KR102715184B1 - Functional compositions comprising diascorea bulbifera extract and plant-derived exosomes - Google Patents

Abstract

The present disclosure relates to a functional composition comprising an extract of the sky berry and plant-derived exosomes, and more specifically, to a functional composition which has an excellent moisturizing effect and can be used as a cosmetic raw material and a health functional food raw material having an excellent cell regeneration effect by comprising an extract of the sky berry and plant-derived exosomes.

Description

{FUNCTIONAL COMPOSITIONS COMPRISING DIASCOREA BULBIFERA EXTRACT AND PLANT-DERIVED EXOSOMES}

The present disclosure relates to a functional composition comprising an extract of the sky berry and plant-derived exosomes, and more specifically, to a functional composition which has an excellent moisturizing effect and can be used as a cosmetic raw material and a health functional food raw material having an excellent cell regeneration effect by comprising an extract of the sky berry and plant-derived exosomes.

Dryness of the skin due to environmental and genetic factors can weaken the functionality of the skin barrier and cause diseases including various inflammations, so it is important to moisturize the skin so that it can maintain an appropriate level of moisture. In particular, since it is not easy to maintain an appropriate level of moisture in the skin due to various weather environments, living environments, and various biological factors, various cosmetics for moisturizing are provided.

In order to provide moisturizing effects to such cosmetics, various chemically manufactured moisturizers and animal-derived moisturizers have been used. However, chemically manufactured moisturizers have the problem of lacking eco-friendliness in the manufacturing process, and there have been cases of human toxicity and environmental toxicity to workers due to the use of various chemical raw materials, and even when they are added to cosmetics to provide moisturizing effects, there is ongoing controversy over the safety of chemical substances on the skin and human body when used, and in particular, various damage cases to sensitive skin continue to be reported, and as a result, they are being ignored by consumers. In addition, animal-derived moisturizers do not fit the modern trend of great interest in animal protection, and as a result, they are also being ignored by consumers.

Meanwhile, Diascorea bulbifera , also called fruit yam and creeping yam, is a plant that contains an abundance of useful substances such as mucin, saponin, choline, arginine, dietary fiber, potassium, magnesium, calcium, minerals, and diosgenin. Despite the excellent functions and potential of Diascorea, its functionality has not been properly studied to date, and research on various functional products utilizing it is also lacking.

In addition, exosomes can be classified into animal-derived exosomes and plant-derived exosomes, among which plant-derived exosomes are nano-sized particles secreted from plant cells and play a certain role in intercellular movement and absorption, and contain unique effective ingredients of the plant, so that indirect adoption of the effective ingredients is possible depending on which plant the exosome is derived from, and through this, the useful functions of the plant can be enjoyed. Furthermore, plant-derived exosomes have the advantages of easy mass production and high stability compared to animal-derived exosomes.

Under these circumstances, there is an urgent need for a functional composition containing sky moss and plant-derived exosomes as a cosmetic raw material that has excellent skin moisturizing effects and has beneficial ingredients for the skin, such as anti-inflammatory effects and skin repair effects, without using chemically manufactured moisturizers and animal-derived moisturizers.

The problem that the present disclosure seeks to solve is to provide a functional composition comprising a sky-ma extract and plant-derived exosomes having excellent moisturizing and cell regeneration effects that can be used as cosmetic raw materials.

Another problem that the present disclosure seeks to solve is to provide a functional composition comprising a sky-ma extract and plant-derived exosomes having low skin irritation and excellent skin penetration enhancing effect.

Another problem that the present disclosure seeks to solve is to provide a functional composition that can be used as a health functional food raw material including a sky horse extract and plant-derived exosomes.

The problems to be solved by the present disclosure are not limited to the problems mentioned above, and problems to be solved by the present disclosure that are not mentioned will be clearly understood by a person having ordinary knowledge in the technical field to which the present disclosure belongs (“ordinary skilled in the art”) from the description below.

According to one embodiment of the present disclosure, a functional composition for a cosmetic raw material including a lotus extract and plant-derived exosomes can be provided, characterized in that the plant-derived exosomes are exosomes derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

For example, a functional composition for cosmetic raw materials can be provided, characterized in that the sky horse extract is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

For example, the extract of the herb may be obtained by extracting dried or raw herb using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

For example, the above-mentioned sky horse extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky-ma extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky-ma extract.

