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KR840008692A - 미생물 단백질 분리물의 정제방법 - Google Patents

미생물 단백질 분리물의 정제방법 Download PDF

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Publication number
KR840008692A
KR840008692A KR1019840002045A KR840002045A KR840008692A KR 840008692 A KR840008692 A KR 840008692A KR 1019840002045 A KR1019840002045 A KR 1019840002045A KR 840002045 A KR840002045 A KR 840002045A KR 840008692 A KR840008692 A KR 840008692A
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KR
South Korea
Prior art keywords
treated
acid
aqueous solution
protein isolate
process according
Prior art date
Application number
KR1019840002045A
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English (en)
Inventor
샤르프(외 1) 우도
Original Assignee
하인리히 베커, 베른하르트 베크
훽스트 아크티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 하인리히 베커, 베른하르트 베크, 훽스트 아크티엔게젤샤프트 filed Critical 하인리히 베커, 베른하르트 베크
Publication of KR840008692A publication Critical patent/KR840008692A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/008—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/24—Organic nitrogen compounds
    • A21D2/26—Proteins
    • A21D2/267—Microbial proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/823—Lower fungi, e.g. mold
    • Y10S530/824—Yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음

Description

미생물 단백질 분리물의 정제방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (14)

  1. 미생물 단백질 분리물을 인산염 및/또는 하이드록시, 포르밀, 케토 및 카복시중의 적어도 2개의 반응성 그룹을 함유하는 친수성 저분자량 지방족 화합물을 함유하는 수용액으로 처리함을 특징으로하여, 미생물 단백질 분리물을 정제하는 방법.
  2. 제1항에 있어서, 단백질 분리물을 방항성산, 당류 또는 폴리올의 수용액으로 처리함을 특징으로 하는 방법.
  3. 제1항에 있어서, 인산염이 나트륨 또는 칼륨오르토인산염 또는 이인산염임을 특징으로 하는 방법.
  4. 제1항 또는 2항에 있어서, 산 반응성이 있는 지방족 화합물이 알칼리토금속염 또는 알카리금속염의 형태로 사용됨을 특징으로 하는 방법.
  5. 제1항 내지 4항중 어느 하나에 있어서, 미생물 단백질 분리물이 핵산을 함유한 탈지 세포 집합체임을 특징으로 하는 방법.
  6. 제1항 내지 5항중 어느 하나에 있어서, 동일 또는 상이한 추출제로 한단계 이상 처리함을 특징으로 하는 방법.
  7. 제1항 내지 6항중 어느 하나에 있어서, 수용액이 락트산, 말산 또는 시트르산을 함유함을 특징으로 하는 방법.
  8. 제1항 내지 7항중 어느 하나에 있어서, 제1단계에서는 락트산으로 처리하고, 후속 단계에서는 슈크로즈 용액으로 처리함을 특징으로 하는 방법.
  9. 제1항 내지 8항중 어느 하나에 있어서, 약 0내지 100℃에서 처리함을 특징으로 하는 방법.
  10. 제1항 내지 9항중 어느 하나에 있어서, 20 내지 90℃에서 처리함을 특징으로 하는 방법.
  11. 제1항 내지 10항중 어느 하나에 있어서, 40 내지 80℃에서 처리함을 특징으로 하는 방법.
  12. 제1항 내지 11항중 어느 하나에 있어서, 인산염 또는 지방족 화합물을 0.1 내지 3중량%함유하는 수용액으로 처리함을 특징으로 하는 방법.
  13. 제12항에 있어서, 농도가 0.2 내지 2%임을 특징으로 하는 방법.
  14. 제12항에 있어서, 농도가 0.5 내지 1%임을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원내용에 의하여 공개하는 것임.
KR1019840002045A 1983-04-20 1984-04-18 미생물 단백질 분리물의 정제방법 KR840008692A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19833314292 DE3314292A1 (de) 1983-04-20 1983-04-20 Verfahren zur reinigung von mikrobiellen proteinisolaten
DEP3314292,0 1983-04-20

Publications (1)

Publication Number Publication Date
KR840008692A true KR840008692A (ko) 1984-12-17

Family

ID=6196863

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019840002045A KR840008692A (ko) 1983-04-20 1984-04-18 미생물 단백질 분리물의 정제방법

Country Status (18)

