KR101996079B1 - Composition for preventing or treating hair loss comprising enterococcus faecalis - Google Patents

Abstract

The present invention relates to a composition for preventing or treating hair loss comprising Enterococcus faecalis as an active ingredient. Specifically, it has been confirmed that cell growth is promoted in human dermal papilla cells treated with Enterococcus faecalis EF-2001 dead cells, The culture of the Enterococcus faecalis of the present invention or its culture can be used as an active ingredient of a composition for preventing or treating hair loss, or for promoting hair growth,

Description

TECHNICAL FIELD The present invention relates to a composition for prevention or treatment of hair loss comprising Enterococcus faecalis as an active ingredient,

The present invention relates to a composition for preventing or treating hair loss comprising Enterococcus faecalis as an active ingredient and more particularly to a composition for prevention or treatment of hair loss comprising Enterococcus faecalis , A composition for treating hair growth, or a composition for promoting hair growth.

 In today 's modern society, the appearance lives in a time when the phrase "power beyond imagination" reaches too much. Regardless of age and sex, we can see how much we consider our appearance in our society. The external criterion occupies a large part of the criteria for judging people, and the hair style has a big influence on the face which occupies the largest weight. Therefore, mental stress caused by hair loss can lead to loss of self-confidence, lack of interpersonal attraction, lack of sociality, and in severe cases, loss of enthusiasm for life and deterioration of quality of life.

In the past, hair loss has been regarded as the exclusive property of men, but as the number of women who worry about their hair getting lost as they go to modern society increases, it can be seen that men and women are both young and old. Hair loss also occurs in young people in their 20s and 30s, and the age at which hair loss occurs gradually tends to decrease. As described above, the number of patients with hair loss is rapidly increasing, so that the worries and anxieties are deepening. Therefore, studies on the causes, countermeasures and therapeutic agents of hair loss have attracted attention.

In addition, there is an increase in the number of pets holding a pet. In some cases, the pets are molted according to the season, but hair loss or hair loss due to endocrine diseases may occur, and the cosmetic problem is different. Which may adversely affect the performance of the apparatus.

The hair repeats cycle in the order of anagen, catagen, and telogen. The duration of the growth period is 2 ~ 6 years, which is 85 ~ 90% of the total hair follicle, and the retrograde period has 2 weeks duration and it occupies 2 ~ 3% of the total hair follicle. Finally, the dormant period has a duration of 3 to 4 months and accounts for 10 to 15% of the total hair follicles. Such an overall cycle is repeated. This cycle is gradually shortened, and the hair which is about 30 ~ 100 times a day is no longer falling out or growing is called alopecia.

 Alopecia is largely classified into three categories: congenital alopecia with no hair, acquired alopecia with progressive aging, and alopecia due to pathological reasons. Acquired alopecia are the most common alopecia areata, genetic alopecia and alopecia due to the action of androgens on the hair (androgenic alopecia), neurogenic alopecia, non-alopecia, alopecia, juvenile alopecia and senile alopecia Alopecia due to cause includes postpartum alopecia, alopecia after fever, and drug poisoning alopecia. It has been found that various causes of hair loss are not due to one factor such as genetic factors, male sex hormones, stress, aging, eating habits, and environmental factors.

Minoxidil and Propecia are the most effective drugs to prevent or treat hair loss at present. Minoxidil was originally intended to lower blood pressure by dilating capillaries with blood pressure lowering agents. However, it has been known that adverse effects improve blood circulation in the hair follicles by thickening the hair follicles and affect the hair growth. However, it also affects the fur on the whole body. In addition to the hair, the hairs on the whole body grow thicker and thicker, and because it plays a role of lowering the blood pressure, people with hypotension should not use it. You should refrain from using it. The ingredient name of Propecia is finasteride, developed by Merck Pharmaceuticals of America. This ingredient has been developed to treat benign prostatic hyperplasia, but it has been shown that it can promote hair growth in the course of research, and has been used as a remedy for hair loss. The cause of hair loss is that testosterone is irreversibly metabolized by 5α-reductase to dihydro testosterone (DHT), and DHT binds to androgen receptor (AR) Biologic effect. In human hair follicles, AR is mainly distributed in the dermal papilla cells, and DHT binds to AR in dermal papilla cells to promote the secretion of hair growth inhibitory factors such as TGF-β. Such inhibitory factors lead to death of mosquito cells. As a result, hair growth period of hair follicles becomes shorter and miniaturization of hair follicles causes hair loss. Propecia thus inhibits hair loss by inhibiting the 5α-reductase that causes hair loss and decreasing the concentration of DHT. However, Propecia is a male hormone, so women are prohibited from taking it, side effects are loss of sexual desire or sexual dysfunction is known to have been recently reported in patients with depression and mood deformity was added as a note. Therefore, there is a need to develop a hair loss therapeutic agent having less side effects than minoxidil and propecia, and having excellent effects.

