JP3934017B2 - Cosmetics - Google Patents

Description

【００５０】
表1に示したように、いずれの菌株の培養液も高い活性を示した。特にハイイロシメジ培養液は、4.31と高いマンガンパーオキシダーゼ活性を示した。
【００５１】
マンガンパーオキシダーゼの調製およびメラニンの分解
培養液100mlに硫酸アンモニウム58ｇを加え、溶解させる。4℃で1昼夜放置し、沈殿を生じさせる。3,000rpm、10分間遠心分離により、沈殿を回収し、乾燥して粗酵素粉末を得る。この粉末を精製水10mlに溶解し、酵素溶液とする。調製した酵素溶液5mlに0.1g/mlイカスミメラニン（グリコ栄養食品社製）分散水溶液を50μlを添加し、室温で3日間放置した。その後、メラニンをろ過した溶液の400nmの吸光度を測定し、メラニンの分解度を測定した。
【００５２】
【表２】
各種菌株から精製した酵素溶液のメラニン分解度

【００５３】
表２に示したように、いずれの菌株から調製した酵素溶液もメラニンを分解し、400nmにおける吸光度の値が上昇していることがわかった。陰性対象として酵素液の代わりに精製水を用いたものは、400nmの吸光度値はほとんど増加は認められなかった。特にハイイロシメジから調製した酵素溶液は、3.85と高い値を示し、メラニン分解が進んでいた。また、表1との比較から、マンガンパーオキシダーゼ活性の高いものは、メラニン分解性も高いことが認められた。
【００５４】
次に、本発明の各種成分を配合した化粧料の処方例の例を示すが、本発明はこれに限定されるものでない。
化粧料の処方例
【００５５】
（１）化粧用クリーム （重量％）
a)ミツロウ・・・2.0
b)ステアリルアルコール・・・5.0
c)ステアリン酸・・・8.0
d)スクワラン・・・10.0
e)自己乳化型グリセリルモノステアレート・・・3.0
f)ポリオキシエチレンセチルエーテル(20E.O.)・・・1.0
g)ヒラタケ培養液・・・10.0
h)シャクヤク抽出液・・・0.5
i)アスコルビン酸グルコシド・・・2.0
j)1,3-ブチレングリコール・・・5.0
k)水酸化カリウム・・・0.3
l)防腐剤・酸化防止剤・・・適量
m)精製水・・・残部
製法 a)〜f)までを加熱溶解し、80℃に保つ。H)〜m)までを加熱溶解し、80℃に保ち、a)〜f)に加えて乳化し、40℃まで撹拌しながら冷却する。その後、g)を加え、攪拌し均一に溶解する。
【００５６】
（２）乳液 （重量％）
a)ミツロウ・・・0.5
b)ワセリン・・・2.0
c)スクワラン・・・8.0
d)ソルビタンセスキオレエート・・・0.8
e)ポリオキシエチレンオレイルエーテル(20E.O.)・・・1.2
f)ハイイロシメジ培養液・・・15.0
g)イレイセンエキス・・・2.0
h)1,3-ブチレングリコール・・・7.0
i)カルボキシビニルポリマー・・・0.2
j)水酸化カリウム・・・0.1
k)精製水・・・残部
l)防腐剤・酸化防止剤・・・適量
m)エタノール・・・7.0
製法 a)〜e)までを加熱溶解し、80℃に保つ。h)〜l)までを加熱溶解し、80℃に保ち、a)〜e)に加えて乳化し、50℃まで撹拌しながら冷却する。50℃でf)、g)、m)を添加し、40℃まで攪拌、冷却する。
【００５７】
（３）化粧水 (重量%)
a)キクラゲ培養液乾燥物・・・0.1
b)アスコルビン酸リン酸マグネシウム・・・1.0
c)グリセリン・・・5.0
d)ポリオキシエチレンソルビタンモノラウレート(20E.O.)・・・1.0
e)エタノール・・・6.0
f)香料・・・適量
g)防腐剤・酸化防止剤・・・適量
h)精製水・・・残部
製法 a)とｂ）を均一に混合する。ｃ)〜h)までを混合し、均一に溶解する。使用時に2剤を混合して使用する。
【００５８】
（４）化粧水 (重量%)
a)マンガンパーオキシダーゼ・・・0.01
b)グルタチオン・・・0.1
c)グリセリン・・・5.0
d)ポリオキシエチレンソルビタンモノラウレート(20E.O.)・・・1.0
e)エタノール・・・6.0
f)香料・・・適量
g)防腐剤・酸化防止剤・・・適量
h)精製水・・・残部
製法 a)〜b)までを混合する。ｃ)〜h)までを混合し、均一に溶解する。使用時に2剤を混合して使用する。
【００５９】
（５）洗顔剤 (重量%)
a)ヒラタケ培養液乾燥物・・・0.1
b)アスコルビン酸グルコシド・・・2.0
c)シャクヤク抽出液・・・0.5
d)タルク・・・残部
e)セルロース・・・20.0
f)ミリスチン酸カリウム・・・30.0
g)ラウリルリン酸ナトリウム・・・10.0
h)香料・・・適量
i)防腐剤・・・適量
製法 a)〜i)までを混合し、よく撹拌、分散させ均一にする。
【００６０】
【効果確認試験】
（１）塗布によるヒトでの効果確認試験
被験者として、２０〜５０歳の女性15名に１日２回（朝、夜）連続２ヵ月間、本発明品と比較品のそれぞれを使用させ、塗布部位の状態を試験前後で比較し、改善効果を調べた。本試験には、試験品として【００５５】、【００５９】で示した化粧料を用い、対照品 1 には【００５５】、【００５９】に示した化粧料からヒラタケ培養液を除いた化粧料、対照品 2 には【００５９】に示した化粧料からシャクヤク抽出液、アスコルビン酸グルコシドを除いた化粧料を作成し、その使用による効果について調べた。 本発明の有効成分を配合した化粧料を毎日使用しながら肌のクスミおよび美白効果を塗布開始前及び2ヶ月塗布後におけるアンケートで集計し、効果の確認を行った。 結果は表３に示す。表３からも明らかなように、従来の美白成分のみからなる対照品 1 、メラニン分解性物質のみからなる対照品 2 と比較して、美白成分とメラニン分解性物質からなる試験品は高い効果が認められた。
【００６１】
【表３】

【００６２】
【発明の効果】
