KR101913737B1 - A solid fermentation composition from caragana and vegetable worms and a functional cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a solid fermentation product of a Chinese pea tree, Caragana spp., And a vegetable worm, and a functional cosmetic composition comprising the same. More specifically, the present invention relates to a functional fermented product comprising a caterpillar fungus A solid fermentation product obtained by solid fermentation by inoculation, and a functional cosmetic composition for promoting antioxidation, skin aging prevention and skin regeneration comprising the solid fermentation product. The fermented bean curd and caterpillar fungus solid fermented product of the present invention is a composition derived from a natural product having no cytotoxicity, and is excellent in antioxidation, inhibition of skin aging and promotion of skin regeneration, and thus can be used as a functional cosmetic composition for antioxidant and anti- Which is very useful in the cosmetics industry.

Description

FIELD OF THE INVENTION The present invention relates to a solid fermented product of Cordyceps militaris and to a functional cosmetic composition containing the same and a functional cosmetic composition containing the same. BACKGROUND ART < RTI ID = 0.0 >

The present invention relates to a solid fermentation product of a Chinese pea tree, Caragana spp., And a vegetable worm, and a functional cosmetic composition comprising the same. More specifically, the present invention relates to a functional fermented product comprising a caterpillar fungus A solid fermentation product obtained by solid fermentation by inoculation, and a functional cosmetic composition for promoting antioxidation, skin aging prevention and skin regeneration comprising the solid fermentation product.

Skin is a part of the body that is directly exposed to the external environment. It is exposed to ultraviolet rays caused by exposure to sunlight in daily life, air pollutants such as yellow dust or automobile soot, and various bacteria, thereby causing skin trouble and irritation. . Such aging of the skin reduces the elasticity of the skin on the appearance of the skin to form wrinkles or to form spots and senile spots to cause skin pigmentation.

In general, aging of the skin is accompanied by various theories such as somatic mutation, excessive production of free radicals, autoimmune reaction, accumulation of toxic substances, errors generated during gene replication, transformation of dendritic proteins, And the diseases caused by various diseases such as adult diseases are attributed to reactive oxygen species (ROS) is recognized that the active oxygen species originating from oxygen and nitrogen to control the natural antioxidants to develop active research is progressing have. The reactive oxygen species, usually known as harmful oxygen, is a lipid peroxide such as peroxide anion radical, hydrogen peroxide, hydroxy radical, peroxidized lipid or free radical generated in the most stable form, It occurs even under normal conditions, but the presence of antioxidant enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and the like inhibit the production of excessive reactive oxygen species. However, when the active oxygen species is abnormally excessive due to stress and external environmental factors and is not eliminated, the oxidative stress due to the free radicals is added to the living body and DNA, RNA, protein, Cell membrane and cell structure damage, cancer, tissue damage, and aging.

Studies on antioxidants capable of controlling or eliminating these active oxygen species have been actively conducted in order to suppress the rapid increase of various diseases and aging in the modern age. Studies on the efficacy of natural antioxidants such as tocopherol, ascorbic acid, carotenoids, flavonoids and glutathione, and their stabilization techniques, and studies on the synthetic antioxidants butylated hydroxytoluene (BHT), butylated hydroxyanisol (BHA), and many other antioxidants have been continuously studied. Among these antioxidants, tocopherol has a relatively low antioxidative effect, and synthetic antioxidants such as BHA and BHT, which are highly effective, are used in pharmaceuticals and foods, but there is a point of mutagenicity and toxicity, and in the case of ascorbic acid, Development of the obtained derivatives and formulations is progressing, and development of a natural antioxidant material having more stable and effective effect is urgently required.

In order to solve such a problem, many studies have been made on a cosmetic composition using an extract which is relatively low in toxicity, safe and effective for skin, among phytochemicals, It has been required to develop a material which maximizes its efficacy since the effect of the ingredient is limited.

On the other hand, in the case of natural extracts through fermentation, sugar components present in natural products are converted into alcohols as carbon sources in the metabolic process of microorganisms without using harmful organic solvents, so that the active ingredients in natural products, especially flavonoids, Polyphenols, catechins, etc. are present as glycated and stable compounds, and the extractability of the active ingredient is increased through fermentation, and excellent skin efficacy is expected by the active ingredient which is excluded from sugar and activated.

Cordyceps is a mushroom in the winter and a mushroom in the summer. The spores and mycelium of the fungus, a kind of fungus, invade into living insects and lurk during winter. Is an odd mushroom that causes insects to die in summer and uses endogenous materials as a nutrient source to produce endosynthetic sclerotia and then to form fruit bodies outside the body of insects.

