KR101434717B1 - Composition for preventing hair loss or promoting hair growth comprising extracts of Panax ginseng and Hijikia fusiforme - Google Patents

Abstract

The present invention provides a composition for preventing hair loss or promoting hair growth comprising an extract of Panax ginseng and Hijikia fusiforme as an active ingredient. The composition of the present invention can alleviate the progress of hair loss, promote wool / hair growth and growth of hair, and is effective in preventing hair loss or promoting hair growth.

Description

[0001] The present invention relates to a composition for preventing hair loss or promoting hair growth, which contains ginseng and top extract,

The present invention relates to a composition for preventing hair loss or promoting hair growth.

The causes of hair loss have been discussed in male or female organs such as hair follicles, hair follicle blood flow, bacterial growth or scalp irregularity due to excessive sebum secretion, genetic factors, and aging Korean Patent Application No. 10-2006-0067229, 2006.07.19, page 2, [0002] paragraph). However, the definite cause of hair loss has not yet been clarified. Recently, people who are suffering from alopecia are increasing and the age group is lowering.

There are no clear effects on the treatment of alopecia, and there are some prescriptions for taking hair loss, such as diagnosis of neonatal volume in Donguibogam, massage of sesame seeds on hair loss site, And the method of stimulating specific blood circulation. However, it is known that there are many individual differences in the effect, and there is not yet reported a new drug substance which can be generally applied.

The human body has more than 1.3 million hairs and more than 100,000 hairs. These hairs go through three cycles of anagen, catagen, and telogen, and each hair is run in an independent cycle. Hair loss refers to a state in which hair or hair is less or no more than normal due to any cause (Korean Patent Application No. 10-2005-0015941, Feb. 25, 2005, paragraph [0002]), The distinction between hair loss (telogen effluvium) and abnormal hair loss phenomenon (anagen effluvium) is distinguished, the latter is generally referred to as hair loss. Alopecia Areata, Androgenetic Alopecia, Telogen Effluvium, Traumatic, Trichotillomania, Pressure, and Alopecia are the most common forms of hair loss (or hair loss disease) (Alopecia syphiltiac), Alopecia seborrhecia, symptomatic, scarring, congenital alopecia, and the like.

As a conventional method for treating alopecia, there is a preparation based on female hormone in relation to hormonal theory, but its use has been discontinued due to reports of skin inflammation and adverse effects caused by administration of hormone. Minoxidil of the US-based company and trichosaccharide of Crinos Co. are used as hair growth promoters, but there is a problem of lack of clear effects and side effects (Korean Patent Application 10-2008-0086248, September 2, 2008, page 2, [0005] paragraph). In addition, finasteride, developed by Merck, is known to inhibit the activity of 5-α-reductase, an enzyme that acts on the male hormone testosterone metabolism in hair follicles, but can cause depression This also has the problem that long-term treatment is necessary.

In addition, there are Crescina and Rogaine, but only when the hair follicle is alive on the normal scalp before the hair loss disease is fully progressed. Thus, various conventional hair growth promoting methods have generally been attempted to promote hair growth with the aim of promoting blood circulation and nourishment of hair follicles, but the toxicity and side effects are also severe and the effect thereof is insufficient at present (Korean Patent Application No. 10-2006-0021965, March 2003, pp. 4-5, [0015] to [0016] paragraph).

Ginseng (Panax ginseng CA Meyer: Ginseng radix) is known as one of the most important medicines used mainly in Chinese medicine, and it is listed in many Chinese books including China. It is used for restoring fatigue, (Korean Ginseng) (1996), which has been used as a sole or prescription constituent herbal medicine for the control of functions of the ginseng, The effects of ginseng on saponin have been studied and the pharmacological effects of central nervous system, various stress, antidiabetic and cardiovascular disorders have been clarified (Benishin CG et al., Effects of Ginsenoside Rb1 on Central Cholinergic (1991), Kang SY et al., Dietary ginsenosides improve endotheliumdependent relaxation in the thoracic aorta of hypercholesterolemic rabbit (1995), Elma ZT et al., Effect of ginsenoside Rg 1 on insulin- ). However, in addition to saponin, polyacetylene, a non-saponin component of ginseng, has been shown to inhibit cancer cell proliferation, and studies on the physiological activities of active ingredients other than saponin have been made (Hwang WI et al., Comparative Study on the Cytotoxic Activities of Red Ginseng of Korea and China, 10: 27-35,1996).

Based on this herbal medicine efficacy, many experimental studies and clinical efficacy studies conducted during the period have been presented various efficacies of ginseng. In addition, through the study of active ingredients, more than 30 kinds of glycosides such as ginsenosides and non-saponin-based physiologically active substances, which are glycoside compounds, are widely used as the main active ingredients of ginseng as polyacetylenes, phenolic compounds, acidic polysaccharides, peptides, alkaloids, and amino acid derivatives. Other ingredients of ginseng include volatile oil, sugar, starch, pectins, minerals, etc. (Namkijiru, the latest Korean ginseng (ingredient and efficacy), 1996 ). In recent years, there has been a growing interest in ginseng as a tonic or health food, as well as alternative medicines, in Western Europe as well as in East Asia (Smith et al., 1999).

Seaweeds have been known to contain higher amounts of vitamins and dietary fiber than land-based organisms, and contain many essential minerals necessary in the body, such as magnesium, iron, iodine, calcium, and zinc (Lee JW, Sung NJ, The Content Carbohydrates in seaweeds prevent cholesterol deposition in the blood vessels and facilitate intestinal motility, accelerate the release of heavy metals, and are effective in the improvement of hyperlipidemia. J Food Sci Nutr 1980; 9 (1): 51-7. (Food Science Nutr. 1998; 27 (4): 718-23.), The physiological and physiological effects of food poisoning Attention has been focused on the identification of active substances and the development of functional foods.

Hijikia fusiforme is a perennial seaweed that lives in the lower part of the intertidal zone about 30cm above the low water level and exists only in the metamorphic age. In Korea, it grows from the southern part of Jumunjin to the west coast of Jangsan, and it is widely distributed in South coast and Jeju area. It belongs to the classification system, Heterokontophyta, Phaeophyceae, Fucales, Sargassaceae and Hizikia. Laminaran, a neutral polysaccharide known to have functional properties such as blood coagulation and immunity enhancement as well as a good source of dietary fiber, contains a large amount of fucoidin, a sulfuric acid polysaccharide, and other antioxidant , Antimicrobial activity, etc., and high content of minerals such as calcium is known to have excellent efficacy in prevention and treatment of various adult diseases and chronic diseases. In addition, it has been confirmed that not only cholesterol deposition in blood vessels is prevented, but also intestinal motility is smoothly performed, so that it is effective not only for discharging heavy metals, but also has an anticancer effect.

Korean Patent Registration No. 10-0788608, December 26, 2007, page 2, paragraph [0002] Korean Patent Publication No. 10-2006-0095062, 2006.08.30, 2, [0002] paragraph Korean Patent Publication No. 10-2010-0027360, 2010.03.11, page 2, paragraph [0005] Korean Patent Publication No. 10-2007-0092052, Sep. 12, 2007, 4-5, [0015] to [0016] Korean Patent Publication No. 10-2008-007762, 2008.8.26, Summary

Nam Ki-ri, the latest Korean ginseng (ingredient and efficacy), 1996 Benishin CG et al., Effects of Ginsenoside Rb1 on Central Cholinergic Metabolism (1991) Elma ZT et al., Effect of ginsenoside Rg 1 on insulin binding in mouse liver and brain membrane (1991) Kang SY et al., Dietary ginsenosides improve endothelium-dependent relaxation in the thoracic aorta of hypercholesterolemic rabbit (1995) Hwang WI et al., Comparative Study on the Cytotoxic Activities of Red Ginseng of Korea and China, 10: 27-35, 1996 Smith M et al., Counseling cancer patients about herbal medicine, 1999 Lee JW, Sung NJ. The Content of Minerals in Algae. J Food Sci Nutr., 9 (1): 51-7, 1980. Kim HS, Kim GJ. Effects of the Feeding Hijikia fusiforme (Harvey) Okamura on Lipid Composition of Serum in Dietary Hyperlipidemic Rats. J Food Sci Nutr., 27 (4): 718-23, 1998. Hideaki Matsuda et al., Promotion of Hair Growth by Ginseng Radix on Cultured Mouse Vibrissal Hair Follicles, Phytother. Res. 17, 797-800, 2003

A problem to be solved by the present invention is to provide a composition effective in preventing hair loss or promoting hair growth.

In order to achieve the above object, the present inventors have searched a variety of active substances that can prevent hair loss or promoted hair growth in natural products having excellent safety. As a result, it was surprisingly found that a mixed extract of ginseng and tortoise Cells and keratinocytes, and that the present invention has been accomplished.

Accordingly, the present invention provides a composition for preventing hair loss or promoting hair growth comprising an extract of Panax ginseng and Hijikia fusiforme as an active ingredient.

The ginseng (Panax ginseng CA Meyer. Ginseng radix) is a perennial herbaceous plant belonging to Panax ginseng (Araliaceae). It has been used for a long time as a medicinal herb and has been used for various pharmacological treatments Has been regarded as the best health food in the world. The chemical composition of ginseng consists of saponin, essential oil, polyacetylene, phenol, alkaloid, polysaccharide, amino acid, organic acid, free sugar, vitamins and inorganic components. Ginseng saponins are classified as protopanaxadiol (PPD), protopanaxatriol (PPT) and oleanane saponins, depending on the structure of the non-sugar moiety. Among them PPD and PPT are ginseng It is known that Ginseng is a unique saponin and has strong potency such as enhanced immunity and anticancer effect. In the present invention, ginseng can be ginseng which is commonly available in the art, and commercially available ginseng can be used.

Hijikia fusiforme is a perennial seaweed that lives in the lower part of the intertidal zone about 30cm above the low water level and exists only in the metamorphic age. In Korea, it grows from the southern part of Jumunjin to the west coast of Jangsan, and it is widely distributed in South coast and Jeju area. It belongs to the classification system, Heterokontophyta, Phaeophyceae, Fucales, Sargassaceae and Hizikia. It is not only a good source of dietary fiber, Laminaran, a neutral polysaccharide known to have functionalities such as immune enhancing action, and fucoidin, a sulfuric acid polysaccharide, are contained in a large amount. Other antioxidative and antimicrobial activities are also contained. And is known to have excellent efficacy in the prevention and treatment of various adult diseases and chronic diseases. In addition, it has been confirmed that not only cholesterol deposition in blood vessels is prevented, but also intestinal motility is smoothly performed, so that it is effective not only for discharging heavy metals, but also has an anticancer effect. In the present invention, ttot can be a commercially available ttot, and commercially available ttot can be used.

The ginseng and top extracts which are active ingredients in the composition of the present invention include not only a mixture of ginseng extract and top extract but also a mixed extract of ginseng and top. Therefore, the ginseng and top extracts include all of the extracts obtained by extracting ginseng and garnet (or both of the dried or dried product) together, as well as a mixture of extracts obtained by extracting each of them separately.

In the ginseng and top extract of the present invention, the blending ratio of ginseng to ginseng is preferably about 1:10 to 10: 1, more preferably 1: 9 to 9: 1, more preferably 1: : 9 to 3: 1, particularly preferably about 1: 9.

The ginseng and top extract can be prepared by dissolving ginseng and top extract in an extraction solvent selected from the group consisting of water, C1-C4 alcohol, and a mixed solvent of water and C1-C4 alcohol And extracting the solution with an extraction solvent. The primary extraction solvent is preferably an aqueous ethanol solution, more preferably an aqueous 70% ethanol solution. Ginseng and top extracts can also be obtained by extracting ginseng and top from the ginseng extract and top extract, respectively, and then mixing them.

The extraction process may be carried out by extracting from about 1 to about 20 times, preferably about 10 times, the weight of the ginseng and top with the primary extraction solvent, preferably a mixed solvent of water and ethanol. The extraction process may be performed by a known extraction method such as, for example, cold-pressing, hot water extraction, ultrasonic extraction, reflux-cooling extraction, etc., but is not limited thereto. The extraction temperature may be selected by a person skilled in the art from a variety of temperature ranges suitable for the extraction method, for example, but not limited to, from 20 캜 to 100 캜. The extraction time will also vary depending on the extraction method employed and the appropriate extraction time will be within the skill of one of ordinary skill in the art and can be performed in a single or multiple cycles ranging from about 1 hour to 10 days, have. Preferably, the extraction can be carried out by extraction with a primary extraction solvent at about 70 DEG C for about 3 to 5 hours. The extract obtained by performing the extraction with the primary extraction solvent may be obtained in the form of a liquid in which impurities are removed by filtration according to a conventional method, or the obtained liquid form extract may be concentrated under reduced pressure and / Can be obtained.

The extraction step may further include, if necessary, obtaining a fraction having a high active ingredient content. That is, the step of dispersing the extract obtained by extracting with the primary extracting solvent into water and then extracting with an appropriate secondary extracting solvent, for example, alcohol of water-saturated C4, etc., .

The composition of the present invention may contain 0.001 to 99% by weight of ginseng and top (mixed) extract, based on the total weight of the composition.

The composition of the present invention may be a pharmaceutical composition, a food composition or a cosmetic composition.

The composition of the present invention can be used for the prevention, treatment or improvement of alopecia. Alopecia Areata, Androgenetic Alopecia, Telogen Effluvium, Traumatic alopecia, Trichotillomania ), Pressure alopecia, alopecia alopecia, alopecia alopecia, Alopecia syphlltiac, Alopecia seborrhecia, symptomatic alopecia, cicatric alopecia, or congenital alopecia.

In addition, the present invention provides the use of the active ingredient contained in the composition of the present invention for preventing hair loss or promoting hair growth.

The present invention also provides a method for preventing hair loss or promoting hair growth comprising the step of administering the active ingredient contained in the composition of the present invention to a mammal, including a human, in need thereof.

Unless otherwise indicated, the description of compositions, uses, and methods of the present invention are equally applicable, unless they are mutually contradictory.

As described above, the present invention provides a pharmaceutical composition for prevention or treatment of alopecia or alopecia, which comprises ginseng and top extract as an active ingredient.

The pharmaceutical composition may further comprise a pharmaceutically acceptable carrier in addition to the active ingredient. The pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups and aerosols, external preparations and sterilized injection solutions according to a conventional method. The pharmaceutically acceptable carrier may be selected from the group consisting of lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, Cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like.

It also includes diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants, and the like. Solid form preparations for oral use include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose ), Gelatin and the like, and may include a lubricant such as magnesium stearate, talc, and the like. Oral liquid preparations include suspensions, solutions, emulsions, syrups, and the like, and may contain diluents such as water and liquid paraffin, wetting agents, sweetening agents, fragrances, preservatives and the like. Examples of the non-aqueous solution include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions and freeze-dried preparations, and non-aqueous solvents and suspensions include vegetable oils such as propylene glycol, polyethylene glycol and olive oil, And the like.

In the composition, use and method of the present invention, the dosage of the active ingredient varies depending on the condition and body weight of the patient, the degree of disease, the drug form, the administration route and the period, but may be appropriately selected depending on the case. For example, it can be administered at a dose of 0.0001 to 100 mg / kg, preferably 0.01 to 100 mg / kg per day on the basis of the above ginseng and top extract, and the administration can be administered once a day or divided into several times have. In addition, the composition may contain ginseng and top extract in an amount of 0.001 to 50% by weight based on the total weight of the composition. The pharmaceutical compositions may be administered to mammals, such as humans, by a variety of routes, for example, by oral, intravenous, intramuscular, or subcutaneous injection.

The composition of the present invention may be administered or applied locally. Topical application or topical application means direct application of the active ingredient (and composition) to the skin and / or hair. The topical compositions of the present invention may be in the form of solutions, lozenges, pads, creams, ointments, liposomes, sprays, gels, foams, roller sticks, or any other formulations routinely used in dermatology have.

As described above, the present invention provides a food composition for preventing hair loss or promoting hair growth, comprising ginseng and top extract as an active ingredient.

The formulation of the food composition of the present invention may be prepared by a conventional method and may be formulated in the form of capsules or other tablets, granules, powders, beverages, porridge, etc. after drying together with the carrier. .

The food composition of the present invention can be used as a health functional food. "Health functional food" refers to foods prepared and processed using raw materials or ingredients having useful functions in the human body pursuant to Law No. 6727 on Health Functional Foods, and the term "functional" It is intended to take nutrients for the purpose of controlling nutrients and obtaining effects that are useful for health use such as physiological action.

The food composition of the present invention may contain conventional food additives and, unless otherwise specified, the suitability of the food additives as "food additives" is to be determined by the Food and Drug Administration in accordance with General Regulations and General Test Methods approved by the Food and Drug Administration. Shall be determined according to the relevant standards and standards.

