KR101420211B1 - Skin Composition for External Application Using Bud and Sprout Extract - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing a bud or an extract of a plant, and more particularly, to a composition for external application for skin comprising bud extract, broccoli, oilseed rape, sprout, safflower, clove and the like, As an active ingredient, to a composition for external application for skin.
In addition, the composition for external application for skin according to the present invention further includes aloe leaf extract, Centella asiatica extract, and allium extract.

Description

[0001] The present invention relates to a skin composition for external application using Bud extract and sprout extract,

The present invention relates to a composition for external application for skin containing a bud or an extract of a plant, and more particularly, to a composition for external application for skin comprising bud extract, broccoli, oilseed rape, sprout, safflower, clove and the like, As an active ingredient, to a composition for external application for skin.

Skin is an organs covering the entire surface of the body. It protects the human body from the external environment and stimulation, and suppresses evaporation of water in the body. Therefore, the skin is exposed directly to the external environment.

The activity of the cells constituting the skin and the skin protecting ability against the external infectious diseases are decreased as the ozone layer destruction increases the ultraviolet rays reaching the surface, weakening the immunity due to stress, and exposure to pollution sources such as toxic chemicals. As a result, the skin is sensitive to external stimuli, and skin troubles such as erythema, edema, itching, and inflammatory reaction are induced. In addition, the substances produced during the inflammatory reaction cause skin pigmentation, skin elastic fibers and collagen fibers to collapse, thus affecting the increase of skin wrinkles.

A significant amount of reactive oxygen species (ROS) are generated during the oxidation process for energy production in vivo. Most of these reactive oxygen species disappear by the in vivo eliminator, but when large amounts of active oxygen species are instantly generated or chronic active oxygen species are generated and the balance with the antioxidant defense system is broken, it becomes a cause of various diseases.

Active oxygen species include oxygen (oxygen, ¹ O _2), hydrogen peroxide (superoxide, O ₂ ^-) There are, such as the hydroxyl radical (hydroxyl radical, OH), hydrogen peroxide _{(hydrogen peroxide, H 2 O 2} ), which skin cells and tissues , As well as destroying the balance of antioxidants composed of antioxidant enzymes and non-enzymatic antioxidants and destroying the balance of oxidants and antioxidants and promoting the production of lipid peroxidation, protein oxidation, chain cleavage of collagen and elastin and melanogenesis reaction, Lt; / RTI >

As a result, skin aging of skin such as reduced elasticity, wrinkles, spots and freckles is accelerated. Therefore, in order to delay and inhibit skin aging, it is necessary to develop an antioxidant capable of inhibiting excess active oxygen species in the skin as well as in vivo and efficiently removing active oxygen species.

Inflammation is one of physiological defense reactions of biological tissues to external stimuli. Clinically, symptoms such as fever, fever, swelling, pain, and dysfunction are observed. This is caused by intrusion of external foreign substances such as bacteria and mechanical damage To protect the skin from the physiological mechanism.

Various inflammatory mediators activate oxidant production and reduce tissue antioxidant capacity, thus impairing the basic metabolic structural factors of the tissue and causing toxicity. Oxidation in these various cells and tissues produces a unique oxidant, A secondary tissue reaction of reactive oxygen species begins.

Active oxygen species and reactive nitrogen species (RNS) are associated with inflammatory responses that regulate the action of various cells in vivo. In the case of inflammation, lipid peroxidation of the cell membrane by the active oxygen species and degradation of the protein can not maintain the cell membrane structure, resulting in a change in the calcium ion channel of the cell membrane. These changes lead to the activation of phospholipase A ₂ (PLA ₂ ) as a calcium ion catalyst as the intracellular Ca 2+ influx increases.

The active oxygen species activates PLA2 in mast cells, and the arachidonic acid produced by it produces the inflammatory mediators prostaglandins and leukotrienes by the action of cyclooxygenase (COX) and lipoxygenase.

Therefore, the inhibitors of lipoxygenase and cyclooxygenase not only inhibit the production of inflammatory mediators but also inhibit cell membrane destruction and peroxidation by reactive oxygen species produced during inflammation, and may exhibit anti-inflammatory action. It also inhibits proteins and lipolytic enzymes that are produced by hyperinflammation, thereby protecting cells that are damaged thereby to inhibit skin aging.

