KR101906245B1 - Cosmetic composition for anti-oxidant and skin whitening effect comprising buckwheat seed extract of specific germination condition as effective component - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening and antioxidation comprising an effective amount of buckwheat seed extract germinated with a shoot having a length of 4 to 6 cm. The present invention relates to a cosmetic composition for skin whitening and antioxidation, The present invention relates to a cosmetic composition for skin whitening and antioxidation comprising methanol extract of bitter buckwheat seeds germinated with seeds and shoots 5 to 6 cm in length as an active ingredient, The cosmetic composition for skin whitening and antioxidation comprising methanol extract of bitter buckwheat seeds germinated with soybean buckwheat seeds and shoots 5 to 6 cm in length has excellent skin whitening effect and antioxidative effect, It is expected that it can be usefully used as a cosmetic.

Description

Technical Field [0001] The present invention relates to a skin whitening and antioxidative cosmetic composition comprising buckwheat seed extract having a specific germination state as an active ingredient,

The present invention relates to a cosmetic composition for whitening skin and antioxidation comprising buckwheat seed extract having a specific germination state as an active ingredient.

Buckwheat is an annual plant belonging to the family Polygonaceae . Buckwheat ( Fagopyrum esculentum Moench) and bitter buckwheat ( Fagopyrum tataricum Gaertner) are grown all over the world. However, buckwheat and bitter buckwheat differ in many respects. But buckwheat is self-fertilized while buckwheat is the other. The shape of the seed is also different, but the buckwheat is a triangular rhombus, and the bitter buckwheat has an oval shape. It is known that bitter buckwheat has a higher content of rutin, bitter taste and better efficacy than sweet buckwheat. It is known that soybean buckwheat grows about 70 days, but bitter buckwheat is 14 ~ 21 days longer than buckwheat.

Studies on the superiority of buckwheat nutrients and the components and efficacy of flavonoids have been reported in various countries. Buckwheat protein is higher than rice, rye, corn and sugar cane. In addition, the fatty acid composition of buckwheat has been reported to be significantly higher than that of wheat or barley in terms of the content of essential fatty acids which are related to prevention of adult diseases. The minerals content of buckwheat is high in the shell part of seeds, and buckwheat, rather than buckwheat, contains higher minerals content. The vitamin composition of buckwheat is high in vitamin B1, B2, and niacin, and is twice as high as that of rice and wheat.

Buckwheat has long been widely used as a medicinal product. In the basics of the meal, buckwheat is said to show stomach and intestines, to lift up energy, to cleanse the mind, and to remove the dregs of the five. In addition, it says that buckwheat eliminates red ginseng and eliminates heat and gout. Such buckwheat has traditionally been used for measles, ulcerative gastric diseases, chest pain, hemorrhagic diseases, and also for bruises and boils.

Buckwheat has flavonoid compounds such as rutin, orientin, isoorientin, vitexin, and isovitexin. It has been reported that buckwheat, rather than plain buckwheat, is rich in flavonoid compounds, especially routines. Buckwheat is effective in treating circulatory diseases, strengthening capillaries, and anti-inflammation, and is known to exhibit anti-allergic, diuretic and anti-inflammatory actions. In addition, it is known that it is effective for diabetes and arteriosclerosis.

Germination occurs when the seeds of a plant induce useful substances and is regulated by environmental signals such as light, temperature, and soil components necessary for germination. The seeds store nutrients before germination begins. They store nutrients such as starch, protein, and lipids for enzyme and hormone activities in seed embryos. Seed germination changes morphologically. Seed absorbs moisture and the embryo is elongated, and the root is passed through the seed coat. The development of the seed depends on the formation of the organ due to cell division and differentiation. After the activation of enzymes, the degradation of storage tissues, and the movement of nutrients to the growth point, the seed coat is ruptured due to the increase of the inner pressure due to the increase of the size of the posterior axis and the growth point is started. It usually occurs in the order of roots and buds, and eventually begins to absorb water and photosynthesis, resulting in an independent nutritional system. This germination process is accompanied by a change in the physiologically active component in the seed as well as an external change in the plant.

