KR20110004603A - Cauliflower extract for treating or preventing menopausal diseases, preventing skin aging, or improving skin wrinkles - Google Patents

Abstract

The present invention relates to a composition comprising a painting tree extract that promotes estrogen secretion and inhibits collagenase activity as an active ingredient, and more specifically, includes a painting tree extract as an active ingredient and promotes estrogen secretion by menopause. A composition for treating or preventing a disease, a cosmetic composition for preventing skin aging that contains collagen extract as an active ingredient, and a collagen extract for inhibiting collagenase activity, and a wrinkle that contains collagena extract as an active ingredient, and inhibits collagenase activity It relates to a cosmetic composition for improvement.

The composition comprising the extract of the present invention as an active ingredient promotes estrogen secretion, is more effective than estradiol among the estrogen replacement agents used in conventional hormone replacement therapy, and at the same time inhibits collagenase activity, thereby preventing menopausal diseases At the same time it can be treated or prevented, it can also be used for preventing skin aging or improving skin wrinkles.

Therefore, the extract of the present invention can be applied to various symptoms of women after menopause lacking the secretion of estrogen, and at the same time, the skin improvement effect can also be used to improve, prevent, or treat the symptoms after menopause.

 Painting tree, gangrene, estrogen, menopause, menopause, phytoestrogens, osteoporosis, hot flashes, anti-aging skin, wrinkle improvement

Description

Extracts from Sophora Japonica L. for Treating or Preventing Menopausal Complaints, Skin-Aging, or Skin Wrinkle}

The present invention relates to a composition comprising, as an active ingredient, a painting tree extract that can be used for the treatment or prevention of menopausal diseases, to prevent skin aging, or to improve skin wrinkles.

Estrogen is a female hormone synthesized from cholesterol in the body and secreted by the ovaries, adrenal cortex, testes, and fetal placenta, estradiol, estrone, and estriol. Estradiol is known to be the most useful.

These estrogens are excellent in protecting the functions of various tissues such as the heart, blood vessels, and central nervous system, as well as bone tissues, and maintain the secondary sexual characteristics of women. Embryonic development, cell growth, reproduction, etc. Also plays an important role. In estrus and primates, animals also play a role in promoting the growth of the endometrium during menstruation.

Therefore, when a woman is menopausal and estrogen secretion decreases, various diseases such as facial flushing, neurovascular system such as sweating, motor organ system such as osteoporosis, mental nervous system system such as depression, skin tissue change, and hair loss may occur. Can be.

Therefore, the method of preventing the disease of women after menopause by administering the above-mentioned estrogen is widely used. However, when taking estrogen preparations for a long time, there have been problems of serious side effects such as cancer and gallbladder disease. Therefore, the current hormone replacement method is used as a treatment method for such menopausal diseases, and among them, the role of phytoestrogen is dealt heavily.

The phytoestrogens are estrogen mimetics that exhibit estrogen-generating effects and bind to estrogen receptors in cells to recognize and bind to specific nucleotide sequences, ie, estrogen responsive elements, of DNA. The combination of these genes and proteins increases the transcription of certain proteins, and is known to induce cellular responses by estrogen analogs. (Gaido KW et al. Endocrinology 1999 140 (12): 5746-53; Gronen S et al., Environ. Health Perspect. 1999 107 (5): 385-90)

The estrogen replacement effect, while having the effects of inhibiting cancer cells, relieving menopausal disorder symptoms, anti-aging, etc., it is polite to find and use safer phytoestrogens that are separated from plants.

The inventors of the present invention have completed the present invention while repeatedly researching an extract containing phytoestrogens as an estrogen replacement agent.

Accordingly, the present invention provides a composition comprising a painting extract as an active ingredient to promote estrogen secretion, inhibiting collagenase activity.

In order to achieve the above object, the present invention includes a painting tree extract as an active ingredient, and provides a treatment or prevention composition for menopausal diseases promoting estrogen secretion.

