KR102297957B1 - A composition for improving, preventing and treating of acne and atopic dermatitis comprising black garlic extract - Google Patents

Abstract

The present invention relates to a composition for the prevention, improvement and treatment of acne, and by containing black garlic extract as an active ingredient, it improves acne that can be caused by acne causative bacteria and skin cell damage bacteria Propionibacterium acnes, can be prevented or treated. In addition, since it is non-toxic, it can be ingested in the form of food.

Description

A composition for preventing, improving, and treating acne or atopic dermatitis comprising black garlic extract as an active ingredient {A composition for improving, preventing and treating of acne and atopic dermatitis comprising black garlic extract}

The present invention relates to a composition containing black garlic extract as an active ingredient to prevent, improve and treat acne.

The skin is the outermost organ of the human body and accounts for about 7% of the body weight in the case of an adult. In addition, the skin plays a key role in maintaining the normal physiological activity of the human body because it has various functions such as performing an immune response using immune cells, preventing evaporation of water, excreting and excreting wastes, metabolism, and absorption.

However, the skin is also the part of our body where aging is most easily found. Exposure to external environments such as stress, sunlight, air pollution, and tobacco causes reactive oxygen species (ROS) to be generated. , it causes a decrease in fibroblasts and accelerates skin aging.

In addition, as the skin cell activity decreases with age, the ability to produce collagen and elastin decreases. formation is promoted.

Acne is also a chronic inflammatory disease in which inflammation occurs in the sebaceous glands attached to the hair follicles that make hair, as one of the typical problems that occur on the skin. Various skin changes such as comedones, papules, pus blisters, nodules, and pseudocysts appear, and scars may be left as sequelae.

Various causes are known, such as hormonal imbalance, stimulation of hair follicles by acne bacteria, or an inflammatory response. It mainly occurs during puberty, when people are sensitive to appearance, and it is a problem because it can lead to scarring. Various treatment methods and related products for acne have been developed, but there is a need to develop more effective methods or products.

Republic of Korea Patent Publication No. 2019-0010472 Republic of Korea Patent Publication No. 2009-0026446

It is an object of the present invention to provide a pharmaceutical composition capable of preventing or treating acne.

In addition, another object of the present invention is to provide a food composition that can prevent or improve acne.

In addition, another object of the present invention is to provide a cosmetic composition having anti-acne activity.

The pharmaceutical composition for preventing or treating acne of the present invention for achieving the above object may contain black garlic extract as an active ingredient.

The black garlic may be aged for 10 to 30 days by putting garlic and mugwort together in a cypress box.

The garlic and mugwort may be mixed at a weight ratio of 1:0.3-1 and aged at 25 to 35°C.

The black garlic extract can inhibit acne causative bacteria and skin cell damage bacteria, Propionibacterium acnes (KTCT 3314).

The black garlic extract may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The mixed solvent may be an aqueous solution of 60 to 90% by volume or more of methanol or ethanol.

The mixed solvent may be an aqueous ethanol solution of 60 to 90% by volume or more.

In addition, the food composition for preventing or improving acne of the present invention for achieving the above other object may contain black garlic extract as an active ingredient.

The black garlic may be aged for 10 to 30 days by putting garlic and mugwort together in a cypress box.

The garlic and mugwort may be mixed at a weight ratio of 1:0.3-1 and aged at 25 to 35°C.

The black garlic extract can inhibit acne causative bacteria and skin cell damage bacteria, Propionibacterium acnes (KTCT 3314).

In addition, the cosmetic composition having anti-acne activity of the present invention for achieving the above another object may contain black garlic extract as an active ingredient.

The composition for the prevention, improvement and treatment of acne containing the black garlic extract of the present invention as an active ingredient is very effective in improving, preventing or treating acne, and thus is effective in the preparation of competitive food compositions, pharmaceutical compositions and cosmetic compositions.

In addition, since the composition of the present invention is non-toxic, it can be ingested in the form of food.