For example, the above-mentioned sky horse extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky horse extract may contain arginine in an amount of 0.01 to 0.50 parts by weight based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky-ma extract may contain diosgenin in an amount of 0.001 to 0.003 parts by weight based on 100 parts by weight of the entire sky-ma extract.

For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

According to another embodiment of the present disclosure, a health functional food composition comprising a sky horse extract and a plant-derived exosome, wherein the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica can be provided.

For example, a composition for a health functional food can be provided, characterized in that the extract of the sky horsetail is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

For example, the extract of the herb may be obtained by extracting dried or raw herb using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

For example, the above-mentioned sky horse extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky-ma extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky-ma extract.

For example, the above-mentioned sky horse extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky horse extract may contain arginine in an amount of 0.01 to 0.50 parts by weight based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky-ma extract may contain diosgenin in an amount of 0.001 to 0.003 parts by weight based on 100 parts by weight of the entire sky-ma extract.

For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

According to another embodiment of the present disclosure, the sky horse extract may be a sky horse extract fermented solution obtained by inoculating the sky horse extract with Bacillus subtilis.

According to another embodiment of the present disclosure, a diet food including a sky horse extract or a fermented product using a sky horse extract can be provided.

According to another embodiment of the present disclosure, a composition for health functional food comprising a sky horse extract or a fermented product using a sky horse extract can be provided.

According to the present disclosure, a functional composition including a sky-ma extract and plant-derived exosomes having excellent moisturizing effects and cell regeneration effects that can be used as cosmetic raw materials can be provided.

In addition, according to the present disclosure, a functional composition including a sky-ma extract and plant-derived exosomes having low skin irritation and excellent skin penetration enhancing effect can be provided.

In addition, according to the present disclosure, a functional composition that can be used as a health functional food raw material including a sky horse extract and plant-derived exosomes can be provided.

The excellent and/or useful effects according to the present disclosure are not limited to the effects of the present disclosure described above, and those skilled in the art will also be able to obviously recognize other excellent and/or useful effects of the present disclosure that are not explicitly disclosed in the present specification based on the disclosure of the present specification, and it should be understood that these are intentionally disclosed by the present specification and are obviously included in the scope of the present disclosure.

Figure 1 is a diagram exemplarily showing the composition of a functional composition according to the present disclosure.

The terms or words used in this specification and claims are not intended to be construed as being limited to their commonly used dictionary meanings, and it will be apparent to those skilled in the art that such terms or words are used in a sense intended to convey the meaning of the present disclosure within the scope of the concepts that this specification and claims are intended to clearly convey.

In addition, it will be clearly understood by those skilled in the art that the configurations described in the aspects, embodiments, examples, etc. of the present disclosure described in this specification are merely preferred examples presented at the time to enable those skilled in the art to understand and reproduce the present disclosure, and are not intended to limit the present disclosure thereto.

In addition, the description and specific embodiments of each configuration described in this specification can be obviously applied to the description and specific embodiments of each other configuration. That is, all possible combinations of the various configurations and specific embodiments disclosed in this specification will be clearly understood by those skilled in the art to fall within the scope disclosed in this specification.

The term "and/or" as used herein is a term that includes each and every combination of two or more of the items mentioned. In addition, when a singular term is used herein, it is disclosed including the plural unless otherwise stated.

The terms "comprise" and "comprising" as used herein are terms which allow the presence or addition of other items other than the items mentioned, and the terms "consist," "consist," "consisting," and "consisting" as used herein are terms which do not allow the presence or addition of other items other than the items mentioned.

The term "to" as used herein indicates a numerical range in which the numerical range indicated by using the term "to" includes the values described before and after the term as lower limits and upper limits, respectively. When the upper limit and/or lower limit of any numerical range are each disclosed as multiple values, the numerical range is disclosed as having any one of the multiple lower limit values and any one of the multiple upper limit values as the lower limit and the upper limit, respectively.

The terms “about” and/or “approximately” as used herein mean a numerical range between an upper value and a lower value that is 10% greater than the value indicated using “about” and/or “approximately” above.