Korean Patent Laid-Open Publication No. 2016-0000093 discloses an antioxidative and whitening cosmetic composition containing an extract of germinated grains as an active ingredient. In Korean Patent Laid-Open No. 2015-0027427, there is disclosed an antioxidative and anti-inflammatory Discloses a cosmetic composition containing a buckwheat sprout extract having a whitening and wrinkle-improving activity. However, as in the present invention, cosmetic compositions for skin whitening and antioxidation containing a specific germinated buckwheat seed extract as an active ingredient have been disclosed Not at all.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-described needs, and it is an object of the present invention to provide a method of producing a fagopyrum esculentum Moench) seeds and shoots of 5 to 6 cm in length, including bitter buckwheat ( Fagopyrum Gaertner tataricum) was the most excellent in the methanol extract of the seeds DPPH scavenging activity and tyrosinase inhibitory ability, at this time was confirmed that the total polyphenol content of the routine, the orientation of tin, iso-orientation of tin, non teksin and iso bitek new face the highest point flavonoid content. Therefore, the present invention can be applied to cosmetic compositions for skin whitening and antioxidation, in which the sweet buckwheat seed extract germinated with a shoot length of 4 to 5 cm and the bitter buckwheat seed extract germinated with a shoot having a length of 5 to 6 cm By confirming, the present invention has been completed.

In order to attain the above object, the present invention provides a cosmetic composition for skin whitening comprising as an active ingredient a buckwheat seed extract germinated with a shoot having a length of 4 to 6 cm.

The present invention also provides an antioxidative cosmetic composition comprising as an effective ingredient a buckwheat seed extract germinated with shoots having a length of 4 to 6 cm.

In addition, the present invention provides a pharmaceutical composition for preventing or treating skin pigmentation diseases, comprising buckwheat seed extract germinated with shoots having a length of 4 to 6 cm as an active ingredient.

The present invention also provides a food composition for skin whitening comprising, as an active ingredient, an extract of buckwheat seeds germinated with a shoot having a length of 4 to 6 cm.

The present invention relates to a cosmetic composition for skin whitening and antioxidation comprising methanol extract of bitter buckwheat seeds germinated with a sweet soba seed germinated at 4 to 5 cm in length and buds of 5 to 6 cm in length as an active ingredient The present invention relates to a method for skin whitening and antioxidation comprising methanol extracts of bitter buckwheat seeds germinated at a length of 5 to 6 cm, The cosmetic composition has excellent skin whitening effect and antioxidant effect and is expected to be useful as a cosmetic for whitening and anti-aging.

FIG. 1 shows the DPPH scavenging activity (%) of the buckwheat and bitter buckwheat seeds as germination progressed. (A) sweet buckwheat, (B) bitter buckwheat
FIG. 2 shows the results of investigation of total polyphenol content (mg / g) of short buckwheat and bitter buckwheat seed as germination progressed. (A) sweet buckwheat, (B) bitter buckwheat
FIG. 3 shows the results of investigation of the mushroom tyrosinase activity (%) of short buckwheat and bitter buckwheat seeds as germination progressed. (A) sweet buckwheat, (B) bitter buckwheat
4A and 4B are graphs showing the results of HPLC analysis of the content of five flavonoid compounds (orienting, isoordantine, bitescic, isovichecin and lutin in the order of peak) including roots of short buckwheat seeds as germination proceeds to be.
5A and 5B are graphs showing the results of HPLC analysis of the contents of five flavonoid compounds (orienting, isoordantin, bitescic, isovichecin, and rutin in the order of peak) including the roots of bitter buckwheat seeds as germination progressed to be.

In order to accomplish the object of the present invention, the present invention provides a cosmetic composition for skin whitening comprising an extract of buckwheat seed germinated as a shoot having a length of 4 to 6 cm as an active ingredient.