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1. Korean Patent Publication No. 10-2006-0025630 (Published date: March 22, 2006) 2. Korean Unexamined Patent Publication No. 10-2011-0092971 (Published Date: Aug. 18, 2011)

The inventors of the present application have continued to carry out in-depth research and various experiments, and have found that the active ingredient of the present invention is an extract of sprouts, broccoli, rapeseed, sprouts, safflower, clove and flower buds, sprout buds extracted from tea leaves, The present inventors have found that the skin composition of the present invention can inhibit or ameliorate skin aging by promoting skin regeneration and proliferation, and have completed the present invention.

The present invention has the following features in order to achieve the above object.

The composition for external application for skin according to one embodiment of the present invention is characterized by comprising, as an active ingredient, a sprout extract comprising a bud sprout extract, a broccoli sprout extract, a rape bud sprout extract, a budrose sprout extract, a safflower sprout extract and a clover sprout extract, Extract, Seedlings of Seedlings and Seedlings of Tea Leaves.

Wherein the sprout extract is selected from the group consisting of 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 To 5% by weight of the extract. The extract contains the larvae of the flower buds, the larvae of the larvae and the extract of the tea leaf larvae at a weight ratio of 1: 5: 1 to 5: 1 to 5.

The composition for external application for skin according to the present invention contains the sprout extract and the sprout extract at a weight ratio of 1: 2: 1 to 2, and the total weight of the sprout extract and the sprout extract is 0.001 to 10% by weight based on the total weight of the composition.

The extracts of the buds and the extracts of the sprouts are prepared by hot extraction, filtration and concentration under reduced pressure of hydrothermal, C _{1 -4} lower alcohol or a mixed solvent thereof, and the composition for external application for skin is selected from the group consisting of softening longevity, Cream, pack, gel or skin adhesive type cosmetic, or a lotion, ointment, gel, cream, patch or spray.

The composition for external use on the skin may further contain at least one selected from the group consisting of aloe leaf extract, centilla sp.

The composition of the present invention comprising a bud extract and a sprout extract according to the present invention has an effect of enhancing the activity of skin epidermal stem cells and promoting the proliferation of skin cells as well as enhancing the antioxidant ability, It is effective.

FIG. 1 is a graph showing that a mixture of 6 sprout extracts and 3 sprout extracts in a photoperiod animal model using hairless mice inhibited collagen degradation by ultraviolet rays to increase the amount of collagen in skin.

Therefore, the composition according to the present invention can be used as an extract of buds, a bud sprout extract, a broccoli sprout extract, a rape bud sprout extract, a bud sprout extract, a safflower sprout extract and a clover sprout extract, And tea leaf extract. It contains as an active ingredient.

Generally, a bud is a stem cell of a plant. Unlike animals, plants have the potential to make whole plants become stem cells. This is because the plant has only the tissue and is not differentiated into the organ. A representative adult stem cell of a plant is a cell in the growth point of a root or stem end, and a seed etc. corresponds to an embryonic stem cell of an animal. Therefore, stem, stem or leaf part can be a constantly dividing stem cell under certain condition, and buds in particular contain abundant variety of substances necessary for growth.

Accordingly, the present invention provides a composition containing a sprout extract having an excellent effect of activating skin epidermal stem cells and promoting the growth of skin cells as well as increasing the expression of various growth factors affecting skin regeneration and skin aging delay. to provide.

The six sprouts and three sprouts contained as an active ingredient in the composition according to the present invention will now be described in detail.

Firstly, wheat (Triticum vulgare) is a year-old seedling of the almond leaf plant lanceolate. It grows about 1m in height and 2 ~ 3 generations together. It is plain and has long nodes. Flowers bloom in May, fruit matures in June and July, and the spirit and crown are wide ellipsoid, brown and fall well in the ying. The fruit ingredient contains 53 ~ 70% of starch, 11 ~ 11% of protein, 2 ~ 7% of sugar (sucrose and glucose), 2 ~ 10% of texturin, 1.6% of oil and it contains citrate, lecithin and allantoin. It contains tocopherol to prevent skin aging and make skin elastic. It is also used for sweating, osteoporosis, and digestion.

In the present invention, wheat, a bud of wheat, is used. Sprouts are germinated for about 5 to 10 days. Sprouts are germinated and synthesize new materials as weapons to defend themselves from the harmful environment outside. They contain useful substances such as various enzymes, vitamins and amino acids that were not in the seed state.