Examples of the substances described in the above-mentioned "Food Additive Code" include natural compounds such as ketones, chemical compounds such as glycine, potassium citrate, nicotinic acid and cinnamic acid, persimmon extracts, licorice extract, crystalline cellulose, high- - Mixed preparations such as a sodium glutamate preparation, a noodle-added alkaline agent, a preservative preparation, a tar coloring agent and the like.

The health functional food composition of the present invention can be used in various foods and beverages for prevention of hair loss or promoting hair growth and can be used for various foods, beverages, gums, tea, vitamin complex, health functional auxiliary food, And may be in the form of powders, granules, tablets, capsules, rings or beverages. May be in any food form other than those described above.

INDUSTRIAL APPLICABILITY As described above, the present invention provides a cosmetic composition for preventing hair loss or promoting hair growth comprising ginseng and top extract as an active ingredient.

The cosmetic composition may be prepared in various forms according to a conventional cosmetic preparation method using the ginseng and top extract. For example, the cosmetic composition may be manufactured in the form of a cosmetic product containing ginseng extract and top extract, a shampoo, a hair lotion, a hair cream, a hair gel, a hair tonic, a shampoo, a rinse, a soap, It can be diluted with a normal cleansing liquid, a converging liquid, and a moisturizing liquid. In addition, the cosmetic composition may contain conventional auxiliaries or cosmetically acceptable carriers such as stabilizers, solubilizers, vitamins, pigments, and perfumes which are commonly used in the field of cosmetic compositions.

The carrier for cosmetics includes, but is not limited to, purified water, oils, waxes, fatty acids, fatty acid alcohols, fatty acid esters, surfactants, moisture absorbers, thickeners, viscosity stabilizers, chelating agents, buffers, preservatives, It is not. If necessary, moisturizing agents, anti-inflammatory agents, antibacterial agents, antifungal agents, vitamins, sunscreen agents, antibiotics, perfumes, and dyes may be included.

The active ingredient contained in the composition of the present invention can be formulated by adding a pharmaceutically or pharmacologically acceptable carrier, excipient or diluent. For formulation, Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA et al.

The active ingredients of the composition of the present invention, ginseng and capsicum extract, have the activity of promoting the growth of human dermal papilla cells and human keratinocytes. As a result, hair growth and healthy scalp growth can be promoted. Therefore, the composition of the present invention is useful as a pharmaceutical composition, a food composition, or a cosmetic composition for alopecia suppressing or promoting growth of wool / hair growth and hair of hair, and for preventing hair loss or promoting hair growth.

FIG. 1 shows the results of measuring the cell growth promoting effect upon treatment of human dermal papilla cells (HHDPC) with ginseng and top extracts ((-): negative control, MXD: positive control, combination 1-5: 1-1 to 1-5).
FIG. 2 shows the results of measuring the cell growth promoting effect of ginseng and top extract on human keratinocyte (HaCaT cell) ((-): negative control, MXD: positive control, combination 1-5: Examples 1-1 to 1-5).
FIG. 3 shows the result of measuring the hair growth promoting effect when treating ginseng and top extract with C57BL / 6 mouse.
FIG. 4 shows histological changes of ginseng and top extracts treated with C57BL / 6 mouse.

Hereinafter, the present invention will be described in more detail through Examples, Test Examples and Preparation Examples. However, these examples, test examples and preparation examples are for illustrating the present invention, and the scope of the present invention is not limited thereto.

[Example]

≪ Example 1 > Preparation of ginseng and top extract (70% ethanol extract)

1 kg of domestic ginseng and natural dried gins purchased from Kum Kang Pharmaceutical Co., Ltd. (Nakdong, Korea) was washed three times in distilled water to remove impurities and salts on the surface, dried naturally for 48 hours, and then dried at 20-40 mesh The ground ginseng product and the ground product were mixed in the ratio shown in Table 1, respectively, to obtain a mixture of the ginseng ground product and the ground product.

[Table 1]

The mixture of the ginseng ground product and the ground product was subjected to reflux extraction at 70 ° C for 4 hours after adding 10 times 70 vol.% Ethanol (EtOH). After extraction, the mixture was filtered through a filter paper (4 μm, watman) and concentrated under reduced pressure at 45 ° C. for 6 to 12 hours using a vacuum concentrator (Coolace CCA-1100, manufactured by EYELA) After removal of ethanol (EtOH), a concentrate was obtained to prepare ginseng and top mixed extracts of Examples 1-1 to 1-5.

[ Test Example ]

≪ Test Example 1 > Confirmation of hair loss prevention effect or hair growth promoting effect of ginseng and top extracts - Evaluation of effects on growth of human hair dermal papilla cells

The effects of ginseng and top extracts on the cell growth of human papillary cells were evaluated using human hair dermal papilla cells (HHDPC) (Sciencell, cat. No. 2400).

The cells were inoculated to a 96-well plate in a medium of MSC (Mesenchymal Stem Cell) containing 10% FBS (fetal bovine serum, Cambrex) such that HHDPC was 0.25 × 10 ⁴ cells / % CO ₂ and incubated under humid conditions. The cultures were treated with 1 ug / ml of the mixed extracts of Ginseng and Tarts of Examples 1-1 to 1-5 for 24, 48, and 72 hours, and 5 mg / ml of MTT (3- (4 , 5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolinium bromide) reagent was added to the culture solution and cultured at 37 ° C under 5% CO ₂ wet condition for 4 hours. As a negative control, the culture medium treated with 10 μM of minoxidil (Hyundai Pharm) was used as a positive control, and the culture medium treated with 200 μl of the cell culture medium was used as a negative control. In culture, purple crystals form in living cells. After 4 hours of incubation, the medium was removed, and 100 μl of DMSO was added. The crystal was dissolved in a shaking incubator for 10 minutes, and the absorbance was measured at 540 nm to measure the degree of cell growth. The above test was repeated three times, and the obtained results were as shown in Fig.

As shown in FIG. 1, the ginseng and green tea extracts showed an improvement of about 21.2 ~ 46.4% in the growth of the papillary cells, and exhibited better activity of promoting the induction of hair cell induction than the minoxidil treated group. In the case of Example 1-2 (Formulation 2), it exhibited particularly excellent hair inducing cell growth promoting activity. From the above results, it can be seen that the ginseng and top extract, which are active ingredients contained in the composition of the present invention, have an effect of preventing hair loss or promoting hair growth.

≪ Test Example 2 > Confirmation of hair loss prevention effect or hair growth promoting effect of ginseng and top extracts - Evaluation of effect on human keratinocyte (HaCaT cell) growth

The effect of ginseng and top extract on the cell growth effect of keratinocytes was evaluated by the same method as in Test Example 1 except that horny cells (HaCaT cell) (Dr. NE Fuseing, Heidelberg, Germany) Respectively.

The medium was RPMI medium containing 10% FBS (fetal bovine serum, Cambrex), and the results obtained from the MTT analysis were as shown in Fig.

As shown in FIG. 2, the extracts of ginseng and top extracts showed an increase in keratinocyte growth of about 24.4 ~ 53.3% and exhibited keratinocyte growth promoting activity superior to that of the positive control group, minoxidil. In the case of Example 1-2 (Formulation 2), it showed particularly excellent keratinocyte growth promoting activity. From the above results, it can be seen that the ginseng and top extract, which are active ingredients contained in the composition of the present invention, have an effect of preventing hair loss or promoting hair growth.

≪ Test Example 3 > Confirmation of hair loss prevention effect or hair growth promotion effect of ginseng and top extracts - Evaluation of hair growth effect in C57BL / 6 mice

Seven-week-old C57BL / 6 mice were divided into four groups (group A-D) in which four to five mice were removed from the dorsal area of the dorsal area. Groups A to C were treated with a Wax-Rosin mixture to disrupt the hair follicles, followed by application of saline solution for 18 days (negative control group), and group B received 5 mg / kg (Positive control group), and the group C was prepared by dissolving the Example 1-2 (Compound 2) prepared in Example 1 in saline and applying it at a dose of 5 mg / kg for 18 days. The results of measuring the distribution of hair on days 8 and 12 were as shown in FIG.

In order to observe changes in hair follicles, skin thickness, and texture of the skin, mice of each group were sacrificed on days 4, 8, and 12, and tissues of the hairy areas of the dorsal skin were collected And fixed with 4% formaldehyde solution. After fixing, washing with water, dehydration, embedding with paraffin, and cutting of 7 μm, hematoxylin and eosin (H & E) staining were performed. Histological changes were observed as shown in FIG.