Country Link
US (1) US4530785A (ko)
EP (1) EP0126289A3 (ko)
JP (1) JPS59198992A (ko)
KR (1) KR840008692A (ko)
AU (1) AU2710984A (ko)
BG (1) BG38635A3 (ko)
DE (1) DE3314292A1 (ko)
DK (1) DK120784A (ko)
ES (1) ES8501960A1 (ko)
FI (1) FI841560L (ko)
GR (1) GR79931B (ko)
HU (1) HUT34547A (ko)
IL (1) IL71580A (ko)
NO (1) NO841559L (ko)
PL (1) PL247335A1 (ko)
PT (1) PT78447B (ko)
RO (1) RO90644B (ko)
ZA (1) ZA842961B (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4675387A (en) * 1985-07-26 1987-06-23 E. I. Du Pont De Nemours And Company Method for extracting protein with organic acid

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1525005A (fr) * 1966-04-08 1968-05-17 Inst Francais Du Petrole Procédé d'amélioration de la qualité alimentaire des microorganismes
FR1591574A (ko) * 1968-05-10 1970-05-04
IT954172B (it) * 1970-07-24 1973-08-30 Standard Oil Co Procedimento per estrarre materiale proteico da organismi microbici unicellulari e prodotto ottenuto
JPS4832671B1 (ko) * 1970-10-28 1973-10-08
US3781264A (en) * 1970-10-29 1973-12-25 Standard Oil Co Process for texturizing microbial cells by alkali-acid treatment
US3819610A (en) * 1970-10-29 1974-06-25 C Akin Process for preparing polycellular protein products
US3833552A (en) * 1971-02-04 1974-09-03 Standard Oil Co Water-soluble whippable protein material and process for producing same
US3784536A (en) * 1971-02-04 1974-01-08 Standard Oil Co Process for reducing the nucleic acid content of single cell protein affording microorganisms
US3885050A (en) * 1972-09-01 1975-05-20 Standard Oil Co Treatment of protein - containing microbial cells to remove undesirable flavor and odor substances
US3962466A (en) * 1972-11-10 1976-06-08 Dai-Nippon Sugar Manufacturing Co., Ltd. Method for treatment of microorganisms
SE7401668L (ko) * 1974-02-07 1975-08-08 Scp Exploatering Ab
US3891772A (en) * 1974-02-13 1975-06-24 Standard Oil Co Extraction of undesirable flavor and odor components from microbial cells
GB1498688A (en) * 1975-07-16 1978-01-25 Ici Ltd Treatment of single cell protein
US4206243A (en) * 1976-07-27 1980-06-03 Hoechst Aktiengesellschaft Process for reducing the contents of lipids and nucleic acid in microbial cell masses
CA1079568A (en) * 1976-08-02 1980-06-17 Erich H. Schuldt (Jr.) Process for reducing the ribonucleic acid content of yeast protein
JPS545092A (en) * 1977-06-14 1979-01-16 Miyarisan Pharma Production of odor free strains
DD152690A3 (de) * 1978-09-20 1981-12-09 Kraatz Karl Heinz Verfahren zur reduzierung des nucleinsaeuregehaltes mikrobieller biomasse
US4341802A (en) * 1980-10-24 1982-07-27 Provesto Corporation Production of protein with reduced nucleic acid
US4330464A (en) * 1981-07-09 1982-05-18 George Weston Limited Isolation of microbial protein with reduced nucleic acid content
US4472302A (en) * 1983-03-09 1984-09-18 Merck & Co., Inc. Heat shock process for the isolation of bacterial protein

Also Published As

Publication number Publication date
IL71580A0 (en) 1984-07-31
PL247335A1 (en) 1985-07-16
EP0126289A2 (de) 1984-11-28
DK120784D0 (da) 1984-02-28
US4530785A (en) 1985-07-23
ZA842961B (en) 1984-11-28
BG38635A3 (bg) 1986-01-15
HUT34547A (en) 1985-03-28
FI841560L (fi) 1984-10-21
ES531730A0 (es) 1984-12-16
FI841560A0 (fi) 1984-04-18
RO90644B (ro) 1987-01-31
AU2710984A (en) 1984-10-25
GR79931B (ko) 1984-10-31
PT78447B (de) 1986-06-02
PT78447A (de) 1984-05-01
DE3314292A1 (de) 1984-10-25
ES8501960A1 (es) 1984-12-16
EP0126289A3 (de) 1987-01-14
DK120784A (da) 1984-10-21
JPS59198992A (ja) 1984-11-10
NO841559L (no) 1984-10-22
RO90644A (ro) 1987-01-30
IL71580A (en) 1986-12-31

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Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19840418

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid