KR102158134B1 - Antibacterial composition comprising an extract of schisandra chinesis - Google Patents

Abstract

The present invention relates to an antibacterial composition containing Schisandra chinensis extract as an active ingredient, and more particularly, it contains Schisandra chinensis extract, a natural raw material, and can be applied as an antibacterial composition that can be safely used in the human body without toxicity and side effects.

Description

Antibacterial composition comprising an extract of schisandra chinesis}

The present invention relates to an antimicrobial composition containing the Schisandra chinensis extract as an active ingredient, and more particularly, to a technology for preparing an antibacterial composition that can be safely used in the human body without toxicity and side effects by containing Schisandra chinensis extract, a natural raw material. will be.

Direct or indirect damage caused by pathogenic microorganisms causes many economic, environmental and medical problems. Many problems in society, such as loss due to spoilage during the food distribution process in the food industry, harmfulness and environmental pollution to the human body due to the use of excessive chemical pesticides on agricultural products, and the emergence of antibiotic-resistant strains due to misuse of antibiotics. Are causing them.

Vancomycin-resistant staphylococcus aureus (VRSA), which is highly resistant to vancomycin-resistant staphylococcus aureus, known as the last treatment for Staphylococcus aureus, the most common cause of human infection, was reported for the first time in the world by the Centers for Disease Control in 2002. As a result, the possibility of the spread of so-called super bacteria is very high. This is the first time in Europe where methicillin-resistant staphylococcus aureus (MRSA), which is treated only by vancomycin, was a problem since the 1970s. As a new example of antibiotic resistance, which emerged as a global crisis in the late 1990s, when vancomycin intermediate-resistant s. aureus (VISA), which is resistant to vancomycin, reported in Japan, the United States, France, and Korea occurred in the late 1990s. As it shows how serious the resistance problem is, the development of new antibiotics is urgently required (Non-Patent Document 1; Non-Patent Document 2).

Most of the antibiotics currently used are manufactured through chemical synthesis, and are expensive and have many limitations, such as causing side effects. Therefore, in recent years, research to separate new antibacterial substances from natural products has been actively conducted. In order to separate new antimicrobial substances from natural products, it is necessary to consider the fact that the antimicrobial spectrum is broad and safe without side effects even after long-term administration.

In recent years, as interest in natural extracts increases, research on various plants is being actively conducted, and as antibacterial effects are known, many plant extracts are applied to antibacterial compositions.

On the other hand, Omija (五味子, Schizandra chinensis Baillon.) is a deciduous broad-leaved vine, its leaves are alternated, ovate or obovate, and the tip is sharp and serrated, and there are some hairs on the veins on the back side. Flowers are red and white, bloom from June to July, and are hermaphrodite. Fruits are used for edible purposes, hang in a spike shape at the end of a branch, and ripen red in August-September. The fruits are called Schizandrae fructus and Schizandrae fructus. Altitude 200~1,600 m Jirisan, Jeonbuk, and Gangwon-do are the main production areas, wild throughout the country except Chungnam and Chungbuk, and geographically distributed in Japan, Sakhalin, Manchuria, and China. Fruits include: Schizandrol, Schizandrin A, B, C, angeloylgomisin O, P, epigomisin, pregomoisin, deoxyschizandrin. ), etc. Schisandra chinensis has long been used in herbal medicine as a raw material for herbal medicines with medicinal effects such as converging, nourishment, tonic, Jinhae medicine, Haejudok, thirst, sorghum gosap, ikgi saengjin, and bosin yeomsim. It has been used as a raw material for schisandra. The safety of the human body by ingestion has already been confirmed.

Therefore, the present inventors have come to complete the present invention by conceiving that if the Schisandra chinensis extract can be prepared, it can be used as an antibacterial composition that can be safely used without toxicity and side effects.

Non-Patent Documents 1. Pfeltz, R. F., and B. J. Wilkinson. Current Drug Targets-Infectious Disorders 4.4 (2004): 273-294. Non-Patent Document 2. Levy, Stuart B., and Bonnie Marshall. Nature medicine 10 (2004): S122-S129.

The present invention has been conceived in consideration of the above problems, and an object of the present invention is to provide an antibacterial composition that can be safely used on the human body without toxicity and side effects by containing the extract of Schisandra chinensis, a natural raw material.

The antimicrobial composition of the present invention for achieving the above object may contain Schisandra chinensis extract as an active ingredient.

The Schisandra chinensis extract may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The Schisandra chinensis extract may be a hot water extract.

The Schisandra may be a pulp of Schisandra.

