[go: up one dir, main page]

KR840005745A - B형 간염비루스 유전자를 삽입한 조환 플라스미드, 이 조환 플라스미드로 전환된 효모 및 b형 간염비루스 표면항원의 제조방법 - Google Patents

B형 간염비루스 유전자를 삽입한 조환 플라스미드, 이 조환 플라스미드로 전환된 효모 및 b형 간염비루스 표면항원의 제조방법 Download PDF

Info

Publication number
KR840005745A
KR840005745A KR1019830003794A KR830003794A KR840005745A KR 840005745 A KR840005745 A KR 840005745A KR 1019830003794 A KR1019830003794 A KR 1019830003794A KR 830003794 A KR830003794 A KR 830003794A KR 840005745 A KR840005745 A KR 840005745A
Authority
KR
South Korea
Prior art keywords
gene
plasmid
yeast
phosphatase
coli
Prior art date
Application number
KR1019830003794A
Other languages
English (en)
Other versions
KR890000096B1 (ko
Inventor
아쓰씨 미야노하라
아쓰시 미야노하라 (외 5)
지까데루 노자끼
후꾸사부로오 하마다
아끼오 도오에
노부야 오오도모
겡이찌 마쓰바라
Original Assignee
자이단호오진 가가구 오요비겟세이 료오호오겡뀨우쇼
로꾸단다 도오끼찌
자이단호오진 가가구 오요비겟세이 료오호오 껭뀨우쇼
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 자이단호오진 가가구 오요비겟세이 료오호오겡뀨우쇼, 로꾸단다 도오끼찌, 자이단호오진 가가구 오요비겟세이 료오호오 껭뀨우쇼 filed Critical 자이단호오진 가가구 오요비겟세이 료오호오겡뀨우쇼
Publication of KR840005745A publication Critical patent/KR840005745A/ko
Application granted granted Critical
Publication of KR890000096B1 publication Critical patent/KR890000096B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

내용 없음

Description

B형 간염비루스 유전자를 삽입한 조환 플라스미드, 이 조환 플라스미드로 전환된 효모 및 B형 간염비루스 표면항원의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명에서 사용되는 HBV 유전자를 함유하는 HBV DNA 단편의 구조도.
제2도 (1),(2),(3)은 HBs 유전자의 아미노산 배열도.
제3도는 셔틀벡터 PAT 77 및 AM 82의 구조도.

Claims (23)