In another aspect, the present invention comprises a painting extract as an active ingredient, and provides a cosmetic composition for preventing skin aging and a cosmetic composition for improving skin wrinkles that inhibit collagenase activity.

The present invention provides a therapeutic or prophylactic composition for postmenopausal disease, comprising a cedar extract as an active ingredient and promoting estrogen secretion.

The painting tree is a scientific name Sophora japonica, belongs to the legumes, 25m in height and around, and is distributed in Korea, Japan, China and the like. Branches are spread and small branches are green and have a smell when they are cut. Leaves are alternate and single pinnate leaves. Small leaves 1 to 17 each, egg-shaped or oval-shaped oval, with petioles on the back, with lie down hairs, flowers bloom in light yellow in August and run in conical inflorescences. The pod has a length of 5 to 8 cm and has a drooping shape when the seeds are inserted in a narrow shape.

The buds of the painting tree as described above are called Goehwa or Goemi, and the fruit is called Goesil or Goke. Fruits are citrus, columnar or beads. Fruits are used to treat hemorrhoids, along with branches and bark. Garden trees and wood are also used as furniture. On the other hand, the known use of hardening is the treatment or prevention of atherosclerosis and high stenosis, and it is also used to make beer and paper yellow.

The inventors of the present invention, while repeatedly researching phytoestrogens as an estrogen replacement agent, found that the fruit of the above-mentioned picture tree, especially the picture tree, promotes estrogen secretion and inhibits collagenase synthesis, thus completing the present invention. It was. In particular, the experimental results have been found that the estrogen secretion activity of the painting tree fruit extract of the present application is better than the estradiol known to be the most effective of the estrogens to complete the present invention. In addition, in the case of the composition containing the extract as an active ingredient, the effect of replacing estrogen, while suppressing cancer cells, alleviating symptoms of menopausal disorders, and preventing aging, while being separated from the plant, it is safer as a safe phytoestrogens. It is anticipated that it can be used widely, as a therapeutic or prophylactic composition for menopausal diseases.

At this time, if the painting tree extract is derived from the painting tree, it can be used to extract without limitation of the constitution, Preferably, the lump extract which is a fruit extract is preferable. Among the various extracts from the extract, the fruit extract, the gangwi extract, was found to be the most effective in inhibiting estrogen secretion and collagenase activity.

On the other hand, the extraction solvent used for the preparation of the extract as described above may be selected without limiting its configuration. Specifically, it may be extracted with at least one extraction solvent selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, ethyl acetate, hexane, dichloromethane, acefon, chloroform, methylene chloride, and mixtures thereof. At this time, the alcohol having 1 to 5 carbon atoms can be selected and used without limitation of the composition, preferably ethanol can be used, more preferably extracted using 75 to 85% (v / v) of ethanol can do. The estrogen secretion activity of the cedar extract extracted using 75 to 85% (v / v) of ethanol as described above was confirmed to be superior to estradiol, as described in the results of the experimental examples described below. Most preferably it can be extracted using 80% ethanol (v / v).

On the other hand, the painting tree extract as described above may be prepared according to the conventional method for producing a plant extract. Preferably the flower, fruit, bark, roots or roots of the painting tree is pulverized, and then the residue is removed. Then, an extraction solvent corresponding to 3 to 7 times the weight ratio of the pulverized product is added thereto, and room temperature. Can be extracted from

On the other hand, the extraction time is not limited, but preferably 10 to 24 hours extraction. If the extraction time is less than 10 hours, the content of the active ingredient to be extracted is small, if more than 24 hours, the increase in efficacy as the extraction time is long is not preferable in terms of productivity.

The picture extract extracted in the above manner is filtered through a filter cloth or the like, the filtrate is centrifuged to remove the precipitate, and then concentrated under reduced pressure or concentrated, and then freeze-dried. More preferably, the composition may be extracted from the painting tree fruit with 75 to 85% (v / v) of ethanol at room temperature for 10 to 24 hours. Most preferably, the composition may be extracted from the painting tree fruit with 80% (v / v) of ethanol at room temperature for 10 to 24 hours.