以上詳述したごとく、本発明の化粧料は、角質中のメラニンを分解し、かつ同時に配合される美白成分によってメラニン産生を抑制するため、高い美白効果を有するものである。[0001]
[Industrial application fields]
The present invention contains a melanolytic substance and a whitening component, thereby decomposing melanin produced in melanocytes and then transferred into the stratum corneum with a melanolytic substance and producing new melanin. The present invention relates to a whitening cosmetic having an unprecedented high effect by suppressing with a whitening component.
[0002]
[Prior art]
Melanin is biosynthesized in the central nerve and retina in addition to the skin, and is widely distributed in nature from plants to microorganisms, not limited to animals. Melanin is produced by the conversion of tyrosine to dopa and further dopaquinone by the action of tyrosinase, followed by oxidation to indole-5,6-dihydroquinone, which is polymerized.
[0003]
The biosynthesis of melanin takes place in melanocytes present in the basal layer of the epidermis. In melanocytes, melanin is synthesized and matured in granules called melanosomes, migrates and disperses into epidermal cells, and finally becomes plaque and falls off. Melanin plays an important role in protecting the body from the harmful effects of ultraviolet rays and is an important medical factor. However, if the amount of melanin is too large, it becomes dark skin, which is a serious cosmetic problem such as rash, stain, buckwheat.
[0004]
Currently known countermeasures against Kusumi, blemishes, and freckles include a method of shielding the ultraviolet rays that trigger melanin biosynthesis with a sunscreen agent, and substances that inhibit melanin biosynthesis, such as arbutin, kojic acid, and vitamins. Methods using melanin synthesis inhibitors such as C derivatives and plant extracts that suppress melanin biosynthesis are known.
[0006]
However, the present condition is that the safe method without the side effect for decomposing | disassembling and disappearing the melanin in the stratum corneum once formed has not been developed yet. In the past, hydroquinone, 4-isopropylcatechol, and hydroquinone monobenzyl ether have been used as skin bleaching agents, but these have strong depigmenting effects, but they are based on degeneration and lethality of pigment cells. However, if it is used continuously, it becomes permanent vitiligo, and side effects such as rash are unavoidable, so it is not currently marketed as a cosmetic. In this industry, it is a safe whitening agent effective for Kumi, stains and freckles. Development was strongly desired.
[0007]
Therefore, at present, in order to accelerate the period in which melanin falls off with the renewal of the epidermis for Kusumi, blemishes, and buckwheat, the use of placental extracts that have a metabolic promoting effect and the softening of keratin such as resorcin and salicylic acid Only a method for shortening the turnover of the epidermis using a release agent is known. However, this method is not inherently a fundamental treatment for Kusumi, blemishes and buckwheat.
[0008]
[Problems to be solved by the invention]
The present invention has been made in view of the current state of the art, and by preventing the formation of kusumumi, blemishes, freckles with whitening ingredients, and by decomposing melanin in the stratum corneum, kusumumi, blemishes, It was made for the purpose of developing whitening cosmetics that show an unprecedented high whitening effect against buckwheat.
[0009]
[Means for Solving the Problems]
In order to achieve the above object, the present invention has been completed by combining a melanin-decomposing substance and a whitening component, focusing on wood-rotting fungi having melanin decomposability among microorganisms.
[0010]
As the microorganisms that can be used in the present invention, wood-rotting fungi having melanin decomposability can be used, for example, pleurotus, Pholiota, Lentinus, Auricularia, oyster mushroom Basidiomycetes belonging to the genus (Clitocybe), Pseudohiatula, and Phellinus can be used.
[0011]
More specifically, among basidiomycetes belonging to the genus Pleurotus, oyster mushrooms (Pleurotus ostreatus (Jacq.:Fr.)Kummer), bamboo shoots (Pleurotus cornucopiae (Paulet) Rolland var. Citrinopileatus (Sing.) Ohira), Pleurotus salmoneostramineus L. Vass., Pleurotus pulmonarius (Fr.) Quel, is a basidiomycete belonging to the genus Pholiota, Pholiota aurivella (Batschmer Fum. (Pholiota adiposa (Fr.) Kummer), Nameko (Pholiota nameko (T.Ito) S. Ito et Imai inImai), Nagaritake (Pholiota flammans (Fr.) Kummer), Sugitake (Pholiota squarrosa (Mull Fr.) Kummer), Pholiota squarrosoides (Peck) Sacc., Phaniota lubrica (Pers. Fr.) Sing. Is a basidiomycete belonging to the genus Pinus genus Lentinus edodes (Berk.) Sing., Matsuouji (L entinusFr.em Sing.) is a basidiomycete belonging to the genus Asterus (Auricularia auricula (Hook.) Underw.), Auricularia jellyfish (Auricularia polytricha (Mont.) Sacc.), However, in basidiomycetes belonging to the genus Clitocybe, Clitocybenebularis (Batsch: Fr.) Kummer, Clitocybe odora (Bull: Fr.) Kummer, Hotito-shimeji (Clitocybe clavipes (.Pers) Fr. Kummer) is a mosquito (Clitocybe gibba (Pers: Fr.) Kummer), a basidiomycete belonging to the genus Pseudohiatula is Pseudohiatulaoshimae, and a basidiomycete belonging to the genus Phellinus is P.yucatensis (Murr.) Imaz. Available.
[0012]
The melanolytic substance used in the present invention can be produced by culturing mycelia of basidiomycetes. Basidiomycetes can induce mycelia from natural fruiting bodies and soil, and can also be sold from strain distribution agencies. By culturing these mycelium, a melanin-degrading substance such as manganese peroxidase is produced in the culture solution. Manganese peroxidase is known as an enzyme that degrades wood lignin, and is used for pretreatment of wood saccharification, decolorization of dyes, decolorization of colored waste water, and decomposition of hardly decomposable substances.
[0013]
About the plant extract in the whitening component utilized in this invention, the seed | species of a plant is not specifically limited. It includes everything that is called generically.
[0014]
Licorice is a perennial plant belonging to the genus Licorice, a plant native to the Ussuri region from northern China that is rarely cultivated.
[0015]
Ireisen is a plant belonging to the family Dendrobaceae, also called ginseng.
[0016]
Sunflower is an annual plant belonging to the genus Sunflower, which is widely cultivated for ornamental use and oil extraction.
[0017]
Ibukitorano is a perennial plant belonging to the genus Ibukitrano, which is distributed in Hokkaido, Honshu, Shikoku and the northern hemisphere from the cold zone to the temperate zone and grows in sunny grasslands in the mountains.
