KR20070113330A - External skin composition comprising gompi extract - Google Patents

Abstract

The present invention relates to the use of gompi extract and ecol, diecol extracted with water, alcohol or a mixture thereof, and more particularly, the transcription factors n-kappabi (NF-kB) and api-1 (AP) -1) by inhibiting type I collagenase, which is a degrading enzyme of the skin matrix, and increasing collagen, which is a major component of skin, has the effect of inhibiting skin inflammation, improving wrinkles, and improving elasticity. It can be used as an external preparation, and can be usefully used without side effects in food and pharmaceutical.

Description

A composition containing Ecklonia stolonifera extract for skin external application}

1 is a diagram showing the inhibitory effect on the transcription factors NF-kB and AP-1 of the Gompi extract, FIG. 2 is a view showing the inhibitory effect on MMP-1 biosynthesis of Gompi extract, Ecole, dietol, Figure 3 Is a diagram showing the inhibitory effect on the MMP-1 biosynthesis of Ecole, Diecol in Western blot results, Figure 4 is a view showing the inhibitory effect on MMP-1 biosynthesis by ultraviolet rays of Gompi extract, Ecole, Diecol, 5 is a view showing the effect of promoting the collagen biosynthesis of Gompi extract, Ecole, diecol, Figure 6 is a diagram showing the effect of inhibiting collagen degradation by alpha necrosis (TNF α) to be.

The present invention relates to an external composition for skin containing Gompi extract and Ecole and Diechol. More specifically, the present invention relates to extracts obtained by extracting the seaweed gomphy, the seaweed of kelp, from an appropriate solvent formulation, and to external preparations containing ethanol and diecol. The present invention can be prepared in the form having a flexible cosmetic water, nourishing cosmetics, massage cream, nourishing cream, pack, gel, essence or skin adhesive type cosmetics, to provide wrinkle improvement, elasticity improvement effect on the skin. The clinical classification of skin aging is natural aging that causes skin to become weaker, pale, finer wrinkles and less elasticity as it ages naturally, and deep and rough wrinkles are caused by sunlight, causing pigmentation and capillary dilatation. It is divided into photoaging. Changes and reductions in collagen, a major structural component of the skin, are known to be a major cause of clinical changes such as reduction of wrinkles and elasticity of the skin and are observed in both natural and photoaging. This collagen deficiency during the natural aging process is known to occur due to a decrease in the production itself or promotion of degradation by an increase in the type I collagenase (MMP-1, Matrix Metalloproteinase-1). Ultraviolet irradiation induces the biosynthesis of type I collagenase in the skin, thereby increasing type I collagenase promotes the breakdown of dermal collagen and further promotes the breakdown of the extracellular matrix (ECM). Therefore, restoring collagen deficiency in older human skin is considered as a method of restoring skin aging. Thus, aging symptoms, ie, wrinkles of the skin, may be promoted by promoting collagen biosynthesis or reducing MMP-1 biosynthesis. It can prevent or restore the formation, elasticity and sagging. Gompi (Ecklonia stolonifera) used in the present invention (Ecklonia stolonifera) is a perennial seaweed belonging to the brown algae of the Laminariaceae, which grows on rocks within 10 meters of water, also called sesame seaweed and has been used for food. It is a special species of the southern coast of Korea, and is a perennial seaweed that grows in the crawling area as well as the reproduction by the rejuvenator like seaweed and kelp. Recently, research has been actively conducted on the development of related medicines because it contains a large amount of fucoidan, which is known to have anticancer and blood purification effects in gompi. In addition, Gompi is used as a feed for complex farms such as abalone farms, lobster farms, and fish cage farms, which mainly use raw feed, and can be used for eco-friendly farming, such as removing pollutants from the seas where eutrophication continues. In general, seaweeds have been widely used as insecticides, gout treatments, anticonvulsants, and gallstones. In studies of gompi, ecstolonol and phloofucofuroeckol A have been published (Arch Pharm Res, 2005, 28: 1376-1380), tyrosinase as a hepatoprotectant. (tyrosinase) inhibitors and ingredients known for antioxidant effects include phloroglucinol, ecstolonol, eckol, phlorofucofuroeckol A, dieckol, and the like. Reported (Arch Pharm Res, 2004, 27: 1226-1232, Arch Pharm Res, 2004, 27: 194-198). In Gompi, phlorotannin and eclonialactone, such as phlorotglucinol, have been reported and isolated, but little other research has been done. Looking at the patent for gompi, cosmetics for whitening using tyrosinase inhibitory activity (Korean Patent No. 0542751, Japanese Laid-Open Patent No. 1992-235110), anti-aging pharmaceutical composition using antioxidant (Korean Patent No. 0512482), chromosomal abnormalities Inhibitor (Korean Patent No. 0152738), Liquid organic fertilizer for preventing pests (JP-A-2004-189532), Gompi production growth method (JP-A-1998-000042), Anti-viral agent (JP-A-2004-189648) No.), an antimicrobial agent (Japanese Patent Laid-Open No. 2003-277203) and the like have been registered or published. However, little research has been done on aging of the skin using gompi extract. Therefore, it is an object of the present invention to identify the use of gompi extract to provide for external application for skin, food or pharmaceutical use. The present inventors showed that the extract of Gompi from seaweeds growing in the sea showed excellent inhibitory effects of NF-kappa (NF-kB) and AP-1 (AP-1), decreased biosynthesis of type I collagenase, and biosynthesis of collagen. Increasing and confirming that there are no side effects, the present invention was completed.