  1. 효모의 유전자와 대장균의 유전자를 함유하고 포스파티아제 촉진유전자의 조절하에 조환되는 효모의 억제성 산성 포스파티아제 유전자의 발현조절영역과 B형 간염비루스 유전자를 나르는 플라스미드 벡터로 된 조환 플라스미드.
  2. 제1항에 있어서, 전술한 효모의 억제성 산성 포스파타아제의 유전자의 발현조절영역이 포스파타아제를 구성하는 60,000달톤(P60)의 폴리펩티드의유전자이며, 포스파타아제의 구조유전자의 일부 또는 전부 및 임의의 상류의 어떤 영역이 제거되어 있는 조환 플라스미드.
  3. 제2항에 있어서, 전부의 포스파타아제 구조유전자에 대해서 포스파타아제 구제유전자의 상류에서 제거될 영역이 =1(ATG)에서 -100bp 범위 이내인 조환 플라스미드.
  4. 제3항에 있어서, 제거될 영역이 +1에서 -50bp 범위 이내인 조환 플라스미드.
  5. 제1항에 있어서, 전술한 효모의 유전자가 ars 1 및 2μ ori를 함유하는 조환 플라스미드.
  6. 제1항에 있어서, 전술한 효모의 유전자가 ars 1, 2μori 및 전환효모의 선택표식 유전자를 가지고 있는 조환 플라스미드.
  7. 제6항에 있어서, 전술한 선택표식 유전자가 로이신 생선유전자, 히스티딘 생산유전자, 트립토판 생산유전자, 우라실 생산유전자, 아대닌 생산유전자로 된 군에서 일원 및 이것의 둘 또는 그 이상의 결합인 조환 플라스미드.
  8. 제1항에 있어서, 전술한 대장균의 유전자가 대장균의 세포 내에서 유전자의 증식에 필요한 DNA 배열을 가지고 있는 조혼 플라스미드.
  9. 제1항에 있어서, 전술한 대장균의 유전자가 대장균의 세포 내에서 유전자의 증식에 필요한 DNA 배열과 전환 대장균으 선택표시 유전자를 가지고 있는 조환 플라스미드.
  10. 제9항에 있어서, 선택표식 유전자가 암피실린 내성유전, 자카나마이신 내성유전자, 테트라사이클린 내성유전자 및 클로람페니콜 내성유전자로 된 균에서 선택되는 일원 및 이것을 둘 또는 그 이상의 결합인 조환 플라스미드.
  11. 제9항에 있어서, 전술한 대장균의 유전자가 암피실린 내성유전자와 테트라사이클린 내성유전자를 함유하는 대장균 플라스미드 pBR 322인 조환 플라스미드.
  12. 제1항에 있어서, 전술한 플라스미드 벡터가 효모 플라스미드 및 대장균 플라스미드로 된 셔틀벡터이고, 이 효모플라스미드가 일부 또는 전부의 산성 포스파타아제 구조유전자와 이 포스파타아제 구조유전자의 상류의 어떤 영역이 제거되는데, ars 1, 2μori, Leu 2 및 억제성산성 포스파타아제 유전자의 발현조절영역을 이 순서대로 함유하고 있고 또 전술한 대장균 플라스미드가 암피실린 내성유전자와 테트라사이클린 내성유전자를 가진 대장균 플라스미드 pBR 322인 조환 플라스미드.
  13. 제12항에 있어서, 전술한 효모 플라스미드가 산성 포스 파타아제 구조유전자의 전부와 이 포스파타아제 구조유전자상류의 영역이 +1에서 -50bp 범위내에서 결핍되어 있는 조환 플라스미드.
  14. 제13항에 있어서 포스파타아제 구조유전자 상류의 전술한 결핍영역이 +1내지 -50bp 범위내인조환 플라스미드.
  15. 제1항에 있어서, 플라스미드 벡터와 조환될 전술한 B형 간염비루스의 유전자가 226아미노산에 대응하는 HBs 유전자의 전부를 함유하는 단편인 조환 플라스미드.
  16. 제15항에 있어서, HBs 유전자의 전부를 함유한 전술한 단편이 제한효소 Xho I로 개열되는 HBV DNA의 전부인 조환 플라스미드.
  17. 제15항에 있어서, HBs 유전자의 전부를 함유하는 전술한 단편이 HBV DNA를 제한효소 Xho I 및 BamHI로 절단시켜서 제조한 단편인 조환 플라스미드.
  18. 제1항에 있어서 설명한 조환 플라스미드로 효모를 전환시켜서 제조한 전환효모.
  19. 제18항에 있어서, 전술한 출발재료가 로이신 요구성 돌연변이 균주, 히스리딘 요구성 돌연변이 균주, 트립토판 요구성 돌연변이 균주, 우리실 요구성 돌연변이 균주 및 아데닌 요구성 돌연변이 균주로 된군에서 선택되는 일원의 전환효모.
  20. 제18항에 있어서, 출발재료가 로이신 요구성 돌연변이 균주; 시카로미세스 세레비시아에 〔a, Leu 2, his 4, can 1(Cir+)〕인 전환효모.
  21. 제18항에 있어서, 설명한 전환효모를 배지에서 배양하고 또 생성된 항원을 종래방법으로 수집하는 것으로 B형 간염비루스 표면항원의 제조방법.
  22. 제21항에 있어서, 전환효모의 배양이 산성 포스파타아제 촉진유전자가 억제되지 않는 조건하에 수행되는 방법.
  23. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019830003794A 1982-08-16 1983-08-13 B형 간염 비루스 표면항원의 제조방법 KR890000096B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP57142460A JPS5931799A (ja) 1982-08-16 1982-08-16 Ｂ型肝炎ウイルス遺伝子を組込んだ組換えプラスミド
JP142460 1982-08-16
JP82-142460 1982-08-16

Publications (2)

Publication Number Publication Date
KR840005745A true KR840005745A (ko) 1984-11-15
KR890000096B1 KR890000096B1 (ko) 1989-03-07