The effect of inhibiting estrogen secretion promoting activity and collagenase activity of the extract of the Prunus japonica extract extracted under the above conditions was observed.

Meanwhile, the term "menopausal disease" as used herein refers to a disease that is associated with decreased estrogen secretion in postmenopausal women, and cardiovascular diseases such as atherosclerotic cardiovascular disease, postmenopausal cardiac disease, hot flashes, and sweating. Abnormalities, motor organ abnormalities such as osteoporosis, mental nervous system abnormalities such as depression, skin tissue changes and alopecia and the like.

On the other hand, the content of the painting tree extract as an active ingredient in the treatment or prevention composition for menopausal diseases of the above-described embodiment is not limited, but may preferably be 0.001 to 30% by weight. When the content of the extract of the active tree is less than 0.001% by weight, the effect of promoting estrogen secretion or inhibiting collagenase activity is insignificant, and when it exceeds 30% by weight, the effect of increasing the activity by increasing the content is insignificant. Not

The present invention provides a medicament comprising the extract according to the embodiment as an active ingredient, comprising a therapeutic or prophylactic composition for menopausal diseases promoting estrogen secretion. Medicines containing the painting extract as an active ingredient may further comprise a suitable carrier, excipients and diluents commonly used in the preparation thereof.

Carriers, excipients and diluents that may be included in a pharmaceutical product containing the extract of the present invention as an active ingredient include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

Medicines containing the extract of the present invention as an active ingredient may be used in oral formulations, such as powders, granules, tablets, suspensions, emulsions, syrups, etc. according to conventional methods.

The oral formulation is meant to include a solid preparation and a liquid preparation for oral administration, the solid preparation for oral administration may include tablets, pills, powders, granules, capsules, etc., such solid preparation is the extract At least one excipient, for example, may be prepared by mixing starch, calcium carbonate, sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solvents, emulsions, and serums.In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. have.

Preferred dosages of the compositions containing the cedar extract of the present invention vary depending on the condition and body weight, extent of disease, drug form, route of administration and duration, and may be appropriately selected by those skilled in the art. Preferably, the medicine containing the painting tree extract of the present invention as an active ingredient is 0.0001 to 100mg / kg per day based on the amount of the painting tree extract, in order to be more effective it is preferably administered at 0.01 to 10mg / kg. Administration may be administered once a day or may be divided several times. The dosage and frequency of administration are not intended to limit the scope of the invention in any aspect.

According to another embodiment of the present invention, there is provided a functional food for the improvement or prevention of menopausal diseases comprising a painting extract as an active ingredient. As used herein, the term "health functional food" means a natural product or a processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through a certain processing process.

Examples of health functional foods to which the painting extract of the present invention can be added include various foods, beverages, gums, teas, vitamin complexes, and the like. In addition, the food in the present invention includes special nutritional products (e.g., prepared oils, infants, baby food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), health supplements, seasoned foods ( For example, soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi, pickles, etc.), beverages ( Examples include, but are not limited to, fruits, vegetable drinks, soy milk, fermented beverages, and the like, and natural seasonings (eg, ramen soup). The food, beverage or food additives may be prepared by a conventional manufacturing method.

In the present specification, the functional food is a biological defense rhythm control, disease prevention and the like having a food group or food composition that has added value to the food by using physical, biochemical, biotechnological techniques, etc. to function and express the function of the food for a specific purpose. It means a food that is designed and processed to fully express the gymnastics function on recovery. The functional food may include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the manufacture of functional foods.

In the present specification, the beverage is a generic term for drinking to quench thirst or to enjoy a taste and is intended to include a functional beverage. The beverage is not particularly limited to other ingredients other than including the painting tree extract as an active ingredient in the indicated ratio as an active ingredient, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. . Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate may generally be about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention. It may further contain a pulp for.