In the present invention, 'whitening' refers to inhibiting, inhibiting or alleviating hyperpigmentation of the skin. Hyperpigmentation of the skin includes freckles, stains, hyperpigmentation after exposure to ultraviolet light, hyperpigmentation after inflammation, aging black spots, brown spots or black spots.

In the cosmetic composition for skin whitening according to an embodiment of the present invention, the extract may be water, methanol, ethanol, propanol, butanol or a mixed solvent thereof, preferably methanol extract, but is not limited thereto. The extract is most preferably a methanol extract of bitter buckwheat seeds germinated with 4-5 cm long germinated sole buckwheat seeds or buds 5-6 cm long, but is not limited thereto.

In the cosmetic composition for skin whitening according to an embodiment of the present invention, the buckwheat may be selected from the group consisting of Fagopyrum esculentum Moench) or bitter buckwheat ( Fagopyrum , < / RTI > tataricum Gaertner).

In the skin whitening cosmetic composition according to an embodiment of the present invention, the content of the extract may be 0.0001% to 10% based on the total weight of the composition, but is not limited thereto.

In the cosmetic composition for skin whitening according to an embodiment of the present invention, buckwheat seeds germinated at a shoot length of 4 to 6 cm have superior DPPH scavenging ability and tyrosinase inhibition ability compared with seedless seeds, and have a total polyphenol content It may have a high content of flavonoids, such as rutin, orientin, isoorientin, bitexin and isovitectin.

The extract according to an embodiment of the present invention may inhibit tyrosinase activity and inhibit melanin production.

The cosmetic composition for skin whitening according to one embodiment of the present invention can be widely used for cosmetics and cleanser having a skin whitening effect, and is particularly suitable for skin external ointment, cream, softening longevity, nutritional lotion, pack, essence, Shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation nutrition But are not limited to, one type of formulation selected from the group consisting of essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The formulation of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a humectant, a fragrance, a fragrance carrier, a protein, a solubilizing agent, a sugar derivative, a sunscreen, a vitamin, a plant extract and the like.

When the cosmetic composition for skin whitening as described above is formulated into pharmaceuticals or cosmetics, it may contain known excipients applicable to the skin acting as a carrier for the active ingredient. When formulating into pharmaceuticals, reference is made to the disclosure of [Remington ' s Pharmaceutical Science, Mack Publishing Company, Easton PA], and in formulation into cosmetics, International cosmetic ingredient dictionary, 6th ed., The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995).

The cosmetic composition for skin whitening according to an embodiment of the present invention may contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, One or more adjuvants selected from water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, But are not limited thereto.

The present invention also provides an antioxidative cosmetic composition comprising as an effective ingredient a buckwheat seed extract germinated with shoots having a length of 4 to 6 cm.

In the cosmetic composition for antioxidation according to an embodiment of the present invention, the extract may be water, methanol, ethanol, propanol, butanol or a mixed solvent thereof, preferably methanol extract, but is not limited thereto. The extract is most preferably a methanol extract of bitter buckwheat seeds germinated with 4-5 cm long germinated sole buckwheat seeds or buds 5-6 cm long, but is not limited thereto.

In the cosmetic composition for antioxidation according to an embodiment of the present invention, the buckwheat is selected from the group consisting of Fagopyrum esculentum Moench) or bitter buckwheat ( Fagopyrum , < / RTI > tataricum Gaertner).

In addition, the present invention provides a pharmaceutical composition for preventing or treating skin pigmentation diseases, comprising buckwheat seed extract germinated with shoots having a length of 4 to 6 cm as an active ingredient.

In the pharmaceutical composition for preventing or treating skin pigmentation diseases according to an embodiment of the present invention, the skin pigmentation disease occurs locally in the skin due to an increase in the synthesis of melanin pigment, and is caused by stains, freckles, But is not limited to, one or more diseases selected from drug-induced pigmentation, post-inflammatory pigmentation, and hyperpigmentation resulting from dermatitis.

In the pharmaceutical composition for preventing or treating skin pigmentation disease according to an embodiment of the present invention, the treatment and prevention of the skin pigmentation disease may be performed by inhibiting tyrosinase and inhibiting melanin production, but the present invention is not limited thereto.