It is an object of the present invention to provide a new use of Gompi extract and Ecole, Diecol as a skin external preparation for skin wrinkle improvement and elasticity improvement.

In order to achieve the above object, the present invention has a function to improve wrinkles, and provides an extract of Gompi, ethanol, and diecol extracted with water, alcohol or a mixture thereof.

In addition, the present invention has a function of improving elasticity, and provides an extract of Gompi, Ecole, and Diecol extracted with water, alcohol or a mixture thereof.

In addition, the present invention has an inflammation inhibitory function, and provides an extract of Gompi, Ecole, and Diecol, which are extracted with water, alcohol or a mixture thereof.

In addition, the present invention provides a cosmetic for improving skin aging comprising an extract of Gompi and Ecole, Diechol as an active ingredient.

In addition, the present invention provides cosmetics for improving wrinkles comprising Gompi extract and Ecole, Diechol as an active ingredient.

In addition, the present invention provides cosmetics for improving elasticity comprising the extract of Gompi and Ecole, Diechol as an active ingredient.

In another aspect, the present invention provides a healthy food for improving skin aging comprising an extract of Gompi and Ecole, Diechol as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides an extract, Ecole, and Diecol, which have a function of improving skin aging.

The present inventors extracted the dried Gompies outpost to investigate the effect of Gompi extract on skin aging improvement. The method of extracting gompi may be used by a general extraction method known in the art, and extracted by the following extraction method: 1) obtaining an extract from gompi using water, alcohol or a mixture thereof; 2) filtering the extract of step 1); 3) concentrating the solution of step 2). The alcohol in step 1) is preferably 100% methanol, preferably 5 to 10 times the volume. The extraction time is preferably 2 to 24 hours, most preferably 3 hours. Gompi extract showed excellent N-Kappaby and Api-1 inhibitory activity (see Table 1 and Figure 1), MMP-1 biosynthesis inhibitory effect (see Tables 2,3 and 2,3,4) and collagen biosynthesis Increasing (see Table 4 and Figure 5) shows degradation inhibition effect (see Table 5 and Figure 6). Through this, Gompi extract can be usefully used as an effective skin external preparation, drink and health food that can improve the skin wrinkles and elasticity by inhibiting the degradation of the extracellular matrix of the skin and promoting biosynthesis.

The present invention provides cosmetics for anti-aging comprising the extract of Gompi having the skin wrinkles and elasticity improvement function.

