18
IL85789A0 (en) 1988-09-30
AU611676B2 (en) 1991-06-20

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