In the pharmaceutical composition for preventing or treating skin pigmentation disease according to an embodiment of the present invention, the content of the extract may be 0.0001% to 10% based on the total weight of the composition, but is not limited thereto.

In the pharmaceutical composition for the prevention or treatment of skin pigmentation diseases according to an embodiment of the present invention, the pharmaceutical composition for preventing or treating skin pigmentation diseases includes a cream, a gel, a patch, a spray, an ointment, A starch, a starch, a liniment, a pasta or a cataplasma.

The present invention also provides a food composition for skin whitening comprising, as an active ingredient, an extract of buckwheat seeds germinated with a shoot having a length of 4 to 6 cm.

When the buckwheat seed extract of the present invention germinated at a shoot length of 4 to 6 cm is used as a food additive, the extract may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the extract of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, in the production of food or beverage. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. As a specific example, the above-mentioned extract can be used to produce a processed food having good storage properties while utilizing the characteristics of agricultural products, livestock products or aquatic products. Such processed foods include, for example, confectionery, drinks, liquor, fermented foods, canned foods, milk processed foods, meat processed foods, noodles and the like. The sweets include biscuits, pies, breads, candies, jellies, gums, cereals (including dinner utensils such as cereal flakes). Drinks include carbonated beverages, functional ionic beverages, juices (such as apples, pears, grapes, aloes, citrus fruits, peaches, carrots, tomato juices, etc.) and sikhye. The mainstream includes sake, whiskey, shochu, beer, liquor, and fruit wine. Fermented foods include soy sauce, miso, and kochujang. Canned products include canned products (for example, tuna, mackerel, sandwiches, canned fish, etc.), canned products (canned beef, pork, chicken and turkey canned products) and canned products (corn, peach and pineapple canned products). Milk processed foods include cheese, butter, yogurt and the like. Meat processed foods include pork cutlet, beef cutlet, chicken cutlet, sausage, sweet and sour pork, nuggets, nubucki, and the like. And noodles such as sealed packaging raw noodles. In addition, the composition may be used in retort food, soup and the like.

In addition, functional foods, health foods or health supplements can be produced using the above extract. The functional food, the health food or the health supplement food refers to a food which provides a bio-controlling function including a physiologically active ingredient in addition to the nutritional function, and the extract of the present invention has a skin whitening prevention or improvement effect, Health food supplements and the like.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited to the following examples

Materials and methods

1) Experimental material

Buckwheat and bitter buckwheat grown in 2015 in Pyeongchang, Gangwon Province were purchased and stored in a refrigerator at 4 ℃ for the experiment. Lutein, orientin, isoorientin, bitexin and isovitectin used for HPLC analysis were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

2) Germination of buckwheat

However, buckwheat and bitter buckwheat seeds were germinated by light at 17.5 ℃ using a germination tester (Korea National Instruments, Korea) after soaking for 24 hours. After seed germination, germinated seeds were collected at intervals of 1 cm. The harvested seeds were dried at 40 ° C for 24 hours. Dried seeds were pulverized and used in the experiment.

3) Preparation of extract

1 g of the pulverized sample was taken, 100 mL of methanol was added, and the mixture was repeatedly extracted twice at 40 캜 for 2 hours. The extracts were concentrated by using a rotary vacuum concentrator (EYELA, N-1000, Tokyo, Japan) and lyophilized (EYELA, FDU-1200, Tokyo, Japan)

4) Measurement of DPPH free radical scavenging ability

Each extract was dissolved in methanol using methanol and then used. 1 mL of each extract was put into a test tube, 4 mL of 0.15 mM DPPH solution was added thereto, and the mixture was uniformly mixed. The mixture was allowed to stand at room temperature for 30 minutes and absorbance was measured at 517 nm. The DPPH free radical scavenging ability was expressed as a percentage of the absorbance difference between the sample added and the non-added sample.

