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CN109105379A - A kind of preparation and application of chemical bactericide pesticide that preventing and treating blueberry fruit rot - Google Patents

A kind of preparation and application of chemical bactericide pesticide that preventing and treating blueberry fruit rot Download PDF

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Publication number
CN109105379A
CN109105379A CN201811111592.9A CN201811111592A CN109105379A CN 109105379 A CN109105379 A CN 109105379A CN 201811111592 A CN201811111592 A CN 201811111592A CN 109105379 A CN109105379 A CN 109105379A
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Prior art keywords
fruit rot
blueberry fruit
pesticide
preventing
chemical bactericide
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Inventor
孙家隆
梁晨
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Qingdao Agricultural University
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Qingdao Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

本发明涉及属于农药杀菌剂技术领域，具体涉及一种防治蓝莓果腐病的化学杀菌剂农药，按原料重量百分比计，其原料组成为：吡唑醚菌酯和DX110512（N‑（5‑三氟甲基‑3,4‑噻二唑‑2‑基）‑2‑氟苯甲酰胺）占总重量的50.0%，烯效唑占总重量的2.2%，助剂为5.0‑15.0%，填料载体为32.8‑42.8%。该杀菌组合物采用三种药剂作为主要成分，配以助剂和填料载体，达到了优势互补，相辅相成的作用。工艺简单，投入的原料全部转化为产品，收率高，无三废产生，有效提高了对蓝莓果腐病的防治效果。The invention relates to the technical field of pesticides and fungicides, in particular to a chemical fungicide pesticide for preventing and controlling blueberry fruit rot. According to the weight percentage of raw materials, the raw materials are composed of: pyraclostrobin and DX110512 ( N- (5-three Fluoromethyl-3,4-thiadiazole-2-yl)-2-fluorobenzamide) accounted for 50.0% of the total weight, uniconazole accounted for 2.2% of the total weight, auxiliary agent was 5.0-15.0%, filler Carrier is 32.8‑42.8%. The bactericidal composition adopts three kinds of medicaments as main components, and is matched with auxiliary agents and filler carriers, so as to achieve complementary advantages and complement each other. The process is simple, the input raw materials are all converted into products, the yield is high, no three wastes are generated, and the control effect on blueberry fruit rot is effectively improved.

Description

A kind of preparation and application of chemical bactericide pesticide that preventing and treating blueberry fruit rot
Technical field
The invention belongs to pesticide field, technical solution is related to a kind of prevention and treatment blueberry fruit rot, improves blueberry yield and change The preparation and application of the chemical pesticide of kind blueberry quality, and in particular to pyraclostrobin and a kind of ortho position substitution benzoyl compound And the application of the Recompounded pesticide of plant growth regulator uniconazole P prepared and its prevent and treat blueberry fruit rot.
Background technique
Blueberry English name Blueberry means blue berry, belongs to Ericaceae, cowberry platymiscium.Originating from North America, Perennial shrub small berries fruit tree.Because fruit is blue, therefore referred to as blueberry.One is low shrubs, and short-foot is wild, and particle is small, contain Abundant anthocyanidin；Second is artificial culture blueberry, can grow to 240cm high, and fruit is larger, and pulp is full, improves open country The eating mouth feel of raw blueberry, enhances absorption of the human body to anthocyanidin.The cowberry platymiscium of whole world distribution is up to more than 400.
Nutritional ingredient rich in Blueberry, having prevents cranial nerve aging, protection eyesight, heart tonifying, anticancer, soft Change blood vessel, the functions such as enhance the immune of the body, nutritional ingredient is high.Wherein, since blueberry is rich in anthocyanidin, there is activation retina Effect can strengthen eyesight, prevent eyeball fatigue and attract attention.Be also the five big healthy fruit recommended of the World Food Programme it One.And it is studied according to the U.S., Japan, European Section scholar, often edible blueberry product, can also significantly enhance eyesight, eliminate eye Eyeball fatigue.Clinical medicine report is also shown, and the anthocyanidin in blueberry can promote the visual purple in retina cell to regenerate, prevention Myopia promotes eyesight.
Blueberry is started late in China's cultivation, but cultivated area increases quickly, and relative disease problem is also with plantation Area expands and the time limit increases and becomes increasingly conspicuous, especially fruit rot evil, generates greatly harm to blueberry production, directly affects The yield and product quality of blueberry.It is found by further investigation, cause blueberry fruit rot mainly has Botrytis cinerea fruit rot (ash Grape sporeBotrytis cinerea) and rod method fruit rot (rod methodAlternaria alternata).Due to producing item The difference of part, both diseases occur simultaneously sometimes, individually occur sometimes.