The terms or words used in this specification and the claims should not be interpreted as limited to their usual dictionary meanings, but should be interpreted as meanings and concepts that conform to the technical idea of the present disclosure based on the principle that the inventor can appropriately define the concept of the term in order to explain his or her own invention in the best way. Therefore, it should be understood that the configurations described in the embodiments described in this specification are only preferred embodiments of the present disclosure and do not represent all of the technical idea of the present disclosure, and that there may be various equivalents and modified examples that can replace them at the time of this application.

Meanwhile, each description and embodiment disclosed in this specification can also be applied to each other description and embodiment. That is, all combinations of various elements disclosed in this specification fall within the scope of this disclosure, and descriptions omitted in one embodiment can be interpreted in the same manner as described in other embodiments. In addition, the scope of this disclosure cannot be considered limited by the specific description described below.

According to one embodiment of the present disclosure, a functional composition for cosmetic raw materials including a sky horse extract and plant-derived exosomes can be provided.

For example, the functional composition for a cosmetic raw material can be provided as a functional composition including a sky-ma extract and a plant-derived exosome, wherein the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

Sky yams are a type of yam that are thicker and longer than regular yams, and have the characteristics of growing in a round shape without peeling off the skin. They grow mainly in warm regions, especially in tropical and subtropical regions, and since they are yam that bears fruit rather than in the ground, they are easy to cultivate, and they have the characteristics of being able to be grown organically without pesticides. In addition, sky yams have various nutrients and health benefits, and contain a large amount of various effective ingredients. In particular, sky yams as a cosmetic ingredient contain diosgenin, which can slow down the aging process of the skin, keep the skin moisturized, and contain mucin, a sticky mucus component, which can make the skin smooth and prevent various skin diseases.

Plant-derived exosomes are small extracellular vesicles secreted by cells to transmit information between cells. They are responsible for transporting various biological substances such as proteins, lipids, and nucleic acids. They also have the capabilities of stem cells, such as cell differentiation, regeneration, and immunity. When used as a cosmetic ingredient, they can be useful for improving skin health and treating inflammatory diseases.

For example, a functional composition for cosmetic raw materials can be provided, characterized in that the sky horse extract is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

If the amount of the above-mentioned sky-ma extract is less than 5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of the sky-ma extract is minimal, and if the amount of the above-mentioned sky-ma extract is more than 98 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the viscosity of the composition increases and the processability decreases. Therefore, it is preferable that the content of the above-mentioned sky-ma extract satisfies the above-mentioned numerical range.

If the plant-derived exosomes are included in an amount of less than 0.01 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of the plant-derived exosomes is minimal, and if the plant-derived exosomes are included in an amount of more than 2.0 parts by weight based on 100 parts by weight of the entire functional composition, deterioration of the entire composition may occur due to denaturation of the included plant-derived component. Therefore, it is preferable that the content of the plant-derived exosomes satisfies the above numerical range.

For example, the above-mentioned sky ginseng extract may be obtained by extracting dried sky ginseng or raw sky ginseng using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

For example, the above-mentioned sky yam extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky yam extract. The above-mentioned mucin is a sticky component present in sky yam and is a type of glycoprotein, which has an excellent skin moisturizing effect, helps digestive health, can improve symptoms of gastric ulcer or hyperacidity, and can be used as a tonic that increases stamina, as well as helps digestive health, protects the gastric mucosa, and can promote digestion by activating digestive enzymes.

For example, the above-mentioned sky-ma extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky-ma extract. The above-mentioned saponin is an amphipathic glycoside present in various plant species and is a representative compound that enhances the immunity of the human body, and is also used as a cardiotonic or diuretic in oriental medicine.

For example, the above-mentioned sky hawk extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky hawk extract. The above-mentioned choline is a member of the vitamin B complex, a complex lipid component such as lecithin or plasmalogen, and a component of acetylcholine, a substance involved in nerve excitation transmission, and is used for various fat metabolism diseases as a fatty liver prevention factor, and is known to have a positive effect on brain activity and development.

For example, the above-mentioned sky hawk extract may contain 0.01 to 0.50 parts by weight of arginine based on 100 parts by weight of the entire sky hawk extract. The above-mentioned arginine is an alpha-amino acid used in the biosynthesis of proteins and is also called arginine. It is known to strengthen mental and physical functions and promote hormone secretion, and is known to have the effect of nourishing and tonic and to be useful for improving stamina.