DPPH free radical scavenging ability (%) = [1- (absorbance of sample addition sphere / absorbance of sample not added sphere) x 100]

5) Total polyphenol content measurement

The Folin-Denis method was slightly modified and measured. 0.2 mL of the extract was placed in a test tube, mixed with 1.8 mL of distilled water, mixed well, and left at room temperature for 3 minutes. 0.4 mL of a saturated solution of 10% Na ₂ CO ₃ was added and mixed, and 1.4 mL of distilled water was added to make a total of 4 mL. The mixture was allowed to stand at room temperature for 1 hour and absorbance was measured at 725 nm. Total polyphenol compounds were determined from standard curves prepared using gallic acid.

6) Tyrosinase activity

The inhibitory activity of tyrosinase, an enzyme affecting the production of melanin, was measured by measuring the absorbance of L-dopaquinone, a substance produced by reaction with L-DOPA, which is a substrate containing mushroom tyrosinase as an enzyme source. Add 0.2 mL of 0.1 M phosphate buffer (pH 6.8), 0.2 mL of 5 mM L-DOPA solution and 0.5 mL of sample, add 0.1 mL of tyrosinase (250 U / mL) and incubate at 35 ° C for 2 minutes. The absorbance at 475 nm Respectively.

7) HPLC analysis

The analytical sample was dissolved in HPLC water or methanol, filtered through a 0.45 μm membrane filter (Toyo Roshi Kaisha, Japan) and used for analysis. HPLC analysis conditions are as follows.

HPLC analysis conditions Condition Instrument Shimadzu LC system
(CBM-20A, Shimadzu Co, Ltd., Japan) column PrevailC18reversephase 150 x 4.6mm Mobile phase time A: 0.1% formic acid water B: 0.1% formic acid methanol 0 70 30 35 30 70 Oven temperature 40 ℃ Injection volume 10 μl Flow rate 1 ml / min. wavelength 355 nm

8) Statistical processing

Statistical significance was verified at the ρ <0.05 level by Duncan's multiple range test using SPSS (statistical package for social sciences, Version 10.0, Chicago, USA).

Example  One. DPPH  freedom Radical Scatters  Measurement result

However, the activity of both buckwheat and bitter buckwheat increased as the germination progressed, and then tended to decrease (Figs. 1A and 1B). In case of buckwheat, germinated 4 ~ 5cm germinated buckwheat showed the highest DPPH free radical scavenging ability in 5 ~ 6cm germinated experimental group. However, in case of buckwheat, the concentration of 1 ㎎ / mL of all buckwheat was higher than that of buckwheat (Fig. 1).

Example  2. Results of total polyphenol content measurement

In the case of buckwheat, the content of the experimental group germinated 4 to 5 cm was the highest at 91.93 mg / g, which was about 1.2 times that of the ungerminated experimental group (Fig. 2A). In the case of bitter buckwheat, the content of the germinated 5 ~ 6cm germinated group was the highest at 196.67mg / g and 1.2 times that of the germinated group (Fig. 2B). These results were similar to those of DPPH free radical scavenging. It is thought that the increase of phenolic compounds such as chlorogenic acid, tocopherol, etc. contained in buckwheat.

Example  3. Tyrosinase  Results of inhibitory activity measurement

In case of buckwheat, it was confirmed that the tyrosinase inhibitory activity was increased until germination of 4 to 5 cm (FIG. 3A). After 4 ~ 5 cm germinated experimental group, 44.17% activity was shown, and the inhibitory activity tended to decrease slowly again. The activity of the non - germinated experimental group was 76.49%, which was about 1.7 times higher than that of the experimental group showing the lowest tyrosinase activity.

The bitter buckwheat consistently inhibited the activity of tyrosinase until the experiment group germinated 5-6 cm, and then increased again (FIG. 3B). The tyrosinase activity in the 5 ~ 6cm germinated experimental group showed the highest inhibitory activity, which was 1.7 times higher than that of the non - germinated experimental group, which was 42.05%.