Ortho position substitution benzoyl class compound occupies an important position in disinfectant use in agriculture, such as metalaxyl, Boscalid. This seminar conducts in-depth research analysis to ortho position substitution benzoyl class compound structure, has synthesized hundreds of ortho position substitutions Benzene/pyrazoles formyl class compound simultaneously determines its sterilization, bacteriostatic activity, and obtains multinomial country's invention authorization, such as CN103554052B、CN103524418B、CN103554098B、CN103242308B、CN103524407B、 CN103524419B, CN103535358B, CN103524417B, CN103554026B etc..Wherein 103535358 B of CN is disclosed Compound in, the compound that number is DX110512 shows extensive antibacterial, bactericidal activity, this seminar carries out it Further measurement, it is compounded with pyraclostrobin, it is found that the compound chemical pesticide has prevention and treatment effect well to blueberry fruit rot Fruit adds appropriate plant growth regulator uniconazole P in the compound preparation, and blueberry yield and quality significantly improves, gained ternary Compound preparation can be used as preventing and treating blueberry fruit rot raising blueberry yield and improve the dedicated agrochemicals fungicide of blueberry quality； Also, it is more environmentally friendly when producing as agrochemicals fungicide, the Serve " Agriculture conducive to industrialization.
DX110512
N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide
Pyraclostrobin (pyraclostrobin)
C19H18ClN3O4, 387.82,175013-18-0
Other title hectograms are quick, pyraclostrobin, Kai Run, F500, Headline, Insignia, Cabrio, Attitude.
Chemical name N- 2- 1-(4- chlorphenyl) and -1H- pyrazole-3-yl oxygen methyl ] phenyl } N- methoxy carbamate Methyl esters；Methyl N- 2- 1-(4-chlorophenyl) and -1H-pyrazol-3-yloxymethyl ] phenyl } N- methoxy carbamate。
Physicochemical property sterling appearance is white to oldlace tasteless crystalline solid.63.7 ~ 65.2 DEG C of fusing point, vapour pressure (20 ~ 25 DEG C): 2.6 × 10-8pa.N-Octanol/Water Partition: KowlogP=4.18(pH6.5).Henry constant 5.3 × 10-6Pam3 Mol-1(20 DEG C) 1.9mg/L(20 DEG C of solubility in water), solubility (20 DEG C, g/L) normal heptane 3.7 in other solvents, methanol 100, acetonitrile >=500, toluene, methylene chloride >=570, acetone, ethyl acetate >=650.N-octyl alcohol 24, DMF > 43.Raw medicine appearance For dark yellow, there is naphthalene taste liquid.Stability: hydrolysising half-life DT in water50> 30d, at pH5 ~ 7(25 DEG C) when stablize.Light in water Solve half-life period DT50< 2h.Field soil half-life period DT502~37d.Preparation storage at normal temperature: 2a stablizes at 20 DEG C.
Toxicity pyraclostrobin raw medicine rat acute LD50> 5000mg/kg, the percutaneous LD of rat acute50> 2000mg/kg.Acute inhalation LC50(4h) > 0.31mg/L.It is irritant to rabbit skin, to rabbit eye irritation；Without potential Mutagenicity, to rabbit, rat without potential teratogenesis, to mouse without potential carcinogenicity.NOEL data (2a, mg ∕ kgd): mouse great and mighty or powerful 3.4, female rat 4.6.Bobwhite acute oral LD50> 2000mg ∕ kg.Rainbow trout LC50(96h) > 0.006mg ∕ L.Water flea EC50 (48h)0.016 mg∕L.Algae EC50(96h) > 0.843 mg ∕ L.It is harmless to honeybee, LD50310 μ g ∕ of > is only (oral).Earthworm LC50(14d): 566mg ∕ kg(soil).
It is the wide spectrum Strobin class fungicide of BASF exploitation using pyraclostrobin, patent application day is in July, 1994 6 days.Pyraclostrobin is mainly used for cauline leaf and is sprayed, and can effectively prevent by Ascomycetes, Basidiomycetes, Fungi Imperfecti and oomycetes The fungus-caused crop disease of guiding principle.Not only toxicity is low for the compound, safe to non-target organism and equal to user and environment Safety is friendly.Conduction is permeated with protection, treatment, blade.