The Schisandra chinensis extract is Staphylococcus aureus, Staphylococcus epidermidis, methicillin-resistant S. aureus, quinolones-resistant Staphylococcus aureus, and Aki. It can exhibit antimicrobial activity against one or more microorganisms selected from the group consisting of Acinetobacter calcoaceticus, Micrococcus luteus, Bacillus subtilis, and Streptococcus mutans. have.

In addition, the antimicrobial pharmaceutical composition of the present invention for achieving the other object described above may contain Schisandra chinensis extract as an active ingredient.

In addition, the antimicrobial cosmetic composition of the present invention for achieving the other objects described above may contain Schisandra chinensis extract as an active ingredient.

The cosmetic composition may be in the form of a solution, powder, emulsion, lotion, spray, ointment, aerosol, cream, or foam.

In addition, the antimicrobial food additive of the present invention for achieving the other object described above may contain Schisandra chinensis extract as an active ingredient.

In addition, the antimicrobial feed additive of the present invention for achieving the other object described above may contain Schisandra chinensis extract as an active ingredient.

In addition, the antimicrobial hygiene product of the present invention for achieving the other object described above may contain the Schisandra chinensis extract as an active ingredient.

According to the present invention, it is possible to provide an antibacterial composition that can be safely used in the human body without toxicity and side effects by containing the extract of Schisandra chinensis, a natural raw material.

1 is an image showing a process in which the Schisandra chinensis extract prepared from Examples 1 and 2 of the present invention is prepared.
2 is an image of a result of measuring the antimicrobial activity of Schisandra chinensis extracts prepared from Examples 1 and 2 of the present invention by concentration by disc diffusion assay.

Hereinafter, various aspects and various embodiments of the present invention will be described in more detail.

The present invention relates to a technology for preparing an antibacterial composition that can be safely used on the human body without toxicity and side effects by containing the extract of Schisandra chinensis, a natural raw material.

In the present invention, "antibacterial" refers to an effect against bacteria, and more specifically, an action of inhibiting the growth of bacteria and fungi by a drug or a physiologically active substance.

In particular, the Schisandra chinensis extract according to the present invention includes Staphylococcus aureus, Staphylococcus epidermidis, Methicillin-resistant S. aureus, and Quinolone-resistant Staphylococcus aureus (Quinolone-resistant S. aureus), Acinetobacter calcoaceticus, Micrococcus luteus, Bacillus subtilis, and Streptococcus mutans against at least one microorganism selected from the group consisting of It is preferable to exhibit antimicrobial activity, but is not particularly limited to the above type of strain.

Hereinafter, various aspects and various embodiments of the present invention will be described in more detail.

The antimicrobial composition of the present invention may contain Schisandra chinensis extract as an active ingredient.

The Schisandra chinensis extract may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. At this time, the lower alcohol is not particularly limited, but may be 20 to 100% by volume of methanol, ethanol, propanol, or butanol.

In the present invention, it is preferable to use Schisandra chinensis extract extracted with hot water using water as an extraction solvent, and it is preferable to use the pulp of Schisandra chinensis.

Two methods for preparing the Schisandra chinensis hot water extract are presented as follows.

In the first method, the dried Schisandra chinensis is obtained by washing with distilled water and drying it. Next, 5 to 15 times, preferably 6 to 12 times, more preferably 8 to 10 times the weight of purified water is added to the dried Schisandra chinensis flesh, and 30 to 90°C, preferably 40 to 80°C, more preferably Schisandra chinensis extract can be prepared by hot water extraction at 50 to 70° C. for 1 to 5 hours, preferably 2 to 4 hours.

In the second method, the Schisandra chinensis is washed with distilled water and dried to obtain dried Schisandra chinensis. Next, 5 to 15 times, preferably 6 to 12 times, more preferably 8 to 10 times the weight of purified water is added to the pulverized Schisandra chinensis pulverized product, and 30 to 90°C, preferably 40 to Hot water extraction at 80° C., more preferably 50 to 70° C., preferably 6 to 12 hours, preferably 7 to 9 hours, can be performed to prepare a hot water extract of Schisandra chinensis.

The Schisandra chinensis extract may be prepared further comprising filtering and concentrating each of the prepared hot water extracts.

When the weight ratio between the extraction solvent and Schisandra chinensis during the extraction is out of the above range, the physiological activity effect of the Schisandra chinensis component may be lowered.

In addition, if the extraction temperature is out of range, antibacterial efficacy may be significantly reduced.

The Schisandra chinensis extract of the present invention is used in a concentration of 0.001 to 20 mg/ml. If the concentration is less than the lower limit, antibacterial efficacy cannot be expected, and if it exceeds the upper limit, toxicity may occur.