1 is a _{graph and IC 50} values showing the biofilm inhibitory activity of samples for each aging period for P. acnes.
Figure 2 is a graph measuring the enzyme inhibition of the sample for each aging period for P. acnes.
3 is a Western blot showing the inhibition of inflammation according to the black garlic extract for each ripening date prepared according to the example.
Figure 4a is a graph showing the ABTs radical scavenging ability of the extracts prepared in Examples and Comparative Examples, Figure 4b is a graph showing _{the EC 50 value of Figure 4a.}
Figure 4c is a graph showing the DPPH radical scavenging ability of the extracts prepared in Examples and Comparative Examples, Figure 4d is a graph showing _{the EC 50 value of Figure 4c.}
5 is a Western blot measuring Akt and ERK signaling systems according to the 25-day ripening black garlic extract according to the Example and the extract of Comparative Example 1. FIG.
Figure 6 is a Western blot measuring the Akt and ERK signaling systems according to the concentration of the extract of the 25th day of aging according to the Example and the concentration of the extract of Comparative Example 1.

The present invention relates to a composition containing black garlic extract as an active ingredient to prevent, improve and treat acne.

The composition of the present invention can be used for various purposes and uses that require anti-acne or antioxidant activity, and specifically, a function capable of imparting antioxidant activity to articles applied in various industrial fields such as pharmaceuticals, cosmetics, food and animal feed. It can be used as a material, and can also be used as a material for pharmaceutical preservatives, cosmetic preservatives, food preservatives, pharmaceutical additives, cosmetic additives, food additives and feed additives.

Hereinafter, the present invention will be described in detail.

The composition capable of preventing, improving or treating acne of the present invention contains black garlic extract as an active ingredient.

The black garlic has more sugar than raw garlic, acidity is generated, and has properties similar to jelly, so that the amount of black garlic that can be consumed as it is compared to raw garlic is high, and the sensual preference is higher. However, these characteristics may cause difficulties in secondary processing, and when the drying method, which is the most commonly used processing method, is used, the sugar in garlic makes drying difficult or excessively hard, so it is not suitable. In addition, it is known that black garlic has a high antioxidant activity as a browning reaction material generated during the aging process, and has effects related to cancer prevention, cholesterol lowering, arteriosclerosis improvement, heart disease prevention, and oxidative stress.

The black garlic of the present invention is prepared by putting garlic and mugwort together in a cypress box in a weight ratio of 1:0.3-1, preferably 1:0.5-0.8, and aging at 25 to 35° C. for 10 to 30 days. It is aged together with mugwort in a cypress box so that the aroma of mugwort is infused into the garlic, and it kills bacteria and other germs on the surface of garlic, sterilizing, antibacterial and deodorizing, as well as Propionibacterium arc, a bacteria that causes acne and damages skin cells. It was confirmed that the antibacterial activity against Ness (Propionibacterium acnes, KTCT 3314) was excellent.

If the content of mugwort based on the garlic is less than the lower limit, the antibacterial activity against Propionibacterium acnes (KTCT 3314) may be low, and if it exceeds the upper limit, acne may be rather induced. .

In addition, when the number of days of aging and the temperature are out of the above ranges, the desired effect was obtained.

The black garlic extract of the present invention is first extracted and filtered at 30 to 60 ° C., preferably 40 to 50 ° C. for 8 to 15 hours, preferably 10 to 13 hours, and then the residue is dried at the same temperature for 8 to 15 hours; Preferably, the secondary extraction is performed for 10 to 13 hours, and the primary extract and the secondary extract are mixed and used. If the extraction is performed only once, antibacterial activity against Propionibacterium acnes (KTCT 3314) may be low.

The extraction solvent is not particularly limited, but water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be mentioned, and the extract extracted with 60 to 90% by volume or more of an aqueous ethanol solution is extracted with water or other organic solvents. Antibacterial activity against Propionibacterium acnes (KTCT 3314) is superior to that of the extract.

The black garlic extract prepared as described above may be used as the extract itself or powdered for convenient use. The method of pulverizing the black garlic extract is powdered by concentrating the extract under reduced pressure to reduce the volume and then freeze-drying it with a freeze dryer.

In a broad sense, the black garlic extract of the present invention has a meaning including black garlic processed products formulated so that the black garlic extract can be administered to animals, for example, black garlic powder. Although the experiment was conducted with black garlic extract in the present invention, it will be expected by those skilled in the art that the desired effect can be achieved even in the same form as black garlic processed products.

Meanwhile, in the present specification, the term 'contained as an active ingredient' means including an amount sufficient to achieve the efficacy or activity of the black garlic extract. For example, the mixture containing black garlic is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. Since the mixture containing black garlic is a natural product and there is no side effect to the human body even when administered in excess, the upper limit of the quantity of the mixture containing black garlic included in the composition of the present invention can be selected and carried out by those skilled in the art within an appropriate range.