On the other hand, Enterococcus faecalis EF-2001 ( Enterococcus faecalis EF-2001) were identified through intestinal flora screening of a 2-year-old girl. Studies on the physiological activity of Enterococcus faecalis EF-2001 have shown that in mice suffering from Dextran sulfate sodium-induced colitis, mice receiving Enterococcus faecalis EF-2001 have a DSS mitigation effect , And the proliferation of transplanted sarcoma-180 cells (Sarcoma-180) was reduced and NK cells were activated. Biochemically, it was also reported that mice consuming Enterococcus faecalis EF-2001 had a lower ratio of total cholesterol and triglyceride even after eating a high fat diet. Regarding the suppression of the harmful microorganisms and the effect of the dressing, the effect of the antibiotic-administered mice on the proliferation of the beneficial bacteria and the inhibition of the harmful bacteria is disclosed as compared with the intestinal control. However, the effect of preventing the hair growth or hair loss of Enterococcus faecalis is not known at all.

As a result, the present inventors have made efforts to develop a hair loss therapeutic agent having few side effects and excellent effects. As a result, it has been confirmed that cell growth is promoted in human dermal papilla cells treated with Enterococcus faecalis EF-2001 cells as a lactic acid bacterium, The present invention has been accomplished on the basis of the finding that Enterococcus faecalis, a culture solution thereof, or a bacterium thereof can be used as an active ingredient of a composition for preventing or treating hair loss.

Korean Patent No. 10-0729446 Korean Patent No. 10-1434717

Choi, Yeon - hee (2000) A Study on Causes and Coping Strategies of Hair Loss. Han JH, Kwon OS, Chung JH, Cho KH, Eun HC, Kim KH (2004) Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci 34 (2): 91-98. Kyung-Suk Kim (2015) Analysis of cell protective effects and mechanisms of Ellagic acid against UVA-induced damages in hDPCs. Kor J Aesthet Cosmetol 13 (6): 833-840.

It is an object of the present invention to provide an enterococcus faecalisEnterococcus faecalis), A composition for preventing or treating hair loss or a composition for promoting hair growth, which comprises the culture liquid or its dead cells as an active ingredient.

In order to accomplish the above object, the present invention relates to an enterococcus The present invention also provides a pharmaceutical composition for prevention or treatment of hair loss, comprising a culture solution thereof or a bacterium cell thereof as an active ingredient.

The present invention also provides a cosmetic composition for preventing hair loss or promoting hair growth comprising Enterococcus faecalis, a culture solution thereof or a bacterium cell thereof as an active ingredient.

Further, the present invention provides a health functional food for preventing hair loss or promoting hair growth comprising Enterococcus faecalis, a culture solution thereof or a bacterium cell thereof as an active ingredient.

Further, the present invention provides a livestock feed additive for preventing hair loss or hair loss, a cosmetic composition for a livestock, or a skin external preparation for livestock comprising the culture of Enterococcus faecalis, a culture thereof or a bacterium thereof as an active ingredient.

The present invention relates to the use of Enterococcus faecalis EF-2001 cells have been found to promote the growth of human dermal papilla cells, which play a pivotal role in regulating the development and growth of hair follicles. Therefore, the enterococcus faecalis of the present invention, The cells can be used as an active ingredient of a composition for preventing or treating hair loss, or a composition for promoting hair growth.

Figure 1 is a graph showing the effect of Minoxidil on human dermal papilla cell or Enterococcus faecalis ) EF-2001 cells were treated and the degree of cell growth was confirmed.

Hereinafter, the present invention will be described in more detail.

The present invention relates to the use of Enterococcus The present invention also provides a pharmaceutical composition for prevention or treatment of hair loss, comprising a culture solution thereof or a bacterium cell thereof as an active ingredient.