In the preparation of the anti-aging cosmetics containing the gompi extract of the present invention, the gompi extract of the present invention can be added to 0.01 to 15 parts by weight, preferably 0.1 or 10 parts by weight to the external composition for skin to be contained. In addition to the Gompi extract, the skin external preparation of the present invention includes fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, Commonly used in ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for the skin It may contain adjuvants conventionally used in the field of dermatology, such as any other ingredient. The ingredients may also be introduced in amounts generally used in the field of dermatology.

The present invention provides a healthy food for wrinkle improvement, elasticity improvement comprising the extract of Gompi as an active ingredient to improve skin aging.

As a health food containing the gompi extract which functions to improve the skin aging of the present invention, there are folk remedies for health purposes such as tea, jelly, juice, extract, beverage, etc., wherein the gompi extract is an active ingredient. As described above, the health food of the present invention processed in various forms is not only excellent in skin aging improvement and recovery action without side effects on the human body, and can be easily used for long-term storage and can be useful for wrinkle improvement and elasticity improvement.

Gompi extract having a skin aging improvement function of the present invention can be added to the skin care health food for the purpose of wrinkle improvement and elasticity improvement. When the gompi extract of the present invention is used as a food additive, the gompi extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, in the preparation of food or beverages, the Gompi extract of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and there is no problem in terms of safety, so the active ingredient may be used in an amount above the above range.

There is no particular limitation on the kind of food. Examples of foods to which the skin aging extract can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, Drinks, teas, drinks, alcoholic beverages and vitamin complexes, etc., and includes all healthy foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors, natural carbohydrates, and the like as additional components, as in a conventional beverage. Natural carbohydrates described above are glucose, monosaccharides such as fructose, malsaccharides, disaccharides such as sucrose, and polysaccharides such as dextrin, cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin, aspartame, and the like can be used. The proportion of natural carbohydrates is generally from 0.01 to 0.04 g, preferably from about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

The composition of the present invention other than the above is a variety of nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols And carbonating agents used in carbonated drinks. In addition, the composition of the present invention may contain a pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention. Gompi extract having a skin-improving effect of the present invention can be administered orally or parenterally during clinical administration, and one or two or more pharmaceutically acceptable conventional carriers or one or more in an effective amount of the main extract Two or more additives may be selected to prepare the compositions of conventional pharmaceutical formulations. In the present invention, the carrier and the additive can be used in all pharmaceutically acceptable, and specifically, as a diluent, lactose monohydrate, corn starch, cornbean oil, soybean oil, microcrystalline cellulose Or mannitol (D-mannitorl) is preferable, and magnesium stearate or talc is preferable as a lubricant, and polyvinylpyrrolidone (PVP: polyvinyipyrolidone) or hydroxypropyl cellulose (HPC: hydroxypropylcellulose) is preferable. Is preferred. In addition, the disintegrant may be selected from among carboxymethylcellulose calcium (Ca-CMC), sodium starch glycolate, polyacrylin potassium or cross-linked polyvinylpyrrolidone. Preferably, the sweetener is selected from sucrose, fructose, sorbitol or aspartame, and the stabilizer is carboxymethylcellulose sodium (Na-CMC), beta-cyclodextrin, It is selected from white bee's wax or xanthan gum, and preservatives include methyl p-hydroxy benzoate and methylparaben, propyl p-hydroxy benzoate and propylparaben. It is preferable to select from potassium sorbate. The extract of Gompi, which functions to improve the skin aging of the present invention, inhibits the activities of F-kappa (NF-kB) and AP-1 (AP-1) in vivo, reduces the biosynthesis of MMP-1, It is used for the purpose of efficiently increasing biosynthesis and inhibiting decomposition, and is not particularly limited in the formulation. For example, softening water, nourishing cream, massage cream, nourishing cream, pack, gel or skin adhesive type makeup It may be a cosmetic composition having a formulation of the table, and may be used in a transdermal dosage form such as lotion, ointment, gel, cream, patch or spray, and may be used as a beverage, powder, capsule, soft capsule, tablet, gum, or adhesive type liquid composition. It may be used, and may be used in the preparation of powders, pills, tablets, powders, granules and the like. On the basis of in vitro and in vivo experiments, it is preferable that the total weight of the skin external preparation composition including the Gompi extract as an active ingredient is 0.0001 to 50 parts by weight, and most preferably 0.01 to 5 parts by weight. Gompi extract having a skin aging improvement function of the present invention may further contain one or more active ingredients exhibiting the same or similar functions. In addition to the above main components, the Gompi extract having the function of improving skin aging of the present invention contains minerals such as vitamins B, C, E or beta carotene, Ca, Mg, Zn, phospholipids such as lecithin or maltol, oligosaccharides, Compounds such as amino acids can be used, and among them, the use of 2-3 components in vitamin C, E, beta carotene, maltol and the like is more preferable because it can enhance the biological activity effect. In addition to the above ingredients, in order to enhance the taste as a known additive, natural flavors such as plum, lemon, pineapple or herb, or natural fruit juice, natural pigments such as chlorophyllin and flavonoids, and fructose, sweet sugar Can be mixed with honey, sugar alcohol, sugar and acidulants such as citric acid and sodium citrate. Hereinafter, the present invention will be described in detail by examples and production examples. However, the following Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples.