For example, the above-mentioned sky-ma extract may contain 0.001 to 0.003 parts by weight of diosgenin based on 100 parts by weight of the entire sky-ma extract. The above-mentioned diosgenin alleviates menopausal symptoms, and generally, during menopause, as hormones decrease, previously unseen symptoms appear, and diosgenin is used as a synthetic raw material for progesterone, male hormones, cortisone, etc., and provides a positive effect on hormone replenishment, thereby activating the body and alleviating menopausal symptoms.

In addition, the dietary fiber contained in the sky horse improves digestive function and helps control blood sugar levels, and it is rich in various minerals such as calcium and magnesium, which are necessary for bone health and heart health, and it also contains many essential amino acids, which can help muscle and bone health.

In addition, the saponin and dietary fiber contained in the sky hawkweed reduce the amount of LDL cholesterol, which is negative cholesterol for the human body, and increase the amount of HDL cholesterol, which is positive cholesterol, in the body. They also prevent waste from accumulating in the blood vessels, purifying the blood, and potassium helps to manage blood pressure stably by excreting sodium from the body, which can prevent cardiovascular disease.

For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

Carrot seed oil is an oily component extracted from carrot seeds, and although it has a strong, characteristic odor, it is a component that has excellent antioxidant effects and skin moisture control effects, and has recently been confirmed to exhibit various anticancer properties. The composition according to the present disclosure can exhibit excellent skin moisturizing effects and anti-inflammatory effects while suppressing the off-odor of carrot seed oil by including a Sky-ma extract and plant-derived exosomes together with carrot seed oil.

When the carrot seed oil is included in an amount of less than 0.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of carrot seed oil is minimal, and when it is included in an amount of more than 1.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the off-flavor becomes stronger and the degree of skin irritation increases. Therefore, it is preferable that the content of the carrot seed oil satisfies the above numerical range.

For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

The extract of the deciduous broadleaf shrub, the deciduous tree, is a component extracted from the deciduous tree, which has fruits and flowers that have antipyretic and anti-inflammatory effects. It has an antibacterial function and is a component that can soothe mild inflammation of the skin and reduce redness. The composition according to the present disclosure can further enhance the anti-inflammatory effect of the composition by including the extract of the sky horse and the plant-derived exosome together with the deciduous tree extract.

If the above-mentioned extract of the zelkova tree is included in an amount of less than 0.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of the extract of the zelkova tree is minimal, and if it is included in an amount of more than 1.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that it is sticky and leaves a residue. Therefore, it is preferable that the content of the extract of the zelkova tree satisfies the above numerical range.

For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

Senna leaf, also known as senna leaf, is a raw material known to be useful for promoting digestion and relieving constipation, and is also an ingredient that performs various anti-inflammatory and fever-reducing functions. The composition according to the present disclosure can exhibit excellent skin moisturizing effects and anti-inflammatory effects by including a senna leaf extract together with a celery extract and plant-derived exosomes.

There is a problem that the skin improvement effect due to the inclusion of the senna leaf extract is minimal when the senna leaf extract is included in an amount less than 0.02 parts by weight based on 100 parts by weight of the entire functional composition, and there is a problem that skin irritation increases when the senna leaf extract is included in an amount greater than 0.04 parts by weight based on 100 parts by weight of the entire functional composition. Therefore, it is preferable that the content of the senna tree extract satisfies the above numerical range.

According to another embodiment of the present disclosure, a health functional food composition comprising a sky horse extract and a plant-derived exosome can be provided, characterized in that the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

For example, a composition for health functional food can be provided, characterized in that the extract of the sky horsetail is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

For example, the above-mentioned sky ginseng extract may be obtained by extracting dried sky ginseng or raw sky ginseng using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

For example, the above-mentioned sky horse extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky-ma extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky-ma extract.

For example, the above-mentioned sky-ma extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky-ma extract.

For example, the above-mentioned sky horse extract may contain 0.01 to 0.50 parts by weight of arginine based on 100 parts by weight of the entire sky horse extract.

For example, the above-mentioned sky-ma extract may contain diosgenin in an amount of 0.001 to 0.003 parts by weight based on 100 parts by weight of the entire sky-ma extract.

For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

According to another embodiment of the present disclosure, the sky horse extract may be a sky horse extract fermented solution obtained by inoculating the sky horse extract with Bacillus subtilis.