In the present invention, the Enterococcus faecalis is preferably Enterococcus faecalis EF-2001, but is not limited thereto.

The Enterococcus faecalis EF-2001 is a lactic acid bacterium developed by BRM Research Institute, Inc. of Nihon Berumu Co., Ltd. It is preferable to promote the growth of human dermal papilla cells.

In the present invention, the Enterococcus faecalis, the culture thereof or the microbial cells thereof may be commercially available or may be any of those produced by a known microbial cell production method, and it is not toxic and harmless to the human body.

Also, the culture solution means a culture product of cultured Enterococcus faecalis in a culture medium, a concentrated culture solution, a dried culture product, a cultured filtrate solution, a concentrated culture filtrate solution or a culture filtrate solution. , And may be a culture obtained by removing the strain after culturing.

The dead cells may be prepared by heat-treating the corresponding live cells or treating them with formalin or other bactericides, and the dead cells may be substantially dead. The bacterial cells are cultured by a conventional method, and the obtained strains are washed, centrifugally dehydrated, washed and dehydrated repeatedly, if necessary, suspended in distilled water, physiological saline or the like, A suspension of the cell suspension obtained by heating at 80 to 115 캜 for 30 minutes to 3 seconds or a dried product thereof or a suspension of the cell suspension obtained by irradiating the cell suspension with a gamma ray or a neutron beam. The drying method of the dead cell suspension is not particularly limited as long as it is a known drying method, and examples thereof include spray drying and freeze drying. In some cases, the enzyme treatment, the surfactant treatment, the crushing and crushing treatment can be carried out before or after the sterilization treatment by heating or before or after the drying treatment, and those obtained by such treatment are also included in the dead cells of the present invention do. In addition, the dead cells can be produced by the following method, but are not limited thereto:

1) seed culture of Enterococcus faecalis followed by culture at pH 5.0-8.0 at 20-40 ° C;

2) heat-treating the cultured Enterococcus faecalis cultivated in step 1) at a temperature of 70 to 130 ° C for 5 to 30 minutes, followed by drying and pulverizing.

In the present invention, the hair loss may be, for example, circular hair loss, male hair loss, feminine hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss or postpartum hair loss.

In a specific example of the present invention, the present inventors produced Enterococcus faecalis EF-2001 dead cells. In order to examine the hair growth effect of the dead cells, human dermal papilla cells were treated with the dead cells, It is possible to use the culture of Enterococcus faecalis of the present invention or the culture thereof or the microbial cells thereof as an active ingredient of a composition for preventing or treating hair loss or a composition for promoting hair growth.

The composition of the present invention may contain the strain as an active ingredient in an amount of 10 ⁶ to 10 ¹³ cfu / g, based on the total weight of the composition, or may include culture or dead cells having an equivalent number of live cells. The effective amount of the enterococcus faecalis of the present invention which is the main component of the present invention, the culture solution thereof or the microbial cells thereof may be selected from one or two or more kinds of pharmaceutically acceptable conventional carriers or one or more additives, ≪ / RTI >

The carrier may be selected from one or more selected from among diluents, lubricants, binders, disintegrants, sweeteners, stabilizers and preservatives. Examples of the additives include one or more of flavorings, vitamins and antioxidants Can be used.

In the present invention, the carrier and the additive may be any pharmaceutically acceptable ones. Specific examples of the diluent include lactose monohydrate, trehalose, cornstarch, soybean oil, Microcrystalline cellulose or D-mannitol may be used as the lubricant and magnesium stearate or talc may be used as the lubricant. The binder may be polyvinyl pyrolidone (PVP) or And hydroxypropylcellulose (HPC). The disintegrant is preferably selected from carboxymethylcellulose calcium (Ca-CMC), sodium starch glycollate, polacrylin potassium or cross-linked polyvinylpyrrolidone And the sweetening agent is selected from white sugar, fructose, sorbitol or aspartame. Examples of the stabilizer include sodium carboxymethylcellulose sodium (Na-CMC), beta-cyclodextrin (beta -cyclodextrin) white bee's wax or xanthan gum and the preservative may be selected from the group consisting of methyl p-hydroxy benzoate, methylparaben, propyl p-hydroxybenzoate, propylparaben, or potassium sorbate potassium sorbate, but is not limited thereto.

