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KR870010188A - 핵산 또는 엔도톡신의 제거제 및 제거방법 - Google Patents

핵산 또는 엔도톡신의 제거제 및 제거방법 Download PDF

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KR870010188A
KR870010188A KR870002578A KR870002578A KR870010188A KR 870010188 A KR870010188 A KR 870010188A KR 870002578 A KR870002578 A KR 870002578A KR 870002578 A KR870002578 A KR 870002578A KR 870010188 A KR870010188 A KR 870010188A
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endotoxin
nucleic acid
low molecular
molecular weight
removal method
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KR940005589B1 (ko
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마사노리 하시모도
다께시 야마모도
도오루 가와찌
준지 구와시마
요오이찌 기다오까
Original Assignee
후지하라 도미오
다이닛뽄세이야꾸 가부시끼가이샤
나까무라 사다오
구리다 고오교오 가부시끼 가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
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  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • External Artificial Organs (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

핵산 또는 엔도톡신의 제거제 및 제거방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (18)

  1. 저분자키토산을 유효성분으로서 함유하는 것을 특징으로 하는 핵산 또는 엔도톡신의 제거제.
  2. 제 1항에 있어서, 저분자 키토산이 고유점도 0.01 내지 5(d1/g)인 제거제.
  3. 제 1항 또는 제 2항에 있어서, 저분자 키토산이 pH4에 있어서의 콜로이드당량 2meq/g-증발잔분이상인 제거제.
  4. 제 1항 내지 제 3항 중의 어느 하나에 있어서, 저분자 키토산이 고유점도 0.2 내지 1(d1/g), pH4에 있어서의 콜로이드당량 4meq/g-증발잔분이상인 제거제.
  5. 제 1항 내지 제 3항 중의 어느 하나에 있어서, 제거제가 핵산 또는 엔도톡신을 함유하는 피처리액으로부터 핵산 또는 엔도톡신을 분리하는 제거제.
  6. 제 5항에 있어서, 피처리액이 등전점 3 이상의 단백질류를 함유하는 제거제.
  7. 제 6항에 있어서, 단백질류가 사람암괴사인자인 제거제.
  8. 제 6항에 있어서, 단백질류가 사람인터로이킨 1인 제거제.
  9. 제 5항 내지 제 8항 중의 어느 하나에 있어서, 피처리액이 무세포추출액인 제거제.
  10. 저분자 키토산을 사용하여 핵산 또는 엔도톡신을 분리하는 것을 특징으로 하는 핵산 또는 엔도톡신의 제거방법.
  11. 제10항에 있어서, 저분자키토산이 고유점도 0.01 내지 5(dl/g)인 제거방법.
  12. 제10항 또는 제11항에 있어서, 저분자키토산이 pH4에 있어서의 콜로이드당량 2meq/g-증발잔분이상인 제거방법.
  13. 제10항 내지 제12항중 어느하나에 있어서, 저분자키토산이 고유점도 0.2 내지 1(d1/g), pH4에 있어서의 콜로이드당량 4meq/g-증발잔분이상인 제거방법.
  14. 제10항 내지 제12항 중의 어느 하나에 있어서 분리가 핵산 또는 엔도톡신을 함유한 피처리액으로부터 핵산 또는 엔도톡신을 분리하는 제거방법.
  15. 제14항에 있어서, 피처리액이 등전점 3 이상의 단백질류를 함유하는 제거방법.
  16. 제15항에 있어서, 단백질류가 사람암괴사인자인 제거방법.
  17. 제15항에 있어서, 단백질류가 사람인터로이킨 1인 제거방법.
  18. 제14항 내지 제16항 중의 어느 하나에 있어서, 퍼처리액이 무세포추출액인 제거방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870002578A 1986-04-02 1987-03-21 핵산 또는 엔도톡신의 제거제 및 제거방법 Expired - Fee Related KR940005589B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP75779 1986-04-02
JP???61-75779 1986-04-02
JP7577986 1986-04-02

Publications (2)

Publication Number Publication Date
KR870010188A true KR870010188A (ko) 1987-11-30
KR940005589B1 KR940005589B1 (ko) 1994-06-21

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ID=13586043

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KR1019870002578A Expired - Fee Related KR940005589B1 (ko) 1986-04-02 1987-03-21 핵산 또는 엔도톡신의 제거제 및 제거방법

