KR102124574B1 - Method for producing natural complex composition having prevention of pests, offensive odor removal of barn, and sterilization effect - Google Patents

Abstract

The present invention relates to a natural composite composition having a prevention effect of pests and diseases, an offensive odor removal effect of pigpen and farm, and a sterilization effect, which shows a combination of sterilization and odor reduction effect by containing only natural substances such as nano-processed soapberry fruit extract, and to a manufacturing method thereof. The present invention comprises a natural medicinal fermentation manufactured by distilling after fermenting the soapberry fruit extract, a grapefruit extract, nano-copper particles, nano-silver particles, perilla frutescens, cinnamon, mint, Pulsatilla, and cypress powder with an EM active liquid.

Description

Method for producing natural complex composition having prevention of pests, offensive odor removal of barn, and sterilization effect}

The present invention relates to a natural composite composition having a pesticidal effect, odor removal and sterilization effect of barn and fish farming, and a method for manufacturing the same, and more specifically, consists of only natural substances such as fruitless fruit extract containing a saponin component and sterilizing action and odor The present invention relates to a method for preparing a natural composite composition having a pesticidal effect, a sterilization effect of a livestock farm and a farm, and a sterilizing effect.

Recently, as the social problems caused by bacterial contamination and odors in crop fields, livestock farms and fish farms have become serious, various chemical agents have been developed to solve them.

However, most of the drugs for sterilization and odor removal used in livestock farms and farms, etc., are mostly chemical drugs and contain antibiotics, so if they are continuously used in large quantities, they will have a secondary adverse effect on livestock or farmed fish and potentially human. Stability problems with food are also caused.

Therefore, there is a need to develop a drug that is harmless and eco-friendly using natural materials and has excellent effects such as sterilization and odor removal.

Registered Patent No. 10-1671576 "Antibacterial composition for removing livestock odor" (2016.10.26.)

Therefore, the present invention was devised to solve the above-mentioned problems.

The object of the present invention, consisting of only natural materials, such as fruit-free fruit extract containing a saponin component, prevents pests, which exerts a bactericidal action and a odor reduction effect in combination, and a method for preparing a natural complex composition having a sterilizing effect and removing odors in stalls and farms In providing.

In order to achieve the above object, according to the present invention "prevention of pests, natural complex composition having a sterilizing effect and the stench removal of livestock and fish farms," the fruitless extract; Grapefruit extract; Nanocopper particles; It is characterized by containing nanosilver particles.

As a preferred embodiment of the composition according to the present invention, the composition may further contain a natural medicinal fermentation product prepared by distilling the cotyledon, cinnamon, mint, baekduong, and cypress powders with an EM active liquid.

In addition, according to the present invention, "prevention of pests, natural complex compositions with sterilizing effect and removal of odors from farms and farms," the step of extracting extracts of fruit-free fruit by adding fruit-free fruit and water to a stirrer and stirring (S1) Wow; Preparing (S2) a nanocopper dispersion by mixing and stirring a 1% nanocopper aqueous solution to the extract of the patient-free fruit extracted in step (S1); A step (S3) of preparing a nanosilver dispersion by mixing and stirring a 1% nanosilver aqueous solution in the nanocopper dispersion prepared in step (S2); It characterized in that it comprises a step (S4) of preparing a grapefruit dispersion by mixing and stirring the grapefruit extract in the nano-silver dispersion prepared in the step (S3).

As an embodiment of the production method according to the present invention, the step (S1) is to add the fruit-free fruit and water to the stirrer in a weight ratio of 1: 10 and stir to extract the fruit-free fruit extract containing 0.5 to 20% by weight of saponin And, the step (S2) is a 1% nanocopper aqueous solution is mixed with the non-patient fruit extract in a volume ratio of 1: 2 to 4, and the step (S3) is a 1% nanosilver aqueous solution relative to the total 100% by weight of the nanocopper dispersion. 1 to 10% by weight, the step (S4) may be mixed with grapefruit extract in 10% by weight relative to 100% by weight of the total nano-silver dispersion.