[0018]
Ages is a deciduous shrub of the rose family, also known as a rose. It grows widely in Japan.
[0019]
Ogon is a perennial plant belonging to the family Lamiaceae and is also referred to as Scotch. Native to China, Korea and Siberian wilderness.
[0020]
Kujin is a perennial of the bean family, also known as Clara. It occurs in the wilderness and roadside.
[0021]
Gokahi is a deciduous shrub of the family Araceae and grows naturally in the sunny hills of Yamano. Use root bark and stem bark. It is also called Yamaukogi or Onikougi.
[0022]
Saishin is a perennial plant belonging to the genus Usbasaicin, a suzuma family of horses, that ripens in the shade of the mountains of Honshu and Kyushu.
[0023]
Hawthorn is a deciduous shrub belonging to the family Hawthorn, which is cultivated for ornamental purposes.
[0024]
Shirayuri is a perennial plant belonging to the genus Lily family, and is cultivated at home.
[0025]
Peonies are perennials belonging to the genus Buttonaceae and are planted for ornamental or medicinal purposes.
[0026]
Sempukuka is a perennial perennial plant belonging to the asteraceae family, and grows naturally near the fields and hills of the hilly plains that are sunny and moist. Also called Ogulma.
[0027]
Sohakuhi is a deciduous Takagi tree that grows naturally in mountains throughout Japan.
[0028]
Soybean is an annual plant of the legume family.
[0029]
Tea is an evergreen shrub of the camellia family.
[0030]
Japanese cypress is a perennial plant of the sericulture family and is cultivated in various locations.
[0031]
Byaclen is a vine belonging to the genus Grapeaceae.
[0032]
Beech trees are deciduous Takagi trees belonging to the genus Beechidae, which form a pure forest in the temperate zone of southwestern Hokkaido and Kyushu.
[0033]
Grapes are deciduous vines belonging to the genus Grapeaceae. It is widely planted as a fruit in the temperate zone of the world.
[0034]
A hop is a perennial vine in the family Hoeaceae.
[0035]
Maikaika is a rose family plant and is also called Hermanus. Wild on the beach in Hokkaido.
[0036]
Yukinoshita is a perennial plant belonging to the genus Yukinoshita, and is distributed from Honshu to Kyushu and the warm temperate zone of China. Fly over wet rocks in the mountains.
[0037]
Yokuinin is an annual plant of the grass family and is also called pearl barley. Native to tropical Asia and cultivated in various parts of Japan.
[0038]
Further, kojic acid and its derivatives which are whitening components and salts thereof, ellagic acid and its derivatives and salts thereof, endothelin antagonist, ascorbic acid and its derivatives and salts thereof, glutathione and its derivatives and salts thereof, cysteine Commercially available reagents can be used for these and derivatives thereof, and salts thereof, resorcin and derivatives thereof, and salts thereof, hydroquinone and derivatives thereof, and salts thereof. Various solvents from plants containing a large amount of these compounds, such as water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol; acetone Extraction using 1 type or 2 types or more of alkyl esters such as ethyl acetate; hydrocarbons such as benzene and hexane; ethers such as diethyl ether; halogenated alkanes such as dichloromethane and chloroform; It can be used after purification. Furthermore, the above compound can be prepared by chemical synthesis.
[0039]
In addition, grabrizine, glabrene, liquiritin, isoliquiritin and licorice extract, placenta extract, carotenoids and animal and plant extracts containing these, irasen extract, sunflower seed extract, ibukitorano extract, agetsu extract , Ogon extract, Ononis extract, Seaweed extract, Kujin extract, Sesame extract, Oil containing linoleic acid, Oil containing linolenic acid, Saicin extract, Hawthorn extract, Shirayuri extract, Peonies extract , Sempukuka extract, Sowakuhihi extract, soybean extract, tea extract, Toki extract, sandalwood extract, beech extract, grape seed extract, hop extract, mica extract, saxifrage extract, yokuinin extract The preparation is not particularly limited. For example, various suitable organic solvents can be used. It is extracted under heating from a low temperature to have. Examples of the extraction solvent include water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol, and 1,3-butylene glycol; ketones such as acetone and methyl ethyl ketone; and ethyl acetate. One or more of alkyl esters; hydrocarbons such as benzene and hexane; ethers such as diethyl ether; and halogenated alkanes such as dichloromethane and chloroform can be used. In particular, one, two or more mixed solvents of water, ethyl alcohol, and 1,3-butylene glycol are particularly suitable.
[0040]
Production of melanolytic substances consisting of basidiomycetes of the genus Pleurotus, Pholiota, Lentinus, Auricularia, Clitocybe, Pseudohiatula, and Phellinus Can be used after culturing mycelium in potato-dextrose medium, malt extract medium, Chapec-Dox medium, Ebios medium, yeast extract medium, Sabouraud medium, oatmeal medium, etc., and then drying the culture solution as it is. In addition, peroxidases present in the culture solution are salted out with salts such as ammonium sulfate. The substance precipitated by salting out may be dried and used as it is, but it can be used after further purification if necessary. The amount of the purified peroxidase in the cosmetic of the present invention is preferably 0.00001 to 10.0% by weight, and particularly preferably 0.001 to 0.1% by weight.
[0041]
Moreover, it is also possible to filter the said culture solution and to mix | blend with cosmetics as it is. In this case, the blending amount can be used in the range of 0.01 to 100% by weight, and preferably in the range of 10.0 to 50.0% by weight.
[0042]
Kojic acid and its derivatives and their salts, ellagic acid and their derivatives and their salts, endothelin antagonists, ascorbic acid and their derivatives and their salts, glutathione and their derivatives and their salts, cysteine and their used as whitening ingredients Derivatives and salts thereof, resorcin and derivatives thereof, and salts thereof, hydroquinone and derivatives thereof, and salts thereof, glabrizine, glabrene, liquiritin, isoliquiritin, and licorice extract, placenta extract, carotenoids and the like containing these Animal and plant extracts, Ireisen extract, sunflower seed extract, Ibukitorano extract, Agetsu extract, Ogon extract, Ononis extract, seaweed extract, Kudin extract, Gokahi extract, fat and oil containing linoleic acid, Reno Fats and oils containing acid, Saishin extract, Hawthorn extract, Shirayuri extract, Peonies extract, Sempukuka extract, Sakuhakuhi extract, soybean extract, tea extract, Toki extract, sandalwood extract, beech extract , Grape seed extract, hop extract, mica squid extract, yukinoshita extract, yokoinin extract, each material is dried and then finely pulverized 1 to 1000 times by weight, especially 10-100 It is preferable to use a double amount of solvent at 0 ° C. or higher, particularly 20 ° C. to 40 ° C. for 1 hour or longer, particularly 3 to 7 days. Moreover, you may heat-extract at 60-100 degreeC for 1 hour.
[0043]
Each of the above-mentioned extracts obtained under the above conditions may be used as an extracted solution. However, if necessary, it is necessary to appropriately use a concentrated and powdered product after filtration or the like. I can do it.
[0044]
The blending amount of the whitening component in the cosmetic of the present invention is preferably 0.00001 to 20.0% by weight in terms of the evaporated and dried content, and particularly the range of 0.01 to 10.0% by weight is optimal.
[0045]
In addition to the above essential components, the cosmetics of the present invention are aqueous components, oily components, plant extracts, animal extracts, powders, excipients, surfactants, oils, alcohols, pH adjusters, preservatives, antioxidants. It is prepared by mixing agents and thickeners, sweeteners, pigments, fragrances, and the like as necessary and blending appropriately. The dosage form of the cosmetic of the present invention is not particularly limited, and can be various dosage forms such as lotion, milky lotion, cream, pack, powder, spray, ointment, dispersion, and cleaning agent.
[0046]
【Example】
Examples of melanin degradation of various products according to the present invention and elimination of Kusumi / stain / sobacas by melanin production inhibitory effects are shown below, and examples of prescriptions applied to cosmetics using the materials are described here. Needless to say, the present invention is not limited to these examples.