There have been more than 750 kinds of Cordyceps in the world so far and more than 100 species such as Cordyceps spp., Paecilomyces spp, Isaria spp. do.

According to Chinese ancient books, Cordyceps is not only effective in cancer, liver disease, lung disease, brain disease, cardiovascular disease, heart disease, diabetes, etc., but it also has pulmonary tuberculosis, elderly debilitating chronic tuberculosis anemia, . Recently, it has been reported that tumor suppression rate is 83%, which is an anticancer effect and antidote to drug poisoning, and it has natural healing power and it is reported that recovery is effective when heavy physical exercise is heavy.

It is a plant that grows in mountainous area. The appearance of the plant spreads in all directions, and its stem is grayish brown. It is a plant mainly known for ornamental use of about 2m in height with five ridges.

The leaves are alternate phyllotaxis, odd numbered iguate compound leaf, 4 small ovate leaves, 1 flower in May, 2.5 ~ 3cm long, 2.5 ~ 3cm long, and the calyx is bell-shaped, the upper half is yellowish red, Half is light yellow, peduncle is about 1cm long, fruit is round, round, with no hairs, rips in 3 ~ 3.5cm length in September.

It is a plant that is often planted in the garden for ornamental purposes. It is said to have dried roots in one room, and it is called osteoporosis (bone 擔 根). It has the efficacy of analgesia and sore throat, and is prescribed in seawater, small bowel, hypertension, bruise, neuralgia It is distributed in Korea (Gyeongsangbuk-do, Kyonggi-do, Gangwon-do, Hwanghae-do) and China.

KR 10-2010-0027291 A KR 10-2012-0068312 A

DISCLOSURE OF THE INVENTION It is an object of the present invention to solve the problems of the prior art as described above, and it is an object of the present invention to solve the above problems. And a functional cosmetic composition using the solid fermented product.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems, and provides a solid fermented product of a mushroom and Cordyceps, which is prepared by inoculating and cultivating a mycelia of Cordyceps mushroom on sterilized Mushroom.

The fungus is coordinating sepseu sinen system (Cordyceps sinensis), Cody sepseu Milla other leases (Cordyceps militaris), Cody sepseu Scarab Cola (Cordyceps scarabaecola), Cody sepseu press Tansu (Cordyceps nutans), Cody sepseu seupeko Seppala (Cordyceps sphecocephala) , Cordyceps tricentri , Paecilomyceps tenuipes , and Paecilomyces japonica . ≪ IMAGE >

The culture is preferably performed under conditions of a temperature of 20 to 35 DEG C, a relative humidity of 70 to 90%, and a culture period of 7 to 45 days.

In addition, the present invention provides a cosmetic composition comprising the fermented product of the scallop and the caterpillar fungus of the present invention.

The cosmetic composition preferably has a function selected from the group consisting of antioxidation, skin aging prevention, and skin regeneration promotion.

The cosmetic composition of the present invention can be used in cosmetics such as a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, , A lipstick, a shadow, a shampoo, and a rinse.

The fermented bean curd and caterpillar fungus solid fermented product of the present invention is a composition derived from a natural product having no cytotoxicity, and is excellent in antioxidation, inhibition of skin aging and promotion of skin regeneration, and thus can be used as a functional cosmetic composition for antioxidant and anti- Which is very useful in the cosmetics industry.

Fig. 1 shows a process for producing a fermented product of Cordyceps sinensis extract of the present invention.
Fig. 2 shows the SOD radical scavenging activity of the fermented product of Cordyceps sinensis extract of the present invention.
Fig. 3 shows the Xanthine oxidase assay results of the fermented product of Cordyceps sinensis extract of the present invention.
FIG. 4 is a northern blot diagram showing the expression pattern of SIRT1 (Sirtuin1) gene of the fermented product of Cordyceps sinensis extract of the present invention.
FIG. 5 is a graph showing the SIRT1 (Sirtuin1) gene expression pattern of the fermented product of Cordyceps sinensis extract of the present invention.
FIG. 6 shows the skin wound regeneration effect of the fermented product of Cordyceps sinensis extract of the present invention over time.
Figure 7 shows the NRU phototoxicity results of CPZ (chloropromazine), known as a phototoxic substance.
Fig. 8 shows the NRU phototoxicity results of the fermented solid of Cordyceps sinensis extract of the present invention.
FIG. 9 shows the results of MTT assay of the fermented product of Cordyceps mellonus Cordyceps sinensis according to the present invention.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention have conducted intensive studies to develop materials for functional cosmetic compositions using natural products. As a result, it has been found that the fermented product of Cordyceps Cordyceps sinensis obtained by inoculating the Cordyceps mushroom mycelium with the mushroom, and culturing it according to the solid culture method, Skin aging and skin regeneration activity, and suggest that the fermented product of the present invention can be used as a functional cosmetic composition.