Uniconazole P (Uniconazole)
Molecular formula and molecular weight C15H18ClN3O 291.78
CAS accession number 83657-17-4
Other title High-Effective Thiazoles, special efficacy azoles, excellent health azoles, S-3307, Sumgaic, Prunit, Sumiseven
Chemical name(E)-(RS) -1-(4- chlorphenyl) -4,4- dimethyl -2- (1H- 1,2,4- triazol-1-yl) amyl- 1- alkene -3- Alcohol,(E)-(RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)-1- penten-3-ol
Physicochemical property sterling be colourless crystallization, 159 ~ 160 DEG C of fusing point, be slightly soluble in water, be soluble in acetone, ethyl acetate, chloroform and The common organic solvent such as dimethylformamide is stablized at 40 DEG C, does not decompose in multi-solvents and in acid, neutral, alkaline solution. But it is easily decomposed under the short light wave of 260 ~ 270nm.
Toxicity low toxicity.The acute LD of uniconazole P raw medicine50(mg/kg): rat oral 2020(hero), 1790(is female), percutaneous > 2000.Small white mouse acute oral LD50For 4000mg/kg(hero), 2850mg/kg(it is female)；Subacute toxicity, big white mouse are mixed into feed Maximum no-effect dose 2.30mg/kg(is male), 2.48mg/kg(it is female)；Without mutagenesis, teratogenesis, carcinogenesis；Have in short term to lagophthalmos Minor response, but have no stimulation of the skin, guinea pig skin (allergy) is feminine gender；Ichthyotoxin: carp TLM(48h) 6.36mg/L, flea (worm for fishing) TLM(3h) > 10mg/L.
Mechanism of action and feature uniconazole P are triazole type broad-spectrum plant growth regulators, are gibberellin synthetic inhibitors, and And there is certain bactericidal effect.There is strong inhibition growth to draft or woody unifacial leaf or dicotyledon.Main suppression The elongation of internodal cell processed.Uniconazole P can be absorbed via the root of plant, stem, leaf, neutron absorption by the root of plant, can be in vivo Conducted, when cauline leaf is spraying, can upward Uptake and translocation, but the effect that do not conduct downwards.The mechanism of action is identical as paclobutrazol, With control nutrient growth, inhibit cell elongation, shorten internode, dwarfed plant, promote lateral bud growth and flower_bud formation, increases anti- The effect of inverse property.Its activity is 6 ~ 10 times or more of paclobutrazol, 5 ~ 10 times generally lower than paclobutrazol using concentration, in the soil Residual quantity is only the 1/10 of paclobutrazol, therefore is influenced on succession crop small.
Suitable for crop can be used for field crop rice, wheat, increase tiller, controls plant height, improves power resistant to lodging；For fruit Tree and shrub reduce nutrient growth, control the tree-like of nutrient growth, improve fruit quality；Height is reduced for ornamental plant, is promoted Into flower_bud formation, increase is bloomed.
Summary of the invention
The purpose of the present invention: efficient one kind, low toxicity are provided, using safe prevention and treatment blueberry fruit rot, raising blueberry yield And improve the dedicated agrochemicals bactericide agricultural chemicals of blueberry quality, which has extremely blueberry fruit rot Significant preventive and therapeutic effect and it can significantly improve blueberry yield, improve blueberry quality.
A kind of prevention and treatment blueberry fruit rot, raising blueberry yield and the chemical sterilization for improving blueberry quality provided by the invention Agent pesticide, according to the weight percentage of raw materials, raw material composition are as follows: pyraclostrobin and DX110512(N(trifluoromethyl -3 5-, 4- thiadiazoles -2- base) -2- fluorobenzamide) weight account for the 50.0% of total weight, the weight of plant growth regulator uniconazole P Account for the 2.2% of total weight, auxiliary agent 5.0-15.0%, filling carrier 32.8-42.8%, wherein pyraclostrobin and DX110512 (N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) weight integer percent be 45-5:5-45, or It is expressed as 9-1:1-9.
In the present invention, pyraclostrobin is methoxy acrylic bactericide, and DX110512(N(5- trifluoromethyl- 3,4- thiadiazoles -2- bases) -2- fluorobenzamide) it is thiazole amide structure, bactericidal effect mechanism is different, two kinds of substances When combination, gained complex composition can act on target different loci simultaneously, play the role of mutual supplement with each other's advantages, can effectively improve pair The control efficiency of blueberry fruit rot, plant growth regulator uniconazole P can effectively improve blueberry yield, improve blueberry quality.