Germinated broccoli ( Brassica oleracea there is . italica ) contains a large amount of anti-cancer substances called sulfolafan and is known to be effective in preventing cancer and suppressing cancer cells. In addition, it contains beta carotene, vitamin B, C and so on.

The rapeseed (Brassica campestris subsp. Napus var. Nippo-oleifera) used in the present invention is a dicotyledonous plant and is a biennial plant of a poppy bark. The rapeseed is also called plains, and is usually of the species Brassica napus and B. campestris.

The common species is native to the Central Asian highlands from the Mediterranean coast, flowers are yellow and seeds are reddish brown, so they are also called red species. The Western species originate in the Scandinavian peninsula from Siberia to the Caucasus region, It is greenish red and the seed is blackish brown. It is a western species cultivated in Korea. Since 1962, it has been cultivated as a paying crop (oil product) in earnest. The rape has a height of 80 to 130 cm, and the surface is smooth and green. In the main stem, there are about 15 primary branches and two to four secondary branch branches. Leaves are basal and end is dull. Leaves on lower stem have long petiole. Leaf edge is deeply cracked. Leaf on upper stem has no petiole, it surrounds stem and its tip is thin.

Leaves of western varieties are thick and swollen (leather: leather-like texture), and the species is light green and the leaves are undrained. The stem usually has 30 to 50 leaves. The flowers bloom in April ~ April, bloom on the end of the branches, similar to the Chinese cabbage, and one flower with a peduncle about 10 cm long. Petal and calyx are divided into 4 pieces each, and there are 6 stamens, 1 pistil and 4 honey glands. The length of mature ears varies depending on the location of the branches, the method of planting, the variety, etc., but it is usually 35 to 45 ㎝, with 30 to 40 fruits per ears. The fruit is angular and has a cylindrical shape with a length of about 8 ㎝. In the center there is a suture line, and when ripe, the suture line is split and the seed falls.

The genus is divided into 2 chambers and is divided into a transparent diaphragm, usually containing about 20 dark brown seeds. Breeding is a seed, and there is a difference in the degree of seeding in varieties. In contrast, in western varieties cultivated in Korea, the sensitivity to light is weak and the degree of variability depends on the difference in temperature sensitivity. It is a good variety with high yield, good quality, fast growing and good resistance to cold and moisture. Representative varieties are Asahi, Yudal, Mokpo 11 and Yongdang. Seeds contain 38 to 45% of oil, 15 to 20% of soluble nitrogen and 20% of the protein contains cooking oil as the next most consumption of soybean oil is used as feed or fertilizer.

The flower is a herbaceous plant, and the fatty oil separated from the seed is used as a lubricant for the ointment base, oily injection oil, and machine, and is used in Korea (Jeju and South), Japan , And China.

In addition, the bud refers to the germination of soybeans. When the soybeans are germinated in sunshine instead of in the shade, photosynthesis can produce beans with nutrient additions that are not present in the soybeans.

The bean sprouts produced in sunny area are smaller than bean sprouts germinated in shade, the leaves are silky, the stems are stiff and stiff, and are not suitable for edible use.

In the present invention, sufficient moisture was supplied from the sunny place and the soybean was germinated. As a result, the beans could be obtained in only one day. However, since the size was small and the nutrients were not sufficiently replenished, the beans containing the proper size and nutrients were obtained As a result of selecting the period of germination, it is preferable to germinate at a period of about 5 to 7 days.

The reason for this is that when germinated for less than 5 days, the content of nutrients is remarkably low, and when germinated for more than 7 days, the fiber contained in the sprouts is not suitable for the desired extract.

Further, in the present invention, hot water, C _{1 -4} lower alcohol or a mixed solvent thereof is extracted with warming, filtered, and concentrated under reduced pressure to obtain an extract.

In addition, safflower (Carthamus tinctorius L.) is also known as safflower and is a one-year-old herb belonging to the Asteraceae family (Compoitae), and has been used as a diuretic for treating bone diseases such as osteoporosis, osteoporosis, .

As a result of medically proving the therapeutic effect of the sickle bones disease, the production of safflower has greatly increased recently. Recent research has shown that safflower contains serotonin compounds with excellent antioxidant activity, acacetin as a flavonoid ingredient and lignan compounds as effective against bone diseases. It has been reported that the powder not only improves the therapeutic effect of bone diseases but also improves lipid metabolism.

