KR101270231B1 - 식물의 염 스트레스에 대한 저항성을 증가시키는 AtSZF2 유전자 및 이의 용도 - Google Patents
식물의 염 스트레스에 대한 저항성을 증가시키는 AtSZF2 유전자 및 이의 용도 Download PDFInfo
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Abstract
Description
도 2는 AtSZF2 유전자가 삽입된 콩 형질전환체의 생장 과정을 나타낸 것이다.
도 3은 AtSZF2 유전자의 삽입 여부를 알아보기 위해 수행한 PCR 결과를 나타낸 것이다. 각각의 숫자는 형질전환체 라인을 나타낸다. M: 마커(marker), PC: 양성 대조군(positive control), WT: 야생형(wildtype), EV: 공벡터(empty vector).
도 4는 AtSZF2 유전자의 삽입 여부를 알아보기 위해 수행한 RT-PCR 결과를 나타낸 것이다. 각각의 숫자는 형질전환체 라인을 나타낸다. WT: 야생형(wildtype).
도 5는 AtSZF2 유전자의 삽입 여부를 알아보기 위해 수행한 서던 블로팅 결과를 나타낸 것이다. 각각의 숫자는 형질전환체 라인을 나타낸다. WT: 야생형(wildtype).
도 6은 염화나트륨의 처리 시간(0, 4, 7, 11, 14 및 16일)에 따른 야생형 및 형질전환 식물체 잎의 표현형(A) 및 클로로필 함량(B)을 나타낸 것이다. WT: 야생형(wildtype), EV: 공벡터(empty vector), 4 및 6: 형질전환체 라인.
도 7은 염화나트륨의 처리 시간(0, 1, 3, 5, 7 및 10일)에 따른 야생형 및 형질전환 어린 식물체의 표현형(A), 이온 누출률(Ion leakage)(B) 및 클로로필 함량(C)을 나타낸 것이다. WT: 야생형(wildtype), EV: 공벡터(empty vector), 4 및 6: 형질전환체 라인.
도 8은 염 스트레스와 관련된 마커 유전자의 발현을 나타낸 것이다. 각각의 숫자는 형질전환체 라인을 나타낸다. WT: 야생형(wildtype), EV: 공벡터(empty vector), GmDREB2: Glycine max dehydration responsive element binding 2, GmDREB3: Glycine max dehydration responsive element-binding protein 3, GmOLPb: Glycine max Osmotin-Like Protein b, GmERF3: Glycine max AP2/ERF(ethylene response factor) type transcription factor, GmPHD2: Glycine max GmPHD(plant-homeo-domain)-type transcription factor 2, GmGT-2A: Glycine max trihelix transcription factor(GT-2A), GmGT-2B: Glycine max trihelix transcription factor(GT-2B), GmbZIP62: Glycine max basic/leucine zipper protein 62, GmWRKY54: Glycine max WRKY-type transcription factor 54, 18S: Glycine mas 18S ribosomal RNA.
Claims (9)
- 서열번호 1의 염기서열로 이루어진 애기장대(Arabidopsis thaliana) 유래 AtSZF2(Arabidopsis thaliana SALT-INDUCIBLE ZINC FINGER PROTEIN 2) 유전자를 포함하는 재조합 벡터를 대두 식물세포에 형질전환시켜 AtSZF2 유전자를 과발현하는 단계를 포함하는 대두 식물의 염 스트레스에 대한 저항성을 증가시키는 방법.
- 삭제
- 서열번호 1의 염기서열로 이루어진 애기장대(Arabidopsis thaliana) 유래 AtSZF2(Arabidopsis thaliana SALT-INDUCIBLE ZINC FINGER PROTEIN 2) 유전자를 포함하는 재조합 벡터로 대두 식물세포를 형질전환하는 단계; 및
상기 형질전환된 대두 식물세포로부터 대두 식물을 재분화하는 단계를 포함하는 야생형에 비해 염 스트레스에 대한 저항성이 증가된 대두 식물체의 제조 방법. - 삭제
- 제3항의 방법에 의해 제조된 야생형에 비해 염 스트레스에 대한 저항성이 증가된 형질전환 대두 식물체.
- 삭제
- 삭제
- 제5항에 따른 대두 식물체의 종자.
- 서열번호 1의 염기서열로 이루어진 애기장대(Arabidopsis thaliana) 유래 AtSZF2(Arabidopsis thaliana SALT-INDUCIBLE ZINC FINGER PROTEIN 2) 유전자를 포함하는, 대두 식물의 염 스트레스에 대한 저항성 증가용 조성물.
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GenBank Accession Number NM_129572 (2011.05.28.) * |
GenBank Accession Number NM_129572 (2011.05.28.)* |
Jiaqiang Sun 등. Plant Cell Physiol. 48(8), 페이지 1148-1158 (2007) * |
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