< Example  1> Gompi Extract Manufacturer

1 Kg of dried Gompie outpost was made into powder, placed in an extraction bag, immersed in 10% of 100% methanol, extracted under reflux three times for 3 hours, cooled to room temperature, and filtered through a filter paper. The extract was concentrated using a concentrator under reduced pressure until the solution completely evaporated, thereby obtaining crystals. As a result of using the above method, 103 g of extract from 1 Kg of dried Gompie outpost could be prepared.

< Example  2-3> Ecole, Diecole  Produce

1 Kg of dried Gompie outpost was made into powder, placed in an extraction bag, immersed in 10% of 100% methanol, extracted under reflux three times for 3 hours, cooled to room temperature, and filtered through a filter paper. The extract was concentrated using a concentrator under reduced pressure until the solution completely evaporated, thereby obtaining crystals. The extract is suspended in 1 liter of water, and then added with 1 liter of ethyl acetate (EA) and shaken again. The extracts dissolved in ethyl acetate were dried under reduced pressure, and silica gel chromatography was used to obtain subfractions. The subfractions showing inhibitory activity of type I collagenase were identified. The active substance was found by hanging the ODS open column and confirmed by eckol (example 2) and diecol (example 3). As a result of using the method, 53 mg of Example 2 and 46 mg of Example 3 were prepared in 1 Kg of dried Gompie outpost.

< Experimental Example  1> Nf - Kappabi and Api Inhibitory effect on -1

In order to determine the inhibitory effect on the anti-inflammatory or antioxidant transcription factors NF-kBabi (NF-kB) and AP-1 (AP-1) of the Gompi extract of Example 1 prepared by the above method A reporter gene assay was performed using plasmids bound to luciferase. Briefly, cervical cancer cell lines (HeLa cells) are seeded in 24-well plates and incubated for 24 hours in DMEM medium containing 10% fetal calf serum and 1% penicillin / streptomycin. After the transcription factor is activated, the plasmid NF-kB-luciferase or AP-1-luciferase reporter plasmid DNA (Stratagene), which is designed to report it as luciferase, is transfected with Lipofectamin transfection reagent (Invitrogen). After 24 hours of transfection, Example 1 was added to the medium at the concentrations in Table 1 below, and stimulated with PMA (Phobol ester). After 24 hours, the cells were lysed and luciferase activity was measured by a luminometer.

Gompi Transcription Factors of Extracts Inhibitory ability density NF-kB AP-1 Example 1 10ug / ml 76% 67% 1ug / ml 52% 49%

From Table 1, Gompi extract is determined to have an antioxidant and anti-inflammatory effect by efficiently inhibiting NF-kB and AP-1. This will be useful for anti-aging effects such as inflammation suppression, wrinkles and elasticity improvement.

