KR101107312B1 - Cosmetic composition for skin improvement and protection containing peptide - Google Patents

Abstract

The present invention provides a cosmetic composition for skin protection comprising dilauroyl glutamic acid lysine or a non-toxic salt thereof and N-acetylglucosamine as an active ingredient.

The cosmetic composition for skin protection according to the present invention has a synergistic effect in skin moisturizing and skin wrinkle improvement by a combination of dilauroyl glutamic acid lysine and N-acetylglucosamine, and almost no skin irritation, thereby maintaining skin health. Is excellent. In addition, the cosmetic composition has the effect of delaying skin aging by the moisturizing effect and wrinkle improvement effect, it can be used as functional cosmetics for skin moisturizing, wrinkle improvement, and skin aging prevention.

Description

A cosmetic composition for improving and caring skin comprising a peptide}

The present invention relates to a cosmetic composition for protecting the skin, and more particularly, including an amphiphilic peptide having a glycine-lysine-glycine peptide backbone and N-acetylglucosamine, which is safe without skin irritation and synergistic in improving skin moisturization and wrinkles. It relates to a cosmetic composition for protecting the skin.

In general, the skin is affected by various factors of the external and internal environment and changes to maintain homeostasis against ultraviolet rays, dry winds, heat, humidity, irritant detergents, physical illnesses, biochemical aging, and the like. In order to solve the problems of skin problems such as dry skin, pigmentation, wrinkles caused by such a change, cosmetics are used to maintain the skin's moisturizing power and improve wrinkles. The structure of the skin is composed of multiple structures of the stratum corneum, epidermis, dermis, and subcutaneous tissue from the outside, and cosmetics play an important role in particular involving the stratum corneum. In the stratum corneum, there are intercellular lipids such as natural moisturizing factor (NMF), which contains amino acids as a main ingredient, and ceramides and phospholipids, which function to prevent water evaporation.

Retinoids (Vitamin-A) and its derivatives, ascorbic acid (Vitamin-C) and its derivatives, peptides are produced by enhancing the production of collagen, elastin, hyaluronic acid and the like; Enzymes such as superoxide dismutases and catalases block disruption and induction pathways of epithelial or basal cells; In addition, fat-soluble, water-soluble antioxidants such as vitamin E, beta-carotene, glutathione, and ascorbic acid are known to maintain the skin's moisturizing ability by preventing the breakdown of the moisturizing membrane of the stratum corneum and by the new generation.

N-acetylglucosamine is a substance known for its skin moisturizing effect, and is a kind of natural amino sugar that can be obtained by decomposing high polysaccharide chitin contained in the shell of shellfish such as crabs and shrimps. N-acetylglucosamine is freely contained in milk at around 10 m / mL and is commonly present in vivo as a structural unit of the sugar chain portion of mucopolysaccharide, glycoprotein, and glycolipid. Normally, in vivo, glucose is used as a starting material, and is produced by metabolism, and is one of high safety biological components in humans. N-acetylglucosamine promotes the biosynthesis of mucopolysaccharides such as hyaluronic acid to enhance skin hydration, and has been proposed for use as a moisturizer and an antiwrinkle agent (Korean Patent Publication 2005-0078283, 2006-0123609) .

In addition, dilauroyl glutamate lysine has a peptide backbone (Glu-Lys-Glu) in which two lauroyl glutamate sodium salts, each of which is a single-chain, one hydrophilic group, are linked with lysine as a space, and show a gemini-like amphipathicity. ROSEN M, Geminis J., A NEW Generation of Surfactants, CHEMTECH, 23, 30 (1993). Dilauroylglutamic acid lysine is known to have the effect of restoring damaged skin and moisturizing skin, and to reduce skin irritation when used in combination with skin irritants (田村 幸 永, 山 脇 幸 男, 香 粧 粧 技2., 2. 25 (2007).

As discussed above, various materials may be used for protecting the skin, including moisturizing the skin and improving wrinkles. However, due to the limitation as a cosmetic composition that is not easy to penetrate the skin, it is not easy to see the excellent skin moisturizing and wrinkle improvement effect by applying the substances to the skin. Therefore, there is a constant demand for the development of a cosmetic composition having an improved pivo protection effect.

Accordingly, the present inventors studied to develop a cosmetic composition having a better effect on skin protection, including skin moisturizing and wrinkle improvement, and as a result, by combining a substance known to have a skin moisturizing and wrinkle improving effect, The present invention has been completed by finding a significant effect in terms of protective effect.

Accordingly, it is an object of the present invention to provide a cosmetic composition for skin protection including skin moisturizing and wrinkle improvement.

Another object of the present invention is to provide a method of applying the cosmetic composition to the skin to improve skin moisturizing or skin wrinkles.

In order to achieve the above object, the present invention provides a cosmetic composition for skin protection comprising dilauroyl glutamic acid lysine or a non-toxic salt thereof and N-acetylglucosamine as an active ingredient.

The present invention also provides a method for improving skin moisturizing or skin wrinkles comprising applying the cosmetic composition according to the present invention to the skin.

Hereinafter, the present invention will be described in more detail.

The cosmetic composition according to the present invention is a cosmetic composition for the first combination of dilauroyl glutamic acid lysine or a nontoxic salt thereof, and N-acetylglucosamine, which have been known in the prior art for protecting skin such as moisturizing effect and wrinkle improvement. As a result, each component synergistically increases the skin protection effect itself has, and shows a very good skin protection effect.

