KR20170022343A - Prevention, improvement of skin allergy agent based on lactic acid, floral water and medicinal herb extract - Google Patents

Abstract

Disclosed is an agent for preventing and alleviating skin allergy consisting of a lactobacillus fermented solution, floral water and a medicinal herb extract. The lactobacillus fermented solution in the agent is a fermented solution by natural microorganism used for producing foods, and thus is safe. Also, floral water and the medicinal herb extract are extracts of plants which have been used as medicinal herbs or tea for hundreds to thousands of years, thereby having high safety with respect to the skin of the human body and being used as antipruritic agents and antiallergic drugs.

Description

FIELD OF THE INVENTION The present invention relates to a skin allergy prevention and improvement agent based on lactic acid fermentation broth, floral water and herbal extract,

The present invention relates to a prophylactic and / or ameliorative agent for various skin allergies such as pruritus and skin troubles based on lactic acid fermentation broth, floral water and herbal medicine extract.

In recent years, skin pruritus such as food, various pollen of various trees and flowers, house dust, house dust mites, atopic dermatitis caused by chemical substances, skin diseases and allergic symptoms are social problems.

Particularly, with the high airtightness of houses, use of volatile organic compounds such as formaldehyde and toluene at the time of construction, indoor breeding of pets, breeding of house dust mites caused by substances such as skin keratin and dandruff , Continuous use of air conditioning and heating causes house dusts such as ticks, carcasses, powder, mold, pollen, pet hair, and chemical substances such as volatile organic compounds to stay in the room for a long time and cause various allergic symptoms .

In general, antihistamines and antiallergic agents are mainly used for the treatment of pruritus, skin troubles and allergic symptoms. Examples of the external preparations include antihistamines, adrenocorticosteroids, bisteronyl anti-inflammatory agents and opioid κ receptor-activating compounds Although medicines are used, the time required for the internal medicine to take time is long, and side effects such as the depression of the central nervous system such as sleepiness and fatigue after taking the medicine are great, side effects such as digestive system trouble are large, And adverse side effects such as deterioration of adrenal function during long-term use have become a problem.

There is almost no continuous effect in solving the aforementioned side effects and the relief of the above symptoms. Therefore, it is possible to use a liquid or ointment derived from a food which is relatively easily safe, to prevent, ameliorate or treat pruritus, skin trouble or allergy symptoms Is desired.

For example, persimmon leaf extract has been confirmed to be effective in skin troubles and atopic dermatitis models (Patent Document 1). There is not so much known for its effective efficacy for foods that can alleviate allergic symptoms or for highly safe natural microorganisms. However, lactic acid bacteria and their fermentation broths have conventionally been used in the manufacture of foods, and 1,4 -Dihydroxy-2-naphthoic acid (DHNA) has been reported (Patent Document 2), and the use of a culture medium containing proteolytic enzyme-treated products of dairy products in the presence of propionibacterium- There has been known a skin roughness prevention / remedy comprising as an active ingredient a culture solution which has been cultured for 3 weeks (Patent Document 3). However, the symptoms of skin troubles and allergies can not be expected to improve in a short time due to ingestion of food, and the effect on pruritus can hardly be expected.

In order to solve the above-mentioned problem, a number of patent applications related to the use of conventionally used herbal medicine, so-called herbal medicine ingredient, which have relatively few side effects are confirmed. It has been reported that an extract obtained by extracting a natural mixture, such as a white rice flour and a fermented soybean extract with water or an organic solvent and filtering the same, has an antibacterial effect, an anti-inflammatory effect and an improvement in atopic healing (Patent Document 4). According to this patent document, when extracting a herbal medicine ingredient and a fermented soybean extract, there is little difference when extracting with an organic solvent such as water (purified water) or ethanol, and in fermented soybean, how much fermentation has been described It is difficult to know how much the efficacy of the extract is effective, and it is difficult to recognize the degree of improvement in the practical application of 60 days to confirm the improvement effect, and it is difficult to expect that the atopic patient can apply for a long time to obtain the improvement effect It is difficult to say that the composition is satisfactory for consumers, and the fact that the inventions of these patent contents are not realistically can be disproved. Similarly, there is a report that foods prepared by fermenting lactic acid bacteria with herbaceous plants as a component of a nutrient source are effective for cold and atopic dermatitis (Patent Documents 5 and 6). However, in order to ferment plant components together with lactic acid bacteria, saccharides such as oligosaccharides, lactic acid bacteria or yeast are put into a fermentation tank and fermented for 3 days or 30 to 90 days to obtain a fermentation broth, It is difficult to maintain the homogeneity of the final product, and it is considered that the homeostasis of improvement against diseases such as atopy is also unreliable. For this reason, the product of the present invention is not activated in the market .