Accordingly, the present invention provides a solid fermented product of a mushroom and Cordyceps, which is prepared by inoculating and cultivating a mycelia of Cordyceps mellifera with a sterilized mushroom.

The Cordyceps sinensis according to the present invention is a fungus belonging to the Cordyceps mellifera of the Acanthopanax senticosus, and specifically includes Cordyceps spp., Paecilomyces spp., Isaria spp. ( Phytocordyceps spp.), And more than 750 species are known worldwide.

In the present invention, all of these Cordyceps sinensis can be used, and preferably Cordyceps sinensis , Cordyceps militaris , Cordyceps scarabaecola , Codisps mutans, Cordyceps nutus , Cordyceps sphecocephala , Cordyceps tricentri , Paecilomyceps tenuipes , and Paecilomyces japonica . , More preferably Paecilomyces japonica, also referred to as Snowcone Cordyceps.

The mycelium of Cordyceps mellifera of the present invention can be produced by seed culture of Cordyceps spp.

As a preferable example, the mycelia of Cordyceps sinensis are inoculated into a PDA (Potato Dextrose Agar) medium, and then subcultured at about 4 weeks intervals at a temperature of about 25 ° C. .

The seed culture is a process for culturing the stored mycelium of Cordycepian mycelium prior to the main culture. In a preferred embodiment, the seed culture can be obtained as a liquid culture by inoculating seed culture, i.e., mycelium, into a culture medium for mushroom mycelium, and then culturing the culture in a rotary shake.

The bivalvar falciparum of the present invention may be any kind of plant of the genus Caracana ( Caragana spp.), But preferably it may be C. sinica . In the present invention, the whole of the bamboo plant can be used, but preferably it can be selected from the group consisting of fruit, seed, stem, leaf and root, more preferably selected from fruit, stem or leaf And most preferably leaves.

Sterilization of the bamboo shoots can be performed by washing the prepared bamboo shoots, dehydrating them, drying them, and applying various known sterilization methods. In a preferred embodiment, the sterilization can be autoclaved at 121 DEG C for 30 minutes.

The method of inoculating the mycelium of Cordyceps mushroom mycorrhizae of the present invention into sterilized bamboo shoots is aseptically inoculating a sterilized bamboo shoot with a certain amount of the liquid culture obtained by the above-described bug culture.

The cultivation is preferably performed at a temperature of 20 to 35 ° C, a relative humidity of 70 to 90%, and a cultivation period of 7 to 45 days. As a preferred example, the cultivation is carried out at a temperature of 25 ° C In an incubator with a relative humidity of 80% for 30 days.

The solid fermented product of Cordyceps mellifera, which has been fermented, is in the form that the surface of Cordyceps sinensis is completely covered with the mycelium of Cordyceps mellifera, and thus an extract of the fermented product of Cordyceps mellifera can be produced.

The solvent used for the extraction may be water or lower ethanol, but is preferably hot water extraction. As a preferred embodiment, the obtained fermented product of Cordyceps celadon herbaceae can be subjected to supernatant by adding hot water of about 5 to 150 times volume, shaking for 1 to 5 hours, and centrifuging. The above process may be performed 1 to 5 times, preferably 3 times.

The solid fermented product or the extract thereof of the present invention can be prepared by the above-mentioned concrete manufacturing process, and it can be applied as the material of the functional cosmetic composition described below.

Accordingly, the present invention provides a functional cosmetic composition comprising the fermented product of Cordyceps sinensis extract of the present invention.

The term " functional " means an activity selected from the group consisting of antioxidation, prevention of skin aging, and promotion of skin regeneration.

The main component of the cosmetic composition of the present invention may be 0.01 to 50% by weight, preferably 0.1 to 20% by weight, more preferably 1 to 10% by weight based on the total weight of the composition. It is possible to secure appropriate formulation stability in such a content range and to anticipate the effect of the desired antioxidation, prevention of skin aging and promotion of skin regeneration.

The composition of the present invention may further include known natural extracts or other ingredients for antioxidation, skin aging prevention, and promotion of skin regeneration in addition to solid fermented products of Cordyceps mellifera have.