Heretofore described auxiliary agent be composite farm auxiliary agent, the sodium salt of naphthalene sulfonic acid formaldehyde polymer containing NNO(2-), K12(ten Sodium dialkyl sulfate), pull open powder (diisobutyl sodium naphthalene sulfonate), weight percent are as follows: NNO(2- naphthalene sulfonic acid formaldehyde polymer Sodium salt) 6.5%, K12(lauryl sodium sulfate) 1.5%, pull open powder (diisobutyl sodium naphthalene sulfonate) 2.0%.
Wherein NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt) play dispersing agent, K12(lauryl sodium sulfate) play profit Wet, osmosis, is conducive to crop absorbing composition effective component, and pull open powder (diisobutyl sodium naphthalene sulfonate) can significantly reduce water Surface tension, have excellent penetration and wetability, rewettability is also splendid, and have emulsification, diffusion and frothiness Energy.NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt), K12(lauryl sodium sulfate), pull open powder (diisobutyl sodium naphthalene sulfonate) three Person's science is used in compounding, and can effectively play dispersion, wetting, infiltration and suspension effect, it is effective to composition to facilitate crop The absorption of ingredient.
Filling carrier of the present invention is that diatomite can be widely used in the industrial production because of its good adsorption performance.
In the present invention, the pyraclostrobin and DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- Fluorobenzamide) preferred weight integer percent is 45-5:5-45(or is expressed as 9-1:1-9), optimum weight integer hundred Divide than being 25:25(or being expressed as 1:1).
By by pyraclostrobin and DX110512(in the present inventionN(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- Fluorobenzamide) by weight being 45:5,35:15,25:25,15:35,5:45 are compounded, with pyraclostrobin and DX110512 (N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) it compares, indoor toxicity test is carried out, as a result table Bright: compound preparation has significant synergistic effect, wherein with 25:25(or being expressed as 1:1 compared with single dose) effect is most prominent Out, joint Toxicological testing in specific steps and result detailed in Example Room 1.
It is a further object to provide the pyraclostrobin and DX110512(N(5- trifluoromethyl -3,4- thiophene Diazole -2- base) -2- fluorobenzamide) composition inhibit blueberry fruit rot opportunistic pathogen growth and kill blueberry fruit rot opportunistic pathogen in Purposes.
The beneficial effects of the present invention are: pyraclostrobin provided by the invention and DX110512(N(trifluoromethyl -3 5-, 4- thiadiazoles -2- base) -2- fluorobenzamide) agrochemicals bactericide agricultural chemicals can be used for preventing and treating blueberry fruit rot disease, have very Good inhibits the activity of blueberry fruit rot opportunistic pathogen growth, while can effectively improve blueberry yield, improve blueberry quality, in indigo plant Good effect can be obtained when concrete application in certain kind of berries production.
Pyraclostrobin and DX110512(provided by the inventionN(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorine Benzamide), plant growth regulator uniconazole P ternary built agrochemicals bactericide agricultural chemicals being for preventing and treating blueberry fruit rot, mention High blueberry yield, improvement blueberry quality are one of important features of the invention.
" three wastes " generated in preparation of compounds of the present invention are less, are easily handled, and sterilize as agrochemicals It is more environmentally friendly when agent pesticide producing.
Specific embodiment
The present invention passes through specific preparation and biological activity determination and crop field evaluation of pesticide effectiveness embodiment specific description pyrazoles ether Bacterium ester and DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide), plant uniconazole P ternary built The preparation and bioactivity of agrochemicals bactericide agricultural chemicals, the embodiment are only used for the specific description present invention rather than limitation originally Invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Joint toxicity measuring in embodiment Room 1
(1) prepared by PDA culture medium: by 200g peeling potatoes stripping and slicing, adding 1000mL distilled water, boils 30min.With gauze mistake Filter, adds distilled water to 1000mL.20g glucose and 17g agar is added, heating and melting dispenses, high pressure steam sterilization 25min, It is spare.
(2) culture of disease fungus: with a small amount of pathogen mycelia of transfer needle picking in PDA culture medium, being placed in 25 DEG C of perseverances It is cultivated 2-4 days in warm incubator, mycelia is stand-by after growing.