Clove is also known as Eugenia caryophyliate, whose main constituent is linalylacetate, Linalool and Eugenol, and has anti-convulsant, antidepressant and strong nervous system Soothing effects and so on.

In addition, Sophora japonica L. is a broad-leaved arboreous tree belonging to legume plants and widely distributed in Korea, China, and Japan. Painted trees have gray or dark gray barks, and yellowish-green blooms have a peculiar smell. Painted tree flowers bloom mainly in July-August, and the flowers of this period are called 槐花 (槐花) in one shot, and the buds are called 미米 (槐米).

In addition, it is used for hemostasis, hematemesis, blood flow, and is known to have hypotensive effect and anti-inflammatory effect. In the private sector, boiled water is used to wash the hemorrhoids, and raw or fried calves are used for nasal bleeding, bacterial hemorrhoid healing, hypertensive patients to prevent paralysis (Korea Natural Medicine Research Association, 1985).

It has been reported that isoflavones and their glycosides Biochain A-7-D-gentiobioside, sissotrin and pratensein have been isolated from the tree trunk (Takeda et al., 1977).

It is a naturalized plant that came in from China. Many shrubs are planted for ornamental purposes. It reaches 15m in height and branches are split a lot. Leaves are opposite to each other and look like inverted eggs with flat edges. There are hairs on both sides but gradually disappear, and petiole length is 7 ~ 15mm.

Flowers bloom earlier than leaves in April, and are black purple. Calyx is green and has three pieces. It has 6 petals, 10cm long and it blooms when it is fully sunlight. The petals are dark purple on the outside and light purple on the inside. There are many operations and pistils.

The fruit is egg-shaped oval, and it turns into a lot of corolla. It ripens brown in October, and the red seed hangs on the thread. M. denudata var. Purpurascens is often referred to as petals whose surface is light purplish reddish purple and white inside.

Finally, the tea tree is an evergreen small arboreous tree belonging to the tea family. It grows about 10m tall but grows easily. It usually grows about 1m and there are many branches.

The elliptical leaves have dull sawtooth on the edge. Both ends and base are pointed, and the flowers bloom in white or light pink, 1 ~ 3 on the leaf axil or end of the branch from October to November. The fruit is round and has a round shape.

The above-mentioned tea leaves are used as dried green tea or green tea by depressing the action of the enzyme by stinging or heating the young leaves.

The leaves of the tea tree (hereinafter referred to as green tea leaves) are widely used as a precious ingredient all over the world because they contain caffeine, tannin, catechin, vitamins, amino acids, nitrogen compounds, chlorophyll, carbohydrates, organic acids, Caffeine contained in green tea has a detoxifying effect because it acts on precipitation of toxic substances such as heavy metals and alkaloids and proteins by tannins and acts on the metabolism by vitamins And plays a role in maintaining normal functions such as heart, blood vessel, and stomach.

In addition, fluoride contained in tea plants protect teeth from acid by coating the surface of teeth, inhibits development of tooth decay bacteria causing tooth decay, inhibits the activity of GTase, which is a cause of tartar formation, Cholesterol in the liver by taking the recovery of the body works good for HDL-cholesterol, while the body is harmful to the body that acts to reduce the LDL-cholesterol also acts as a selective reduction action.

In addition, if you drink a lot of alcohol when you drink tea together with the various ingredients contained in the tea hangover is quickly resolved because the caffeine in the vitamin C, asparaginic acid and alanine amino acids by the alcoholic enzymes And it is known that the alcohol is rapidly excreted by the diuretic action of caffeine.

The method for obtaining the bud extract according to the present invention is not particularly limited, and preferably a method obtained from the bud of the tree or a method obtained by germinating the seed can be used.

The buds and larvae prepared by the above method can be directly or pulverized and extracted through a solvent to obtain the required extract. The solvent used here is not particularly limited and can be selected from commonly used solvents for extracting the components of the plant from the plant.

As the solvent to be used, for example, hot water or water; Lower alcohols such as methanol, ethanol, isopropyl alcohol and n-butanol; Polyhydric alcohols such as propylene glycol and 1,3-butylene glycol; Hydroformates of these alcohols: hydrocarbons such as n-hexane and toluene, and the like, but lower alcohol such as methanol or ethanol is preferably used as the extraction solvent.