The pharmaceutical compositions of the present invention may be administered to a patient in a single dose and may be administered by a fractionated treatment protocol in which multiple doses are administered over a prolonged period of time. The term "pharmaceutically effective amount" as used herein refers to an amount that exhibits a reaction higher than that of the negative control, and preferably refers to an amount sufficient to prevent or treat an inflammatory disease. In addition, the pharmaceutically effective amount may be appropriately changed according to various factors such as the disease and its severity, the patient's age, body weight, health condition, sex, administration route and treatment period.

The composition of the present invention can be variously formulated according to the route of administration by a method known in the art together with the pharmaceutically acceptable carrier. The term "pharmaceutically acceptable" as used herein means a non-toxic composition which is physiologically acceptable and which, when administered to humans, does not inhibit the action of the active ingredient and does not normally cause an allergic reaction such as gastrointestinal disorder, dizziness, . The composition of the present invention can be variously formulated according to the route of administration by a method known in the art together with the pharmaceutically acceptable carrier. Routes of administration include, but are not limited to, oral or parenteral administration.

The present invention also provides a cosmetic composition for preventing hair loss or promoting hair growth comprising Enterococcus faecalis, a culture solution thereof or a bacterium cell thereof as an active ingredient.

In the present invention, the term " hair loss prevention "or" hair growth promoting "is used interchangeably and has the same meaning as another term wool or hair growth promotion used in the art.

In the present invention, the Enterococcus faecalis is preferably Enterococcus faecalis EF-2001, but is not limited thereto.

Furthermore, it is preferable that the Enterococcus faecalis EF-2001 promotes the growth of human dermal papilla cells.

In the present invention, the hair loss may be, for example, circular hair loss, male hair loss, feminine hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss or postpartum hair loss.

The formulations of the cosmetic composition according to the present invention may be any formulations which can contain the enterococcus faecalis, the culture liquid thereof or the dead cells thereof as an active ingredient. Examples of the formulations for preventing hair loss or promoting hair growth include hair tonic, hair lotion, hair But is not limited to, various forms such as cream, hair spray, hair mousse, hair gel, hair conditioner, hair shampoo, hair rinse, hair book, hair treatment, eyebrow hair extender, eyelash hair extender or eyelash nutritional agent.

The cosmetic composition includes components commonly used in cosmetic compositions and includes, for example, customary adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. In addition, the cosmetic composition according to the present invention may be used in combination with a conventional hair care composition or hair restorer, which has been conventionally used, in addition to the effect of the present invention (hair growth function), in addition to the enterococcus faecalis, have.

As the carrier, it is possible to use purified water, monohydric alcohols (ethanol or propyl alcohol), polyhydric alcohols (glycerol, 1,3-butylene glycol or propylene glycol), higher fatty acids (palmitic acid or linolenic acid) But are not limited to, camellia oil, jojoba oil, olive oil, squalane, sunflower oil, macadamia peanut oil, avocado oil, soybean water-added lecithin or fatty acid glyceride). If necessary, a surfactant, a bactericide, an antioxidant, an ultraviolet absorber, an anti-inflammatory agent and a refreshing agent may be added.

The surfactant is selected from the group consisting of polyoxyethylene, hardened castor oil, polyoxyethylene, oleyl ether, polyoxyethylene monooleate, polyoxyethylene, glyceryl monostearate, monostearic acid sorbitan, monooleic acid polyoxyethylene, sorbitan, Polyglycerol fatty acid ester, sucrose fatty acid ester, monolauric acid hexaglycerin, polyoxyethylene reduced lanolin, POE, glyceryl pyroglutamic acid, isostearic acid, diester, N-acetylglutamine and isostearyl ester.

The fungicide may optionally be selected from the group consisting of: hynochitol, trichloro acid, chlorohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid and zinc pyrithione have.

As the antioxidant, any of butylhydroxyanisole, gallic acid, propyl gallate, and erythorbic acid may be used.

The ultraviolet absorber may be at least one selected from the group consisting of benzophenones such as dihydroxybenzophenone and the like, melanin, ethyl paraaminobenzoate, 2-ethylhexyl paradimethylbenzoate, synonym, 2-ethylhexyl phamethoxycinnamate, 2- Hydroxy-5-methylphenyl) benzotriazole, urocanic acid, and metal oxide fine particles.

As the anti-inflammatory agent, dipotassium glycyrrhizinate or allantoin can be used, and as a refreshing agent, pepper tincture or 1-menthol can be used.