Country Status (8)

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US (1) US4885168A (ko)
EP (1) EP0240348B1 (ko)
JP (1) JPH0641479B2 (ko)
KR (1) KR940005589B1 (ko)
AT (1) ATE96448T1 (ko)
AU (1) AU596160B2 (ko)
CA (1) CA1291594C (ko)
DE (1) DE3787913T2 (ko)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0720984B2 (ja) * 1989-09-22 1995-03-08 栗田工業株式会社 エンドトキシンの除去方法
US5169535A (en) * 1989-09-22 1992-12-08 Kurita Water Industries Ltd. Method of removing endotoxin
JPH0687974B2 (ja) * 1990-03-27 1994-11-09 忠一 平山 発熱物質の吸着材料
JP2690415B2 (ja) * 1991-01-26 1997-12-10 政志 船山 発熱性物質吸着体とそれを用いた発熱性物質の定量方法
JPH06192296A (ja) * 1992-10-28 1994-07-12 Chiba Pref Gov 治療用医薬品としての結晶ａ型ボツリヌス毒素の製造法。
US5972225A (en) * 1996-05-07 1999-10-26 Cook Imaging Corporation Process for remediating endotoxin-contaminated bulk non-ionic contrast media
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6194562B1 (en) 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
WO2001081429A1 (en) * 2000-04-21 2001-11-01 Johns Hopkins University Differential microbial test
US6716970B2 (en) * 2000-04-21 2004-04-06 Adjuvant Pharmaceuticals, Llc Water soluble, randomly substituted partial N-partial O-acetylated chitosan, preserving compositions containing chitosan, and processes for making thereof
SE0003958D0 (sv) 2000-10-31 2000-10-31 Biogaia Fermentation Ab Method for growth of microorganisms
AU2002349439A1 (en) * 2001-12-26 2003-07-15 Nihon Medi-Physics Co., Ltd. Compositions for eliminating endotoxin and elimination method
US7125967B2 (en) * 2003-10-08 2006-10-24 Adjuvant Pharmaceuticals, Llc Water-soluble chitosan having low endotoxin concentration and methods for making and using the same
EP1963526A4 (en) 2005-12-09 2009-11-18 Promega Corp PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
CN102977392A (zh) * 2012-12-17 2013-03-20 上海典范医疗科技有限公司 去除内毒素的细菌纤维素的制备方法
US9598504B2 (en) 2014-12-16 2017-03-21 Chitogen Inc. Process for the removal of endotoxins to produce medical quality chitosan and other polysaccharides
US20220315622A1 (en) 2019-09-06 2022-10-06 Bia Separations D.O.O. Compositions and methods for reducing chromatin content of biological preparations
CN112746065A (zh) * 2020-12-24 2021-05-04 四川德博尔制药有限公司 一种胃蛋白酶内毒素的去除方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3922260A (en) * 1973-08-24 1975-11-25 Quintin P Peniston Process for depolymerization of chitosan
JPS54140710A (en) * 1978-03-10 1979-11-01 Mitsui Toatsu Chem Inc Anti-tumor substance and its preparation
US4605623A (en) * 1982-11-08 1986-08-12 Malette William Graham Method of altering growth and development and suppressing contamination microorganisms in cell or tissue culture
US4474769A (en) * 1983-05-13 1984-10-02 Pfanstiehl Laboratories, Inc. Chitosan as a contraceptive
DE19975063I2 (de) * 1984-03-06 2005-08-18 Dainippon Pharmaceutical Co DNS den menschlichen Tumornekrosisfaktor kodierendund das menschliche Tumornekronisfaktor-Polypepti d.

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Publication number Publication date
EP0240348A3 (en) 1989-10-04
EP0240348A2 (en) 1987-10-07
AU7096487A (en) 1987-10-08
DE3787913T2 (de) 1994-03-24
KR940005589B1 (ko) 1994-06-21
JPH0641479B2 (ja) 1994-06-01
AU596160B2 (en) 1990-04-26
DE3787913D1 (de) 1993-12-02
ATE96448T1 (de) 1993-11-15
EP0240348B1 (en) 1993-10-27
CA1291594C (en) 1991-10-29
US4885168A (en) 1989-12-05
JPS6356300A (ja) 1988-03-10

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