The pharmaceutical composition of the present invention may be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant includes an excipient, a disintegrant, a sweetener, a binder, a coating agent, a swelling agent, a lubricant, A lubricant or flavoring agent may be used.

The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration.

Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectables. For formulation in the form of, for example, tablets or capsules, the active ingredient may be combined with an orally, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or required, suitable binders, lubricants, disintegrants and color-developers may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

In the composition formulated as a liquid solution, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets.

Furthermore, by using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by an appropriate method in the art, it can be preferably formulated according to each disease or component.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration. .

A suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration mode, age, weight, sex, morbidity, food, administration time, administration route, excretion rate and response sensitivity of the patient, An ordinarily skilled physician can readily determine and prescribe a dosage effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dose of the pharmaceutical composition of the present invention is 0.001-10 g/kg.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person of ordinary skill in the art to which the present invention pertains. or may be prepared by incorporation into a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, or emulsion in oil or an aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersant or stabilizer.

In addition, the present invention provides a food composition capable of preventing, treating or improving acne containing black garlic extract as an active ingredient.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, sweets, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gums, ice cream, vitamin complexes, health supplements etc.

The food composition of the present invention may include not only a mixture containing black garlic extract as an active ingredient, but also ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. includes Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink and beverage, citric acid, high fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts, etc. are additionally included in addition to the mixture containing the black garlic extract of the present invention. can do it

The present invention provides a health functional food comprising a food composition for preventing, treating or improving acne comprising the black garlic extract as an active ingredient. A health functional food is a food prepared by adding a mixture containing black garlic extract to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspension, etc. However, unlike general medicines, it has the advantage that there are no side effects that may occur when taking medicines for a long period of time by using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. The amount of black garlic extract added in such a health functional food varies depending on the type of health functional food and cannot be uniformly defined. to 50% by weight, preferably 0.1 to 20% by weight. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention provides the use of the black garlic extract for the manufacture of a medicament or food for the prevention, treatment or improvement of acne. As described above, the black garlic extract can be used for acne.

In addition, the present invention provides a method for improving, preventing or treating acne, comprising administering to a mammal an effective amount of a black garlic extract.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

As used herein, the term “effective amount” refers to the amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human as conceived by a researcher, veterinarian, physician or other clinician, which an amount that induces amelioration of the symptoms of a disease or disorder. It is apparent to those skilled in the art that the effective amount and frequency of administration for the active ingredient of the present invention will vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and the type of disease, the severity of the disease, the content of the active ingredient and other components contained in the composition, the type of formulation, and the age, weight, and general health of the patient It can be adjusted according to various factors including condition, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, and concurrently used drugs. In the prevention, treatment or improvement method of the present invention, for adults, it is preferable to administer milk thistle and onion once to several times a day, at a dose of 0.001 g/kg to 10 g/kg.

In the treatment method of the present invention, the composition comprising black garlic extract as an active ingredient is administered in a conventional manner through oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. can be administered.

In addition, in the cosmetic composition of the present invention, other ingredients commonly blended in cosmetics may be blended as necessary in addition to the cosmetic composition described above, and as such blended ingredients, water, oil, surfactant, humectant, thickener, fragrance, preservative, and at least one selected from the group consisting of a neutralizer, an emollient, a skin protectant, and a skin conditioning agent.

In addition, the cosmetic composition may be prepared in the form of an emulsion formulation and a solubilization formulation commonly used in the art, for example, skin, emulsion, essence, lotion, serum, body lotion, body gel, body essence, body cleaner, cleansing Any one formulation selected from the group consisting of foam, cleansing cream, cleansing gel, pack, massage cream, massage gel, nourishing cream, sleeping cream and moisture cream may be used.

Hereinafter, preferred examples are presented to aid the understanding of the present invention, but the following examples are merely illustrative of the present invention and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example. Black Garlic Extract

Garlic and mugwort were mixed in a weight ratio of 1:0.6, put in a cypress box, and aged for 25 days per day. Then, 100 g of aged black garlic was mixed with 500 ml of 70% ethanol aqueous solution for 12 hours in a shaking water bath at 40 ° C. After extraction and filtration, the residue was subjected to secondary extraction with 500 ml of 70% ethanol aqueous solution in a shaking constant temperature water bath at 40° C. for 12 hours, and the primary and secondary extracts were mixed. After evaporating the mixed extracts, black garlic extract powder was obtained using a freeze dryer.