When the lower alcohol is used as an extraction solvent, the obtained extract may be directly incorporated into the composition according to the present invention, but the extraction solvent may be distilled off and then dried and blended as necessary.

In one embodiment according to the present invention, the composition containing the bud extract and the larval extract is used in an amount of 0.001 to 10% by weight, preferably 0.001 to 10% by weight, based on the total weight of the composition, based on in vitro test and in vivo experiment May be contained in an amount of 0.01 to 5% by weight.

In addition, the compositions according to the present invention may be prepared in various forms of formulation.

In one embodiment, the composition according to the present invention may be a composition for oral administration. In addition, the composition according to the present invention may be a cosmetic composition, a health food composition or a pharmaceutical composition.

In one embodiment, the composition according to the present invention may be a cosmetic composition having a formulation of a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel or a skin adhesive type cosmetic.

In another preferred embodiment, the composition may be formulated into a transdermal dosage form such as a lotion, ointment, gel, cream, patch or spray.

In the composition of the various formulations, various substances may be selected and compounded according to the formulation or use purpose of the external preparation without any particular limitation in view of the technical skill of those skilled in the art, in addition to the essential constituents described above.

When the composition according to the present invention is applied to a pharmaceutical product, the extract can be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, soft capsules, powders, granules, powders, emulsions, syrups and pellets. have. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

In addition, the dosage of the active ingredient will vary depending on the age, sex, and weight of the subject to be treated, the specific disease or condition to be treated, the severity of the disease or condition, the route of administration, and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.001 mg / kg / day to approximately 2000 mg / kg / day. A more preferred dosage is 0.5 mg / kg / day to 2.5 mg / kg / day.

The present invention also provides a functional food or food additive comprising the above extract. Cheese, yogurt, juice, probiotics, and health supplements containing the composition including the fermented product of the obovate, and may be used in the form of various other food additives. The functional food or food additive containing the extract may exhibit various effects such as improvement of skin condition, inhibition of inflammation, antioxidation and anti-aging.

In one embodiment, the composition may contain other ingredients or the like that can give synergistic effects to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention.

For example, the addition amount of the above components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

In addition, the composition of the present invention may be in various forms such as solution, emulsion, viscous mixture, tablet, powder and the like, and may be administered by various methods such as simple drinking, injection, spraying or squeezing .

Hereinafter, the present invention will be described in further detail with reference to Examples and Experimental Examples. However, the following Examples are intended to illustrate the present invention and the scope of the present invention is not limited thereto.

[Example 1] An external preparation for skin containing a bud extract as an active ingredient

1-1. Preparation of bud extract and sprout extract

This embodiment is intended to effectively elute the active ingredients of buds, broccoli, oilseeds, sprouts, safflowers, cloves, flower buds, sprout buds, and tea buds, buds or germinated plants.

First, oilseed rape, clove, flower buds, siberian buds, and tea leaves were harvested between spring and summer, and stored frozen between minus 20 ° C and minus 30 ° C to prevent browning by enzyme reaction and the like.

Next, in the case of wheat, broccoli, sprouts and safflowers, the selected seeds are soaked in water for about 6 to 10 hours, then the cotton is spread on the culture vessel, the seeds are spread evenly over the non-overlapping seeds, Respectively. The sprouts were covered with a black cloth until about two-thirds of the time, and then they were left in the sunshine. Water was sprayed once or three times a day with a sprayer, and after 3 to 7 days, harvested and kept frozen at minus 20 ° C to minus 30 ° C.

The prepared sprouts and larvae were pulverized and then extracted with methanol as a solvent. The solvent was distilled off from the obtained extract to prepare a bud extract of each plant.

1-2. Preparation of composition for external application containing bud extract

To 100 g of the sprout and larvae extract prepared in Example 1-1, 100 ml of 95% hydrous alcohol was added to the dry weight. In order to prevent evaporation of the active ingredient, the active ingredient was extracted by heating at 50 to 100 DEG C for 10 hours in the state that the cooling condenser was mounted. The extracted components were filtered, concentrated under reduced pressure, and then dried to obtain a mixture of 5 g of sprout extract and sprout extract.

Using the obtained mixture, a composition for external application for skin was prepared with the composition shown in Table 1 below.