The Schisandra chinensis extract prepared as described above may be used as an extract itself, or may be powdered for convenient use. The method of powdering the Schisandra chinensis extract is to reduce the volume by concentrating the extract under reduced pressure, and then powdering the extract by lyophilization, spray drying, and hot air drying.

The antimicrobial composition containing the Schisandra chinensis extract of the present invention as an active ingredient comprises 0.001 to 20% by weight, preferably 0.01 to 10% by weight, based on the total weight of the pharmaceutical composition.

The antibacterial pharmaceutical composition according to another feature of the present invention comprises the Schisandra chinensis extract according to the present invention as an active ingredient.

The method of administering the pharmaceutical composition is not particularly limited, but is preferably injected into an artery or vein, or subcutaneously, rectal, nasal, or any other parenteral administration, and more preferably, arterial or It is preferred to be administered intravenously, orally, or directly into muscle cells.

In addition, the dosage level selected from the composition will depend on the activity of the compound, the route of administration, the severity of the condition being treated and the condition and previous medical history of the patient being treated. However, it is within the knowledge of the art to start with a dose of the compound at a lower level than required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved, and the preferred dosage is age, sex. , It can be determined according to body type and weight. The composition may be further processed prior to being formulated into a pharmaceutically acceptable pharmaceutical formulation, preferably pulverized or ground into smaller particles. Also, the composition will depend on the condition and the patient being treated, but this can be determined non-ingeniously. For a desirable effect, the effective dose of the Schisandra chinensis extract of the present invention is 1 to 2 mg/kg, preferably 0.5 to 1 mg/kg, and may be administered 1 to 3 times a day. The above dosage does not in any way limit the scope of the present invention.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person having ordinary knowledge in the art. Or it can be made by incorporating it into a multi-dose container. At this time, the dosage form can be used in any form suitable for pharmaceutical preparations, including oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations such as ointments and creams, suppositories and sterile injectable solutions, etc. , A dispersant or a stabilizer may additionally be included.

In addition, the Schisandra chinensis extract is Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus mutans, Propionibacterium acnes, E. coli D31, E. coli ML35p, Shigella flexneri, Pseudomonas aeruginosa, Vibrio parahaemolyticus or Candida albicans (Candida) albicans), since it is possible to prevent or treat diseases such as food poisoning, candism, typhoid fever, cholera, etc., which are representative diseases from), the Schisandra chinensis extract is included as an active ingredient to prevent or treat infectious diseases caused by the bacteria. It can be used as a pharmaceutical composition.

In addition, the Schisandra chinensis extract may be used as an active ingredient in an antibacterial cosmetic composition. At this time, the cosmetic composition may be in the form of a solution, powder, emulsion, lotion, spray, ointment, aerosol, cream, or foam. Further, in the cosmetic composition of the present invention, a carrier acceptable in a cosmetic formulation may be included. Herein, “acceptable carrier in cosmetic preparations” refers to a compound or composition that is already known and used that can be included in a cosmetic preparation, or is a compound or composition to be developed in the future. Say nothing.

The carrier may be included in the cosmetic composition of the present invention in an amount of about 1% to about 99.99% by weight, preferably about 90% to about 99.99% by weight, based on the total weight of the cosmetic composition. Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizing agents, viscosity modifiers, emulsions, stabilizers, ultraviolet scattering agents, ultraviolet absorbers, coloring agents, fragrances, and the like. Compounds or compositions that can be used as alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizing agents, viscosity modifiers, emulsions, stabilizers, ultraviolet scattering agents, ultraviolet absorbers, coloring agents, fragrances, etc. are already known in the art. Therefore, those skilled in the art can select and use an appropriate corresponding material or composition.

As an embodiment of the present invention, the cosmetic composition according to the present invention may contain glycerin, butylene glycol, propylene glycol, polyoxyethylene hydrogenated castor oil, ethanol, triethanolamine, etc., in addition to the peptide analogues, and preservatives, fragrances, Colorants, purified water, etc. may be included in trace amounts as needed.

The skin in the present invention is a concept that includes not only the face, but also the scalp and the whole body, and as a cosmetic composition that can be applied to such scalp, there are shampoos, conditioners, treatments, hair growth agents, etc., and a body cleanser that can be applied to the whole body. It can be manufactured in various forms for use.

In addition, the Schisandra chinensis extract may be used as an active ingredient of an antibacterial food additive or a feed additive.

In a specific embodiment of the present invention, the Schisandra chinensis extract according to the present invention has excellent antimicrobial activity against not only Gram-negative and Gram-positive bacteria, but also fungi, and has no cytotoxicity, so it can be usefully used as an antibacterial food additive or feed additive.