< Experimental Example  2> Type I Collagenase (MMP-1)  Measurement of biosynthesis inhibitory efficacy ( in in vitro )

Human fibroblasts were cultured in 6-well plates at a concentration of 10 ⁵ , and after 24 hours, the tumor necrosis factor (TNF α, Tumor necrosis factor α) was 20ng / ml, and the extract according to Example 1-3 was It was replaced with the medium containing. On day 1 of culture, cells were collected and subjected to reverse transcription PCR (RT-PCR) to measure and quantify RNA for transcribed MMP-1. The results are shown in Table 2, a comparison value measured with a densitometer with a TNF α control group of 100.

Bear Blood Extract , Cole, Diecole  Type I Collagenase  Effect on Biosynthesis Concentration ( ug Of ml ) MMP-1 biosynthesis (%) Example 1 Example 2 Example 3 10 35 17 8 One 43 38 27 0.1 70 56 39 Control 100 100 100

From the Table 2, gompie starch extract or Ecole, diecol component concentration-dependently reduce the biosynthesis of type 1 collagenase caused by tumor necrosis factor, effectively preventing or improving skin matrix material reduction, elasticity reduction, wrinkle formation, etc. It can be seen that.

< Experimental Example  3> Type I by UV Collagenase (MMP-1)  Measurement of biosynthesis inhibitory efficacy ( in in vitro )

Human fibroblasts were cultured in 48 well plates at a concentration of 10 ⁴ , and after 24 hours, irradiated with UV A at 15 J / cm ² , the extract according to Example 1-3 was replaced with a medium containing the following concentrations. The supernatant was harvested on day 2 of culture and the amount of type I collagenase produced was quantified using the ELISA (AP biotech RPN2610) method. The results are shown in Table 3 comparing the UV control group to 100.

Bear Blood Extract , Cole, Diecole  Type I by ultraviolet Collagenase  Effect on Biosynthesis Concentration ( ug Of ml ) MMP-1 biosynthesis (%) Example 1 Example 2 Example 3 10 30 15 6 One 40 34 25 0.1 68 52 35 Control 100 100 100

Gompie starch extract or Ecole, diecol component from Table 3 reduces the biosynthesis of type 1 collagenase due to UV-A concentration-dependently, effectively prevents the reduction of skin matrix material, decrease elasticity, wrinkles, etc. caused by UV Or it can be seen that it can be improved.

< Experimental Example  4> In skin cells  Collagen Biosynthesis Efficacy

Human fibroblasts were cultured in 48 well plates at a concentration of 10 ⁴ , and after 24 hours, the extract according to Example 1-3 was replaced with a medium containing the following concentrations. The supernatant was harvested on day 2 of culture and the amount of procollagen produced was quantified using the ELISA (Takara MK101) method. The results are shown in Table 4, the comparison of the control was not treated as 100.

Bear Blood Extract , Cole, Diecole  Collagen production promoting effect Concentration ( ug Of ml ) Procollagen Biosynthesis (%) Example 1 Example 2 Example 3 10 186 192 205 One 164 170 182 0.1 139 145 152 Control 100 100 100

From Table 4, it can be seen that gompi extract, ethanol and dietol are effective in improving wrinkles and elasticity of skin by promoting collagen biosynthesis in a concentration-dependent manner.

< Experimental Example  5> Determination of Collagen Inhibition Effect

Human fibroblasts were cultured in 12 well plates at 10 ⁵ concentrations, and after 24 hours, the tumor necrosis factor (TNF α, Tumor necrosis factor α) was 20ng / ml, and the extract according to Example 1-3 was It was replaced with the medium containing. The supernatant was harvested on day 2 of culture and the amount of procollagen remaining without degradation was measured using Western blot method. The results are shown in Table 5, a comparison value measured with a densitometer with a TNF α control group of 100.

Bear Blood Extract , Cole, Diecole  Collagen Degradation Inhibitory Effect Concentration ( ug Of ml ) Procollagen Biosynthesis (%) Example 1 Example 2 Example 3 10 210 236 257 One 178 198 212 0.1 152 169 179 Control 100 100 100 No treatment 200 200 200

From Table 5, it can be seen that Gompi extract, Ecole, and Diecol can effectively control the decomposition of collagen by TNF α and control it in a concentration-dependent manner. Based on the results of the test examples described above, by containing the Gompi extract, ethanol, and dietol, the composition of the cosmetic and pharmaceutical formulations, food, beverages of various formulations to improve skin wrinkles and elasticity. However, the composition of the present invention is not limited only to the following examples.