In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. These components can be used independently or in combination. The proportion of such additives is not so critical, but may be selected in the range of 0 to 20 parts by weight per 100 parts by weight of the extract of the present invention.

In the present specification, the functional beverage refers to a biological defense rhythm control, disease prevention, and the like having a beverage group or a beverage composition which has added value to the beverage by using physical, biochemical, or biotechnological techniques to act and express the function of the beverage to a specific purpose. Means a beverage that is designed and processed to fully express the gymnastics function related to recovery.

The functional beverage is not particularly limited to other ingredients except the painting tree extract of the present invention as an essential ingredient at the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and xylitol Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.

In addition, in the health functional food for the purpose of improving or preventing menopausal diseases, the amount of the extract may include 0.01 to 15% by weight of the total food weight, the beverage composition is 0.02 to 5 g based on 100 ml, Preferably it may be included in a ratio of 0.3 to 1g.

According to another embodiment, the present invention provides a cosmetic composition for preventing skin aging, which comprises a painting extract as an active ingredient and inhibits collagenase activity.

In addition, the present invention provides a cosmetic composition for improving skin wrinkles, which comprises a painting tree extract as an active ingredient and inhibits collagenase activity according to another embodiment.

The cosmetic composition for preventing skin aging or improving skin wrinkles according to the embodiments as described above comprises a painting extract as an active ingredient, inhibiting the activity of collagenase, a degradation enzyme of collagen, which is a major component of the skin, It can be usefully used for anti-aging or for improving skin wrinkles.

As mentioned in the above-described embodiment of the treatment or preventive composition for treating menopausal diseases, which comprises the extract as an active ingredient and promotes estrogen secretion, in the cosmetic composition for preventing skin aging or the cosmetic composition for improving skin wrinkles, The tree extract is preferably an extract of the ash tree fruit.

A composition for preventing skin aging or for improving skin wrinkles, which comprises the above picture extract as an active ingredient, may be prepared according to the method mentioned in the treatment or prevention composition for menopausal diseases described above.

Preferably, the extract may be extracted with at least one extractant selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, ethyl acetate, hexane, dichloromethane, acefon, chloroform, methylene chloride, and mixtures thereof. More preferably, ethanol may be used as the extraction solvent, and most preferably ethanol may use 75 to 85% (v / v) ethanol as the extraction solvent.

The cosmetic composition for preventing skin aging or improving skin wrinkles may be a composition extracted from a tree fruit of 75 to 85% (v / v) of ethanol for 10 to 24 hours at room temperature. More preferably, the extract may be a composition extracted 10 to 24 hours at room temperature with a solvent of 80% (v / v) ethanol.

On the other hand, the skin anti-aging cosmetic composition or cosmetic composition for improving skin wrinkles may be provided in a formulation such as skin anti-aging cosmetics or cosmetics for improving skin wrinkles. Formulations of the above-mentioned cosmetics include any one selected from the group consisting of soft and nourishing cream, nourishing cream, massage cream, essence, foundation, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cream It may be a formulation, but is not limited to the examples described above.

In preparing the formulations of the cosmetic composition comprising the extract as an active ingredient as an active ingredient, 0.001 to 30 parts by weight, preferably 0.01 to 10 parts by weight of the extract of the invention of the present invention in 100 parts by weight of the external preparation composition for skin Parts by weight may be added. On the other hand, in the formulation of the cosmetic composition described above, in addition to the extract of the tree of the present invention, fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances For surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients commonly used in external preparations. In addition, the ingredients may be introduced in amounts generally used in the field of dermatology.

The composition comprising the cedar extract of the present invention as an active ingredient promotes estrogen secretion, has a better effect of promoting estrogen secretion in the body than estradiol among estrogen preparations used in conventional hormone therapy, and simultaneously inhibits collagenase activity, In addition to treating or preventing menopausal diseases, it may be usefully used for the purpose of preventing skin aging or improving skin wrinkles.