In addition, as a preferred embodiment, the step (S4) after the natural grapefruit dispersion mixed in the grapefruit dispersion prepared in the step (S4) and stirred and may further include the step of filtering (S5), the natural Herbal fermentation is mixed with 10% by weight relative to the total 100% by weight of the grapefruit dispersion, the natural herbal fermentation is 1: 1: 1: 1: 1 of the lobular, cinnamon, mint, baekduong, and cypress powder It is prepared by distilling it after distilling it for 10 days by adding EM active liquid at 5 vol% with respect to the amount of alcohol contained in alcohol (ethyl alcohol) at a weight ratio, and the EM active liquid is 30% by weight of lactic acid bacteria, 30% by weight of yeast bacteria, and 30% by weight of lead bacteria , 5% by weight of acetic acid bacteria, and 5% by weight of photosynthetic bacteria, and then incubated in the medium for 7 days at 30 ~ 40 ℃ and then incubated for 14 days at 20 ~ 30 ℃ without supply of the medium to prepare the EM stock solution , Dandelion, Machihyeon, thrips, Eoseongcho, Sambaekcho, rosemary, rose petals, goji berries, and burdock are mixed and hot water extracted to add 20 상기 of the above EM stock solution per 1 liter of extracted extract and 20 g of sugar is added to ferment for 30 days. Can be.

As described above, the present invention, even without the use of a drug containing antibiotics, it is possible to achieve a complex pest prevention, sterilization and odor removal effect. In particular, it is made of only natural materials, so there is no fear of environmental pollution, and there is no adverse effect even if livestock, farmed fish, or seafood are consumed.

Therefore, ultimately, the productivity and quality of livestock or fishery products may be improved, and food stability problems caused by excessive use of antibiotics may be solved.

1 is a block diagram schematically showing a method for preparing a natural composite composition having a pesticidal effect, sterilization effect and removal of offensive odors and farms according to the present invention.

Hereinafter, a preferred embodiment of the present invention will be described in more detail with reference to the accompanying drawings. However, it should be understood that the present invention can be implemented in many different forms and is not limited to the described embodiments.

The present invention relates to a liquid composition in which saponin, nanonized copper, nanonized silver, grapefruit extract, and natural medicinal fermentation products extracted from a patient-free fruit are mixed and dispersed, and a method for manufacturing the same.

First, each component included in the natural composite composition according to the present invention will be described.

Soap Nut (Soap Nut or Soap Berry) is a fruit that is held in the sapindus mukorossi and contains a lot of saponin in the bark of the acupuncture fruit, which is a natural surfactant and is widely used as a detergent, cosmetic raw material, feed additive, etc. In agriculture, it has resistance to nematodes and parasites, and as a farming material, it is a glycoside in which sugar and non-sugar parts are combined chemically. It has the property of producing a fine foam of persistence, like hydrophilicity and hydrophobicity when shaking aqueous solutions. At the same time it is a natural surfactant.

Since the over-spraying of pesticides destroys the surface material of plants and adversely affects the production of crops, saponin farming is applied in the agricultural field to improve the quality, increase the yield and increase the resistance of pests and pests while reducing the use of these pesticides. Saponins also affect the sugar content, gloss, and size of the fruit.

In addition, saponins keep the physiological function of the plant constant, and at the same time, protect the surface of the plant from drying, and prevent cell nutrients from being lost as rain. In addition, it protects plants from the invasion of pests and the East Sea and Frost Sea.

In the present invention, the non-patient fruit extract refers to a saponin extract containing saponin extracted from the non-patient fruit.

Nano silver has a broad and strong antibacterial and antiseptic effect against pathogenic viruses, bacteria, fungi, etc. In general, silver ions (Ag+) and reduced metal silver (Ag) are used, but in the nano silver aqueous solution used in the present invention An ion type having a size of 0.5 to 10 nm can be adopted.

As a method of manufacturing the nanosilver particles, a known technique may be adopted. For example, a water-soluble silver salt such as silver nitrate (AgNO ₃ ) is dissolved in water, and after adding a polymer dispersant or surfactant, hydrazine (H ₄ N ₂ ), can be obtained by introducing a reducing agent such as sodium borohydride (NaBH ₄ ), the nanosilver aqueous solution used in the present invention can be prepared by known techniques.

