KR101440844B1 - Cosmetic composition with Linum usitatissimum seed mucus or the extract of Linum usitatissimum seed mucus - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a flaxseed mucus or a mucus extract, and is useful as a cosmetic composition for moisturizing the skin. In particular, it is characterized by excellent usability such as spreadability, spreadability, adhesion feeling and cushion feeling while reducing stickiness.

Description

[0001] The present invention relates to a cosmetic composition comprising a flaxseed mucilage or a mucus extract and a cosmetic composition comprising the extract of Linum usitatissimum seed mucus,

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition containing flaxseed mucus or mucus extract.

The skin is largely divided into three layers: epidermis, dermis, and subcutaneous tissue in order from the outside. It protects the internal organs of the body from changes in temperature and humidity, ultraviolet rays, and other physical and chemical external stimuli. Among them, the epidermis plays an important role in preventing water evaporation inside the human body.

The epidermis is divided into the stratum corneum, the granular layer, the superficial layer and the basal layer in order from the outside. Cells of the stratum corneum act as bricks, and the intercellular lipids between the keratinocytes function as mortar to form skin barrier J. Invest. Dermatol 80 (Suppl.), 44-49, 1983). In healthy human keratinocytes, a high concentration of Natural Moisturing Factor (NMF) is present to help maintain moisture in the skin. For example, substances such as amino acids are water-soluble and thus effectively combine with moisture to dry the skin (J. Invest. Dermatol. 54, 24-31, 1970).

On the other hand, industrialization due to the development of science has caused a drastic change in the global environment, which has had a serious impact on human health. As a result, various chronic and intractable diseases are rapidly increasing, and the incidence of environmental diseases such as atopic dermatitis, allergic rhinitis and asthma is increasing worldwide.

According to data released by the National Health Insurance Corporation's Health Insurance Research Institute during 2002-2007, the number of patients suffering from environmental diseases such as allergic rhinitis, atopic dermatitis and asthma was estimated to reach 552 million The number of health-related diseases increased by 29.3% from 7.14 million in 2007 to 3.25 million in 2003, and 3.9 million in 2003, .

In order to prevent the aforementioned environmental diseases, skin moisturization is indispensable. In the past, humectants having moisture-absorbing properties and Occlusive Moisturizers preventing evaporation of water have been used, and moisture content in the stratum corneum Has been performed. Examples of humectant include glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, 2-pyrrolidone-5-carboxylate and the like. This drawback has its drawbacks. (5), 731-740, 1994), there is a difficulty in maintaining the stability of the emulsified formulations, and there is a problem in that a transparent gel phase There is a disadvantage that it is not suitable for manufacturing a product.

However, recently it has been argued that the differentiation of the stratum corneum is important in preventing the drying of skin moisture. It has been found that the differentiation process developed from the keratinocytes of the basal layer to the keratinocytes of the outermost keratinocyte can function as a barrier to skin moisture retention. In other words, during the process of keratinization, the cells produce natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes rigid and supple, allowing it to function as a barrier. Physiological tendency of the skin to become dry as the age of the human is increased can be interpreted as a longer time for the removal of the stratum corneum, a lowering of the lipid synthesis ability of the epidermal cells, and a decrease in the amount of the moisturizing factor and lipid in the stratum corneum . Therefore, it is reasonable to propose a new approach to promote the differentiation of keratinocytes to induce strengthening of skin barrier, thereby enhancing skin moisture retaining function and protection from external environment.

On the other hand, flax is a one-year-old herb with a plant height of 70 ~ 110cm and a stem diameter of about 1 ~ 2mm. The flaxseed contains a lot of various nutrients, which can be a source of materials that can exert excellent functionality. However, its application is very limited for many reasons such as problems with digestion.

Flaxseed contains mucolytic components, and it has been a lot of effort to use flax as food for dieting, considering that this part can interfere with digestion or lower the absorbability of this part. Perhaps the description of mucus is well described in the Korean Registered Patent (10-1239432).

On the other hand, Korean Patent No. 10-1239432 has been widely used for separating and protecting flax-protecting mucus from other uses, but the research on the cosmetic composition using such mucolipids has been limited.

Since cosmetics are a preference product of consumers, it is an industrial field that combines science such as sensitivity, feeling, and courage such as sensibility and functionality. The feeling of use should be considered particularly important due to the nature of the cosmetic product in which various raw materials are combined. The main factors affecting the sensation of use are spreading, spreading, moisturizing, stickiness and cushioning.