Concretely, raw materials 1 to 6 shown in Table 1 were first mixed and dissolved at 70 DEG C to form an aqueous phase, and raw materials 7 to 14 were dissolved at 70 DEG C to prepare an oil phase. Then, the oil phase was added to the water phase, followed by primary emulsification with a homomixer (Tokushu Kika, Japan), and then the raw material 15 was added to increase the oil phase. After removing the bubbles, the composition was cooled to room temperature.

Raw ingredient Addition amount One Purified water to 100 2 glycerin 8.0 3 Butylene glycol 4.0 4 Hyaluronic acid extract 5.0 5 Beta Glucan 7.0 6 Chamomile 0.1 7 (1: 1: 1: 1: 1: 1: 1: 1: 1, weight ratio, 0.5 8 Caprylic Capric Triglyceride 8.0 9 Squalane 5.0 10 Cetearyl glucoside 1.5 11 Sorbitan stearate 0.4 12 Cetearyl alcohol 1.0 13 antiseptic Suitable amount 14 incense Suitable amount 15 Triethanolamine 0.1

[Comparative Example 1]

The composition for external application was prepared in the same manner as in Example 1-2, except that no buds and larvae were added.

[Experimental Example 1] Observation of increased activity of skin epidermal stem cells

The keratinocytes isolated from the human epidermis were placed in a 24-well plate incubator at a concentration of 2 × 10 ⁵ , and then cultured in a DMEM medium containing 2% fetal bovine serum. Then, the buds and larvae extract prepared in Example 1 were replaced with a medium containing 50, 500 ppm, respectively, and cultured for 48 hours.

The negative control group was the one in which no sprout and larvae extract were added to the medium, and 10% fetal bovine serum was added to the medium instead of 2% fetal bovine serum. The supernatant was removed and the cells were washed twice with phosphate buffer solution and then 50 μl of cell lysis buffer (20 mM Tris-HCl (pH 7.4), 250 mM NaCl, 1% Triton X-100) And allowed to stand for 30 minutes. After washing twice with phosphate buffer solution, it was attached to the surface of the nitrocellulose membrane in a constant amount by using a vacuum apparatus.

The surface of the protein-coated surface was reacted with a blocking solution for 2 hours or more. The primary antibody of p63, a typical protein marker of epidermal stem cells, was suspended in a blocking solution at a ratio of 1: 500 and treated for 1 hour. .

The relative expression of p63 protein was measured as compared with the negative control to which no buds and larvae were added, and is shown in Table 2 below.

Test group Concentration (ppm) Relative Expression (%) Negative control group 100 Positive control group 120 Rapeseed 50 105.24 500 101.3 Clove sprout 50 98.68 500 103.84 Sprout 50 102.31 500 103.22 Germination 50 99.52 500 106.6 Germination broccoli 50 114.59 500 97.5 Germination bud 50 99.68 500 107.34 Germination safflower 50 89.11 500 84.68

Referring to Table 2, although the expression level of germinated safflower was slightly decreased, it was confirmed that the expression level of p63 protein was increased mostly by 7 kinds of buds and larvae extract. This means that the activity of epidermal stem cells is increased by the buds and larvae extract according to the present invention, and the buds and larvae extracts can enhance the epidermal stem cell power.

[Experimental Example 2] Observation of increase of skin cell proliferation by buds and larvae extract

2-1. Proliferation of keratinocytes

The keratinocytes isolated from the human epidermis were added to a 96 well plate incubator at a concentration of 5,000 per well and cultured in a KGM (keratinocyte growth medium, Lonza) medium for 24 hours. Then, the buds and larvae prepared in Example 1 were replaced with KBM (keratinocyte basal medium) containing 50 and 500 ppm, respectively, and cultured for 48 hours. The WST-1 solution (Roche) was added thereto at a ratio of 1/10, followed by reaction at 37 ° C for 2 hours in an incubator. The cell culture was quantitated by measuring the degree of color development at 450 nm in the incubated plate incubator. The results are shown in Table 3 below.

Raw material name Relative expression level (%) compared with control group 50 ppm 500 ppm Rapeseed 106 71 Clove sprout 111 107 Sprout 102 120 Germination 107 129 Germination broccoli 152 173 Germination bud 148 161 Germination safflower 158 119

Referring to Table 3, it can be seen that the proliferation of keratinocytes was promoted in each of the seven extracts. It was confirmed that the growth of keratinocytes in each of the buds and larvae was increased in the range of approximately 105 to 180%.