Nanocopper is a nanoparticle-sized copper, and copper (Cu) is an intrinsic property of metal and has antibacterial, sterilizing, and deodorizing properties. The nanocopper aqueous solution used in the present invention can likewise be prepared by a known technique, and an ion type having a size of 0.5 to 10 nm may be adopted.

Grapefruit contains a large amount of natural ascorbic acid, naringin, and tocopherol, so it has excellent antibacterial and antioxidant properties for various foods, and inhibits the production of toxic and decay products. It is a non-toxic, non-metallic, non-corrosive, colorless, odorless natural organic compound that does not cause environmental pollution, and has strong sterilization, virus, and fungus action against decaying and pathogenic microorganisms such as bacteria and virus fungi.

The natural medicinal fermentation product according to the present invention is produced by distilling the cotyledon, cinnamon, peppermint, baekduong, and cypress powders with an EM active solution, and having sterilization, deodorization, and deodorant efficacy while containing useful microorganisms.

The cotyledon, cinnamon, peppermint, Baekduong, and Cypress are all natural medicines having the effect of sterilization, insecticidal, deodorization, etc., and the EM active liquid is a medicinal material extract (extract) to be described later, lactic acid bacteria, yeast bacteria, lead bean bacteria, acetic acid bacteria, photosynthesis It is produced by fermenting bacteria with the cultured EM stock solution.

The natural composite composition according to the present invention, as described above, saponin-free fruit extract, naringin contains a large amount of grapefruit extract, nanometer-scale copper component, nanometer-scale silver component, sterilization, deodorant natural medicine The compound and effective microorganisms are combined to multiply the efficiency and efficacy of each component to achieve a complex synergistic effect of sterilization, removal of pests and odors, and improvement of the growth environment.

Hereinafter, the production method of the present invention using the composition component will be described in detail.

The method for preparing a natural complex composition having the effect of preventing and controlling odors and sterilization of barn and fish farms according to the present invention includes extracting fruit-free fruit extract (S1), preparing nanocopper dispersion (S2), preparing nanosilver dispersion (S3), Grapefruit dispersion preparation step (S4), and natural pharmaceutical fermentation product mixing and filtering step (S5).

The extracting of the fruitless fruit extract step (S1) is a step of extracting the fruitless fruit extract by adding the fruitless fruit and water to a known stirrer, followed by stirring, and extracting the fruitless fruit (preferably, a fruitless fruit peel) and water in a stirrer. It is a process of extracting the fruitless fruit extract containing 0.5 to 20% by weight of saponin relative to 100% by weight of the entire fruitless fruit extract by adding it at a weight ratio of 1:10 and stirring for a predetermined time.

Therefore, the fruitless fruit extract of the present invention means a saponin extract containing saponin, and as a more preferred embodiment, after the step (S1) obtains the saponin extract through a stirrer, the saponin extract is passed through a known filter. In the step (S1), a saponin extract, which is an impurity-removed saponin extract, is extracted in the step (S1).

The nanocopper dispersion preparation step (S2) is a step of preparing a nanocopper dispersion by mixing and agitating a 1% nanocopper aqueous solution with the patient-free fruit extract extracted in step (S1), wherein the patient-free fruit extract (ie, saponin extract) ) And a 1% nanocopper aqueous solution in a volume ratio of 1: 2 ~ 4, and using a known ball mill or attrition mill, etc. while stirring for a certain period of time, the average particle size of the nanocopper particles is 0.5 ~ This is a process of pulverizing and dispersing to a range of 10 nm.

The nano-silver dispersion manufacturing step (S3) is a step of preparing a nano-silver dispersion by mixing and stirring a 1% nano-silver aqueous solution in the nano-copper dispersion prepared in the step (S2), 1% to 100% by weight of the total nano-copper dispersion The average particle size of the nanosilver particles is 0.5 to 10 nm while the nanosilver aqueous solution is mixed at 1 to 10% by weight (or 1: 0.01 to 0.1 by volume) and similarly stirred for a certain time using a known ball mill or attrition mill (or stirrer). It is a process of pulverizing and dispersing to a range.