Cosmetics researchers are continuously trying to increase the feeling of use, but it is not easy to make cosmetics with various ingredients mixed. There is a problem that the stickiness increases in almost all cases when the usability such as spreadability, spreadability, adhesion feeling, fogging property and cushion feeling is increased.

Therefore, development of a material capable of exhibiting excellent efficacy against spreadability, spreadability, cushioning, etc. without stickiness is required. In the present invention, such a material is developed and provided.

The present invention provides a cosmetic composition characterized by containing flaxseed mucus or mucus extract as an active ingredient. In the cosmetic composition of the present invention, the cosmetic composition may be, for example, for promoting the differentiation of dermal keratinocytes, for restoring skin barrier function, for skin moisturizing, and for improving feeling.

Meanwhile, in the cosmetic composition of the present invention, the flaxseed mucus or mucus extract may be contained in an amount of, for example, 0.001 to 100.0% by weight based on the total weight of the cosmetic composition.

In the cosmetic composition of the present invention, for example, the flaxseed mucus extract may be prepared by mixing (a) water, an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3- (B) a mucus extract obtained by adding ultrasonic waves to flaxseed extract, and (c) a mucus extract obtained by adding a supercritical solvent to flaxseed extract, wherein the extract is selected from the group consisting of .

Meanwhile, in the cosmetic composition of the present invention, the cosmetic composition may be an aqueous solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil, a powder foundation, , A wax foundation, and a spray.

Through the present invention, it was confirmed that flaxseed mucus or mucus extract promotes differentiation of dermal keratinocytes, restores skin barrier function, relieves skin pulling and roughness, and increases elasticity.

Therefore, the flaxseed mucus or extract of the present invention can be used as a cosmetic composition for skin moisturizing, and can be effectively used as a cosmetic composition for skin moisturizing. Particularly, it has excellent properties such as spreadability, spreadability, have.

The flaxseed mucilage or mucus extract contained in the cosmetic composition of the present invention contains a pectin-like substance of a heretofore unknown composition. The role of this substance is to protect the passing seeds through the digestive tract of an animal, It can keep survival. Human digestive tracts can not digest untreated, untreated flaxseeds similar to animals.

One kilogram of flaxseed can combine 5 liters of water, resulting in the gastric juice being unable to reach the surface of the seed coat. This slippery gel-like material significantly improves bowel activity and, in many cases, (purgative substance). In addition, flaxseed mucilage is known to contain many substances that interfere with digestion, such as hydrocyanids and trypsin inhibitors. Although these effects can be reduced by heat treatment, there is a report that their interference effects can not be completely eliminated (Journal of the American Oil Chemists' Society 70 (9): 899-904, 1993).

In the present invention, the use of flaxseed mucilage or mucus extract having the above-mentioned characteristics is intended to provide an effective ingredient of a cosmetic composition after confirming its usefulness as a cosmetic composition. The flaxseed mucus of the present invention may be obtained by a physical desorption method such as scraping from flaxseed and may be carried out by a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure, May also be obtained in the form of an extract.

For example, the flaxseed mucus extract of the present invention may be at least one selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butyleneglycol, Can be extracted using an extraction solvent, preferably extracted using ethanol, 70% ethanol or water, more preferably 70% ethanol. It is obvious to those skilled in the art that the extract of flaxseed mucus of the present invention can be obtained not only by the above-mentioned extraction solvent but also by using other extraction solvent, which exhibits substantially the same effect.

In addition to the general extraction method described above, supercritical extraction using a supercritical extraction solvent or ultrasonic extraction using ultrasonic waves may be used to produce a flaxseed mucus extract from flaxseed. Since the detailed processes of the supercritical extraction method and the ultrasonic extraction method are already well known, detailed description thereof will be omitted.

On the other hand, the flaxseed mucus extract of the present invention includes not only the extract obtained by the above-mentioned extraction solvent, but also the extract obtained through a usual purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The active fraction is also included in the extract of the present invention.

In addition, the flaxseed mucus extract of the present invention may be a liquid obtained by cooling or heating the flaxseed mucus extract at room temperature, followed by filtration, and may be obtained by concentration under reduced pressure or lyophilization.