In addition, there have been reported many external preparations (including cosmetics) for improving skin troubles such as atopic skin containing, as an active ingredient, a mixed extract of ginseng, platinum, plum, lobule, Literature 7 to 23).

Patent Document 1: Japanese Patent Application Laid-Open No. 2000-139406 Patent Document 2: International Publication No. 2003/016544 Pamphlet Patent Document 3: Patent Publication No. 10-2010-0126285 Patent Document 4: Patent No. 10-0431076 Patent Document 5: Patent No. 10-1043509 Patent Document 6: Patent No. 10-1412500 Patent Document 7: Patent No. 10-1447415 Patent Document 8: Patent No. 10-1257435 Patent Document 9: Patent No. 10-1270635 Patent Document 10: Patent No. 10-1252107 Patent Document 11: Patent No. 10-1226758 Patent Document 12: Patent No. 10-1270736 Patent Document 13: Japanese Patent No. 10-1068375 Patent Document 14: Patent No. 10-1087572 Patent Document 15: Patent No. 10-1262813 Patent Document 16: Patent No. 10-0906386 Patent Document 17: Patent No. 10-1013061 Patent Document 18: Patent No. 10-087557 Patent Document 19: Patent No. 10-0875075 Patent Document 20: Patent No. 10-1127393 Patent Document 21: Patent No. 10-0813781 Patent Document 22: Patent No. 10-0769302 Patent Document 23: Japanese Patent No. 10-1108986

It is an object of the present invention to provide a liquid preparation which is high in safety, simple in manufacturing process, can be produced within a short period of time, and exhibits atopy, pruritus, skin troubles and allergies within a short period of time .

The inventors of the present invention have conducted studies on a substance capable of reducing pruritus, skin troubles and allergy symptoms, and found that a mixture of lactic acid fermentation broth, floral water and herbal extract is effective against pruritus or dermatitis caused by insects such as mosquitoes, Dermatitis, atopic symptoms due to various harmful chemicals, and passive skin anaphylaxis (PCA) reactions, and that the above mixed liquid is useful as an anti-allergic agent and as an anti-allergy agent.

Accordingly, the present invention provides an anticancer agent containing as an active ingredient a mixed solution of lactic acid fermentation broth, floral water and herbal medicine extract.

In addition, the present invention provides an antiallergic agent comprising, as an active ingredient, a mixed liquid of lactic acid fermentation broth, floral water and herbal medicine extract.

The lactic acid fermentation broth of the fermented broth of Lactobacillus fermentation broth, floral water and herbal medicine extract of the present invention is a fermentation broth using natural microorganisms conventionally used for food production, so that the safety is high, and the floral water and herbal extracts are used for hundreds to thousands of years Or extracts of plants which have been used as drinking tea, and thus are highly safe against human skin and are useful as anti-allergic agents and as anti-allergic agents. In addition, the fermentation broth can be produced in large quantities to use microorganisms, and it is also possible to mass-produce floral water and herbal medicine extracts, and these fermentation broths and extracts can also be commercially available.

Figs. 1 and 2 are photographs showing the state of the liquid preparation prepared in Preparation Example 1 of the present invention before, during 3 days of application, and 2 weeks after application, to a teenage female student suffering from atopic acne accompanied by severe pruritus.
FIG. 3 is a photograph showing the state of the arm part before application of the 30 th housewife with atopic symptoms, and the state after 3 days of application and 2 weeks after application of the liquid preparation prepared in Production Example 1 of the present invention.
FIG. 4 is a photograph showing the state of a woman in her 40s who had symptoms of atopy on the neck area 2 weeks before application and 2 weeks after application.
FIG. 5 is a photograph showing the state of a male in his thirties with atopic symptoms on the back of his hand before and 2 weeks after application. FIG.
FIG. 6 is a photograph showing the state of a woman in her thirties with atopic symptoms on the face and neck, before application, and 3 days after application and 2 weeks after application.
FIG. 7 is a photograph showing the state of a woman in her 40s with atopic symptoms on the back of her hand and neck, before and 2 weeks after application.
FIG. 8 is a photograph showing the state of a woman in her 30s who has atopic symptoms in the calf and knee folds, before and 3 days after application.