The grapefruit dispersion preparation step (S4) is a step of mixing the grapefruit extract in the nanosilver dispersion prepared in step (S3) and stirring to prepare a grapefruit dispersion, wherein the grapefruit extract is 10% by weight based on 100% by weight of the entire nanosilver dispersion. After mixing with (or 1: 0.1 by volume), it is a process to stir and disperse for a certain time in a known stirrer.

The grapefruit extract is a liquid grapefruit extract extracted by various known extraction methods. As an embodiment, the grapefruit extract is obtained by adding 28 liters of alkaline ionized water (pH 7.5 to 8) to 7 kg of grapefruit and extracting it by reflux, or by dispersing the grapefruit extract powder in water through a known nanonization process as a nano grapefruit dispersion aqueous solution. It may be obtained.

In addition, as a preferred embodiment, the natural medicine fermentation product mixture and filtration step (S5) is mixed with the natural medicine fermentation product in the grapefruit dispersion prepared in step (S4) after the step (S4), stirred and then filtered. As a step, it is a process of mixing the natural medicinal fermentation product at 10% by weight (or 1: 0.1 by volume) based on 100% by weight of the whole grapefruit dispersion, stirring for a period of time in a known stirrer and filtering with a known filter.

In the present invention, the natural medicinal fermentation product contains chlorophyll, cinnamon, peppermint, baekduong, and cypress powder in a 1: 1: 1: 1 weight ratio in alcohol (ethyl alcohol) and contains the EM active liquid in the amount of alcohol used. It is prepared by distillation after addition of 5 vol% for 10 days.

The alcohol refers to ethyl alcohol. In the present invention, alcohol is used to sufficiently extract the active ingredients of lobule, cinnamon, peppermint, baekduong, and cypress trees through alcohol fermentation.

The EM active solution is an EM stock solution in which useful microorganisms such as lactic acid bacteria, yeast bacteria, lead bacteria, acetic acid bacteria, and photosynthetic bacteria are cultured, and is prepared by fermenting natural medicines having sterilization, insecticidal, deodorizing, and deodorizing effects.

In a preferred embodiment, the EM active liquid is mixed with 30% by weight of lactic acid bacteria, 30% by weight of yeast, 30% by weight of lead bacteria, 5% by weight of acetic acid bacteria, and 5% by weight of photosynthetic bacteria, and then in the medium for 7 days at 30 to 40°C. After cultivation, by incubating for 14 days at 20 ~ 30 ℃ without supply of medium to prepare EM stock solution, and then, as a natural medicinal herb having antiseptic, insecticidal, deodorizing, and deodorizing properties, dandelion, marchi, dogwormwood, eoseongcho, baekcho, rosemary, It can be prepared by mixing rose petals, goji berries, and burdock and extracting 20 ml of the EM stock solution per 1 liter of extract extracted by hot water extraction and adding 20 g of sugar.

According to the method for preparing a natural composite composition according to the present invention, a saponin component extracted from a fruitless fruit through a stirring process, a copper and silver component granulated in nanometer units, and various natural medicine components having sterilization and deodorizing effects, A liquid composite composition in which useful microorganisms and the like are stably dispersed can be prepared.

Hereinafter, a preferred embodiment of the manufacturing method according to the present invention will be described, and the effect of the natural composite composition prepared by the embodiment will be confirmed through experiments.

Example

10 kg of fruitless peel and 100 kg of water are added to a known stirrer and stirred at a temperature of 20 to 25° C. while maintaining the stirrer at 70 rpm, but 10 hours so that 2% by weight of saponin is extracted with respect to the total weight of the extracted fruitless fruit extract Stir over. The impurity-free fruit extract (ie, saponin extract) containing 2% by weight of saponin is removed impurities through a known filter device.

Subsequently, 1 liter of the patient-free fruit extract (saponin extract) and 2 liter of 1% concentration of nanocopper aqueous solution were mixed and pulverized and dispersed such that the average particle diameter of the nanocopper particles was 0.5 to 10 nm while stirring at 150 rpm for 3 hours through a ball mill. Prepare a dispersion.

Next, the nanocopper dispersion is mixed with 10% by weight of a 1% concentration nanosilver aqueous solution with respect to 100% by weight of the nanocopper dispersion, and similarly, pulverized and dispersed such that the average particle diameter of the nanosilver particles is 0.5 to 10 nm while stirring at 150 rpm for 3 hours through a ball mill. By preparing a nano silver dispersion.