Meanwhile, in the present invention, the flaxseed mucus may be prepared by fermentation and cultivation with the following enzyme solution. As the enzyme solution, an enzyme solution to which a pectin-degrading enzyme or a fibrinolytic enzyme is added may be used, and optionally, a proteolytic enzyme may be contained. Examples of the pectin-degrading enzyme include pectinase (Macerozyme R-10 and the like), cellulolytic enzyme such as cellulase (Onozuka R-10 and the like), protease as protease and the like. The enzyme is added to the flaxseed usually at a ratio of 1 to several times, preferably 2 times, the volume of the magnetic volume, and the enzyme is added thereto at 20 to 40 캜, preferably 25 to 37 캜, for 2 to 24 hours, It can be cultured for 12 hours.

Meanwhile, in the present invention, the separation or filtration of flaxseed mucilage or mucus extract can be carried out by using a conventional filtration device such as a Wattmann filter paper, a filtration device with 100 to 400 mesh or the like, a vacuum filtration device, Stirring and rotation by gravity) can be used.

On the other hand, the flaxseed mucus or mucus extract of the present invention may be contained in an amount of 0.001 to 100.0% by weight, preferably 0.1 to 60.0% by weight, and most preferably 0.1 to 30.0% by weight, . When the content of flaxseed mucilage or mucus extract is less than 0.001% by weight, the skin improvement effect is not exhibited.

According to the present invention, the flaxseed mucilage or mucus extract of the present invention is excellent in skin keratinocyte differentiation promoting effect, skin barrier function restoration effect, moisturizing effect and feeling improving effect. The cosmetic composition of the present invention is an effective ingredient, In addition to the mucus extract, it may contain the components conventionally used in cosmetic compositions, and may include customary adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation, and spray, but the present invention is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane, such as butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

On the other hand, the present invention also provides a cosmetic method characterized by applying a cosmetic composition containing the extract of flaxseed mucus of the present invention to human skin. The cosmetic process of the present invention includes all cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or it may be used after being overlapped with other cosmetic compositions other than the present invention. In addition, the cosmetic composition of the present invention can be used according to a conventional method of use, and the number of times of use may be varied depending on the skin condition or taste of the user. When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , A cleansing lotion, a cleansing water or a cleansing gel, but is not limited thereto.

When the cosmetic composition comprising the extract of flaxseed mucus of the present invention is applied to human skin, it is possible to obtain a skin keratinocyte differentiation promoting effect, a skin barrier function restoration effect, and a skin moisturizing effect.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Manufacturing example  1: Preparation of flaxseed mucus extract]

10.0 g of flaxseed was put into 500 L of purified water, stirred at 25 DEG C for 5 hours, and then filtered through a 300-mesh filter cloth. Thereafter, an equal amount of alcohol was added to the filtrate to precipitate. 5, and then dried to obtain about 0.45 g of a white powder.

[ Manufacturing example  2: Preparation of mucilage mucus extract]

10.0 g of flaxseed were placed in 500 L of purified water, stirred at 25 캜 for 5 hours, and then filtered with a 300-mesh filter cloth. Thereafter, the filtrate was lyophilized under the conditions of -48 캜 and 0.1 toor to obtain about 0.5 g of a white powder.

[ Manufacturing example  3: Preparation of flaxseed mucus extract]

After adding 500 g of purified water to 10.0 g of flaxseed, 100 ng of pectinase (Macerozyme R-10) and 10 g of cellulase (Onozuka R-10) as a fibrinolytic enzyme were added, followed by incubation at 37 ° C for 12 hours. Then, the mucous layer was filtered with 300 mesh filter cloth. Thereafter, the filtrate was lyophilized under the conditions of -48 캜 and 0.1 toor to obtain about 0.5 g of a white powder.

[ Manufacturing example  4: Preparation of mucilage mucus extract]

10 g of flaxseed was put into 1.2 L of water and extracted at 65 DEG C for 5 hours. Then, the mixture was filtered through a 300-mesh filter cloth, allowed to stand at 4 DEG C for 5 days, aged, and then filtered through Wattman filter paper No. 5. The filtrate was dried at 65 ° C with a rotary attenuator to obtain 0.83 g of dry weight.