< Formulation example  1> Preparation of external preparation for skin

1. Flexible Cosmetic (Skin Lotion)

Purified Water To 100 (wt%)

Bear blood extract / Ecole / Diecol 1 / 0.1 / 0.1 parts by weight

Butylene glycol 2.0 parts by weight

Propylene glycol 2.0 parts by weight

Carboxy vinyl polymer 0.1 parts by weight

0.2 parts by weight of Fiji-12 nonylphenyl ether

Polysorbate 80 0.4 parts by weight

10.0 parts by weight of ethanol

0.1 parts by weight of triethanolamine

Preservatives, colorings, flavors

2. Softening Cosmetics (Milk Lotion)

Purified Water To 100 (wt%)

Bear blood extract / Ecole / Diecol 1 / 0.1 / 0.1 parts by weight

Beeswax 4.0 parts by weight

Polysorbate 60 1.5 parts by weight

1.5 parts by weight of sorbitan sesquioleate

0.5 part by weight of liquid paraffin

Caprylic / Capric Triglyceride 5.0 parts by weight

Glycerin 3.0 parts by weight

Butylene glycol 3.0 parts by weight

Propyl glycol 3.0 parts by weight

Carboxy vinyl polymer 0.1 parts by weight

0.2 parts by weight of triethanolamine

Preservatives, colorings, flavors

3. Nutrition Cream

Purified Water To 100 (wt%)

Bear blood extract / Ecole / Diecol 1 / 0.1 / 0.1 parts by weight

10.0 parts by weight of beeswax

Polysorbate 60 1.5 parts by weight

Sebum 60 Cured Castor Oil 2.0 parts by weight

0.5 parts by weight of sorbitan sesquioleate

10.0 parts by weight of liquid paraffin

Squalane 5.0 parts by weight

Caprylic / Capric Triglyceride 5.0 parts by weight

Glycerin 5.0 parts by weight

Butylene glycol 3.0 parts by weight

Propylene glycol 3.0 parts by weight

0.2 parts by weight of triethanolamine

Preservatives, colorings, flavors

4. Massage Cream

Purified Water To 100 (wt%)

Bear blood extract / Ecole / Diecol 1 / 0.1 / 0.1 parts by weight

10.0 parts by weight of beeswax

Polysorbate 60 1.5 parts by weight

Sebum 60 Cured Castor Oil 2.0 parts by weight

0.8 parts by weight of sorbitan sesquioleate

Squalane 40.0 parts by weight

Caprylic / Capric Triglyceride 4.0 parts by weight

Glycerin 5.0 parts by weight

Butylene glycol 3.0 parts by weight

Propylene glycol 3.0 parts by weight

0.2 parts by weight of triethanolamine

Preservatives, colorings, flavors

5. Pack

Purified Water To 100 (wt%)

Bear blood extract / Ecole / Diecol 1 / 0.1 / 0.1 parts by weight

13.0 parts by weight of polyvinyl alcohol

0.2 parts by weight of sodium carboxymethyl cellulose

Glycerin 5.0 parts by weight

Allantoin 0.1 part by weight

6.0 parts by weight of ethanol

0.3 parts by weight of Fiji-12 nonylphenyl ether

Polysorbate 60 0.3 parts by weight

Preservatives, colorings, flavors

< Formulation example  2> Preparation of Pharmaceutical Formulations

 1. Preparation of powder

Bear blood extract / Ecole / Diecol 2 g

1 g lactose

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

2. Preparation of Tablets

Gompi extract / Ecole / Diecol 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional tablet production method.

3. Preparation of Capsule

Gompi extract / Ecole / Diecol 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

< Formulation example  3> Manufacture of food

Food products containing the extract of the present invention / Ecole / dietol was prepared as follows.