Subsequently, the grapefruit extract (grapefruit extract) is mixed with 10% by weight based on 100% by weight of the total nanosilver dispersion and dispersed while stirring for 1 hour in a stirrer to prepare a grapefruit dispersion.

Next, 30% by weight of lactic acid bacteria, 30% by weight of yeast, 30% by weight of lead bacteria, 5% by weight of acetic acid bacteria, and 5% by weight of photosynthetic bacteria, and then cultured in a medium for 7 days at 30 to 40°C to supply the medium Without incubation for 14 days at 20 ~ 30 ℃ to prepare the EM stock solution.

Then, 300 g of dandelion, 300 g of gusset, 300 g of thrush, 300 g of Eoseongcho, 300 g of Sambaekcho, 300 g of rosemary, 300 g of rose petals, 300 g of wolfberry, and 300 g of burdock are mixed in 4 l of water and extracted with hot water to extract 1 EM of the EM stock solution in 1 l Pour into and add 20 g of sugar to ferment for 30 days to prepare the EM active liquid.

Subsequently, 400 g of cotyledon, 400 g of cinnamon, 400 g of mint, 400 g of Baekduong, and 400 g of cypress powder were put into 3 l alcohol, and then 150 cc of the EM active liquid was put into alcohol, and the alcohol itself was fermented under 36 degrees Celsius for 10 weeks, and then distilled to make it natural. Medication fermentation product (fermentation solution) is prepared.

Thereafter, the natural herbal fermentation product is mixed with 10% by weight based on 100% by weight of the whole grapefruit dispersion, stirred for 1 hour in a stirrer, and then filtered with a filter to prepare a liquid natural composite composition according to the present invention.

<Odor reduction experiment>

It was tested whether the natural composite composition prepared according to the above example has an effect of reducing odor in a slurry containing odor collected from piglets.

The slurry was collected from pigs that do not receive a fixed amount of feed, antibiotics and probiotics. In addition, to minimize the parameters of the slurry, samples of designated farms were used, and common ingredients were analyzed during each test to determine the uniformity of the slurry properties. In order to evaluate the odorous substances generated from the slurry, a container was prepared by modifying the method described in Zhu et al (1996), and the total capacity was prepared and used at 1,000 ml. After adding the slurry to 50% of the container, the temperature of the constant-temperature water bath was adjusted to 25°C to cause odor.

The composition according to the embodiment of the present invention was added 10% of the input amount of the slurry. The reaction time with the slurry was 24 hours. The odor reduction efficacy evaluation measured the concentrations of ammonia, hydrogen sulfide and amines in the odor-related substances generated in the simple slurry odor reaction vessel. For the measurement of the odor gas, a gas meter for a detector tube (GV-100S) was used. The concentrations of ammonia, hydrogen sulfide, and amine were evaluated by the indicated values when a dedicated detection tube was inhaled for 10 seconds according to the instruction manual and maintained at a constant value.

The odor reduction rate was analyzed by comparing the composition according to the example with the non-treated group without any preparation, and the results are shown in Table 1 below.

ammonia Amines Hydrogen sulfide Concentration (ppm) Reduction rate (%) Concentration (ppm) Reduction rate (%) Concentration (ppm) Reduction rate (%) No treatment 21.20 - 64.00 - 612.00 - Example composition 9.60 54.71 28.20 55.93 276.15 54.87

As can be seen from Table 1, when a natural composite composition according to an embodiment of the present invention was added to a slurry containing odor-causing components such as ammonia, amines, and hydrogen sulfide (10% of the amount of slurry), ammonia measurement concentration Is reduced by about 54%, amine concentration by about 55%, and hydrogen sulfide concentration by about 54%.

Therefore, according to the experimental results, it was proved that the natural composite composition according to the present invention has an effect of removing or reducing malodor.

<Sterilization experiment>

Table 2 shows the results of requesting the sterilization experiment from the Korea Far Infrared Application Evaluation Research Institute for the natural composite composition according to the present invention prepared by Examples.