Family

ID=15315829

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019830003794A KR890000096B1 (ko) 1982-08-16 1983-08-13 B형 간염 비루스 표면항원의 제조방법

Country Status (8)

Country Link
US (1) US4778761A (ko)
JP (1) JPS5931799A (ko)
KR (1) KR890000096B1 (ko)
CA (1) CA1270217A (ko)
DK (1) DK169953B1 (ko)
ES (2) ES524942A0 (ko)
IL (1) IL69423A0 (ko)
SU (1) SU1387878A3 (ko)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
JPS5936699A (ja) * 1982-08-20 1984-02-28 Chemo Sero Therapeut Res Inst シヤトルベクタ−
JPS5974985A (ja) * 1982-10-19 1984-04-27 Takeda Chem Ind Ltd 新規ｄｎａ
JPS61139391A (ja) * 1984-12-11 1986-06-26 Chemo Sero Therapeut Res Inst 単純ヘルペスウィルス遺伝子を組込んだ組換えプラスミドおよび形質転換酵母
US4816564A (en) * 1986-01-31 1989-03-28 Merck & Co., Inc. Method for producing hepatitis B virus proteins in yeast
US5190877A (en) * 1987-09-03 1993-03-02 Gist-Brocades N.V. Saccharomyces strains for maltose fermentation
US4883865A (en) * 1987-09-30 1989-11-28 Merck & Co. Inc. Recovery of pres2+s antigen
AP129A (en) * 1988-06-03 1991-04-17 Smithkline Biologicals S A Expression of retrovirus gag protein eukaryotic cells
US6319501B1 (en) * 1990-07-19 2001-11-20 Health Research, Inc. Method of immunizing against hepatitis B virus
LT3988B (en) 1992-02-17 1996-06-25 Fermentas Biotech Inst Recombinant plasmides pfs19, pfps2-48 and pjlfds1 codingsynthesis of human hepatite b of surfice virus antigenes, methods fof producing thereof
US5531990A (en) * 1993-12-15 1996-07-02 Health Research, Inc. Method of raising an immune response with an anti-idiotypic antibody having correspondence with human hepatitis B surface antigen
IL118626A0 (en) * 1996-06-11 1996-10-16 Xtl Biopharmaceuticals Limited Anti HBV antibody
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
CA2365418C (en) 1999-03-31 2009-05-26 Cornell Research Foundation, Inc. Phosphatases with improved phytase activity
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
AU2002356880A1 (en) 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
WO2004024885A2 (en) * 2002-09-13 2004-03-25 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
EP2069486A2 (en) * 2006-08-03 2009-06-17 Cornell Research Foundation, Inc. Phytases with improved thermal stability
MX2009011851A (es) 2007-05-02 2010-03-04 Merial Ltd Plasmidos de dna que tienen expresion y estabilidad mejoradas.
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
CN112680464B (zh) * 2020-12-10 2022-04-26 南京农业大学 一种单甲基砷和三价锑氧化酶基因arsV及其编码的蛋白和应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2480779B2 (fr) * 1979-08-30 1986-07-18 Anvar Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur
FR2444713A1 (fr) * 1978-12-18 1980-07-18 Pasteur Institut Procede de production d'un adn comprenant le genome du virus de l'hepatite b et vecteur le comportant
IE54046B1 (en) * 1981-08-25 1989-05-24 Celltech Ltd Expression vectors
US4446235A (en) * 1982-03-22 1984-05-01 Genentech, Inc. Method for cloning human growth hormone varient genes
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
AU584580B2 (en) * 1982-09-08 1989-06-01 Smith Kline - Rit Hepatitis B virus vaccine
CA1341116C (en) * 1983-02-22 2000-10-17 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis or foreign protein

Also Published As

Publication number Publication date
DK372183D0 (da) 1983-08-15
JPS5931799A (ja) 1984-02-20
DK372183A (da) 1984-02-17
SU1387878A3 (ru) 1988-04-07
ES8600059A1 (es) 1985-10-01
KR890000096B1 (ko) 1989-03-07
CA1270217A (en) 1990-06-12
IL69423A0 (en) 1983-11-30
US4778761A (en) 1988-10-18
ES524942A0 (es) 1985-10-01
DK169953B1 (da) 1995-04-10
ES8802162A1 (es) 1988-04-01
ES540895A0 (es) 1988-04-01
JPS6155950B2 (ko) 1986-11-29