1. Preparation of Cooking Seasonings

Bear blood extract / ethanol / dietol of the present invention to prepare a seasoning for improving skin aging to 0.1-50 parts by weight.

2. Manufacturing of Flour Foods

0.1 to 5 parts by weight of the Gompi extract / ethanol / dietol of the present invention was added to the flour, using this mixture to prepare bread, cakes, cookies, crackers and noodles to prepare a health-promoting food.

3. Preparation of soups and gravy

0.1 to 5 parts by weight of gompi extract / ethanol / dietol of the present invention was added to soups and broth to prepare health products, health soup and soup.

4. Manufacture of dairy products

0.1 to 10 parts by weight of the Gompi extract / Ecole / Diecol of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

5. Manufacture of wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then powdered into powder having a particle size of 60 mesh.

The Gompi extract / Ecole / Diecol of the present invention was depressurized and concentrated in a vacuum concentrator, and the dried product obtained by spraying and drying with a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

Cereals, seeds and Gompi extract / Ecole / Diecol prepared above were combined and prepared in the following ratios.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight perilla, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

Dry powder (3 parts by weight) of Gompi extract / Ecole / diecol

Ganoderma lucidum (0.5 parts by weight),

Sulfur (0.5 part by weight).

< Formulation example  4> Manufacture of beverage

1. Preparation of carbonated drinks

5 to 10 parts by weight of sugar, 0.05 to 0.3 parts by weight of citric acid, 0.005 to 0.02 parts by weight of caramel, 0.1 to 1 parts by weight of vitamin C, and 0.1 to 5 parts by weight of the extract from Gompi extract / Ecole / diecol and mixed with the remaining parts of Purified water was mixed to make a syrup, and the syrup was sterilized at 85 to 98 ° C. for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 4, and then 0.5 to 0.82 parts by weight of carbon dioxide was injected into the extract of the present invention. Carbonated beverages containing diecol were prepared.

2. Manufacture of health drinks

0.1-10 parts by weight of Gompi extract / Ecole / diecol, 0.5 parts by weight of liquid fructose, 2 parts by weight of oligosaccharides, 2 parts by weight of sugar, 0.5 parts by weight of salt, and the remaining weight parts of water are mixed homogeneously, followed by instant sterilization. Healthy drinks were prepared by packaging in small packaging containers such as glass bottles and PET bottles.

3. Preparation of Vegetable Juice

0.1-5 g of Gompi extract / Ecole / Diecol of the present invention was added to 1 L of tomato or carrot juice to prepare a vegetable juice for health promotion.

4. Preparation of Fruit Juice

0.1-1 g of Gompi extract / Ecole / Diecol of the present invention was added to 1 L of apple or grape juice to prepare a fruit juice for health promotion.

As described above, gompi extract, ecol and diecol, which are extracted with water, alcohol or a mixture thereof, inhibit the transcription factors n-kappabi (NF-kB) and api-1 (AP-1), It inhibits type I collagenase (MMP-1), a degrading enzyme of the skin matrix, and increases collagen, which is a major constituent of the skin, which has the effect of inhibiting skin inflammation, improving wrinkles, and improving elasticity. It can be used, food and pharmaceutical can also be usefully used without side effects.

Claims (9)

Gompi extract, which has a function of improving skin aging and is extracted with water, alcohol or a mixture thereof. Gompi extract with anti-inflammatory activity, extracted with water, alcohol or mixtures thereof. Ecole with skin aging improvement function. DieCole has a skin-aging effect. The gompi extract according to claim 1 or 2, wherein the alcohol is a C ₁ to C ₃ lower alcohol. The external preparation for improving wrinkles comprising at least one of the Gompi extract, Ecole, Diechol as an active ingredient. An external preparation for improving elasticity comprising at least one of the extract, Ecole, Diechol as an active ingredient. Health food for improving skin aging comprising at least one of the extract, Ecole, Diechol as an active ingredient. The method of claim 1 to 4, wherein the formulation containing at least one of gompi extract, ethanol, dietol, characterized in that it is formulated in a formulation selected from the group consisting of tablets, pills, powders, granules, capsules and liquids.

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