Hereinafter, the present disclosure will be described in more detail using examples. Process conditions and preparation steps not specified in the examples below may be process conditions or preparation steps that are obvious in the technical field to which the present disclosure belongs, and a person skilled in the art will be able to select them without much difficulty based on the present disclosure and reproduce the problem-solving principle of the present disclosure.

에서 진행되며, 각 단계는 통상의 기술자가 별다른 어려움 없이 도출할 수 있는 수단 및 도구에 의해 수행되는 것으로 이해되어야 할 것이다.In addition, in the manufacturing method according to the present disclosure, unless otherwise specified, each step constituting the manufacturing method is performed at room temperature (25

It should be understood that each step is performed by means and tools that can be derived without much difficulty by a person skilled in the art.

Manufacturing Preparation Example 1: Preparation of Raw Sky Horse Extract

Sky yam was grown and used directly. 300g of raw sky yam was cut, 300g of each solvent listed in Table 1 was added, and stirred at room temperature for 8 hours in an extractor equipped with a cooling condenser. This was extracted at 45℃ for 12 hours, and then filtered with a Whatman No. 6 filter paper to obtain a filtrate. The filtrate was concentrated under reduced pressure at 60℃ in a distillation device equipped with a cooling condenser to obtain an extract.

The extraction yield of Skyma according to each solvent is shown in Table 1 below.

menstruum Sky horse extract (g) Example 1 water 320 Example 2 Ethyl acetate 170 Example 3 Butyl acetate 250 Example 4 Ethyl alcohol 330 Example 5 Isopropyl alcohol 230 Example 6 Butyl alcohol 170 Example 7 Ethylene glycol 210 Example 8 Propylene glycol 260 Example 9 Propanol 310 Example 10 Acetone 250 Example 11 benzene 310

Manufacturing Preparation Example 2: Preparation of dried sky horsetail extract

Sky yam was grown and used directly. Using a freeze dryer, dried or naturally dried sky yam 500g was used, and 500g of each solvent in Table 3 was added, and stirred at room temperature for 8 hours in an extractor equipped with a cooling condenser. This was extracted at 45℃ for 12 hours, and then filtered with a Whatman No. 6 filter paper to obtain a filtrate. The filtrate was concentrated under reduced pressure at 60℃ in a distillation device equipped with a cooling condenser to obtain an extract.

The extraction yield of Skyma according to each solvent is shown in Table 2 below.

menstruum Sky horse extract (g) Example 12 water 620 Example 13 Ethyl acetate 370 Example 14 Butyl acetate 350 Example 15 Ethyl alcohol 330 Example 16 Isopropyl alcohol 330 Example 17 Butyl alcohol 370 Example 18 Ethylene glycol 310 Example 19 Propylene glycol 360 Example 20 Propanol 310 Example 21 Acetone 350 Example 22 benzene 310

Manufacturing Example 1: Manufacturing of compositions of Examples 23 to 28 with added plant-derived exosomes

The sky-ma extract obtained from the above Example 1 was left at room temperature, the solid content was removed by sieving, and stabilized. Then, 1.5 parts by weight of the plant-derived exosomes shown in Table 3 below were added and stirred to prepare compositions of Examples 23 to 28.

Additional plant-derived exosome types Example 23 Exosomes from lotus leaves Example 24 Exosomes from lotus root Example 25 Peony-derived exosomes Example 26 Okra-derived exosomes Example 27 Exosomes from goldfish Example 28 Exosomes from the plant

Manufacturing Example 3: Manufacturing of compositions of Examples 29 to 33 with additional ingredients added

The composition of Example 23 was left at room temperature to stabilize, and then the additional components shown in Table 4 below were added in the amounts described, stirred, and mixed to prepare the compositions of Examples 29 to 33.

Unit: parts by weight Carrot seed oil Extract of the yam tree Senna leaf extract Example 29 0.7 - - Example 30 0.7 0.7 - Example 31 0.7 0.7 0.03 Example 32 0.3 - - Example 33 1.2 - -

Manufacturing Example 4: Manufacturing of a fermented extract of Sky Horse

100 kg of raw sky yam was washed with clean water, 500 kg of purified water was added thereto, and a high-speed stirring rotary crusher was used to grind it for 5 minutes. It was filtered using a high-pressure press to obtain 56 kg of the filtrate. 20 kg of the obtained extract was placed in a fermentation tank, and Bacillus subtillis was inoculated while maintaining the temperature at 30 to 37℃ and stirring at 100 to 200 rpm. The fermented sky yam extract was fermented for 30 days to prepare a composition of Example 34.