2-2. Proliferation of fibroblasts

The fibroblasts isolated from the human dermis were cultivated in a 96 well plate incubator for 24 hours at a concentration of 5000 per well and then cultured in DMEM medium (Welgene) containing 10% fetal bovine serum for 24 hours. Then, the buds and larvae extracts were replaced with DMEM medium containing 1% fetal bovine serum containing 50 and 500 ppm, respectively, and cultured for 48 hours. The WST-1 solution (Roche) was added thereto at a ratio of 1/10, followed by reaction at 37 ° C for 2 hours in an incubator. In the incubated plate incubator, the extent of cell proliferation was determined by measuring the degree of color development at 450 nm. The results are shown in Table 4 below.

Raw material name Relative expression level (%) compared with control group 50 ppm 500 ppm Rapeseed 96.32 91.81 Clove sprout 109.56 110.65 Sprout 106.36 108.05 Germination 116.69 134.08 Germination broccoli 85.99 106.85 Germination bud 102.75 124.56 Germination safflower 101.54 123.22

Referring to Table 4, it can be seen that fibroblast proliferation was promoted in each of the six extracts except rapeseed. It was confirmed that the proliferation of the fibroblasts was increased in the range of about 105 to 130% by the buds and larvae extracts.

[Experimental Example 3] Confirmation of suppression of collagen reduction by using a mouse model

In order to investigate the effect of skin wrinkle collagen degradation by ultraviolet rays, a coating test was carried out using a SKH-1 female hairless mouse at about 7 weeks of age. As shown in Table 1, Example 1 and Comparative Example 1 in which seven buds and larvae extracts were mixed were prepared and applied to the back of hairless mice for 11 weeks together with ultraviolet irradiation.

Ultraviolet irradiation was carried out every other day, and material application was carried out on a daily basis. After completion of the substance application, the skin of the back of the hairless mouse was biopsied to obtain proteins, and then the western blotting test for collagen was carried out to suppress collagen reduction by ultraviolet The results are shown in Fig.

1, it was confirmed that collagen reduction in the skin due to ultraviolet rays was restored when the combined treatment of seven kinds of buds and larvae extracts was compared with the control (Comparative Example).

According to the results of the above experimental examples, the composition containing the extract of the sprouts according to the present invention increases the activity of skin epidermal stem cells and promotes cell proliferation of epidermal keratinocytes and dermal fibroblasts.

In addition, the antioxidant effect, which is one mechanism of anti-aging, was also recognized, and it was confirmed that decomposition of collagen was delayed when 7 kinds according to one embodiment were used in combination. Such proliferation of the skin cells and promotion of the stem cell activity show that it can be used as an effective material for prevention and improvement of skin aging by improving the wrinkles and elasticity of the skin.

In addition, the present invention can contain the aloe leaf extract, centilla callus extract, and tangle extract in a composition for external application to the skin.

Here, aloe is mixed with various components such as polysaccharides, amino acids, vitamins, and tannins. Particularly polysaccharides are excellent in skin moisturizing properties, so they always function to maintain a constant water. Aloe's gelatinizes the molecules of monosaccharide, glucose and mannose Has a sticky adhesion to the dead cells of the outer skin to form a barrier to prevent foreign matter penetrating from the outside to prevent skin damage and moisturize the skin.

The aloe is extracted from the fresh green leaves of pure natural aloe. The extracted aloe jelly cleanses the skin, nourishes and moisturizes the skin, treats inflammation and eczema, and regenerates and whitens skin, blood circulation And to remove wrinkles.

Accordingly, when the aloe leaf extract is contained in the composition for external application for skin, aloe moisturizing, immunomodulating action, wound healing action, antibacterial / antiviral action and the like can be obtained.

It has also been known for a long time that it has been used as a cure for civilian disease. It is said that it has an excellent effect on wound healing, and ancient Indians are said to have eaten two sheets of leaflings every day when their skin is rough. In addition, Centella asiatica extract is used as a therapeutic agent which is very effective for surgical scratches and 2-3 degree burns. In the case of trauma, prescribe ointment containing 0.5-1.0% of asiaticoside to the wound caused by the skin disease, or 2% of the Asian ticoside is recommended dose and it is treated with one or two times a day.