Lactic acid bacteria used in the lactic acid fermentation broth used in the present invention include Lactobacillus sp., Streptococcus sp., Propionibacterium sp., Propionis monacus sp., Propionibacterium sp., Propionimicrobium sp., Propionibacterium sp. , And bacteria belonging to Lactobacillus bacteria, Streptococcus thermophilus, and Lactobacillus lactic are preferable, although not particularly limited thereto.

The nutrient source of the culture medium used in the culturing process of the fermentation broth of lactic acid bacteria in the mixed solution of the present invention is not impossible to use dairy products such as milk and peptone used in the conventional culture process, Stickiness, high viscosity, etc., end users may show a sense of rejection when used as cosmetics or external preparations. Therefore, the components of these dairy products are not used as a culture medium. As the fermentation medium nutrient source used in the present invention, it is preferable to use kelp extract, whey, brown rice, peptone and a small amount of an inorganic nutrient source. These cultured fermented products are obtained by fermentation at 30 to 37 ° C for 6 to 12 hours.

Various known aerobic or anaerobic cultivation methods can be used for culturing the lactic acid bacteria for producing the fermentation broth for use in the present invention. Aerobic or anaerobic cultivation methods using a liquid culture medium are preferable from the viewpoint of mass production.

In the anaerobic culture method, the incubation temperature during cultivation is 20 to 40 캜, preferably 30 to 37 캜. The pH of the medium at this time is preferably adjusted to a neutral to slightly acidic, specifically 5.0 to 8.0, and the culture time is not particularly limited, but is 6 hours to 3 days, preferably 6 hours to 12 hours.

The lactic acid fermentation broth, which is an effective ingredient of the present invention, is obtained by culturing the lactic acid bacteria by the culture method using the above-mentioned culture medium, and includes both of the lactic acid bacteria and the culture. At this time, it is preferable that 1 ml of the obtained fermentation broth contains 1 x 10 ³ cfu to 1 x 10 ⁹ cfu of lactic acid bacteria.

The obtained lactic acid bacteria and the fermentation broth thereof are heated at 70 to 90 DEG C for 7 to 20 minutes to kill the lactic acid bacteria to stop its activity. Failure to do so may result in the continued propagation of lactic acid bacteria in the final or stock solution, resulting in the failure or destruction of the desired final product quality, and furthermore the product being corrupted. The lactic acid bacteria thus obtained may be used as they are, but it is preferred that the fermentation broth containing the killed lactic acid bacteria be pulverized by means of a pulverizer so that particles of solid matter such as lactic acid germs and the like become 50 탆 or less. If necessary, the fermentation broth can be appropriately concentrated or diluted. The thus obtained fermentation broth can be stored in a stock solution at a temperature of 1 to 5 ° C and used appropriately when necessary.

The blending amount of the thus-obtained lactic acid fermentation broth may be 7 to 15 parts by weight, preferably 8 to 12 parts by weight, based on the whole composition. When the content is lower than the lower limit of the above-mentioned content, the effect of improving pruritus and skin troubles is deteriorated. If the content is higher than the upper limit value, the resulting product becomes less sticky to the skin and sticky to the skin, There is a problem that a strong fragrance should be used to solve this problem.

As one of the active ingredients of the present invention, the floral water is extracted by the low-temperature critical extraction method and is also commonly referred to as flowering water. This floral water is the leaves and petals of lavender, Centella asiatica (L.) Urb. Leaf, green tea, calendula petals extracted with purified water. A commercially available vacuum extractor can be used. For example, a vacuum extractor of Chamma Corporation, a COSMOS-660 of Kyusyu Machine, an ultrahigh vacuum low temperature (concentrated) extractor, and an ultrasonic extractor may be used.

Although the extraction method is not particularly limited, when the leaves, petals, roots, etc. of the plant are mixed at a ratio of 1: 1 to 1: 5 to distilled water and extracted at 70 to 80 ° C for 8 to 12 hours, 1 to 3 parts by weight of a vegetable oil. The extract may be extracted for each component or may be mixed at one time, but it is preferable to extract each component to obtain a floral water, and then store it in a stock solution. If necessary, it is decompressed and concentrated at a low temperature to concentrate the active ingredient in the obtained floral water to 30 to 50% by weight with respect to water to prepare a stock solution, which is diluted with water for 5 to 10 minutes It may be used by heating at about 70 to 80 占 폚.

Also, the herbal medicine extract which is one of the effective components of the composition of the present invention means a herb extract, a herb extract, a herb extract, a neem leaf extract and a mash extract. The method of extracting these extracts is not particularly limited, and for example, a conventionally used method, that is, the aforementioned vacuum low-temperature thickening extractor may be used. When the solvent is extracted at 70 to 80 ° C for about 8 to 15 hours using one or more mixed solvents selected from water, ethanol, glycerin, isobutyl alcohol, butylene glycol and other organic solvents, 7% by weight of the extract can be obtained. It is known in the art that such a method can be obtained in a short time at a temperature much lower than the above-mentioned temperature under vacuum and reduced pressure.