[ Manufacturing example  5 ~ 22: Preparation of flaxseed mucus extract]

The flaxseed extract of Preparation Examples 5 to 22 was obtained by extracting flaxseed in the same manner as in Production Example 4, except that the extraction solvent of Table 1 was used in place of water.

extract Extraction solvent Dry weight of final extract
(Unit: g) Production Example 5 10% ethanol 0.8 Production Example 6 20% ethanol 0.9 Production Example 7 30% ethanol 0.2 Production Example 8 40% ethanol 0.3 Production Example 9 50% ethanol 0.1 Production Example 10 60% ethanol 0.3 Production Example 11 70% ethanol 0.2 Production Example 12 80% ethanol 0.3 Production Example 13 90% ethanol 0.5 Production Example 14 100% ethanol 0.4 Production Example 15 Methanol 0.3 Production Example 16 n-propanol 0.5 Production Example 17 Isopropanol 0.6 Production Example 18 2-butanol 0.2 Production Example 19 Acetone 0.5 Production example 20 chloroform 0.5 Production Example 21 Ethyl acetate 0.3 Production Example 22 Butyl acetate 0.6

[Preparation Example 23: Preparation of flaxseed slurry]

The sclera was used to scrape mucus from flaxseed to obtain 10 g of flaxseed mucilage.

[ Experimental Example  1: promoting effect of keratinocyte differentiation]

Human keratinocytes were cultured in a culture flask and grown to about 80%. The flaxseed mucus extract (Preparation Example 2) was added at concentrations of 10, 50, 100, and 200 ppm, respectively, Then, the protein was dissolved by mixing a urea, a denaturant such as sodium dodecyl sulfate (SDS), and a reducing agent such as? -Mercaptoethanol. After that, the remaining horny layer was measured for absorbance at 310 nm to analyze the peptide concentration, and the amount of increase by each sample was compared with that of distilled water as a negative control (0%). At this time, TGF-β 10 nM was treated as a positive control.

Concentration (ppm) Promoting effect of keratinocyte differentiation (%) 10 40.12 50 65.72 100 81.45 200 155.24 Negative control (distilled water) 0 Positive control (TGF-β) 10 nM 145.25

As can be seen from the results of Table 1, it was confirmed that the flaxseed mucus extract used in the present invention has an excellent effect of promoting keratinocyte differentiation.

[ Prescription example  1: softening longevity]

As an example of the cosmetic material, the softening longevity containing the flaxseed extract of Preparation Example 2 was prescribed, and the composition was as shown in Table 3 below.

ingredient Content (unit:% by weight) Flaxseed mucus extract
glycerin
1,3-butylene glycol
PIZZY 1500
Allantoin
DL-Panthenol
Lee D. TEE-2NA
Benzophenone-9
Sodium hyaruronate
ethanol
Octidodeces-16
Polysorbate 20
Preservative, fragrance, pigment
Distilled water 10.0
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.2
a very small amount
Balance Sum 100

[ Prescription example  2: convergent lotion]

As an example of the cosmetic material, a convergent lotion containing the extract of flaxseed mucilage of Preparation Example 2 was prescribed. The prescription examples are shown in Table 4 below.

ingredient Content (unit:% by weight) Flaxseed mucus extract
glycerin
1,3-butylene glycol
Allantoin
DL-Panthenol
EDTA-2Na
Benzophenone-9
Sodium hyaruronate
ethanol
Polysorbate 20
Location of Hazel extract
Citric acid
Preservative, fragrance, pigment
Distilled water 30.0
2.0
2.0
0.2
0.2
0.02
0.04
3.0
15.0
0.3
2.0
a very small amount
a very small amount
Balance Sum 100

[ Prescription example  3: Nourishing lotion]

As an example of the cosmetic, a nutritional lotion containing the extract of flaxseed mucilage of Preparation Example 2 was prescribed. The prescription examples were as shown in Table 5 below.

ingredient Content (unit:% by weight) Flaxseed mucus extract
Glyceryl stearate SE
Stearyl alcohol
lanolin
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Mineral oil
Squalane
Trioctanoin
Dimethicone
Tocopheryl acetate
Carboxyvinyl polymer
glycerin
1,3-butylene glycol
Sodium hyaruronate
Triethanolamine
Preservative, fragrance, pigment
Distilled water 50.0
1.5
1.5
1.5
1.3
0.5
1.0
5.0
3.0
2.0
0.8
0.5
0.12
5.0
3.0
5.0
0.12
a very small amount
Balance Sum 100

[ Prescription example  4: nutrition cream]

As an example of the cosmetic material, a nutritional cream containing the extract of flaxseed mucilage of Preparation Example 2 was used, and the prescription examples were as shown in Table 6 below.