There is no particular limitation on the type of the food. Examples of foods to which the above substances can be added include drinks, meat, sausages, bread, biscuits, rice cakes, chocolates, candies, snacks, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, There are alcoholic beverages and vitamin complexes, and all health functional foods in the usual sense are included. The Schisandra chinensis extract of the present invention may be added to food as it is or may be used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (for prevention or improvement). In general, the amount of the Schisandra chinensis extract in the food may be added in an amount of 0.1 to 20% by weight of the total food weight. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of health control, the amount may be below the above range, and there is no problem in terms of safety, so the active ingredient may be used in an amount above the above range. .

In addition, the Schisandra chinensis extract can be usefully used as an active ingredient in hygiene products such as wet tissues, hand sanitizers, mouthwashes, mouth disinfectants, and toothpaste additives.

Hereinafter, the present invention will be described in detail with reference to preferred embodiments so that those of ordinary skill in the art can easily implement the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein.

<Reference example>

Reference Example 1: Culture of strain

For P.acnes, GAM ("Nissui" Gifu anearobic medium) medium was used, and the bacteria were inoculated in a culture medium, sealed in an anaerobic jar, and anaerobic cultured at 37°C for 48 hours.

S.aureus and S.epidermidis was used as an NB (Nutrient Broth Difdo ^TM) medium was used to culture at 37 ℃ after inoculation with the bacteria for 24 hours.

<Example>

Example 1: Preparation of finely ground Schisandra chinensis pulp hot water extract

Schisandra chinensis was washed with distilled water, 9 times the weight of purified water was mixed with 155 g of dried Schisandra chinensis pulp, extracted for 3 hours at 60° C. and under normal pressure, and filtered under reduced pressure to obtain 39.868 g of Schisandra chinensis extract.

Example 2: Preparation of hot water extract of crushed Schisandra chinensis

Schisandra chinensis was washed with distilled water and dried Schisandra chinensis pulp was pulverized, 9 times the weight of purified water was mixed with 155 g of crushed Schisandra chinensis pulp, extracted for 8 hours at 60°C and under normal pressure conditions, filtered the extract, and filtered under reduced pressure to extract Schisandra chinensis 48.055 g was obtained.

Table 1 is a table comparing the extraction efficiency (%) with respect to the Schisandra chinensis extract extracted for 1 hour and 3 hours in Example 1 and the Schisandra chinensis extract extracted for 3 hours and 8 hours in Example 2.

Referring to Table 1 below, it can be seen that as the extraction time increases, the extraction efficiency increases.

Extraction conditions Example 1 Example 2 Extraction time(h) 1h 3h 3h 8h Extraction efficiency (%) 20.81 26.94 21.85 25.61

< Experimental example >

Experimental Example 1: Measurement of antimicrobial activity by concentration by disc diffusion assay

The antimicrobial activity of the Schisandra chinensis extracts prepared from Examples 1 and 2 was measured by the Disc diffusion assay method for the test strain. The concentration of the extract was 200 mg/ml, 100 mg/ml, 50 mg/ml, 20 mg/ml, and 10 mg/ml, respectively, diluted in sterilized distilled water and sterilized at 121.0° C. for 15 minutes at high temperature and pressure, and used on a plate medium. Each cultured strain was wiped with a sterilized cotton swab, transferred to a liquid medium, suspended, and smeared 50 ul to prepare. After attaching a paper disc (diamerter 8mm, Roshi kaisha.Ltd., Tokyo, Japan) to the plate medium coated with the strain, the sample is placed on a paper disc so that it is 4 mg, 2 mg, 1 mg, 0.4 mg and 0.2 mg per disc. After slowly absorbing P.acnes, the clear zone (mm) generated after 7 2 hours of anaerobic incubation in a 37°C incubator was measured, and S.epidermidis and S.aureus were incubated in a 37°C incubator for 48 hours and then around the disc. Antimicrobial activity was compared by measuring the inhibitory ring (clear zone, mm) generated in. Sterile distilled water was used as a control.

2 is an image of a result of measuring the antimicrobial activity of Schisandra chinensis extracts prepared from Examples 1 and 2 of the present invention for each concentration by a disc diffusion assay, and Table 1 below shows Schisandra chinensis prepared from Examples 1 and 2 of the present invention. The results of measuring the antibacterial activity of the hot water extract are shown numerically.

Referring to Figure 2 and Table 1, it can be seen that both the pulverized and finely pulverized Schisandra chinensis extracts have antibacterial effects against S.aureus and S.epidermidi strains, whereas there is no antibacterial effect against P.acnes strains.