If necessary, it is decompressed and concentrated at a low temperature to concentrate the active ingredient in an amount of 30 to 50% by weight based on the total weight of the extract to obtain a stock solution, which is diluted with water for 5 to 10 minutes It may be used by heating at 70 to 80 占 폚.

Of the plant ingredients in the floral water and herbal medicine extracts, lavender flowers have been known to be effective for treating various kinds of pain such as headache, toothache, menstrual pain, acne and the like, and it is widely known as a main component of external preparations such as ointment for wound healing It is known that yukchepa has the therapeutic effect on anticancer effect, natural antibiotic, chronic rhinitis and atopy (Kukmin Ilbo 2013, 6, 10), and Chungho is useful for various trauma, gastritis, blood circulation and skin diseases. And is widely used in natural soaps and aromatherapy. Licorice contains a large amount of natural steroids. It is widely known as herbal medicine because of bronchial asthma, skin diseases and whitening.

In the present invention, each of the floral water and herbal medicine extract is blended in an amount of 25 to 45% by weight. If it is mixed below the lower limit of these components, the viscosity of the formulation increases, the effect on the pruritus deteriorates, the spreadability to the skin becomes worse, and the skin becomes heavy. When the upper limit is exceeded, have.

The skin improving agent of the present invention is preferably blended with a moisturizing agent to improve the moisturizing effect. As the moisturizing agent, hyaluronic acid salts such as sodium hyaluronate, ceramide, carnauba wax and the like which are generally used in the art can be formulated. The blending amount thereof may be about 1 to 10% by weight based on the whole amount.

When the viscosity is low, about 0.5 to 2% by weight of gum arabic, xanthan gum, cellulose or the like may be added to improve the viscosity.

In addition, an antioxidant can be added to the skin improving agent of the present invention to prolong the shelf life thereof. As the antioxidant, ascorbic acid or its salt, ascorbic acid ester, erythorbic acid and the like are used, but ascorbic acid or its salt is particularly preferable. Examples of the salt include alkali metal salts such as sodium and potassium; alkaline earth metal salts such as calcium and magnesium; and ammonia salts.

The content of the antioxidant is preferably from 0.01 to 3% by weight based on the whole amount.

As shown in the later test examples, the skin improving agent containing the lactic acid fermentation broth, the floral water and the herbal extract of the present invention increases the water content and skin score of each layer. Therefore, the agent for improving the dry skin condition, that is, useful. As one of the methods for treating atopic dermatitis, there is a method of moisturizing dry skin and improving the skin condition, so that the agent for preventing or improving skin roughness is useful for the treatment of atopic dermatitis, It can also be used as an anti-allergic agent. Here, " rough skin " means that the water content of each layer decreases and the surface of the skin becomes dry or rough skin becomes rough.

In addition, although the skin improving agent of the present invention is described later as a liquid preparation, it can be a conventional ointment agent or other formulation, and is not limited to these agents.

Hereinafter, an example of the prevention and improvement of the skin allergy of the present invention will be described.

Example

Hereinafter, the present invention will be described in detail with reference to examples. Means " parts ", "% ", " parts "

1) Preparation of lactic acid fermentation broth, floral water and extract:

(1) Preparation of lactic acid fermentation broth

Lactobacillus (lactobacillus bacteria) was inoculated thereinto and cultured at 35 to 38 占 폚 for 48 hours at a temperature of 35 to 38 占 폚, And fermented to obtain a lactic acid fermentation broth. This was ground with a mixer and stored in a refrigerator (about 3 ° C).

(2) Preparation of floral water

Lavender floral water was obtained by adding dry lavender flowers, leaves and distilled water at a weight ratio of 1: 1 to a high-speed vacuum low-temperature extracting machine (Kyosho Machine, COSMOS-660) at about 70 to 80 ° C for 8 to 12 hours. This floral water was again added to the vacuum low-temperature extractor and concentrated so that the lavender plant extract component was 30 to 50 wt%. The mixture was allowed to cool and stored at about 3 ° C.

Bottle grass, green tea leaves, and calendula leaves were prepared in the same manner to prepare each of the floral water.