In addition, the composition may also contain components commonly added to cosmetic compositions, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, A cosmetic or dermatological composition such as a perfume, a perfume, a surfactant, water, an ionic or nonionic type emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent May be formulated into a particular formulation, including adjuvants or carriers commonly used in the field of dermatology.

The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be formulated into various forms such as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, Containing cleansing, oil and spray, but are not limited thereto. More specifically, the present invention relates to a cosmetic composition for cosmetics such as a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a cleansing cream, a cleansing foam, a cleansing water, Rose, lipstick, shadow, shampoo, and rinse.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. The following examples illustrate one preferred embodiment of the present invention and are not to be construed as limiting the scope of the present invention.

[Example]

1. Preparation of sample

1.1. Preparation of Hot Water Extract of Solid Fermented Water from Cordyceps Cordyceps

Paecilomyces Japonica was cultivated using a PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 25 ° C using a strains under cultivation in Palm Bios Co., . In a 500 ml flask, 100 ml of mushroom mycelial solid medium (1 cm x 1 cm, 3 pieces) was inoculated with the mycelia of the snowy Chinese mushroom mycelium in a volume of 100 ml, and then inoculated in a rotary shaking incubator (Daihan Labtech Co.Korea, 25 ± 1 ° C, 130 rpm) Day, and used as liquid seeds.

The goryodan was purchased from the Daegu herb medicine market and washed with water before washing. The gorydum was dehydrated after removing impurities. 100 g of the goryodum was put in a 3 L culture container, sterilized at 121 캜 for 30 minutes under high pressure and then cooled at room temperature. Were inoculated aseptically into 100 g of sterilized red bean curd. Fermentation was carried out for 30 days in an incubator at 25 ° C and 80% relative humidity. After fermentation, 10 times volume of hot water was added, and the mixture was shaken for 2 hours and then immersed for 72 hours. After centrifugation at 10,000 rpm for 10 minutes, the supernatant was taken. After repeating this three times, the extract was concentrated under reduced pressure to prepare a powder Respectively.

1.2. Preparation of hot water extract of mushroom solid fermented water

A hot-water extract of the fermented product of Mulberry mushroom was prepared by the same method as the preparation of the hot-water extract of the fermented product of Fermented Cordyceps mellifera of the above item 1.1, except that the mushroom was used instead of the mulberry.

1.3. Preparation of Hot Water Extract of Fermented Solid Fermented Mushrooms

The fermented hot water extract of Mulberry fermented with Mulberry was prepared by the same method as the preparation of the hot water extract of the fermentation product of the mulberry mushroom,

2. Activity evaluation

2.1. Antioxidant activity verification

The antioxidant activity of the fermented product of the above-prepared Fermented Cordyceps mellifera / Situ mushroom / Ganoderma lucidum was evaluated. (1, 1-diphenyl-2-picryl hydrazyl) radical scavenging activity and FRAP activity were measured in the fermented solid fermented product (0.5 mg / ml)

For DPPH scavenging ability, 380 μl of 2 × 10 ^-4 M DPPH solution dissolved in 99.5% ethanol was added to 20 μl of diluted samples at various concentrations, and the mixture was reacted at 37 ° C. for 30 minutes. Then, microplate reader (Asys Hitech, Expert 96, Asys Co., Austria). DPPH free radical scavenging activity was expressed as a percentage as shown in Table 1 below.

[Table 1] DPPH radical scavenging activity

The FRAP activity was carried out according to a known method for the prepared Fermented Tobacco Cordyceps mellifera / Mushroom / Ganoderma lucidum fermented product (0.5 mg / ml).

[Table 2] FRAP activity

2.2. Cell Regeneration / Aging Suppression Test

2.2.1. Elastase inhibitiory assay

The results of evaluating the elastase inhibitory activity (%) for the solid fermented products of Cordyceps mellonus / Situ mushroom / Ganoderma lucidum prepared were shown in Table 3 below.

[Table 3] Elastase inhibitory assay

Based on the above results, elastase inhibitory activity (%) was measured by concentration (30, 100, 300 and 1,000 μg / ml) and compared with positive control EGCG (epigallocatechin gallate) I could.

2.2.2. Superoxide dismutase assay

The SOD inhibition activity of three solid fermented products subjected to antioxidation test and Elastase inhibitiory assay such as DPPH and FRAP showed that the SOD inhibitory activity was increased in the extracts fermented with Cordyceps sinensis and Matsutake mushroom than that of 300 μg / (Fig. 2).