The 5th day of aging was indicated as G-5, the 6th day of aging was indicated as G-6, and the 7th day of aging was indicated as G-7.

Comparative Example 1. Garlic Extract

It was carried out in the same manner as in Example 1, except that ordinary garlic was used instead of black garlic to obtain a garlic extract powder (G-0).

<Test Example>

bacterial culture

Propionibacterium acnes (KTCT 3314) The strain was obtained from the Korea Center for Biological Resources (Jeongeup-si, Jeollabuk-do) and cultured in Brain heart infusion broth (BHI) supplemented with 1% glucose. After culturing the strain at 37 °C under anaerobic conditions for 48 hours, the culture was subcultured and cultured at 37 °C under anaerobic conditions for 72 hours. Cultures were made in 15% glycerol solution and maintained at -80°C until use.

Test Example 1. Measurement of antibacterial activity

Disc diffusion analysis was used to screen the antibacterial activity of black garlic extract. P. acnes at a concentration of about 10 ⁸ CFU/ml was inoculated on Muller Hilton agar (MHA) at a concentration of 1/50 and poured onto the surface of a pre-prepared MHA petri dish. A sterile disk (Ⅸ 8 mm) was placed on the MHA, and 40 μl of the extract (200 mg/ml) was dispensed on the disk.

Classification (Sample Concentration: 8 mg/disc) Inhibition zone (mm±SD) Comparative Example 1 G-0 8.2±0.4 Example G-5 9.9±0.5 G-7 9.8±0.5 G-10 10.9±0.3 G-11 11.6±0.3 G-12 11.6±0.4 G-13 11.2±0.5 G-14 11.5±0.6 G-17 11.0±0.3 G-19 11.5±0.5 G-21 11.6±0.5 G-23 11.3±0.7 G-25 11.5±0.6

As shown in Table 1 above, it was confirmed that the black garlic extract prepared according to the example of the present invention had superior antibacterial activity compared to the garlic extract of Comparative Example 1. In particular, in the aged black garlic after the 11th day of aging (G-11), it was confirmed that the suppression area had a diameter of 11 mm or more, so that the antibacterial activity was excellent.

That is, it was confirmed that black garlic had antibacterial activity against acne mycelium compared to the raw material at a sample concentration of 8 mg/disc.

Test Example 2. Measurement of anti-biofilm activity

Biofilm formation was assessed using crystal violet assay. P. acnes ^{at a concentration of about 10 7} CFU/ml cultured in BHI 1% glucose medium was inoculated into a 96 well plate, and cultured for 4 hours in an incubator at 37° C. under anaerobic conditions to attach. The inoculum was removed and washed with PBS solution to remove non-adherent bacteria. After washing, fresh medium was added and incubated for 72 hours under anaerobic conditions to form a biofilm.

1 is a _{graph and IC 50} values showing the biofilm inhibitory activity of samples for each aging period for P. acnes.

As shown in FIG. 1 , it was confirmed that the black garlic extract prepared according to the example of the present invention had a higher inhibitory activity than the garlic extract of Comparative Example 1 and thus the P. acnes survival rate was low.

Antibiotic film activity against acne mycelium ( P. acnes _{) between each sample was expressed as IC 50} (Half maximal inhibitory concentration) value. The IC ₅₀ value decreased significantly from the 11th day of aging (G-11) and the 21st day of aging (G-21). The lowest IC ₅₀ value was 0.34 mg/ml on the 23rd (G-23) and 25th (G-25) days of aging.

Test Example 3. Acne mycelium ( P. acnes ) to measure enzyme inhibition of

The secreted enzyme activity of the acne mycelium ( P. acnes ) was compared with the garlic raw material of Comparative Example 1 (G-0), the 11th day of aging (G-11), the 12th day of aging (G-12), the 25th day of aging (G-25) It was evaluated using extracts. As a control for protease production, 2% casein was added, and as a control for lipase production, a normal agar medium supplemented with 1% tributyrin was used. 1% tributyrin and 2% casein to which the extract is added at a concentration of 1 mg/ml Place a sterilized disc on a normal agar medium and remove P. acnes 10 μl of each was aliquoted and cultured under anaerobic conditions for 72 hours. Enzyme activity inhibition was analyzed by comparison with the diameter of the control group.

Acne mycelium ( P. acnes ) produces digestive enzymes including lipase and protease, which can cause inflammatory acne and damage to skin cells. Lipase generated from the acne mycelium can be converted into free fatty acids that cause skin irritation through metabolism in sebum, and protease can induce inflammatory IL-1b production by activating Nod-like receptors in sebocytes (Wunnoo, S., et al, 2017).