Component (content:% by weight) Example 1 Comparative Example 1 Comparative Example 2 Flaxseed mucus extract
1,3-butylene glycol
Pro-type glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaruronate
Preservative, fragrance, pigment
Distilled water 5.0
-
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance -
-
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance -
5.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance Sum 100 100

[ Prescription example  5: Massage  cream]

As an example of a cosmetic preparation, a massage cream containing the extract of flaxseed mucilage of Preparation Example 2 was used. The prescription examples were as shown in Table 7 below.

ingredient Content (unit:% by weight) Flaxseed mucus extract
Pro-type glycerin monostearate
Stearyl alcohol
Stearic acid
Polysorbate 60
Sorbitan stearate
Isostearyl isoserate
Squalane
Mineral oil
Dimethicone
Hydroxyethyl cellulose
glycerin
Triethanolamine
Preservative, fragrance, pigment
Distilled water 45.0
1.5
1.5
1.0
1.5
0.6
5.0
5.0
35.0
0.5
0.12
6.0
0.7
a very small amount
Balance Sum 100

[ Prescription example  6: Essence]

As an example of the cosmetic material, an essence containing the extract of flaxseed mucilage of Preparation Example 2 was prescribed, and the prescription examples were as shown in Table 8 below.

ingredient Content (unit:% by weight) Flaxseed mucus extract
glycerin
Betaine
PIZZY 1500
Allantoin
DL-Panthenol
Lee D. T.-2Na
Benzophenone-9
Hydroxyethyl cellulose
Sodium hyaruronate
Carboxyvinyl polymer
Triethanolamine
Octyldodecanol
Octyldodec-16
ethanol
Preservative, fragrance, pigment
Distilled water 70.0
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
Balance Sum 100

[ Prescription example  7: Pack]

As an example of the cosmetic, a pack containing the flaxseed mucilage extract of Production Example 2 was prescribed, and the prescription examples were as shown in Table 9 below.

ingredient Content (unit:% by weight) Flaxseed mucus extract
Polyvinyl alcohol
Cellulose sword
glycerin
PIZZY 1500
Cyclostristin
DL - Panthenol
Allantoin
Monoammonium glycyrrhizin acid
Nicotinamide
ethanol
FYGEN 40 Hardened castor oil
Fragrance, coloring, preservative
Distilled water 10.0
15.0
0.15
3.0
2.0
0.15
0.4
0.1
0.3
0.5
6.0
0.3
a very small amount
Balance Sum 100

[ Experimental Example  2: Skin barrier function recovery effect]

In order to examine the skin barrier function recovery effect of cosmetics containing the extract of flaxseed mucus of the present invention, the following experiment was conducted.

Experiments were carried out by using a taping stripping method to damage the skin barrier and measuring the skin water loss (TEWL, transdermal water loss) to 4.0 g / m 2 / h measured by servomed (Sweden) evaporimeter EP 1 , The nutritional creams of Example 1 and Comparative Examples 1 and 2 containing the flaxseed mucilage extract of the present invention prepared in the aforementioned Prescription Example 4 were applied to an area of 5 cm 2 and the skin moisture loss was measured for 1 hour and 2 hours , 4 hours, and 8 hours after the administration, and evaluated for the extent to which the barrier function was restored. At this time, a known lipid mixture (ceramide: 2: 1: 1 mixture of cholesterol: fatty acid) was used as a positive control. The results are shown in Table 10 below, with changes in moisture content of the skin in the initial damaged barrier state being taken as a reference (100%) and compared with time.

sample 0 hours 1 hours 2 hours 4 hours 8 hours Example 1 85 63 25 20 20 Comparative Example 1 85 70 40 30 21 Comparative Example 2 85 65 43 32 23 Control group 85 65 47 35 25

As shown in the results of Table 10, it was confirmed that the skin barrier function recovery effect of Example 1, which is a cosmetic containing the extract of flaxseed mucilage of the present invention, is superior to those of Comparative Examples 1 and 2.

[ Experimental Example  3: Skin Moisture power  Improvement effect]

In order to examine the skin moisturizing effect of the cosmetic composition containing the extract of flaxseed mucus of the present invention, the following experiment was conducted. Twenty-five to twenty-four patients without skin disease were divided into two groups of twenty persons per one group. In each group, Example 1 and Comparative Examples 1 to 2, which contained the flaxseed mucus extract prepared in the above-mentioned Prescription Example 4 Of the nutritional cream was applied to the face and forehead twice a day for one month.