Hereinafter, the configuration and effect of the present invention through the specific embodiments of the present invention will be described in more detail. However, the following examples are only intended to more clearly understand the invention, the scope of the invention is not limited to the following examples. Hereinafter, the present invention will be described in detail through examples of the present invention.

[ Experimental Example ]

1. Experimental Effect of Estrogen Secretion Activity of Panax Fruit Extract

In order to test whether the extract of the present invention is effective for estrogen secretion, a transformed cell line comprising an estrogen response sequence to which a labeled gene is bound was prepared, and the sample was treated to measure the expression of the labeled gene. Method was used.

If the specific substance of the sample is bound to the estrogen reaction sequence and the transcription of the specific protein is actively performed, the expression of the marker gene will also increase, so after a certain time after the sample processing, the amount of the expressed marker gene is measured, It can be seen how the sample has acted on the estrogen response sequence. Specific experimental preparations and results are as follows.

1-1) Cell Line Preparation

Gene recombination methods were used to construct genes in which luciferase expression is regulated by each cis-element in a vector.

Estrogen Responsive Element-Luciferase Vector (pcDNA) prepared as described above was transfected into MCF-7 cells, which are breast cell lines using lipopectamine, and then antibiotics (G418). ) Colonies were selected, treated with estradiol, and colonies showing the greatest luciferase activity compared to the control group were used for the experiment.

1-2) Plant Extract Preparation

1-2-1) Painting Tree Fruit Eccentric Preparation of Extract

After crushing 1 kg of Chinese painting tree fruit (Sophora japonica L.) uniformly into 60 mesh, 80% (v / v) alcohol corresponding to 5 times the weight of the painting tree fruit was added and extracted at room temperature for 12 hours. . After the extract was filtered with a filter cloth, the filtrate was centrifuged to remove the precipitate, and then concentrated under reduced pressure and lyophilized.

1-2-2) Country  Preparation of Extract

After putting dried Korean ginseng (Chrysanthemum indicum L.) outpost into purified water corresponding to 5 ~ 10 times the weight of the sample, hot water was extracted at 70 ℃ for 2 hours so that the active substance could be eluted, and then the extract was filtered with a filter cloth. The filtrate was centrifuged to remove the precipitate. The separated extract was concentrated under reduced pressure and freeze dried.

1-2-3) Preparation of Cornus Extract

After drying dried cornus (Korean Cornus officinalis Siebold et Zucc) fruit from Korea in purified water equivalent to 5-10 times the weight of the sample, the hot water was extracted for 2 hours at 70 ℃ or higher to elute the active substance, and the extract was filtered with a filter cloth. The filtrate was then centrifuged to remove the precipitate. The separated extract was concentrated under reduced pressure and freeze dried.

2-1) administering plant extracts to cell lines and Luciferase  Active measurement

Dispense the cell line expressing the labeling gene in a 96-well plate with 5 × 10 ³ cells / 100 μl and negative control group (Dimethyl sulfoxide; DMSO 99% 0.5 μl), the respective plant extracts, the positive control group (estradiol) Estradiol), washed twice with PBS buffer, and 50 μl of lysis buffer (Lysis buffer: 20 mM-Tris, 150 mM NaCl, 1% TX-100, 2 mM DTT) was added to each well of 96-well. Thus, cell lysate was produced.

After 30 minutes of vortexing at 4 ° C. with a multi-vortex, 5 μl of each was transferred to a clinplate, and 25 μl of luciferin was added to measure luciferase activity using a luminometer. It was.

According to the amount of luciferase expressed, the expression level of the estrogen response factor was known. The results are shown in Table 1 and FIG.

RLU
(Relative Light Unit) Drainage
(fold) DMSO 0.0557 One Oyster Extract 5 μg / ml 0.3122 5.6 Oyster Extract 50μg / ml 0.7875 14.1 Oyster Extract 100μg / ml 0.7037 12.6 Estradiol 0.01 nM 0.0947 1.7 Estradiol 0.1 nM 0.3205 5.6

As can be seen in Table 1, the worm extract (Panax fruit extract) showed more than five times the estrogen reaction activity compared to DMSO (dimethyl sulfoxide), a negative control, it was found that even compared to estradiol. Therefore, it is expected that the extract according to the present invention acts as a natural substitute for estrogen, and can be usefully applied to improve and treat various diseases caused by decreased estrogen secretion such as menopausal disorder or aging.