2.2.3. Xanthine oxidase assay

As a result of xanthine oxidase assay of three kinds of solid fermented products, the activity of xanthine oxidase tended to decrease according to the concentration of bamboo shoot extract (FIG. 3). Especially, in the fermented product of Cordyceps mellifera, about 30% more inhibitory effect was observed in the solid fermented product than in the control, but the solid fermented product of mushroom and gingko mushroom showed weak inhibitory effect than the solid fermented product of Cordyceps. As a result, xanthine oxidase inhibition activity by solid fermentation was varied depending on the samples, but it was generally improved through fermentation, and it was confirmed that the fermented product had the most excellent activity in the solid fermented product.

2.2.4. Identification of SIRT1 gene expression

SIRT1 has been shown to increase the lifespan by delaying proliferative aging by altering transcriptional activity to restore DNA damage, with resistance to oxidative attack while inhibiting apoptosis and proliferation through deacetylation. By confirming the expression level of SIRT1 gene, it was confirmed whether or not the fermented bean curds had an effect on aging. Positive control used resveratrol, which is known to activate the SIRT1 gene.

As shown in FIG. 4 and FIG. 5, when SIRT1 gene expression level of the fermented product of Cordyceps sinensis Cordyceps sinensis was measured, it was lower than that of resveratrol but higher than that of untreated group, . From the above results, it is anticipated that the solid fermentation of Mt.

2.2.5. Wound healing assay

The wound healing assay was performed on human fibroblast BJ cells. The wound healing after 14 hours of BJ cell treatment with Bombyx mori was not complete but showed some therapeutic effect. On the other hand, the solid fermentation products of Cordyceps mellifera, Cordyceps mongolica and Ganoderma lucidum increased the degree of wound healing compared to the extracts of Bombyx mori. These results show that the results of the measurement of elastase inhibitory activity showed a similar tendency to that of the solid fermented product of Cordyceps mellifera, which showed excellent elastase inhibitory activity and STIR1 gene expression, and these results are related to each other (FIG. 6).

2.3. (3T3 NRU phototoxicity test)

The NRU phototoxicity of the fermented bean curd solids was measured using CPZ (chloropromazine) known as a phototoxic substance as a positive control using NIH / 3T3 cells (FIGS. 7 and 8). The MPE value and the PIF value were found to be 0.118 and 7.057, respectively, and the PIF value was found to be higher than 5. The MPE value was 0.007 and the PIF value was 13.815. Which is phototoxic. The MPE value was 0.297 and the PIF value was 1.890, and MPE> 0.15 was found to be phototoxic. On the other hand, in the extract of Cordyceps sinensis, the MPE value was 0.010 and the PIF value was 1.000, and it was determined that there was no phototoxicity in the range of MPE <0.1 and PIF <2. These results suggest that the extracts of Bombyx mori L. can prevent phototoxicity through solid fermentation using Cordyceps sinensis and protect the skin from ultraviolet rays.

2.4. Cytotoxicity test (MTT assay)

In order to evaluate the cytotoxicity of the solid fermentation product of Cordyceps militaris, the cell viability (%) was measured by MTT assay using BJ cells, and the results are shown in FIG. The cell viability decreased to less than 80% when the concentration was higher than 300 μg / ml. In the case of the solid fermented product, the cell viability of the mushroom fermented product and the mushroom fermented product of the mushroom tended to decrease with increasing concentration. However, in the case of the mushroom fermented product, the cell survival rate was not decreased up to 1,000 μg / ml I did.

Claims (6)

A functional cosmetic composition for the improvement of wound healing, comprising a solid fermented product of cormorant and Cordycepsis prepared by inoculating and cultivating a mycelia of Cordyceps mellifera with a sterilized corolla. The method of claim 1, wherein the fungus is coordinated sepseu sinen sheath (Cordyceps sinensis), coordination sepseu Millar other less (Cordyceps militaris), coordination sepseu Scarab coke (Cordyceps scarabaecola), coordination sepseu press Tansu (Cordyceps nutans), coordination sepseu seupeko Seppala (Cordyceps sphecocephala), coordination sepseu tree Sentry (Cordyceps tricentri), L indeed My process Te sister Fes (Paecilomyceps tenuipes) and L indeed functional cosmetic composition being selected from the group consisting of my process japonica (Paecilomyces japonica) . The functional cosmetic composition according to claim 1, wherein the culture is performed at a temperature of 20 to 35 DEG C, a relative humidity of 70 to 90%, and a culture period of 7 to 45 days. The cosmetic composition according to claim 1, wherein the functional cosmetic composition is at least one selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a cleansing cream, a cleansing foam, a cleansing water, , Powder, fact, lip gloss, lipstick, shadow, shampoo and rinse. delete delete

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