Similar Documents

Publication Publication Date Title
KR840005745A (ko) B형 간염비루스 유전자를 삽입한 조환 플라스미드, 이 조환 플라스미드로 전환된 효모 및 b형 간염비루스 표면항원의 제조방법
BG60107B2 (bg) Метод за получаване на еукариотен полипептид
KR870002257A (ko) 히루딘을 유전공학적으로 제조하는 방법
CA2467648A1 (en) Ovomucoid promoter and methods of use
RU2004107694A (ru) Способ интеграции генов в специфических сайтах в клетках млекопитающих посредством гомологичной рекомбинации и векторы для осуществления этого способа
ATE78293T1 (de) Verfahren zur herstellung eines rekombinanten baculovirus-expressionsvektors.
CA2203569A1 (en) Nucleotide sequence encoding the enzyme i-scei and the uses thereof
KR920012442A (ko) 피치아 파스토리스 산성 인산효소 유전자의 dna 단편 및 이를 사용하는 방법
ES8305413A1 (es) Un metodo para estabilizar y seleccionar celulas huesped quecontienen un adn recombinante
DE69328514D1 (de) Herstellung rekombinanter Proteine unter Verwendung von Herpes-Virus-Promotoren und VP16-Transaktivatoren
CA2557882A1 (en) Defective packaging non-oncoviral vectors based on mpmv and hiv
KR870005091A (ko) 인체 라이소자임을 제조하는 방법
HUP0001817A2 (hu) Chlorella víruspromóterek
WO1994013812A3 (en) Novel entomopoxvirus genes, proteins and methods of use thereof
KR940002352A (ko) 디(d)-아미노산 옥시다제 생산성 형질전환체
WO2000015772A3 (en) Recombinant protein production in urine
CA2296813A1 (en) Novel synthetic genes for plant gums
JPH0515380A (ja) ベクター
DK326289D0 (da) Fremgangsmaade til fremstilling af et protein ud fra gaersvampe under anvendelse af et inducerbart system, samt vektorer og transformerede stammer til brug ved fremgangsmaaden
KR880701285A (ko) Aids 비루스의 f단백질을 함유하는 백신
EP1243656A3 (en) Novel baculovirus transfer vectors and recombinant baculoviruses for exogenous gene expression
KR880007728A (ko) 비루스 벡터 및 재조합 dna
Yeung et al. Production of rabbit polyclonal antibody against apobec-1 by genetic immunization
EP0109559A1 (en) High-efficiency yeast vector plasmid and method of its use
KR870006185A (ko) 트롬빈 억제제의 제조방법

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19830813

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 19861016

Comment text: Request for Examination of Application

Patent event code: PA02011R01I

Patent event date: 19830813

Comment text: Patent Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 19880725

Patent event code: PE09021S01D

G160 Decision to publish patent application
PG1605 Publication of application before grant of patent

Comment text: Decision on Publication of Application

Patent event code: PG16051S01I

Patent event date: 19881203

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 19890531

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 19890605

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 19890605

End annual number: 3

Start annual number: 1

PR1001 Payment of annual fee

Payment date: 19920128

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 19930128

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 19940127

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 19950127

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 19960130

Start annual number: 8

End annual number: 8

PR1001 Payment of annual fee

Payment date: 19970129

Start annual number: 9

End annual number: 9

PR1001 Payment of annual fee

Payment date: 19980131

Start annual number: 10

End annual number: 10

PR1001 Payment of annual fee

Payment date: 19990202

Start annual number: 11

End annual number: 11

PR1001 Payment of annual fee

Payment date: 20000129

Start annual number: 12

End annual number: 12

PR1001 Payment of annual fee

Payment date: 20010131

Start annual number: 13

End annual number: 13

PR1001 Payment of annual fee

Payment date: 20020131

Start annual number: 14

End annual number: 14

FPAY Annual fee payment

Payment date: 20030205

Year of fee payment: 15

PR1001 Payment of annual fee

Payment date: 20030205

Start annual number: 15

End annual number: 15

EXPY Expiration of term
PC1801 Expiration of term