(3) measuring method: using mycelial growth rate method (size of colony diameter in certain time) measurement compound Bacteriostatic activity.5mg is accurately weighed in centrifuge tube for trying object, is first dissolved with proper amount of acetone, is added containing for calculation amount The aqueous solution of 1% polysorbas20 emulsifier, is diluted to the medical fluid of 1000mg/L.It takes 1mL medical fluid to be added in 9mL culture medium to shake up, prepare At the pastille culture medium of 100mg/L, the culture dish of diameter 90mm is uniformly poured into.Simultaneously with without for trying target compound, containing The culture dish of same amount of acetone and polysorbas20 emulsifier culture medium does blank control.Existed with the punch (diameter 5mm) of sterilizing Well-grown, pollution-free, the uniform colony edge of growing way is beatened to take bacteria cake, and it is (each aseptically to access pastille culture medium center Culture dish is inoculated with a bacteria cake), ware lid is covered, downward, every processing is repeated 3 times ware lid.Culture medium is placed in 21 DEG C of constant temperature incubations It is cultivated in case, measures each processing bacteria cake extension directly with crossing method when compareing that bacterium colony soon covers with culture dish in culture dish Diameter is averaged, and opposite bacteriostasis rate is calculated compared with blank control.
(4) calculating of bacteriostasis rate:
Bacterium colony increases diameter (mm)=bacterium colony measurement diameter (mm)-bacteria cake diameter (mm)
Relative inhibition (%)=[control bacterium colony increases bacterium colony in diameter (mm)-pastille culture medium and increases diameter (mm)]/right Increase diameter (mm) × 100 according to bacterium colony
(5) pyraclostrobin, DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) and pyrazoles Kresoxim-methyl and DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) composition setting 0.1 The culture medium progress antibiotic property survey of mg/L, 1.0 mg/L, 10.0mg/L, 50.0 mg/L, 100.0 mg/L, 5 concentration gradients It is fixed.Each it is repeated 3 times.And set acetone and PDA blank control.
Bacterium colony growth inhibition ratio is calculated according to the result measured, each medicament is calculated using log concentration-probability value method EC50, inhibitory activity of the evaluation reagent agent to target blueberry fruit rot pathogen growth.
(6) data processing method:
Each processing EC is calculated with SPSS50, medicament composition co-toxicity coefficient (CTC) is calculated with Sun Yunpei's Method:
Co-toxicity coefficient (CTC)=[mixture actual toxicity index (ATI) ÷ mixtures theoretical toxicity index (TTI)] × 100
Mixture actual toxicity index (ATI)=(EC of standard agent50The EC of ÷ mixture50) × 100
Content+B component of A in mixtures theoretical toxicity index (TTI)=component A toxicity index (TI) × mixture effective component The content of B in toxicity index (TI) × mixture effective component (in the present invention, A DX110512, B are pyraclostrobin)
Toxicity index (TI)=(EC of standard agent50The EC of ÷ reagent agent50) × 100
When co-toxicity coefficient is significantly greater than 100, show there is synergistic effect；Close to 100, show there is summation action；It is significantly less than 100, Show there is antagonism.
Test result:
Indoor joint toxicity measuring of the 1 pyraclostrobin DX110512 of table to blueberry grey mold fruit rot bacterium
72h is 108.28-235.41 to blueberry gray mold co-toxicity coefficient after medicine, the results showed that compound preparation is compared with single dose, tool There is significant synergistic effect, wherein with DX110512: effect is prominent when pyraclostrobin is 35:15 and 25:25,72h after medicine It is 232.71 and 235.41 to blueberry fruit rot co-toxicity coefficient.
Indoor joint toxicity measuring of the 2 pyraclostrobin DX110512 of table to blueberry rod method fruit rot bacterium
72h is 110.64-186.75 to blueberry Alternaria alternate co-toxicity coefficient after medicine, the results showed that compound preparation and single dose phase Than there is significant synergistic effect, wherein with DX110512: effect is most prominent when pyraclostrobin is 25:25,72h after medicine It is 186.75 to blueberry fruit rot co-toxicity coefficient.