As can be seen from Figs. 3 and 4, the skin color of the mouse treated with the ginseng and green tea extract was changed to black in a short period of time and the hair quickly grows. The number of hair follicles in the skin tissue significantly increased, It was confirmed to be maintained. As a result, it was confirmed that the ginseng and green tea extracts exhibited an excellent hair growth effect and were safe substances without skin irritation.

[Formulation Example]

≪ Formulation Example 1 > Preparation of pharmaceutical composition

100 mg of ginseng and top extract, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate prepared in the same manner as in Example 1 were filled in a gelatin capsule to prepare a capsule.

≪ Formulation Example 2 > Preparation of food composition

(4 wt.%), Liquid fructose (0.5 wt.%), Oligosaccharide (2 wt.%), Sugar (2 wt.%) And salt (0.5 wt.%) Prepared in the same manner as in Example 1 Were added, and the remaining amount was adjusted, and homogeneously blended and instant sterilized to prepare a health drink.

≪ Formulation Example 3 > Preparation of cosmetic composition

1. Manufacture of nutrition lotion

Nutritional lotion containing ginseng and top extract prepared in the same manner as in Example 1 was prepared by the following method with the contents shown in Table 2 below.

[Table 2]

The raw materials 10, 11, 13, and 15 in the above table were heated to 80 to 85 degrees Celsius while being mixed and stirred, and charged into an emulsifier. The raw materials 2, 3, 4, 5, 6, 7, 8, And the mixture was emulsified by charging it into an emulsifier by heating the emulsion. After the emulsification is completed, the raw material 12 is cooled to 50 ° C with stirring using a stirrer, and then the raw material 14 is added thereto, cooled to 35 ° C and then cooled to 25 ° C. .

2. Manufacture of nutritional cream

Table 3 shows compositions for preparing nutritional creams containing ginseng and top extracts.

[Table 3]

Water, purified water, triethanolamine and glycol (1,3-Propanediol) are heated and dissolved at 70 DEG C, and a fatty acid, an oily component, an emulsifier and an antioxidant are heated to 70 DEG C to dissolve. After the emulsification is completed, the solution is cooled to 45 ° C, and ginseng and top extracts and fragrance are added and dispersed, followed by cooling to 30 ° C.

Claims (9)

Wherein the ginseng extract is a mixture of ginseng extract and top extract, or a mixed extract of ginseng and top extract. The pharmaceutical composition according to claim 1, wherein the ginseng extract is selected from the group consisting of Panax ginseng and Hijikia fusiforme extract. The pharmaceutical composition according to claim 1, which is for the prevention or treatment of alopecia. 3. The method of claim 2, wherein the alopecia are selected from the group consisting of alopecia areata, hereditary alopecia alopecia, resting alopecia, traumatic alopecia, hairy wall, compression alopecia, alopecia alopecia, non-rigorous alopecia, alopecia alopecia, , Or congenital alopecia. Wherein the ginseng and top extract is a mixture of ginseng extract and top extract, or a mixed extract of ginseng and tortoise. The composition for promoting hair loss or promoting hair growth comprises the extract of Panax ginseng and Hijikia fusiforme as an active ingredient. The food composition according to claim 4, which is for preventing or ameliorating alopecia. 6. The method of claim 5, wherein the alopecia are selected from the group consisting of alopecia areata, hereditary alopecia, traumatic alopecia, traumatic alopecia, bullae, pressurized alopecia, alopecia alopecia, alopecia alopecia, syphilis alopecia, alopecia alopecia, , Or congenital alopecia. Ginseng (Panax ginseng) and fusiforme (Hijikia fusiforme) extracts containing as an active ingredient, wherein the ginseng and fusiforme extract is ginseng extract and the mixture of fusiformis extract, or a mixed extract of hair loss prevention or hair growth cosmetic composition for promoting the ginseng and Chop. The cosmetic composition according to claim 7, which is for preventing or ameliorating alopecia. 9. The method of claim 8, wherein the alopecia are selected from the group consisting of alopecia areata, hereditary alopecia, alopecia alopecia, traumatic alopecia, hairy wall, pressurized alopecia, alopecia alopecia, non-rigic alopecia, alopecia alopecia, , Or congenital alopecia.

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