The present invention also provides an external skin preparation for preventing hair loss or promoting hair growth comprising Enterococcus faecalis, a culture solution thereof or a bacterium cell thereof as an active ingredient.

In the present invention, the Enterococcus faecalis is preferably Enterococcus faecalis EF-2001, but is not limited thereto.

Furthermore, it is preferable that the Enterococcus faecalis EF-2001 promotes the growth of human dermal papilla cells.

In the present invention, the hair loss may be, for example, circular hair loss, male hair loss, feminine hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss or postpartum hair loss.

The external preparation for skin according to the present invention can be applied to the skin. In addition, in order to prevent hair loss or accelerate hair growth, it is important to select a suitable external preparation type. Therefore, the enterococcus faecalis, the culture solution thereof or the bacterium cells thereof can be produced with an ointment agent so that it can be effectively applied to the affected part. The ointment preparation is prepared by blending an Enterococcus faecalis culture, a culture thereof or a bacterium thereof with an inorganic substance and then coating it with a liposoluble base. The inorganic material is preferably a material having excellent antimicrobial activity, anti-inflammatory effect, epidermal regeneration effect, etc. Specific examples thereof include zinc oxide, zinc carbonate, iron oxide and the like. In addition, it is preferable to further use a ceramic carrier capable of safely impregnating a pharmaceutical composition for treating skin diseases of the present invention which is a water-soluble substance. As the ceramic carrier, zeolite, talc, gypsum, mortar and mixtures thereof are preferably used. Such a ceramic carrier is excellent in the impregnation property of the water-soluble component, so that the water-soluble component can be smoothly supplied to the skin.

Further, the present invention provides a health functional food for preventing hair loss or promoting hair growth comprising Enterococcus faecalis, a culture solution thereof or a bacterium cell thereof as an active ingredient.

In the present invention, the Enterococcus faecalis is preferably Enterococcus faecalis EF-2001, but is not limited thereto.

Furthermore, it is preferable that the Enterococcus faecalis EF-2001 promotes the growth of human dermal papilla cells.

In the present invention, the hair loss may be, for example, circular hair loss, male hair loss, feminine hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss or postpartum hair loss.

The health functional foods of the present invention include, for example, folk remedies for the purpose of improving bowel function such as tea, jelly, juice, extract, and beverage. The health functional food of the present invention processed in various forms as described above is easy to take and can be stored for a long time without adverse effects on the human body.

When the enterococcus faecalis, the culture thereof or the bacterium cells thereof of the present invention is used as a food additive, the enterococcus faecalis, the culture thereof or the bacterium thereof may be directly added or used together with other food or food ingredients, And the like. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). In general, when the food or beverage is produced, it is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on 100 parts by weight of the Enterococcus faecalis, the culture liquid thereof, However, in the case of long-term consumption intended for health and hygiene purposes or health control purposes, the amount may be less than the above range, and there is no problem in terms of safety. Therefore, the active ingredient may be used in an amount of more than the above range.

There is no particular limitation on the kind of the food. Examples of foods to which the Enterococcus faecalis, the culture thereof or the bacterium thereof can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice confection, ice cream, milk , Dairy products including milk substitute, cream, butter, buttermilk, yogurt, yoghurt, cheese, various soups, drinks, tea, drinks, alcoholic beverages and vitamin complexes. do.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The above-mentioned natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau Martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally 0.001 to 1.0 g, preferably about 0.01 to 1.0 g per 100 mL of the Enterococcus faecalis of the present invention, the culture liquid thereof or the bacterium thereof.

The enterococcus faecalis of the present invention, its culture or its microbial cells may be used as a nutrient, a vitamin, an electrolyte, a flavoring agent, a colorant, a pectic acid or a salt thereof, an alginic acid or its salt, an organic acid, a protective colloid thickener, , Stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the strain of the present invention, its culture or its dead cells may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but it is common that the strain or culture of the present invention is selected in the range of 0.001 to 0.1 part by weight per 100 parts by weight.

The present invention also provides an animal hair growth preventing or hair growth stimulating livestock feed additive comprising Enterococcus faecalis, a culture thereof or a bacterium cell thereof as an active ingredient.

In the present invention, the Enterococcus faecalis is preferably Enterococcus faecalis EF-2001, but is not limited thereto.