2. Collagenase  Activity inhibitory effect evaluation experiment

1mg of azocol collagen (Sigma) and 9.4units of collagenase enzyme (Sigma) were added to 0.1ml of the extract of the locust fruit, and the total volume was 1ml, and the reaction was carried out at 43 ° C for 1 hour, followed by 10 at 3,000rpm. The supernatant was collected by centrifugation for a minute and then the absorbance was measured at 550 nm. The results are as shown in Table 2 and FIG.

Collagenase Activity (%) Control Collagen + Collagenase 100 Positive control Collagen + Collagenase + Doxycycline 0.25% 24.5
Experimental group Collagen + Collagenase + Nucleus Extract0.5mg 96.3 Collagen + Collagenase + Nucleus Extract 5mg 84.0 Collagen + Collagenase + Nucleus Extract 25mg 65.4

 From the results of Table 2, it can be seen that the wormwood (Pear Tree Fruit) extract has an effect of inhibiting collagen degrading enzyme activity.

3. Estrogen Secretion Efficacy in Animal Experiment In vivo )

The lump extract, persimmon extract, and cornus extract prepared in Experimental Example 1 were prepared in a 2000 mg tablet form with the composition of Table 3 below.

Nerd extract
(wt%) Persimmon Extract
(wt%) Cornus Extract
(wt%) Xylitol
(wt%) Zinc oxide
(wt%) Vitamin c
(wt%) Vitamin B2
(wt%) Dry yeast selenium
(wt%) Tea powder
(wt%) Chicory White
(wt%)
room
city
Yes One 0.001 0 0 15 2 5 One 2 5 69.999 2 0.05 0 0 15 2 5 One 2 5 69.95 3 One 0 0 15 2 5 One 2 5 69 4 One 0.1 0.1 15 2 5 One 2 5 68.8 5 One One One 15 2 5 One 2 5 67 Comparative example 0 0 0 15 2 5 One 2 5 70

The 21-day-old immature Sprague-Dawley female rats were divided into 5 rats per group, and the compositions were orally administered at 200 mg / kg doses for 3 days.

17 Beta-estradiol concentration (pg / ml) Control 108.22 Comparative example 119.87 Example 1 205.21 Example 2 231.87 Example 3 237.96 Example 4 228.46 Example 5 223.92

From the results of Table 4, it can be seen that the composition of the present invention has an estradiol secretion promoting effect.

4. Evaluation of menopausal symptoms improvement efficacy by women

30 women aged 48 years old with menopausal symptoms were divided into 10 people and taken the tablets of Examples 3 and 5 twice a day, once a capsule once a week for 8 weeks, hot flashes, fatigue, insomnia, We evaluated whether depression was improved.

Symptom average after taking Comparative example 0.4 Example 3 2.3 Example 5 2.6

Rating scale

0 points: no effect

1 point: slightly effective

2 points: Effective

3 points: excellent effect

From the results in Table 5, it can be seen that the composition containing the extract of the present invention as an active ingredient is effective in improving female menopausal symptoms.

 On the other hand, the following describes an example of the formulation using the painting tree extract of the present invention, the formulation of the present invention is not limited thereto.

[ Production Example  : Formulation Using Painting Tree Extract]

Production Example  One. Powder  Produce

Panax Extract Powder 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

Production Example  2. Preparation of Tablets

Panax Extract Powder 10 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components was prepared by tableting according to the conventional manufacturing method of the tablet.

Production Example  3. Preparation of Capsule

Panax Extract Powder 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients were mixed and filled into gelatin capsules to prepare capsules.