Strain Extraction conditions Transparent ring diameter size (mm) Extract concentration (mg/disc) 4.0 2.0 1.0 0.4 0.2 S.aureus Example 1 12.16 Hae-hwan Hae-hwan - - Example 2 11.33 Hae-hwan - - - S.epidermidi Example 1 12.66 Hae-hwan Example 2 10.66 - - - - P. acnes Example 1 - - - - - Example 2 - - - - -

Therefore, according to the present invention, it is possible to provide an extract of Schisandra chinensis, and by using it, it can be applied as an antibacterial composition that can be safely used without toxicity and side effects.

Hereinafter, a formulation example of a composition containing the Schisandra chinensis extract of the present invention will be described, but the present invention is not intended to limit it, but is intended to be described in detail.

Formulation Example 1. Preparation of powder

500 mg of Schisandra chinensis extract of Example 1 or 2

100 mg lactose

10 mg of talc

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2. Preparation of tablet

300 mg of Schisandra chinensis extract of Example 1 or 2

100 mg corn starch

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet preparation method.

Formulation Example 3. Preparation of Capsule

200 mg of Schisandra chinensis extract of Example 1 or 2

3 mg of crystalline cellulose

14.8 mg lactose

Magnesium stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare a capsule.

Formulation Example 4. Preparation of injection

600 mg of Schisandra chinensis extract of Example 1 or 2

Mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO _4, 12H ₂ O 26 mg

It is prepared with the above ingredients per ampoule according to a conventional injection preparation method.

Formulation Example 5. Preparation of liquid formulation

4 g of Schisandra chinensis extract of Example 1 or 2

10 g of isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to the usual preparation method of liquid formulations, add and dissolve each component in purified water, add an appropriate amount of lemon flavor, mix the above ingredients, add purified water, add purified water, adjust the total to 100 g, and fill in a brown bottle. It is sterilized to prepare a solution.

Formulation Example 6. Preparation of granules

1,000 mg of Schisandra chinensis extract of Example 1 or 2

Vitamin mixture right amount

Vitamin A acetate 70 ㎍

1.0 mg of vitamin E

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

Vitamin B12 0.2 ㎍

Vitamin C 10 mg

Biotin 10 ㎍

1.7 mg of nicotinic acid amide

Folic acid 50 ㎍

0.5 mg of calcium pantothenate

Suitable amount of inorganic mixture

1.75 mg ferrous sulfate

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dicalcium phosphate 55 mg

90 mg of potassium citrate

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules, but it is possible to arbitrarily modify the mixing ratio. After mixing the above ingredients according to a conventional granule preparation method, granules And can be used to prepare a health functional food composition according to a conventional method.

Formulation Example 7. Preparation of functional beverage

1,000 mg of Schisandra chinensis extract of Example 1 or 2

1,000 mg citric acid

100 g oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 mL with purified water

After mixing the above ingredients according to the normal health drink manufacturing method, stirring and heating the mixture at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and stored in a refrigerator. It is used to prepare the functional beverage composition of the invention.

The composition ratio is composed of ingredients suitable for a relatively preferred beverage in a preferred embodiment, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences, such as the demand class, the country of demand, and the purpose of use.

Claims (11)

It contains Schisandra chinensis extract as an active ingredient,
A composition for antibacterial, characterized in that it has antibacterial activity against Staphylococcus epidermidis. The method of claim 1,
The Schisandra chinensis extract is an antibacterial composition, characterized in that the extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The method of claim 1,
The Schisandra chinensis extract is an antibacterial composition, characterized in that the hot water extract. delete delete It contains Schisandra chinensis extract as an active ingredient,
A pharmaceutical composition for antibacterial, characterized in that it has antibacterial activity against Staphylococcus epidermidis. It contains Schisandra chinensis extract as an active ingredient,
A cosmetic composition for antibacterial, characterized in that it has antibacterial activity against Staphylococcus epidermidis. The method of claim 7,
Antibacterial cosmetic composition, characterized in that in the form of a solution, powder, emulsion, lotion, spray, ointment, aerosol, cream or foam. It contains Schisandra chinensis extract as an active ingredient,
A food additive for antibacterial, characterized in that it has antibacterial activity against Staphylococcus epidermidis. It contains Schisandra chinensis extract as an active ingredient,
A feed additive for antibacterial, characterized in that it exhibits antibacterial activity against Staphylococcus epidermidis. It contains Schisandra chinensis extract as an active ingredient,
A hygiene product for antibacterial, characterized in that it exhibits antibacterial activity against Staphylococcus epidermidis.

Images