Furthermore, it is preferable that the Enterococcus faecalis EF-2001 promotes the growth of human dermal papilla cells.

In the present invention, the hair loss may be, for example, circular hair loss, male hair loss, feminine hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss or postpartum hair loss.

In the present invention, the livestock may be, but is not limited to, a dog, a cat, a rabbit, a sheep, a goat, a pig, a horse, a donkey, a cattle, a cow, a duck, a chicken or a goose.

In addition, the animal feed additive includes a binder, an emulsifier, a preservative, and the like added to prevent quality deterioration. In order to increase the efficiency of the animal feed additive, amino acids, vitamins, enzymes, flavors, Buffers, extractants, and oligosaccharides. In addition, feed mixtures and the like may be further included, but the present invention is not limited thereto.

In addition, the present invention provides a cosmetic composition for preventing hair loss or promoting hair growth comprising at least one selected from the group consisting of Enterococcus faecalis, a culture thereof, and dead cells thereof as an active ingredient.

In the present invention, the Enterococcus faecalis is preferably Enterococcus faecalis EF-2001, but is not limited thereto.

Furthermore, it is preferable that the Enterococcus faecalis EF-2001 promotes the growth of human dermal papilla cells.

In the present invention, the hair loss may be, for example, circular hair loss, male hair loss, feminine hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss or postpartum hair loss.

In the present invention, the cosmetic composition is useful as a cosmetic composition for pet hair tonic, pet hair hair lotion, pet hair cream, pet hair spray, pet hair mousse, pet hair gel, pet hair conditioner, Hair shampoos for pets, Hair conditioner for pets, Hairdresser for pets, Hair treatment for pets, Eyebrows for pets, Hair lollipop for pets or Hair lollipop for pets , But is not limited thereto.

In addition, the present invention provides an external skin preparation for animal hair for hair loss prevention or hair growth promoting, which comprises, as an active ingredient, at least one selected from the group consisting of Enterococcus faecalis, a culture solution thereof and a dead cell thereof.

In the present invention, the Enterococcus faecalis is preferably Enterococcus faecalis EF-2001, but is not limited thereto.

Furthermore, it is preferable that the Enterococcus faecalis EF-2001 promotes the growth of human dermal papilla cells.

In the present invention, the hair loss may be, for example, circular hair loss, male hair loss, feminine hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss or postpartum hair loss.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the present invention is not limited to the following Examples and Experimental Examples.

< Example  1> Enterococcus Lacalis ( Enterococcus faecalis ) EF -2001 Dead cells  Produce

Enterococcus faecalis ) EF-2001 dead cells were prepared as follows.

Specifically, Enterococcus faecalis EF-2001 live cells (Nihon BRM Co. Ltd, Japan) were aerobically or anaerobically cultured in a medium used for culturing general lactic acid bacteria, followed by pre-culture and maintained at pH 5.0 to 8.0 and 20 to 40 ° C And cultured for 1 to 3 days to reach the number of cells of 7.5 × 10 ¹² cfu / g or more based on dry weight (DW). Then, it was heat-treated at 70 to 130 ° C for 5 to 30 minutes and then quenched. After heat treatment, the cells were separated and recovered by a continuous centrifuge, followed by lyophilization and pulverization.

< Example  2> human Dermal papilla cells (human dermal papilla cell) culture

Human dermal papilla cells (hDPCs) are known to play a pivotal role in regulating the development and growth of hair follicles. In order to examine the hair growth effect of Enterococcus faecalis EF-2001 cells, human dermal papilla cells were cultured as follows.

Specifically, human dermal papilla cells (hDPCs) were divided into 100 mm-dishes and cultured in DMEM high glucose medium containing 10% FBS and 1 × antibiotic / antimycotics at 37 ° C and 5% CO ₂ . Cells were maintained by subculture once every 2-3 days.

< Experimental Example  1> Enterococcus Lacalis EF -2001 On dead cells  Confirmation of hair growth effect by

In order to examine the hair growth effect of Enterococcus faecalis EF-2001 cells, the human dermal papilla cells cultured in Example 2 were treated with the Enterococcus faecalis EF-2001 cells prepared in Example 1 And cell growth was confirmed.