[0047]
Cultivation of wood-rotting fungi As wood-rotting fungi, oyster mushroom (IFO: No.30776), numerisugitake mushroom (IFO: No.30265), shiitake mushroom (IFO: No.30719.), Jellyfish (IFO: No.5949.), The strains purchased from the Fermentation Research Institute were used for gray shimeji (IFO: No. 9350) and Pseudohiatula oshimae (IFO: No. 30370). Meshimakobu is aseptically excised from a natural fruiting body and placed on a potato-dextrose agar medium prepared from Potato-Dextrose Broth (Difco) 2.4% and agar 1.5%. After 2 weeks of culture, the mycelium obtained was used. As the medium, a yeast medium prepared by dissolving 3.0 g of yeast extract, 3.0 g of polypeptone, 3.0 g of malt extract, and 1.0 g of sucrose in 1 L of purified water was used. The culture was performed by adding 100 ml of a culture solution to a 200 ml Erlenmeyer flask, sterilizing, planting mycelia, and rotating culture at 24 ° C. and 150 rpm for 10 days.
[0048]
Determination of manganese peroxidase titer
Add 1 ml of 2,6-dimethoxyphenol (2,6-DMP) 1% aqueous solution to 2 ml of culture medium, 0.3 ml of buffer solution prepared to pH 4.5 with 0.2 M-citrate-NaOH, 0.7 ml of purified water, and 10 minutes The subsequent change in absorbance at 475 nm was measured.
[0049]
[Table 1]
Manganese peroxidase activity in various bacterial cultures

[0050]
As shown in Table 1, the culture solution of any strain showed high activity. In particular, the gray shimeji broth showed a manganese peroxidase activity as high as 4.31.
[0051]
Preparation of Manganese Peroxidase and Melanin Decomposition Add 100 g of ammonium sulfate to 100 ml of the culture solution and dissolve. Leave at 4 ° C for 1 day to cause precipitation. The precipitate is collected by centrifugation at 3,000 rpm for 10 minutes and dried to obtain a crude enzyme powder. This powder is dissolved in 10 ml of purified water to make an enzyme solution. To 5 ml of the prepared enzyme solution, 50 μl of a 0.1 g / ml squid mimelanin (Glyco Nutrition Foods) dispersed aqueous solution was added and left at room temperature for 3 days. Thereafter, the absorbance at 400 nm of the solution obtained by filtering melanin was measured, and the degree of degradation of melanin was measured.
[0052]
[Table 2]
Degradation of melanin in enzyme solutions purified from various strains

[0053]
As shown in Table 2, it was found that the enzyme solution prepared from any strain decomposed melanin and the absorbance value at 400 nm increased. In the case of using purified water instead of the enzyme solution as a negative target, the absorbance value at 400 nm hardly increased. In particular, the enzyme solution prepared from gray shimeji mushroom showed a high value of 3.85, and melanin decomposition was advanced. Further, from comparison with Table 1, it was confirmed that those having high manganese peroxidase activity also had high melanolytic activity.
[0054]
Next, although the example of the formulation example of the cosmetics which mix | blended various components of this invention is shown, this invention is not limited to this.
Cosmetic formulation example [0055]
(1) Cosmetic cream (wt%)
a) Beeswax ... 2.0
b) Stearyl alcohol ... 5.0
c) Stearic acid: 8.0
d) Squalane ... 10.0
e) Self-emulsifying glyceryl monostearate ... 3.0
f) Polyoxyethylene cetyl ether (20E.O.) ... 1.0
g) Oyster mushroom broth ... 10.0
h) Peonies extract ... 0.5
i) Ascorbic acid glucoside ... 2.0
j) 1,3-butylene glycol ... 5.0
k) Potassium hydroxide ... 0.3
l) Preservatives / Antioxidants ... appropriate amount
m) Purified water: The rest of the manufacturing method a) to f) is dissolved by heating and kept at 80 ° C. Heat up to H) to m), keep at 80 ° C., add to a) to f), emulsify, and cool to 40 ° C. with stirring. Then g) is added and stirred to dissolve uniformly.
[0056]
(2) Emulsion (wt%)
a) Beeswax ... 0.5
b) Petrolatum ... 2.0
c) Squalane ... 8.0
d) Sorbitan sesquioleate ... 0.8
e) Polyoxyethylene oleyl ether (20E.O.) ... 1.2
f) Gray shimeji broth ... 15.0
g) Ireisen extract ... 2.0
h) 1,3-butylene glycol ... 7.0
i) Carboxyvinyl polymer ... 0.2
j) Potassium hydroxide ... 0.1
k) Purified water: balance
l) Preservatives / Antioxidants ... appropriate amount
m) Ethanol ... 7.0
The process up to production methods a) to e) are dissolved by heating and kept at 80 ° C. Heat up to h) to l), keep at 80 ° C., add to a) to e), emulsify, and cool to 50 ° C. with stirring. Add f), g), m) at 50 ° C., stir to 40 ° C. and cool.