The skin conductivity was measured in advance using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application and the values were measured at 1 week, 2 weeks, The skin conductivity increase rate (%) was measured and evaluated. The results are shown in Table 11 below.

sample After 1 week after 2 weeks After 4 weeks Example 1 57 63 65 Comparative Example 1 27 35 42 Comparative Example 2 31 39 48

As shown in the results of Table 11, in Example 1, which is a cosmetic containing the extract of flaxseed mushroom of the present invention, the rate of skin conductivity increase was much higher than those of Comparative Examples 1 and 2. In general, the skin conductivity is proportional to the skin moisture content. From the above results, it can be seen that the cosmetic composition containing the extract of flaxseed extract in the present invention keeps the skin moisture content higher than that of the non-cosmetic ingredients.

Therefore, it was found that the cosmetic composition containing the extract of flaxseed mucus of the present invention is excellent in improving the skin moisturizing ability.

[ Experimental Example  4: Feeling  Evaluation of improvement effect]

Evaluation of feeling improvement effect was carried out as follows. Twenty women between the ages of 30 and 40 were randomly divided into two groups, and the nutritional creams of Example 1 and Comparative Example 2 were washed twice daily in the morning and evening, and then an appropriate amount was continuously and centered around the eyes. After that, the feeling of each subject was evaluated through a user questionnaire. 1 to 10 points were given depending on the characteristics of the parameters to be measured (wetting, stickiness, cushioning, anesthesia, and adhesion). At this time, if the characteristic is low, one point is given to the one point and the high point is given to the ten point.

The experimental results are shown in Table 12 below. Each evaluation item was rated on a scale of 10, and the results as shown in Table 12 were obtained.

sample Brittle Stickiness Cushion feeling Yu Chen Adhesiveness Example 1 9.3 2.0 9.8 9.6 9.7 Comparative Example 2 5.5 5.2 6.4 6.7 7.1

As can be seen from the results of Table 12, the cream of the present invention containing the extract of flaxseed mushroom extract, as compared with Comparative Example 2 using 1,3-butylene glycol, which is widely used as a moisturizer and a feeling- It was found that the feeling of feeling of using cosmetics was excellent, and the stickiness was very small.

Therefore, it was confirmed that the extract of the present invention of flaxseed mushroom has an excellent effect as a feeling-of-use improving agent for cosmetics.

[ Experimental Example  5: Survey]

In order to examine the skin moisturizing effect of the cosmetic composition containing the extract of flaxseed mucilage of the present invention, the following experiment was conducted. The nutritional cream of Example 1 and Comparative Example 1 containing the flaxseed mucilage extract prepared in Prescription Example 4 were divided into two groups of 20 persons per one group, 2 nutritional creams were applied to the face and forehead twice a day for one month.

Subjective efficacy was evaluated by questionnaire survey on skin pull, roughness, and elasticity change after using Example 1 and Comparative Examples 1 and 2 for the test subjects. The questionnaire was divided into 1 ~ 5 points (satisfaction with the higher number is satisfied) about the effect of the product on each item of skin pull, roughness, and elasticity change, and the results are shown in Table 13 below.

sample
Satisfaction (average score) Better skin tightening Skin roughness improvement Increase skin elasticity Example 1 4.3 4.8 4.8 Comparative Example 1 3.7 3.3 3.0 Comparative Example 2 3.5 3.5 3.2

As shown in the results of Table 13, in the case of Example 1, which is a cosmetic containing the extract of flaxseed mushroom of the present invention of the present invention, the effect of improving the pulling, roughness and gloss of the skin is superior to those of Comparative Examples 1 and 2 .

Claims (8)

Flaxseed mucilage or mucus extract as an active ingredient,
A cosmetic composition for improving feeling, skin moisturizing, promoting differentiation of skin keratinocyte, and restoring skin barrier function.
delete delete delete delete The method according to claim 1,
The flaxseed mucilage or mucus extract,
Is contained in an amount of 0.001 to 100.0% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
The above-mentioned flaxseed mucus extract,
(a) a mucilage extract obtained by extracting water with an extracting solvent selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol; (b) a mucus extract obtained by adding an ultrasonic wave to flaxseed extract, and (c) a mucus extract obtained by adding a supercritical solvent to flaxseed extract.
The method according to claim 1,
In the cosmetic composition,
Is any one selected from the group consisting of aqueous solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations and sprays ≪ / RTI >