Test Items Sample classification Initial concentration Concentration after 24 hours Rate of bacteria reduction (%) Fever Blank 2.5 ×10 ⁵ CFU/ml 6.5 ×10 ⁵ CFU/ml - Example composition 0.57 ×10 ⁵ CFU/ml 77.2% Bungo Bacteria Blank 2.5 ×10 ⁵ CFU/ml 1.6 ×10 ⁶ CFU/ml - Example composition 0.51 ×10 ⁵ CFU/ml 79.6%

Test method: FKIA-FI-1003, Blank was measured without the composition of the present invention.

As shown in Table 2, compared to the state in which the composition according to the embodiment was not added (Blank), it showed a sterilizing effect of 77.2% in the case of blast fungus and 79.6% in the case of Lyophilus fungus. Therefore, according to the results of the experiment, it was confirmed that the natural composite composition according to the present invention has excellent bactericidal activity and thus can prevent pests caused by bacteria when applied to cultivated crops.

The preferred embodiments of the present invention have been described above, but the present invention can use various changes, modifications, and equivalents. It is clear that the present invention can be equally applied by appropriately modifying the above embodiments. Therefore, the above description is not intended to limit the scope of the present invention as defined by the following claims.

Meanwhile, in the detailed description of the present invention, specific embodiments have been described, but it will be apparent to those skilled in the art that various modifications are possible without departing from the scope of the present invention.

Claims (5)

delete delete A step (S1) of extracting a fruit-free fruit extract by adding the fruit-free fruit and water to the stirrer and stirring;
A step (S2) of preparing a nanocopper dispersion by mixing and stirring a 1% nanocopper aqueous solution in the extract of the patient-free fruit extracted in step S1;
A step (S3) of preparing a nanosilver dispersion by mixing and stirring a 1% nanosilver aqueous solution in the nanocopper dispersion prepared in the step (S2);
The step (S4) of preparing a grapefruit dispersion by mixing and stirring the grapefruit extract in the nano-silver dispersion prepared in the step (S3);
Characterized in that the prevention of pests and pests, the method of manufacturing a natural composite composition having a sterilizing effect and the removal of odors in barns and farms According to claim 3,
In the step (S1), the fruitless fruit and water are added to the stirrer in a weight ratio of 1: 10 and stirred to extract the fruitless fruit extract containing 0.5 to 20% by weight of saponin,
In the step (S2), the non-patient fruit extract and 1% nanocopper aqueous solution are mixed in a volume ratio of 1: 2 to 4,
In the step (S3), 1% nano silver aqueous solution is mixed with 1 to 10% by weight based on 100% by weight of the total nanocopper dispersion,
The step (S4) is nano-silver dispersion, 100% by weight of the grapefruit extract with respect to the total weight of 10% by weight, characterized in that pest control, odor removal and sterilization of farms and farms, natural composite composition manufacturing method. The method of claim 3 or 4,
After the step (S4), further comprising the step of mixing and stirring the natural medicinal fermentation product in the grapefruit dispersion prepared in the step (S4) and then filtering (S5),
The natural herbal fermentation product is mixed at 10% by weight relative to the total 100% by weight of the grapefruit dispersion,
The natural medicinal fermentation product contains the essence leaf, cinnamon, peppermint, baekduong, and cypress powders in ethyl alcohol at a weight ratio of 1:1:1:1:1, and contains the EM active liquid at 5Vol. After fermentation for 10 days by addition, distillation is performed,
The EM active liquid,
30% by weight of lactic acid bacteria, 30% by weight of yeast, 30% by weight of lead bacteria, 5% by weight of acetic acid, and 5% by weight of photosynthetic bacteria, and then incubated in the medium for 7 days at 30 to 40°C, without supply of medium. After incubating for 14 days at 30°C to prepare an EM stock solution, a mixture of dandelion, marchi string, thrush, eoseongcho, baekcho, rosemary, rose petals, goji berry, and burdock is extracted by hot water and the EM stock solution is extracted per 1 liter of the extracted extract. 20㏄ and 20g of sugar is added to ferment for 30 days, characterized in that it is produced by preventing pests and pests, removing odors from farms and farms, and producing a natural composite composition having a sterilizing effect.

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