Production Example  4. Liquid  Produce

Panax Extract Powder 20 mg

10 g of isomerized sugar

5 g of mannitol

Purified water quantity

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution was prepared by sterilization.

Production Example  5. Manufacture of Healthy Foods

Panax Extract Powder 1000mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Production Example  6. Preparation of Softening and Nutritious Cosmetics

Panax Extract Powder 100.0 mg

1, 3-butylene glycol 10.0 mg

Disodium IDT 0.5 mg

Allantoin 1.0 mg

Dipotassium glycylizate 0.5 mg

Citric acid0.1 mg

Sodium citrate 0.2 mg

Glyceres-26 10.0 mg

Arbutin 20.0 mg

Hydrogenated Castor Oil 10.0 mg

Ethanol 300.0 mg

Preservative Trace

Trace amount of colorant

Trace amount of flavor

500 mg of purified water

Production Example  7. Preparation of nutritional cream

Passionfruit Extract 100.0 mg

1, 3-butylene glycol 70.0 mg

Glycerin 10.0 mg

D-panthenol 1.0 mg

Magnesium Aluminum Silicate 3 mg

Jojoba oil 50mg

Liquid Paraffin 70mg

Squalane 30mg

Triethanolamine 3mg

1 is a graph showing a comparison of the results of the estrogen secretion promoting experiments according to the concentration of the positive control group (estradiol) and the present invention.

Figure 2 is a graph showing the collagenase activity inhibitory effect of the lump extract according to an embodiment of the present invention compared to doxycycline.

Claims (20)

A therapeutic or prophylactic composition for treating menopausal diseases, which comprises a painting extract as an active ingredient and promotes estrogen secretion. The composition of claim 1, wherein the painting tree extract is a fruit extract of painting tree. The method of claim 1, The extract is water, alcohol having 1 to 5 carbon atoms, ethyl acetate, hexane, dichloromethane, acephone, chloroform, methylene chloride, and a mixture thereof. The composition of claim 3, wherein the extractant is ethanol. The composition of claim 4, wherein the ethanol is 75-85% (v / v) ethanol. The method of claim 1, The painting tree extract is 75 to 85% (v / v) ethanol extract of the painting tree is a composition that is extracted at room temperature for 10 to 24 hours. The method of claim 1, Composition comprising 0.001 to 30% by weight of the painting tree extract. The method of claim 1, The menopausal disease is osteoporosis, hot flashes, atherosclerotic cardiovascular disease, depression, sweating, or alopecia. Pharmaceuticals for the treatment or prevention of menopausal diseases, including the extract of cedar as an active ingredient, promoting the release of estrogen. The pharmaceutical product of claim 9, wherein the medicine is a powder, granules, tablets, suspensions, emulsions, or syrups. Functional food for the improvement or prevention of menopausal diseases, including the extract as an active ingredient. 12. The health functional food for improving or preventing menopausal diseases according to claim 11, wherein the health functional food is a food, a food additive or a beverage. A cosmetic composition for preventing skin aging, comprising a painting tree extract as an active ingredient and inhibiting collagenase activity. A cosmetic composition for improving skin wrinkles, which comprises a painting tree extract as an active ingredient and inhibits collagenase activity. 15. The composition according to claim 13 or 14, wherein the painting tree extract is a fruit extract of painting tree. The method according to claim 13 or 14, The extract is water, alcohol having 1 to 5 carbon atoms, ethyl acetate, hexane, dichloromethane, acephone, chloroform, methylene chloride, and a mixture thereof. The composition of claim 16, wherein the extractant is ethanol. 18. The composition of claim 17, wherein the ethanol is 75-85% (v / v) ethanol. The method according to claim 13 or 14, The painting tree extract is a composition that extracts the painting fruit from 10 to 24 hours at room temperature with a solvent of 75 to 85% (v / v) of ethanol. The method according to claim 13 or 14, The cosmetic composition has a formulation of any one selected from the group consisting of soft and nourishing longevity, nutrition cream, massage cream, essence, foundation, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cream Cosmetic composition.

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