According to indoor joint Toxicological testing, in conjunction with the requirement of blueberry yield and quality, determine preferred proportioning components and most preferably at Part proportion is as follows:
It is preferred that proportioning components are as follows:
Ingredient Wt percentage
Pyraclostrobin 45-5
DX110512 5-45
Uniconazole P 2.2
Auxiliary agent 5.0~15.0
Filling carrier 32.8-42.8
In view of the generation actual conditions of blueberry fruit rot and harm sequence, comprehensively considers the prevention and treatment of blueberry fruit rot and blueberry produces Amount and quality requirements, ternary built chemical agent best proportioning components when compounding are as follows:
Ingredient Wt percentage
Pyraclostrobin 25.0
DX110512 25.0
Uniconazole P 2.2
Auxiliary agent 10.0
Filling carrier 37.8
2 52.2% pyraclostrobin DX110512(of embodimentN(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzene first Amide) uniconazole P agrochemicals bactericide agricultural chemicals preparation
Component (purity/%) weight (kg)
Pyraclostrobin (98%) 255.2
DX110512(98%) 255.2
Uniconazole P (97%) 22.7
NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt) (96%) 67.8
Pull open powder (diisobutyl sodium naphthalene sulfonate) (98%) 20.5
K12(lauryl sodium sulfate) (98%) 15.4
Diatomite 363.2
Specific production operation: pyraclostrobin (98%) 255.2 kg, DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- Base) -2- fluorobenzamide) (98%) 255.2 kg, (97%) 22.7 kg, NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt of uniconazole P) (96%) 67.8 kg, (98%) 20.5 kg, K12(lauryl sodium sulfate of pull open powder (diisobutyl sodium naphthalene sulfonate)) (98%) 15.4 kg, 363.2 kg of diatomite on airslide disintegrating mill mixing machine be uniformly mixed, be delivered to stock chest, by charger into Enter the feed bin of airslide disintegrating mill, adjusts operating pressure and stream pressure, mixture are admitted to pulverizing chamber and are crushed.Pulverized Mixture enters clasfficiator by discharge port, and coarse powder returns to raw material tank by rotary valve and raw material crushes again together.It crushes and enters rotation Wind separator is collected in finished bin, and finished product and Ultramicro-powder mix in double helix cone blender, subsequently on packing machine Feed bin.Sample (250 g) analysis: composition size controlling is at 5 ~ 10 μm, pyraclostrobin >=25.0%, DX110512(N- (5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) >=25.0%, uniconazole P >=2.2%, NNO(2- naphthalene sulfonic acids first Aldehyde polymer sodium salt) >=6.5%, pull open powder (diisobutyl sodium naphthalene sulfonate) >=2.0%, K12(lauryl sodium sulfate) >=1.5%, Product is qualification；It packed, labelled according to national related pesticide regulations of rules, it is preceding after the assay was approved by launch, Paste quality certification label.
3 52.2% pyraclostrobin DX110512(of embodimentN(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorine Benzamide) test of uniconazole P chemical bactericide pesticide field control effect
Material to be tested: 52.2% pyraclostrobin DX110512(described in the embodiment of the present invention 2N(5- trifluoromethyl -3,4- thiophene Diazole -2- base) -2- fluorobenzamide) uniconazole P chemical bactericide pesticide；Comparison medicament is 50% pyraclostrobin wettable Pulvis (Qingdao Agricultural University's chemistry and pharmaceutical college's pesticide research and application topic group are prepared), 50% DX110512(N-(5- fluoroform Base -3,4- thiadiazoles -2- base) -2- fluorobenzamide) wettable powder (Qingdao Agricultural University's chemistry and pharmaceutical college's pesticide research It is prepared with application topic group).
Test method: application test is located at Qingdao City Qingdao Long Hui agricultural development Co., Ltd Han Jia and slips base, soil class Type is brown earth, is in subacidity, and fertility is higher.Blueberry be facility cultivation in 2015, kind be jewelry, it is close this ladder, Ao Ni'er, at random District's groups arrangement, is repeated 4 times, experimental plot area 40m2。
Spraying time: on 2 22nd, 2017, on March 8th, 2017, on March 22nd, 2017.
Application method: with 52.2% pyraclostrobin DX110512(N-(5- trifluoromethyl -3,4- thiophene two of the present invention Azoles -2- base) -2- fluorobenzamide) uniconazole P chemical bactericide pesticide, 50% pyraclostrobin wettable powder of comparison medicament With 50% DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) 600 times of wettable powder Liquid, 800 times of liquid, 1000 times of liquid difference spraying treatments infect the blueberry of fruit rot, every 666.7m2Spray 60 kg medical fluids.
Field control effect statistics: 7d, 14d, 21d after third time is administered, 28d i.e. 2017 on March 29,2017 On April 5, on April 12nd, 2017 respectively handle in experimental plot blueberry plant fruit rot a situation arises and investigated, counted Calculate the control efficiency of each processing disease index and medicament.Table 3 lists pyraclostrobin, DX110512 and its group after application 28d Object is closed to the preventive effect result of blueberry fruit rot, the results showed that 52.2% pyraclostrobin DX110512(N-(5- trifluoro of the invention Methyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) uniconazole P chemical bactericide pesticide experimental concentration to blueberries corruption The preventive effect of disease is significantly higher than other two kinds control single doses.