Figure 2 is a graph measuring the enzyme inhibition of the sample for each aging period for P. acnes.

As shown in FIG. 2 , it was confirmed that the black garlic extract prepared according to the example of the present invention inhibited Protease and lipase, which are enzymes produced by the acne mycelium ( P. acnes ), compared to the extract of Comparative Example 1.

Specifically, as a result of comparing the 11th day (G-11), the 12th day (G-12), the 25th day (G-25) of maturation of black garlic extract and the garlic raw material (G-0), the effect of inhibiting lipase secretion was also matured. It was confirmed that it decreased significantly according to the period, and the inhibitory effect of protease secretion also showed that the degree of hydrolysis was significantly decreased from the raw material to the 25th day (G-25). was confirmed to be In particular, on the 25th day of aging (G-25), the enzyme hydrolysis rate was reduced by about 95% compared to the control to 5.3%.

Test Example 4. Measurement of Inflammation Inducing Efficacy

HaCaT cells (human skin cells) were seeded in a dish and cultured in a culture dish of 70-80%, followed by fasting for 24 hours using a medium not containing FBS, then treated with black garlic extract for 1 hour, and acne mycelium (P). .acnes) was treated for 6 hours, the protein was recovered using lysis buffer, and then the protein was analyzed using Western blot test.

3 is a Western blot showing the inhibition of inflammation according to the black garlic extract for each ripening date prepared according to the example.

As shown in FIG. 3 , anti-inflammatory effects were observed in the extracts on the 11th day (G-11) and the 25th day (G-25) of aging for the expression of COX-2 increased by the acne mycelium treatment.

Test Example 5. Measurement of antioxidant activity

Antioxidant activity was calculated through DPPH and ABTs radical scavenging activity. DPPH radical scavenging activity was measured by dissolving samples of various concentrations in DMSO, mixing 100 μl of the DPPH solution and 100 μl of each sample, and measuring the absorbance at 517 nm for the mixed sample using a spectrophotometer. The radical scavenging activity of ABTs was obtained by mixing 7 mM ABTs solution and 2.4 mM potassium persulfate, reacting in a dark place at refrigeration temperature for 12 to 16 hours, and diluting so that the absorbance at 415 nm optical density was 1.5. The absorbance was measured at 415 nm optical density by mixing 50 μl of the diluted sample for each concentration and 150 μl of the prepared ABTs solution. Ethanol was used as a control and calculated as the ratio of the absorbance of each sample to the control.

Figure 4a is a graph showing the ABTs radical scavenging ability of the extracts prepared in Examples and Comparative Examples, Figure 4b is a graph showing _{the EC 50 value of Figure 4a.} Figure 4c is a graph showing the DPPH radical scavenging ability of the extracts prepared in Examples and Comparative Examples, Figure 4d is a graph showing _{the EC 50 value of Figure 4c.}

As shown in Figures 4a to 4d, the longer the aging period in ABTs radical scavenging activity and DPPH radical scavenging activity, the higher the antioxidant activity, the lower the EC ₅₀ (Half maximal effective concentration) value was confirmed.

Specifically, EC ₅₀ values are aged 23 primary (G-23) in ABTs (19 mg / ml) and was lowest both DPPH (40 mg / ml), according to the result of aging period check the EC ₅₀ for all samples EC _{The 50} value decreased significantly. As a result, it was shown that the black garlic extract had higher antioxidant activity as the aging period increased.

Test Example 6. Inhibition of inflammation specific signal transduction system

After the protein was recovered in the same manner as in Test Example 4, phosphorylation levels of Akt and ERK were measured.

5 is a Western blot measuring Akt and ERK signaling systems according to the 25-day ripening black garlic extract according to the Example and the extract of Comparative Example 1. FIG.

As shown in FIG. 5 , it was confirmed that the Akt and ERK signaling systems were increased by the acne mycelium, and the black garlic extract of the 25th day of aging (G-25) inhibited it.

Figure 6 is a Western blot measuring the Akt and ERK signaling systems according to the concentration of the extract of the 25th day of aging according to the Example and the concentration of the extract of Comparative Example 1.

As shown in FIG. 6 , it was confirmed that the Akt and ERK signaling systems were increased by acne mycelium and that the black garlic extract on the 25th day of aging (G-25) inhibited it in a concentration-dependent manner.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe it in detail.