Experimental Example 1: Skin irritation test (patch test)

The skin irritation of the above-mentioned Sky-ma extract was confirmed by a patch test. The extract extracted with the solvent according to Examples 1 to 11 was applied to a chamber (stiff aluminium) with a diameter of 8 mm and a depth of 0.5 mm, and 5 chambers were attached in 2 rows to 1 row of rectangular tape. The tape was attached to the backs of 30 adults, and the patch was removed after 2 days, and the skin irritation was determined according to the ICDRG criteria.

ICDRG criteria - ?: questionable reaction, +: weakly positive, ++: strongly positive, +++: very strongly positive, -: negative, IR: irritant

Example Skin irritation Example Skin irritation Example Skin irritation Example 1 - Example 12 - Example 23 - Example 2 - Example 13 - Example 24 - Example 3 - Example 14 - Example 25 - Example 4 - Example 15 - Example 26 - Example 5 - Example 16 - Example 27 - Example 6 - Example 17 - Example 28 - Example 7 - Example 18 - Example 29 - Example 8 - Example 19 - Example 30 - Example 9 - Example 20 - Example 31 - Example 10 - Example 21 - Example 32 - Example 11 - Example 22 - Example 33 +

Referring to Table 5 above, it was confirmed that all of the compositions of Examples 1 to 31 according to the present disclosure did not cause skin irritation, and the composition of Example 33 containing carrot seed oil in an amount exceeding the preferred numerical range according to the present disclosure was confirmed to cause skin irritation.

Experimental Example 2: Verification of skin penetration enhancement effect

The skin penetration enhancement effect of the extract of Sky Horse was measured using a transdermal absorption device (Franz type diffusion cell, Hason) targeting guinea pig skin. Compositions 1 to 8 were prepared with the compositions shown in Table 6 below and used as the experimental group, and compositions 9 to 10 were used as the control group.

Unit: parts by weight Pharmacologically active agent menstruum Skin penetration enhancer total Experimental group Composition 1 Raw Sky Extract 1.0 Ethanol 97.00 Cetyl alcohol 2.0 100 Composition 2 Raw Sky Extract 1.0 Ethanol 97.00 Twin 80 2.0 100 Composition 3 Raw Sky Extract 1.0 Water 97.00 Span 80 2.0 100 Composition 4 Raw Sky Extract 1.0 Water 97.00 Lanolin 2.0 100 Composition 5 Dried Sky Blue Extract 1.0 Ethanol 97.00 Lanolin 2.0 100 Composition 6 Dried Sky Blue Extract 1.0 Ethanol 97.00 Lanolin 2.0 100 Composition 7 Dried Sky Blue Extract 1.0 Water 97.00 Lanolin 2.0 100 Composition 8 Dried Sky Blue Extract 1.0 Water 97.0 Lanolin 2.0 100 Control group Composition 9 Raw Sky Extract 1.0 Ethanol 99.00 - 100 Composition 10 Dried Sky Blue Extract 1.0 Ethanol 99.00 - 100

The abdominal skin of a guinea pig was collected in an area of 1㎠ and placed in a permeation cell with a 0.9 cm diameter microscope, and then fixed with a clamp. 0.5 ㎖ of the experimental group and the control group were applied to one side of the skin, and then left at 32℃ for 24 hours. Afterwards, the samples that had penetrated through the applied skin were collected and analyzed by HPLC to measure the degree of absorption into the skin. The results are shown in Table 7 below.

unit Skin absorption level Composition 1 ++ Composition 2 ++ Composition 3 ++ Composition 4 ++ Composition 5 ++ Composition 6 ++ Composition 7 +++ Composition 8 +++ Composition 9 +++ Composition 10 +++

Skin absorption level evaluation: +: Average absorption effect, ++: Fast absorption effect, +++: Very fast absorption effect

Experimental Example 3: Verification of skin moisturizing effect

The skin moisturizing effect of the compositions of Examples 1 to 33 was confirmed as follows using the method for measuring moisturizing effect according to skin type.