3 pyraclostrobin of table, DX110512 and 52.2% pyraclostrobin DX110512(N-(5- trifluoromethyl -3,4- Thiadiazoles -2- base) -2- fluorobenzamide) uniconazole P chemical bactericide pesticide field control effect
Data are found out from table 3,52.2% pyraclostrobin DX110512(N-(5- trifluoromethyl -3,4- thiophene of the present invention Diazole -2- base) -2- fluorobenzamide) uniconazole P chemical bactericide pesticide is apparently higher than phase to blueberry fruit rot control efficiency Pyraclostrobin, DX110512(with active constituent contentN(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzoyl Amine) single dose, and it is applicable to the prevention and treatment of the fruit rot of multi items blueberry.
4 52.2% pyraclostrobin DX110512(of embodimentN(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorine Benzamide) uniconazole P chemical bactericide pesticide Field information
Material to be tested: 52.2% pyraclostrobin DX110512(described in the embodiment of the present invention 2N(5- trifluoromethyl -3,4- thiophene Diazole -2- base) -2- fluorobenzamide) (Qingdao Agricultural University's chemistry is ground uniconazole P chemical bactericide pesticide with pharmaceutical college's pesticide Study carefully and prepared with using topic group).
Test method: application test is located at the Qingdao City Qingdao base Long Hui agricultural development Co., Ltd Lang Ya, soil types For brown earth, journey subacidity.Blueberry is facility cultivation in 2015, kind Lan Feng, likes to come, Bridges tower, test area is respectively 20×666.7m2、32×666.7m2、25×666.7m2。
Spraying time: on March 12nd, 2018, on March 26th, 2018, on April 9th, 2018.
Application method: with 52.2% pyraclostrobin DX110512(N-(5- trifluoromethyl -3,4- thiophene two of the present invention Azoles -2- base) -2- fluorobenzamide) 600 times of liquid spraying treatment trial zone blueberries of uniconazole P chemical bactericide pesticide, often 666.7m2Spray 60 kg medical fluids.
Field information effect statistics: by blueberry harvest, classification, weighing when fruit maturation, yield, evaluation product matter are calculated Amount.
4 output statistics result of table
Related disease statistical result: three kinds of trial zone blueberry does not have fruit rot evil.
Find out from concrete application data, 52.2% pyraclostrobin DX110512(N-(5- trifluoromethyl-of the present invention 3,4- thiadiazoles -2- bases) -2- fluorobenzamide) uniconazole P chemical bactericide pesticide Normalization rule, can significantly increase Blueberry yield, and applicability is preferable.Further quality track analysis is made to blueberry, the blueberry of discovery trial zone harvest is mellow and full to lure People, organic acid content are reduced, and soluble sugar, Vitamin C sugar and protein content are significantly raised.
Seminar passes through the practical application of embodiment 3 and embodiment 4 after further investigation in 3 years, to Lan Feng, happiness Six kinds of next, Bridges tower, jewelry, this close ladder, Ao Ni'er blueberries have carried out 52.2% pyraclostrobin DX110512(N-(5- tri- Methyl fluoride -3,4- thiadiazoles -2- base) -2- fluorobenzamide) verifying of uniconazole P chemical bactericide pesticide concrete application, test Data, which are shown, achieves good actual effect, illustrates 52.2% trifluoromethyl -3 pyraclostrobin DX110512(N-(5-, 4- thiadiazoles -2- base) -2- fluorobenzamide) uniconazole P chemical bactericide pesticide to blueberry fruit rot have well prevention and treatment Effect can be widely applied.
Related raw material source:
Pyraclostrobin: Nanjing Kun Yue Chemical Co., Ltd.；
DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide): Qingdao Agricultural University's chemistry with Pharmaceutical college's pesticide research and application topic group；
Uniconazole P: Anyang Quanfeng Agriculture Chemical Co., Ltd.；
K12(lauryl sodium sulfate): Run Jun Chemical Co., Ltd. of Suzhou City；
NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt): Taixing City perseverance source laboratory；
Pull open powder (diisobutyl sodium naphthalene sulfonate): Jinan Bao of the dye Chemical Co., Ltd.；
Diatomite: Hebei Xin Xu mineral products Co., Ltd；
50% DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) wettable powder: Qingdao Agriculture university's chemistry is with pharmaceutical college's pesticide research and using topic group；
50% pyraclostrobin wettable powder: Qingdao Agricultural University's chemistry is with pharmaceutical college's pesticide research and using topic group.
