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KR860008286A - 단백질 발현을 증가시키기 위한 trp 오페론의 샤인-달가노 서열과 개시 코돈 사이 DNA 서열의 변형 - Google Patents

단백질 발현을 증가시키기 위한 trp 오페론의 샤인-달가노 서열과 개시 코돈 사이 DNA 서열의 변형 Download PDF

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KR860008286A
KR860008286A KR1019860003040A KR860003040A KR860008286A KR 860008286 A KR860008286 A KR 860008286A KR 1019860003040 A KR1019860003040 A KR 1019860003040A KR 860003040 A KR860003040 A KR 860003040A KR 860008286 A KR860008286 A KR 860008286A
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shine
vector
cleavage site
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KR940004543B1 (ko
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엔젤스 요아힘
레이네베베르 미카엘
울만 유진
벤진마이어 프리드리히
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훽스트 아크티엔게젤샤프트
베른하르트 벡, 하인리히 벡커
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
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    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)

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Abstract

내용 없음

Description

단백질 발현을 증가시키기 위한 trp 오페론의 샤인-달가노 서열과 개시 코돈 사이 DNA 서열의 변형
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라스미드 ptrpl 1으로부터 플라스미드 pH 131/5의 제조를 나타내고,
제2도는 플라스미드 p 159/6 및 플라스미드 pH 131/5로부터 인터루킨 -2를 암호화하는 플라스미드 pH 185/11의 제조를 보여주며,
제3도는 β-인터페론을 암호화하는 플라스미드 pH 192/5를 나타낸다.

Claims (3)

  1. 5'ATGG 3'가 뒤따르는 DNA 서열(I) (암호화 본쇄 : coding strand)을 함유하는 이. 콜라이(E.coli) 벡터를 제한효소 NcoI으로 절단하고,
    a) 절단 부위에 DNA 서열(Ⅱ)의 유전자 구조를 연결시키거나, 또는
    b) 절단 부위를 효소학적으로 채우고 서열(Ⅲ)의 DNA를 서열(Ⅳ)의 DNA로 연결시키거나, 또는
    c) 절단 부위의 돌출 서열을 효소학적으로 분해하고, 서열(Ⅵ)의 DNA를 서열(Ⅶ)의 DNA로 열결시킴을 특징으로하여, 이. 콜라이의 trp 발현 시스템을 갖는 벡터를 제조하는 방법.
    5' GTATCGACC 3' (Ⅰ)
    5' CATGX… 3' (Ⅱ)
    3' Y… 5'
    5' GTA TCG ACC ATG 3' (Ⅲ)
    3' CAT AGC TGG TAC 5'
    5' X… 3' (Ⅳ)
    3' Y… 5'
    5' GTA TCG AC 3' (Ⅵ)
    3' CAT AGC TG 5'
    5' Z ATG X… 3' (Ⅶ)
    3' Z'TAC Y… 5'
    상기 서열에서, X 및 Y는 구조 유전자 개시 코돈(start codon)의 하향적으로 첫번째 상보적인 누클레오티드 쌍을 나타내고, Z 및 Z'는 어느 목적하는 누클레오티드 쌍을 나타내나 또한 없을 수도 있다.
  2. 제1항에 있어서, 샤인-달가노 서열(shine-Dalgarno sequence) AAGG에 5' 말단이 연결되고, 3' 말단의 G가 개시 코돈의 하향 구조 유전자의 첫번째 누클레오티드인 서열(암호화 본쇄) (Ia)를 서열(I)의 위치에 삽입시킨 방법.
    5' GTATCGACCATGG 3' (Ia)
  3. 이. 콜라이 세포에서 제1 또는 2항에서 정의한 벡터의 발현을 유도하여 유전적으로 암호될 수 있는 아미노산으로 이루어진 폴리펩티드를 제조하는 방법.
    .※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860003040A 1985-04-19 1986-04-19 이.콜라이의 trp 발현 시스템을 갖는 벡터를 제조하는 방법 Expired - Lifetime KR940004543B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3514113.1 1985-04-19
DE19853514113 DE3514113A1 (de) 1985-04-19 1985-04-19 Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression

Publications (2)

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KR860008286A true KR860008286A (ko) 1986-11-14
KR940004543B1 KR940004543B1 (ko) 1994-05-25

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EP (1) EP0198415B1 (ko)
JP (1) JPH0665317B2 (ko)
KR (1) KR940004543B1 (ko)
AT (1) ATE44046T1 (ko)
AU (1) AU600229B2 (ko)
CA (1) CA1321963C (ko)
DE (2) DE3514113A1 (ko)
DK (1) DK172695B1 (ko)
ES (2) ES8704541A1 (ko)
FI (1) FI84362C (ko)
GR (1) GR861022B (ko)
HU (1) HU196458B (ko)
IE (1) IE58994B1 (ko)
IL (1) IL78529A (ko)
NO (1) NO175646C (ko)
NZ (1) NZ215858A (ko)
PH (1) PH26596A (ko)
PT (1) PT82417B (ko)
ZA (1) ZA862925B (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966849A (en) * 1985-09-20 1990-10-30 President And Fellows Of Harvard College CDNA and genes for human angiogenin (angiogenesis factor) and methods of expression
AU8379991A (en) * 1990-09-14 1992-03-26 Astra Aktiebolag A novel method of generating clones for the expression of unfused proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3247922A1 (de) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
DE3430683A1 (de) * 1984-08-21 1986-03-06 Hoechst Ag, 6230 Frankfurt Synthetische regulationsregion

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Publication number Publication date
NO175646B (ko) 1994-08-01
FI84362C (fi) 1991-11-25
ATE44046T1 (de) 1989-06-15
PT82417A (de) 1986-05-01
NZ215858A (en) 1989-01-06
DE3663956D1 (en) 1989-07-20
GR861022B (en) 1986-08-18
ES554097A0 (es) 1987-04-01
JPH0665317B2 (ja) 1994-08-24
JPS61242583A (ja) 1986-10-28
KR940004543B1 (ko) 1994-05-25
NO175646C (no) 1994-11-09
FI861624A0 (fi) 1986-04-17
ES8704541A1 (es) 1987-04-01
HU196458B (en) 1988-11-28
DK179686A (da) 1986-10-20
HUT41068A (en) 1987-03-30
AU600229B2 (en) 1990-08-09
ES8706818A1 (es) 1987-07-01
EP0198415A2 (de) 1986-10-22
DK179686D0 (da) 1986-04-18
EP0198415B1 (de) 1989-06-14
AU5638786A (en) 1986-10-23
ZA862925B (en) 1986-12-30
DE3514113A1 (de) 1986-10-23
FI861624L (fi) 1986-10-20
CA1321963C (en) 1993-09-07
FI84362B (fi) 1991-08-15
IL78529A (en) 1991-07-18
NO861551L (no) 1986-10-20
IE861031L (en) 1986-10-19
PT82417B (pt) 1988-08-17
DK172695B1 (da) 1999-05-31
IL78529A0 (en) 1986-08-31
EP0198415A3 (en) 1986-12-30
ES556233A0 (es) 1987-07-01
IE58994B1 (en) 1993-12-15
PH26596A (en) 1992-08-19

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