Among adult men and women aged 20 years or older, those who met the prescribed selection criteria and did not fall under the exclusion criteria were selected as subjects. Among the subjects, if adverse events such as burning sensation or erythema occurred in the test area during the test, if the subject exposed the test area to excessive ultraviolet rays during the test, or if the evaluation of the results was hindered due to excessive drinking or smoking, or if the subject had difficulty in follow-up observation due to personal reasons during the test, the subject was dropped midway.

Each selected subject applied 2 ml of the composition in the morning and evening to a 5 cm x 5 cm area on the upper arm, which was selected as an area with less sweating and less susceptible to external influences.

The performance evaluation of the composition was conducted by having the subjects' skin stabilize for 30 minutes in a place with no air movement, no direct sunlight, and constant temperature and humidity conditions (20 to 24°C, 40 to 60% RH), and then exposing the area to be measured. After that, the change in skin moisture content was measured three times using a Corneometer to evaluate the effect of the test substance on skin moisture content. In addition, for the evaluation of adverse events, the occurrence of adverse events (erythema, edema, scaling, itching, stinging, burning, tightness, prickling) or other abnormalities was evaluated through questionnaires and visual inspection at each subject's visit in the individual case record form, and the degree was recorded as mild, moderate, or severe. Additionally, we checked whether there were any cases of trial suspension or dropout and recorded them in the CASE REPORT FORM.

Analysis of the results Statistical analysis was performed using statistical processing programs such as SPSS and SAS, and the statistical significance level was set at p<0.05. The results are shown in Table 8 below.

Example Moisturizing effect Example Moisturizing effect Example Moisturizing effect Example 1 ++++ Example 12 +++ Example 23 +++ Example 2 +++ Example 13 +++ Example 24 +++ Example 3 +++ Example 14 +++ Example 25 +++ Example 4 ++++ Example 15 +++ Example 26 +++ Example 5 +++ Example 16 +++ Example 27 +++ Example 6 +++ Example 17 +++ Example 28 +++ Example 7 ++++ Example 18 +++ Example 29 ++++ Example 8 +++ Example 19 +++ Example 30 ++++ Example 9 +++ Example 20 +++ Example 31 ++++ Example 10 ++++ Example 21 +++ Example 32 ++++ Example 11 +++ Example 22 +++ Example 33 ++++

Skin moisturizing effect level evaluation: +: poor, ++: average, +++: good, ++++: very good

Experimental Example 4: Verification of skin anti-inflammatory effect

The skin anti-inflammatory effects of the compositions of Examples 1 to 33 were measured using inflammation-related biomarkers using cell lines, and the results are shown in Table 9 below.

Example Anti-inflammatory effect Example Anti-inflammatory effect Example Anti-inflammatory effect Example 1 ++++ Example 12 ++ Example 23 ++ Example 2 ++ Example 13 +++ Example 24 ++ Example 3 ++ Example 14 + Example 25 ++ Example 4 ++++ Example 15 ++ Example 26 ++ Example 5 + Example 16 ++ Example 27 ++ Example 6 ++ Example 17 +++ Example 28 ++ Example 7 ++++ Example 18 +++ Example 29 ++++ Example 8 ++ Example 19 ++ Example 30 ++++ Example 9 ++ Example 20 ++ Example 31 ++++ Example 10 ++++ Example 21 ++ Example 32 ++++ Example 11 + Example 22 ++ Example 33 ++++

Evaluation of the degree of cell line anti-inflammatory effect: +: slight, ++: moderate, +++: good, ++++: very good

Experimental Example 5: Evaluation of Antioxidant Effect (Anti-aging)

As an evaluation method of experimental raw materials used to suppress aging, such as preventing wrinkles on the skin, the scavenging ability of free radical DPPH was measured by measuring the degree to which the composition of the example scavenged DPPH (2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl radical), a radical, and decolorized, by measuring the absorbance at 565 nm, and the results are shown in Table 10 below.