Example  4. HPLC  analysis

In the case of buckwheat, it was confirmed that orientin and isoorientin, which were not detected when not germinated, were detected at the beginning of germination (Figs. 4A and 4B). It was found that five flavonoid compounds, including rutin, increased steadily until germination progressed 4 ~ 5cm, and then decreased. When gentamicin was not germinated, the contents of 16.96 mg / mL, 5.49 mg / mL, and 5.44 mg / mL were found to be the highest in the germinated 4- , 126.67 mg / mL, and 118.53 mg / mL, which were about 7.1 times, 23 times, and 21.8 times, respectively. Orientin and isoorientin were not detectable in the non-germinated experimental group, but were found to be 187.56 mg / mL and 161.75 mg / mL in the experimental group germinated 4-5 cm after germination was started (Table 2).

In the case of bitter buckwheat, only the routine was detected in the ungerminated experimental group, but when the germination started, it was confirmed that four compounds including orientin were detected (FIGS. 5A and 5B). The bitter buckwheat increased steadily until germination progressed 5 ~ 6cm and then decreased. The rutin content of the germinated 5 ~ 6cm germinated group showed the highest value of 1334.67mg / mL, which was about 1.4 times higher than that of the germination group of 947.65mg / mL. Orientin, isoorientin, bitexin, and isovitectin did not appear when they did not germinate, but they steadily increased to 5 ~ 6 cm and then decreased. In the experimental group germinated 5 to 6 cm, the content of orientin, isoorientin, bitescicin and isovitectin was 6.94 mg / mL, 5.79 mg / mL, 14.83 mg / mL, and 19.64 mg / mL, respectively (Table 2).

Changes in the content of useful ingredients according to length of germination of buckwheat and bitter buckwheat Routine Orientin Isoorientin Beatty Isovichecin Dan Buckles 16.96 ± 0.18 a N.D. N.D. 5.49 + 0.03 a 5.44 + 0.03 a Soba less than 1cm 44.75 ± 0.47 b 41.78 ± 0.38 b 34.32 ± 0.32 b 30.02 ± 0.29 c 31.32 ± 0.30 c Soba 1 ~ 2cm 31.78 ± 0.30 b 21.99 + - 0.23 a 17.55 ± 0.20 a 19.09 ± 0.21 b 19.56 ± 0.21 b Sweet buckwheat 2 ~ 3cm 71.65 ± 0.57 c 66.41 + - 0.57 c 54.22 ± 0.48 c 50.67 ± 0.45 d 50.25 + - 0.45 d Soba 3 ~ 4cm 74.86 ± 0.57 d 147.85 ± 1.13 h 124.08 ± 1.13 i 104.83 + - 0.80 i 94.90 ± 0.73 h Dan Buckles  4 ~ 5cm 120.49 + - 0.92 g 187.56 ± 1.43 i 161.75 ± 1.24 j 126.67 ± 0.97 j 118.53 + - 0.91 i Soba 5 ~ 6cm 98.06 + - 0.75 f 141.31 + - 1.08 g 120.60 ± 0.92 h 94.44 + - 0.72 g 90.70 + - 0.69 g Dan Buckle 6 ~ 7cm 84.41 ± 0.65 138.00 ± 1.06 f 115.87 ± 0.89 f 104.60 ± 0.80 i 95.87 ± 0.73 h Dan Buckle 7 ~ 8cm 93.88 ± 0.78 fp 140.59 + - 1.07 g 119.20 ± 0.91 g 98.01 0.75 h 91.27 + - 0.70 g Dan Buckle 8 ~ 9cm 90.23 ± 0.84 116.85 ± 0.98 e 96.81 ± 0.83 e 85.66 ± 0.75 f 78.72 ± 0.69 f Safflower 9 ~ 10cm 84.18 + - 0.80 96.40 ± 0.83 d 79.33 ± 0.70 d 72.87 ± 0.65 e 70.29 ± 0.63 e Routine Orientin Isoorientin Beatty Isovichecin Bitter buckwheat 947.65 +/- 14.90 e N.D. N.D. N.D. N.D. Less than 1cm of buckwheat 734.46 + - 6.41 b 2.85 ± 0.02 c 1.98 + - 0.01 c 5.76 + 0.04 d 8.63 ± 0.08 d Bitter buckwheat 1 ~ 2cm 584.63 ± 10.59 a 1.43 + - 0.01 b 2.05 ± 0.02 d 3.95 + - 0.03 b 4.44 ± 0.03 b Bitter buckwheat 2 ~ 3cm 1116.83 + - 9.39 g 3.45 ± 0.03 d 2.89 ± 0.02 b 5.85 ± 0.05 c 8.28 ± 0.06 c 3 ~ 4cm bitter buckwheat 1120.86 +/- 8.57 h 4.48 ± 0.03 f 3.60 ± 0.03 g 11.39 ± 0.09 g 16.39 + - 0.13 h Bitter buckwheat 4 ~ 5cm 1307.20 ± 10.00 j 4.83 ± 0.04 i 3.66 ± 0.03 h 11.47 ± 0.09 g 16.61 + - 0.13 i Bitter buckwheat  5 ~ 6cm 1334.67 + - 10.21 k 6.94 + - 0.05 j 5.79 ± 0.04 j 14.83 + - 0.11 i 19.64 ± 0.15 j 6 ~ 7cm bitter buckwheat 1170.96 + - 9.80 i 4.38 ± 0.03 e 3.52 + 0.03 e 8.22 ± 0.06 e 11.96 ± 0.09 e 7 ~ 8cm bitter buckwheat 998.07 + - 8.74 f 4.81 ± 0.04 h 4.14 + 0.03 i 12.21 ± 0.09 h 16.25 + - 0.12 g 8 ~ 9cm bitter buckwheat 889.30 ± 7.81 d 4.69 ± 0.04 g 3.57 ± 0.03 f 10.76 ± 0.08 f 14.33 ± 0.11 f 9 ~ 10cm bitter buckwheat 756.71 + - 11.91 c 0.66 + 0.01 a 0.85 ± 0.01 a 1.41 + 0.01 a 2.04 0.02 a