(3) Preparation of extract

In a high-speed vacuum low-temperature extractor (Kyosho Machine, COSMOS-660), the dried plantlets were placed in a solvent of butylene glycol / ethanol / distilled water (50/25/25) at a ratio of 1: 5, The extracts were obtained by extracting time - The obtained extract was distilled and the organic solvent was recovered. The concentration of the extract was about 5%. The extract was concentrated again to remove the water content of the extract by about 50%. The thus-obtained Centella asiatica extract was stored in a refrigerator (about 3 ° C).

The extracts were prepared in the same manner for each of Chungho (zygoma), yuguni, maji and mysterious leaves.

Production Example 1: Preparation of a solution for improving skin trouble of the present invention

10 g of the lactic acid bacteria fermentation broth was placed in a beaker, and 10 g of green tea floral water, 10 g of lavender floral water, 12 g of bottled plant leaf water and 8 g of calendula floral water were added to the beaker and then heated to about 70 캜. After heating, the mixture was homogenized by homogenizer for about 10 minutes.

8 g of each of the centilla extract, chrysophyllum extract, mulberry leaf extract, root extract, and mashii extract was added to a separate beaker and heated to 80 ° C. The thus obtained extract mixture solution was added to the mixture of the lactic acid fermentation broth and the floral water and stirred for about 10 minutes with a homogenizer to obtain a homogeneous solution.

Test Example 1: Inhibitory test for atopic dermatitis

(1) Outline

The applicants suffering from atopic dermatitis were allowed to apply the liquid preparation of Example 1 three times a day for one week, and the results were confirmed.

(2) Results

All volunteers who applied the liquid preparation of Preparation Example 1 answered that the pruritus completely disappeared on the first day of application and all of them were improved on the 3rd day after application, and after 14 days of application, almost all of the atopic symptoms were removed, Respectively. The photographs before application and those after 7 days of application are shown in Figs. 1 to 7.

In these figures, Figs. 1 and 2 show the state of the liquid preparation prepared in Preparation Example 1 of the present invention before application to 10-year-old female students with atopic acne symptoms accompanied by severe pruritus, 3 days after application and 2 weeks after application As a photograph, pruritus has disappeared from the third day of application and almost all of the acne-causing atopy has disappeared in the second week of application.

Fig. 3 is a photograph showing the state of the arm part before application of the 30th housewife with atopic symptoms and the state after 3 days of application and 2 weeks after application of the liquid preparation manufactured in the production example 1 of the present invention. , And pruritus disappeared, and almost all of the atopy disappeared after 2 weeks of application.

FIG. 4 is a photograph showing the state of a woman in her 40s who has symptoms of atopy on the neck area after application and after 2 weeks of application, showing that atopy almost disappears after 2 weeks of application.

FIG. 5 is a photograph showing the state of a male in his thirties with a symptom of atopy in the back of his hand before and 2 weeks after application, showing that atopy almost completely disappeared after two weeks of application.

FIG. 6 is a photograph showing the condition of a woman in her thirties with atopic symptoms on the face and neck after 3 days of application and 2 weeks after application. In the first day of application, pruritus disappeared and there was no inconvenience in life. Later, symptoms of atopy almost disappeared.

FIG. 7 is a photograph showing the state of a woman in her 40s who has symptoms of atopy on the back of the hand and neck after 2 weeks of application, showing that atopy almost disappears after 2 weeks of application.

FIG. 8 is a photograph showing a state of a woman in her thirties with a symptom of atopy in the calf and a knee folded part before and 3 days after application. In the third day of application, pruritus disappears and atopic symptoms are significantly improved.

Claims (4)

Lactobacillus fermentation broth obtained by using lactobacillus belonging to the genus Lactobacillus, Streptococcus, and Propionibacterium in a medium comprising brown rice, kelp extract and whey;
Floral water extracted from distilled water with plants consisting of lavender petal, cinnabar, green tea, and calendula; And
The present invention relates to a method for alleviating or alleviating skin troubles, which is obtained by warming the extracts of yugi, ciliate, cinnabar, feminized mint leaf, and maji prefecture at 50 to 80 캜 and stirring with a homogenizer. The improvement according to claim 1, wherein the lactic acid bacterium is Lactobacillus bacteria, Streptococcus thermophilus and Lactobacillus lactic. The improvement or alleviation of skin trouble as claimed in claim 1 or 2, wherein the lactic acid fermentation broth: floral water: herbal medicine extract is blended in a ratio of 7:15 to 25:45 to 45:25. The relaxation and remedy for skin trouble as claimed in claim 1 or 2, which is obtained by blending 80 to 120 parts by weight of one kind selected from glycerin, hyaluronic acid salt and ceramide against floral water.

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