Free radical scavenging activity(%) Standard material green tea extract
Experimental concentration (0.1%) Sky horse extract
Experimental concentration (0.1%) Experimental group Example 1 18 45 Example 2 18 32 Example 3 18 33 Example 4 18 45 Example 5 18 36 Example 6 18 35 Example 7 18 45 Example 8 18 33 Example 9 18 37 Example 10 18 45 Example 11 18 40 Example 12 18 35 Example 13 18 45 Example 14 18 38 Example 15 18 36 Example 16 18 39 Example 17 18 45 Example 18 18 45 Example 19 18 32 Example 20 18 31 Example 21 18 37 Example 22 18 36 Example 23 18 28 Example 24 18 31 Example 25 18 35 Example 26 18 37 Example 27 18 30 Example 28 18 35 Example 29 18 58 Example 30 18 62 Example 31 18 55 Example 32 18 66 Example 33 18 63

Referring to Table 10, it was confirmed that the compositions of Examples 1 to 33 according to the present disclosure had a very excellent free radical scavenging effect as measured by the DPPH method compared to 0.1% of green tea extract, which is a standard substance.

Experimental Example 6: Analysis of the effective components of the extract of Sky Horse

The effective ingredients and contents of each sample extracted from 1 kg of raw sky yam and 1 kg of dried sky yam dried in the shade for 1 week were measured using analytical equipment such as HPLC, and the results are shown in Tables 11 and 12 below.

Unit: g Musin Saponin Colin Arginine Dietary fiber Diosgenin Example 1 50~700 1.2 0.75 0.37 70~162 0.03 Example 2 420 0.8 0.71 0.41 118 0.01 Example 3 387 0.7 0.70 0.32 125 0.02 Example 4 620 0.9 0.83 0.38 160 0.03 Example 5 460 0.9 0.80 0.35 150 0.03 Example 6 490 0.7 0.75 0.30 148 0.01 Example 7 385 0.5 0.53 0.21 109 0.02 Example 8 368 0.5 0.50 0.19 102 0.01 Example 9 265 0.5 0.51 0.12 107 0.01 Example 10 430 0.3 0.30 0.10 90 0.02 Example 11 360 0.3 0.30 0.09 73 0.01

Unit: g Musin Saponin Colin Arginine Dietary fiber Diosgenin Example 1 50~620 1.1 0.75 0.28 139 0.03 Example 2 320 0.2 0.71 0.09 97 0.01 Example 3 280 0.3 0.70 0.05 98 0.02 Example 4 420 0.7 0.83 0.23 114 0.03 Example 5 260 0.7 0.80 0.21 103 0.03 Example 6 290 0.5 0.75 0.23 96 0.01 Example 7 385 0.4 0.53 0.20 87 0.02 Example 8 268 0.3 0.50 0.10 50 0.01 Example 9 250 0.2 0.51 0.08 38 0.01 Example 10 287 0.3 0.30 0.10 137 0.02 Example 11 250 0.1 0.30 0.05 54 0.01

Although the preferred embodiments of the present disclosure have been described in detail above, the scope of the present disclosure is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present disclosure defined in the following claims also fall within the scope of the present disclosure.

Claims (12)

A functional composition for a cosmetic raw material comprising an extract of Dioscorea bulbifera and plant-derived exosomes, wherein the plant-derived exosomes are exosomes derived from any one plant raw material selected from lotus root, peony flower, okra, and goldenrod. A functional composition for cosmetic raw materials, characterized in that in claim 1, the extract of Dioscorea bulbifera is contained in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is contained in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition. A functional composition for cosmetic raw materials, characterized in that in the second paragraph, the extract of Dioscorea bulbifera (Dioscorea bulbifera) is extracted from dried or raw Dioscorea using at least one solvent selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol. A functional composition for cosmetic raw materials, characterized in that in claim 3, the extract of Dioscorea bulbifera contains 5 to 70 parts by weight of mucin, 0.03 to 0.12 parts by weight of saponin, 0.03 to 0.12 parts by weight of choline, 0.01 to 0.50 parts by weight of arginine, and 0.001 to 0.003 parts by weight of diosgenin, based on 100 parts by weight of the entire Dioscorea bulbifera extract. A functional composition for cosmetic raw materials, characterized in that in claim 4, the functional composition contains carrot seed oil as an additional ingredient, and the carrot seed oil is contained in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition. A functional composition for cosmetic raw materials, characterized in that in claim 5, the functional composition contains an extract of the bark of the bark tree as an additional ingredient, and the extract of the bark of the bark tree is contained in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition. A functional composition for cosmetic raw materials, characterized in that in claim 6, the functional composition contains a senna leaf extract as an additional ingredient, and the senna leaf extract is contained in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition. delete delete delete delete delete