Claims (11)

( Fagopyrum esculentum Moench) seeds germinated with a shoot length of 4 to 5 cm or a skin whitening agent containing methanol extracts of bitter buckwheat ( Fagopyrum tataricum Gaertner) seeds germinated with a length of 5 to 6 cm as an effective ingredient / RTI &gt; delete delete The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, Lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser Wherein the cosmetic composition has at least one of the following formulations. The cosmetic composition according to claim 1, wherein the cosmetic composition comprises at least one of a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a perfume, Or one or more adjuvants selected from nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, Wherein the skin-whitening cosmetic composition is a cosmetic composition for skin whitening. Fagopyrum esculentum Moench seeds with germ length of 4-5 cm or antioxidants containing methanol extract of bitter buckwheat ( Fagopyrum tataricum Gaertner) seeds germinated 5 to 6 cm in length as an effective ingredient Cosmetic composition. delete delete ( Fagopyrum esculentum Moench) seeds germinated with a shoot length of 4 to 5 cm or a methanol extract of a seed of a bitter buckwheat ( Fagopyrum tataricum Gaertner) germinated at a shoot length of 5 to 6 cm as an active ingredient A pharmaceutical composition for preventing or treating a deposition disorder. 10. The method according to claim 9, wherein the skin pigmentation disease occurs locally in the skin due to an increase in synthesis of melanin pigment and occurs in spots, freckles, black spots, nevi, drug-induced pigmentation, Wherein the pigment is at least one disease selected from hyperpigmentation, hyperpigmentation, hyperpigmentation, hyperpigmentation, and hyperpigmentation. ( Fagopyrum esculentum Moench) seeds germinated with a shoot length of 4 to 5 cm or a skin whitening agent containing methanol extracts of bitter buckwheat ( Fagopyrum tataricum Gaertner) seeds germinated with a length of 5 to 6 cm as an effective ingredient &Lt; / RTI &gt;

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