Formulation Example 1. Preparation of powder

500 mg of extract powder obtained in Example 1

Lactose 100 mg

talc 10 mg

The above ingredients are mixed and filled in an airtight bag to prepare a powder.

Formulation Example 2. Preparation of tablets

300 mg of extract powder obtained in Example 1

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 3. Preparation of capsules

200 mg of extract powder obtained in Example 1

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 4. Preparation of injection

600 mg of extract powder obtained in Example 1

mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO _4, 12H ₂ O 26 mg

According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule is prepared.

Formulation Example 5. Preparation of liquid formulation

4 g of extract powder obtained in Example 1

10 g isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to a conventional liquid preparation method, each component is added to purified water to dissolve, an appropriate amount of lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100 g by adding purified water, and then filled in a brown bottle and sterilized to prepare a liquid.

Formulation Example 6. Preparation of granules

1,000 mg of extract powder obtained in Example 1

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

1.7 mg of nicotinic acid amide

50 μg folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

potassium phosphate monobasic 15 mg

Dicalcium Phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules in a preferred embodiment, but the mixing ratio may be arbitrarily modified. It can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

1,000 mg of extract powder obtained in Example 1

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Add purified water to total 900 mL

After mixing the above ingredients according to the usual health drink manufacturing method, after stirring and heating at 85 ° C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demanding country, and use.

In addition, formulation examples of the cosmetic composition suitable for applying the present invention will be presented.

Formulation example 8. lotion

Preparation examples of the lotion among the cosmetics containing the extract of Example 1 are shown in Table 2 below.

ingredient content (wt%) Extract of Example 1 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PG 1500 1.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Formulation Example 9. Lotion

Preparation examples of the lotion in the cosmetic containing the extract of Example 1 are shown in Table 3 below.

ingredient content (wt%) Extract of Example 1 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 Macadamina Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Formulation Example 10. Nutritional Cream

A preparation example of a nutritional cream in a cosmetic comprising the extract of Example 1 is shown in Table 4 below.

ingredient content (wt%) Extract of Example 1 1.0 lipophilic monostearate glycerin 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearylisostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Formulation Example 11. Essence

Preparation examples of the essence in the cosmetic containing the extract of Example 1 are shown in Table 5 below.

ingredient content (wt%) Extract of Example 1 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 Sodium Hyaluronate 8.0 Carboxyvinyl Polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Formulation Example 12. Emulsion for Mask Pack

Preparation examples of the emulsion for a mask pack among the cosmetics containing the extract of Example 1 are shown in Table 6 below.

ingredient content (wt%) Extract of Example 1 1.0 polyvinyl alcohol 15.0 Cellulose Gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-Panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Claims (12)

Contains black garlic extract as an active ingredient,
The black garlic is a pharmaceutical composition for preventing or treating acne, characterized in that it is aged for 10 to 30 days at 25 to 35 ℃ by adding garlic and mugwort together in a weight ratio of 1: 0.3-1 in a cypress box. delete delete [Claim 3] The pharmaceutical composition for preventing or treating acne according to claim 1, wherein the black garlic extract inhibits Propionibacterium acnes (KTCT 3314), which is an acne causative bacteria and a skin cell damage bacterium. The pharmaceutical composition for preventing or treating acne according to claim 1, wherein the black garlic extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The pharmaceutical composition for preventing or treating acne according to claim 5, wherein the mixed solvent is an aqueous solution of 60 to 90% by volume or more of methanol or ethanol. The pharmaceutical composition for preventing or treating acne according to claim 5, wherein the mixed solvent is an aqueous solution of 60 to 90% by volume or more of ethanol. Contains black garlic extract as an active ingredient,
The black garlic is a food composition for preventing or improving acne, characterized in that it is aged for 10 to 30 days at 25 to 35 ℃ by adding garlic and mugwort together in a weight ratio of 1: 0.3-1 in a cypress box. delete delete The food composition for preventing or improving acne according to claim 8, wherein the black garlic extract inhibits Propionibacterium acnes (KTCT 3314), which is an acne-causing bacterium and a skin cell damaging bacterium. Contains black garlic extract as an active ingredient,
The black garlic is a cosmetic composition having anti-acne activity, characterized in that the garlic and mugwort are put together in a cypress box in a weight ratio of 1:0.3-1 and aged at 25 to 35° C. for 10 to 30 days.

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