[0057]
(3) Lotion (wt%)
a) Dried fungus culture ... 0.1
b) Magnesium phosphate ascorbate ... 1.0
c) Glycerin ... 5.0
d) Polyoxyethylene sorbitan monolaurate (20E.O.) ... 1.0
e) Ethanol ... 6.0
f) Perfume ... appropriate amount
g) Preservatives / Antioxidants ...
h) Purified water: Remaining preparation method a) and b) are mixed uniformly. c) to h) are mixed and dissolved uniformly. Mix two agents when using.
[0058]
(4) Lotion (wt%)
a) Manganese peroxidase: 0.01
b) Glutathione ... 0.1
c) Glycerin ... 5.0
d) Polyoxyethylene sorbitan monolaurate (20E.O.) ... 1.0
e) Ethanol ... 6.0
f) Perfume ... appropriate amount
g) Preservatives / Antioxidants ...
h) Purified water: Mix the remaining manufacturing methods a) to b). c) to h) are mixed and dissolved uniformly. Mix two agents when using.
[0059]
(5) Facial cleanser (wt%)
a) Oyster mushroom broth dried product ... 0.1
b) Ascorbic acid glucoside ... 2.0
c) Peonies extract ... 0.5
d) Talc ... remainder
e) Cellulose ... 20.0
f) Potassium myristate ... 30.0
g) Sodium lauryl phosphate ... 10.0
h) Perfume
i) Preservatives: Appropriate amount of production method a) to i) are mixed, stirred and dispersed well to make uniform.
[0060]
[Effectiveness confirmation test]
(1) Human effect confirmation test by application As a test subject, 15 women of 20 to 50 years old use the product of the present invention and the comparative product twice a day (morning and night) for 2 consecutive months. The condition of the part was compared before and after the test, and the improvement effect was investigated. In this test, the cosmetics indicated by [0055] and [0059] were used as test products, and the control product 1 was a cosmetic obtained by removing the oyster mushroom culture solution from the cosmetics indicated by [0055] and [0059] For Control 2, a cosmetic prepared by removing the peony extract and ascorbic acid glucoside from the cosmetic shown in [0059] was prepared, and the effects of its use were examined. While using cosmetics containing the active ingredient of the present invention daily, skin smears and whitening effects were tabulated before and after application for 2 months to confirm the effects. The results are shown in Table 3. As is clear from Table 3, the test product consisting of the whitening component and the melanin-degrading substance is more effective than the control product 1 consisting only of the conventional whitening component and the control product 2 consisting only of the melanin-degrading substance. Admitted.
[0061]
[Table 3]

[0062]
【The invention's effect】
As described in detail above, the cosmetic of the present invention has a high whitening effect because it decomposes melanin in the stratum corneum and suppresses melanin production by the whitening component blended at the same time.

Claims (2)

A cosmetic comprising essentially a cultured and / or processed product and a whitening component of Clitocybene cruciforme (Batsch: Fr.) Kummer . The whitening component is kojic acid and its derivatives and their salts, ellagic acid and its derivatives and their salts, endothelin antagonist, ascorbic acid and its derivatives and their salts, glutathione and its derivatives and their salts, cysteine and its Derivatives and salts thereof, resorcin and derivatives thereof, and salts thereof, hydroquinone and derivatives thereof, and salts thereof, glabrizine, glabrene, liquiritin, isoliquiritin and licorice extract, placenta extract, carotenoids containing these and the like Animal and plant extracts, Ireisen extract, Sunflower seed extract, Ibukitorano extract, Agetsu extract, Ogon extract, Ononis extract, Seaweed extract, Kudin extract, Gokahi extract, Oil containing linoleic acid, Linolenic acid Oils and fats, Saisin extract, Hawthorn extract, Shirayuri extract, Peonies extract, Sempukuka extract, Sakuhakuhi extract, Soybean extract, Tea extract, Toki extract, Juniper extract, Beech tree extract, Grape seed extract The cosmetic according to claim 1 , wherein the cosmetic is one or more selected from a product, a hop extract, a mica squid extract, a Yukinosita extract, and a Yokuinin extract.