Centella asiatica is the scientific name of the plant, which has been shown to promote the synthesis of collagen. The results of a rat experiment revealed that the centilla extract showed a wound healing effect of collagen and fibronectin in the dermis.

In terms of skin care, it plays an important role in helping the regeneration of aged skin. Especially, it is known that the product contains the centilla extract, which plays a role in coordinating itching and irritation.

In particular, in the present invention, it is preferable to use a wisteria fermentation extract, and a method for obtaining a wisteria fermentation extract is as follows. First, the leaflings and roots are washed and pulverized. Next, the pulverized material is injected into a ceramic material-made reactor in which air is permeated and the liquid component is not permeated, and then fermented by allowing it to stand at 25-28 캜 for 72 hours. By this fermentation, the pulverized seedlings are subjected to solid / liquid separation. The above fermented product of the Wakulpul isolate is filtered by a filter to obtain a Wakopulse fermented extract.

The inclusion of the centilla sprout extract of the present invention enables wound healing, collagen synthesis, anti-inflammation, and antibacterial activity to be further induced.

In addition, it is a fruit extract of Forsythia which is an Oleaceae plant. It mainly treats herbaceous, herbaceous, vulgaris, malaria, hypertrophy and solitary herpes in a herb, Detoxification, drainage, and anti-inflammation. In modern medicine, c-AMP phosphodiesterase is inhibited and used for antibacterial activity against Staphylococcus aureus.

In addition, the components of the allium include Lignans, Pinoresinol, Pinoresirnol glucoside Matairesinoside, Matairesinol, Phillyrin, Arctigenin, Caffeoyl glycoside, Forthiaside, Susensoside, Acteoside, Viridissimaside, Acetyl Oleanolic Acid (" Acetyloleanolic acid, Betulin, Viridissimic acid, Oleanolic acid, Betulinic acid, Rutin, Ursolic acid and the like.

The almond extract may be prepared by extracting dried almonds with an anhydrous or hydrolyzed alcohol having 1 to 3 carbon atoms or ethyl acetate at 4 to 30 ° C for 3 to 20 days, followed by concentration.

The almond extract can be prepared by extracting the allylate with water, anhydrous or hydrous alcohol having 1 to 3 carbon atoms or ethyl acetate at 50 to 100 ° C for 3 to 24 hours, and then concentrating.

By incorporating such a tangle extract into the composition for external application for skin according to the present invention, anti-aging, anti-inflammation, cyanotic detoxification, and antipyretic effect can be induced.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to those precise embodiments, and many alternatives, modifications, and variations will be apparent to those skilled in the art. I will understand.

Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (8)

As an active ingredient, a sprout extract composed of a bud sprout extract, a broccoli sprout extract, a rape bud sprout extract, a bud sprout extract, a safflower sprout extract and a clove sprout extract, a sprout extract of a flower bud, a sprout bud sprout extract and a tea leaf sprout extract A composition comprising a sprout extract and a sprout extract containing a sprout extract.
The method according to claim 1,
The sprout extracts can be used in an amount of 1: 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1, 5 < tb >< SEP >
The method according to claim 1,
The composition for external application for skin according to any one of claims 1 to 5, wherein the extract is a bud extract and the extract is from 1 to 5: 1 to 5: 1 to 5 parts by weight, based on the total weight of the extract.
The method according to claim 1,
The composition for external application for skin,
A composition for external application for skin using a bud extract and a sprout extract, wherein the sprout extract and the sprout extract are contained in a weight ratio of 1: 2 to 1: 2.
5. The method of claim 4,
Wherein the total weight of the bud extract and the bud extract is in the range of 0.001 to 10% by weight based on the total weight of the composition.
The method according to claim 1,
The composition for external application for skin using sprout extract and sprout extract prepared by extracting, filtering and concentrating under reduced pressure on hot water, C _{1 -4} lower alcohol or a mixture thereof,
The method according to claim 1,
The composition for external application for skin may be a formulation of a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel or a skin adhesive type cosmetic or a skin external application using a lotion, ointment, gel, cream, patch or spray extract Composition.
The method according to claim 1,
The external skin composition
Wherein the composition further comprises at least one selected from the group consisting of Aloe leaf extract, Centella asiatica, Centella asiatica extract, and Seaweed extract.

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