Specifically, human dermal papilla cells cultured in Example 2 were divided into 96 well-plates at 1.5 × 10 ⁴ cells / well, followed by 1 × antibiotic / antimycotics And cultured in DMEM high glucose medium at 37 ° C and 5% CO _{2 for} 24 hours. In addition, the cells were diluted with DMEM medium to a concentration of 50, 100, 150, 250 and 500 쨉 g / ml of the Enterococcus faecalis EF-2001 cells prepared in Example 1. Then, cells cultured for 24 hours were treated with the cells of Enterococcus faecalis EF-2001 diluted by the respective concentrations and cultured for 72 hours. After 72 hours, 20 μl of 5 mg / ml MTT solution was added. The cells were incubated at 37 ° C and 5% CO _{2 for} 4 hours. After removing the supernatant, the cells were dried for 5 hours under dark conditions. Then, 200 μl of DMSO was added and dissolved for 20 minutes. Then, absorbance was measured at 595 nm to measure the degree of cell growth. The MTT solution exhibits color change from yellow to purple by mitochondrial reductase of living cells. Therefore, it is possible to measure the degree of cell growth by measuring the absorbance at a specific wavelength.

As a positive control, human dermal papilla cells treated with 1 μM minoxidil (Sigma) instead of Enterococcus faecalis EF-2001 were used.

As a result, as shown in Fig. 1, it was confirmed that the cell growth of human dermal papilla cells treated with Enterococcus faecalis EF-2001 was promoted up to 12.4% than the positive control human dermal papilla cells treated with minoxidil. Particularly, when the Enterococcus faecalis EF-2001 cells were treated at a concentration of 150 μg / ml, the cell growth promotion reaction of human dermal papilla cells was found to be the best.

Therefore, the enterococcus faecalis EF-2001 microbial cells of the present invention show growth-promoting effect on flow cells of all humans, and thus can be used as an effective ingredient of compositions for preventing, treating, and promoting hair loss.

Claims (13)

A pharmaceutical composition for preventing or treating hair loss, comprising as an active ingredient at least one selected from the group consisting of Enterococcus faecalis EF-2001, a culture thereof and a bacterium thereof.
delete The pharmaceutical composition for preventing or treating hair loss according to claim 1, wherein the Enterococcus faecalis EF-2001 promotes growth of human dermal papilla cells.
[Claim 5] The pharmaceutical composition for preventing or treating hair loss according to claim 1, wherein the microbial cells are heat-treated with Enterococcus faecalis EF-2001 at a temperature of 70 to 130 DEG C for 5 to 30 minutes.
The hair loss preventive method according to claim 1, wherein the hair loss is selected from the group consisting of circular hair loss, male hair loss, female hair loss, neurogenic hair loss, non-rigid hair loss, seborrheic hair loss, mature hair loss, senile hair loss, Or a pharmaceutically acceptable salt thereof.
Enterococcus faecalis EF-2001, a culture thereof, and a bacterium thereof, as an active ingredient.
The cosmetic composition according to claim 6, wherein the cosmetic composition is at least one selected from the group consisting of hair tonic, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair conditioner, hair shampoo, hair rinse, hair book, hair treatment, Wherein the composition is selected from the group consisting of an eyelash nourishment agent and an eyelash nourishment agent.
Wherein the composition contains at least one selected from the group consisting of Enterococcus faecalis EF-2001, a culture thereof, and dead cells thereof as an active ingredient.
Wherein the active ingredient is at least one selected from the group consisting of Enterococcus faecalis EF-2001, a culture thereof and a bacterium thereof.
Wherein the composition comprises at least one selected from the group consisting of Enterococcus faecalis EF-2001, a culture thereof, and dead cells thereof as an active ingredient.
Enterococcus faecalis EF-2001, a culture thereof, and a bacterium thereof, as an active ingredient.
12. The composition of claim 11, wherein the cosmetic composition is selected from the group consisting of pet hair tonic, pet hair lotion, pet hair cream, pet hair spray, pet hair mousse, pet hair gel, Conditioner, Hair shampoo for pets, Hair conditioner for pets, Hair conditioner for pets, Hair conditioner for pets, Hair conditioner for pets, Hair conditioner for pets, Hair conditioner for pets, Hair conditioner for pets, Hair conditioner for pets Wherein the composition is a formulation.
Wherein the composition contains at least one selected from the group consisting of Enterococcus faecalis EF-2001, a culture thereof, and dead cells thereof as an active ingredient.

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