Remarks:
(1) 50% DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) wettable powder weight Measure percentage composition and preparation method: by DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) (98%) 5.11 kg, NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt) (96%) 0.313 kg, pull open powder (diisobutyl naphthalene sulfonic acids Sodium) (98%) 0.101 kg, K12(lauryl sodium sulfate) (98%) 0.0766 kg, 4.399 kg of diatomite is uniformly mixed, uses Use for laboratory pulverizer is crushed to 200 mesh or so fineness, is then crushed by the miniature airslide disintegrating mill in laboratory, and control fineness is 5 ~ 10 μm, content is then measured, is packaged spare after reaching this requirement of experiment.
(2) 50% pyraclostrobin wettable powder weight percents composition and preparation method: by pyraclostrobin (98%) 5.15 kg, NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salts) (96%) 0.364 kg, pull open powder (diisobutyl sodium naphthalene sulfonate) (98%) 0.141 kg, K12(lauryl sodium sulfate) (98%) 0.0686 kg, 4.276 kg of diatomite is uniformly mixed, with experiment Room is crushed to 200 mesh or so fineness with pulverizer, is then crushed by the miniature airslide disintegrating mill in laboratory, and control fineness is 5 ~ 10 μm, content is then measured, is packaged spare after reaching this requirement of experiment.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art 's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed Range.

Claims (9)

1. a kind of chemical bactericide pesticide for preventing and treating blueberry fruit rot, it is characterised in that: according to the weight percentage of raw materials, raw material Composition are as follows: pyraclostrobin and DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) it accounts for always The 50.0% of weight, uniconazole P account for the 2.2% of total weight, auxiliary agent 5.0-15.0%, filling carrier 32.8-42.8%.
2. a kind of chemical bactericide pesticide for preventing and treating blueberry fruit rot according to claim 1, it is characterised in that: pyrazoles ether Bacterium ester and DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) weight integer percent be 45-5:5-45 is expressed as 9-1:1-9.
3. a kind of chemical bactericide pesticide for preventing and treating blueberry fruit rot according to claim 2, it is characterised in that: pyrazoles ether Bacterium ester and DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide) weight integer percent be 25:25 is expressed as 1:1.
4. a kind of chemical bactericide pesticide for preventing and treating blueberry fruit rot according to claim 1, it is characterised in that: described Auxiliary agent is composite farm auxiliary agent, weight percent are as follows: NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt) 6.5%, K12(dodecyl Sodium sulphate) 1.5%, pull open powder (diisobutyl sodium naphthalene sulfonate) 2.0%.
5. a kind of chemical bactericide pesticide for preventing and treating blueberry fruit rot according to claim 1, it is characterised in that: described Filling carrier is diatomite.
6. a kind of preparation method of chemical bactericide pesticide for preventing and treating blueberry fruit rot described in claim 1, it is characterised in that: Specific steps are as follows: pyraclostrobin, DX110512(N(5- trifluoromethyl -3,4- thiadiazoles -2- base) -2- fluorobenzamide), Uniconazole P, NNO(2- naphthalene sulfonic acid formaldehyde polymer sodium salt), pull open powder (diisobutyl sodium naphthalene sulfonate), K12(dodecyl sulphate Sodium), diatomite be uniformly mixed and be crushed to 5 ~ 10 microns of partial size by airslide disintegrating mill, after the assay was approved by country's correlation pesticide Regulations of rules packaging is product.
7. a kind of chemical bactericide pesticide of prevention and treatment blueberry fruit rot described in claim 1 is inhibiting growth of pathogenic bacteria or is killing The application gone out in pathogen；Wherein disease caused by the pathogen does not include the disease of human body or animal body.
8. a kind of chemical bactericide pesticide of prevention and treatment blueberry fruit rot described in claim 1 inhibits growth of pathogenic bacteria in preparation Or the application in killing pathogen drug.
9. a kind of purposes of chemical bactericide pesticide for preventing and treating blueberry fruit rot according to claim 1, it is characterised in that: As the application in disinfectant use in agriculture.
CN201811111592.9A 2018-09-24 2018-09-24 A kind of preparation and application of chemical bactericide pesticide that preventing and treating blueberry fruit rot Pending CN109105379A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110376341A (en) * 2019-06-24 2019-10-25 广西大学 A kind of measuring method of graw mold of tomato protective agents photolysis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103535358A (en) * 2013-10-18 2014-01-29 孙家隆 Bactericide usage of group of ortho substituted benzoyl compounds

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103535358A (en) * 2013-10-18 2014-01-29 孙家隆 Bactericide usage of group of ortho substituted benzoyl compounds

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110376341A (en) * 2019-06-24 2019-10-25 广西大学 A kind of measuring method of graw mold of tomato protective agents photolysis

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