"Skin protection" as a use of the cosmetic composition according to the present invention is intended to improve the overall health of the skin, and specifically includes, but is not limited to, "moisturizing", "wrinkle improvement", or "combination of the above", etc. It doesn't happen. Therefore, according to a preferred embodiment of the present invention, the present invention provides a cosmetic composition for improving skin moisturization comprising (a) dilauroyl glutamic acid lysine or a non-toxic salt thereof and N-acetylglucosamine as an active ingredient, (b) di Cosmetic composition for improving skin wrinkles comprising lauroyl glutamic acid lysine or non-toxic salt thereof and N-acetylglucosamine as active ingredient, (c) dilauroyl glutamic acid lysine or non-toxic salt thereof and N-acetylglucosamine as active ingredient It provides a cosmetic composition for skin moisturizing and wrinkle improvement comprising as.

The non-toxic salt of dilauroyl glutamic acid lysine may be used in any salt form as long as it does not inhibit or exhibit the toxicity of dilauroyl glutamic acid lysine, and sodium dilauroyl glutamate is particularly preferred.

The composition of the present invention may preferably include 5 to 30 parts by weight of N-acetylglucosamine, more preferably 10 to 20 parts by weight based on 100 parts by weight of dilauroyl glutamic acid lysine, but is not limited thereto. The composition of the present invention may preferably comprise 0.01 to 10.0% by weight of lysine dilauroyl glutamate and 0.001 to 5.0% by weight of N-acetylglucosamine, and when it is less than the above range, the skin moisturizing and wrinkle improvement enhancing effect is weak. If it exceeds the above range, the cosmetic composition is not appropriate in terms of stability because the surface roughness, lumping is severe.

The composition of the present invention may be prepared in a specific formulation by a method well known in the cosmetic field, and may further include a cosmetically acceptable additive. Such additives include, but are not limited to, stabilizers, solubilizers, emulsions, vitamins, pigments, fragrances, or combinations thereof, and may vary depending on the type of formulation to be prepared.

The cosmetic composition for protecting the skin of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, shampoos, surfactant-containing cleansing It may be formulated as a liquid foundation and a cream foundation, but is not limited thereto. More specifically, it may be prepared in a formulation such as softening lotion, nourishing lotion, nutrient latex, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, hair essence, rinse.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer, or emulsion agent is used as a carrier component, for example, water, ethanol, carboxyvinyl polymer, propylene glycol, 1,3-butylene glycol, glycerin aliphatic Esters, polyethylene hardened castor oil, preservatives or sorbitan fatty acid esters.

When the dosage form of the present invention is a suspension, a liquid diluent such as water, fragrance, concentrated glycerin, butylene glycol or propylene glycol, ethoxylated iso terra teryl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan as a carrier component Emulsifying suspending agents such as esters, microcrystalline cellulose, carboxyvinyl polymers, thickeners or preservatives and the like can be used.

In the case where the formulation of the present invention is a surfactant-containing cleansing, as a carrier component, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like.

The cosmetic composition according to the present invention exhibits a synergistic effect on skin moisturization and skin wrinkle improvement by the combination of dilauroyl glutamic acid lysine and N-acetylglucosamine, as confirmed by experiments in the following examples. In addition, the cosmetic composition according to the present invention can be used safely as a cosmetic since the skin irritation is extremely low.

Therefore, in another aspect, the present invention provides a method of applying the cosmetic composition according to the present invention to the skin to improve skin moisturizing or skin wrinkles.

The cosmetic composition of the present invention may be applied by applying an appropriate amount to the skin according to the skin area to be applied, and may be used once to several times a day as needed. The amount and frequency of application can be increased or decreased as necessary according to the skin condition, age, etc. of the individual.

As described above, the skin care cosmetic composition according to the present invention exhibits a synergistic effect in skin moisturizing and skin wrinkle improvement by a combination of dilauroyl glutamic acid lysine and N-acetylglucosamine and almost no skin irritation. Excellent effect to keep healthy. In addition, the cosmetic composition has the effect of delaying skin aging by the moisturizing effect and wrinkle improvement effect, it can be used as functional cosmetics for skin moisturizing, wrinkle improvement, and skin aging prevention.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1: Make-up

As shown in Table 1, 0.1% by weight of carboxyvinyl polymer, 3.0% by weight of butylene glycol, 1.0% by weight of glycerin, 0.1% by weight of sodium dilauroyl glutamate, 0.01% by weight of N-acetylglucosamine, 0.5% by weight of PEG 4000 The mixture was heated, mixed and dissolved in purified water to prepare an aqueous mixture. Then, 0.2% by weight of polyoxyethylene cured castor oil, 0.2% by weight of polysorbate 80, 0.1% by weight of triethanolamine, methylparaben amount, and perfume amount in 6.0% by weight of ethanol were mixed with the aqueous mixture to make up the lotion. Prepared.

Comparative Examples 1, 2, and 3: make up lotion

Except for using the composition ratio shown in Table 1 in Example 1 was carried out in the same manner as in Example 1, to prepare a lotion of Comparative Examples 1, 2, and 3.

Example 2: Nutritional Milk Preparation

As shown in Table 2, 0.5 wt% vegetable wax, 0.5 wt% cetanol, 0.8 wt% cetostearyl alcohol, 0.6 wt% stearic acid, 0.6 wt% self-emulsifying monostearic acid, 0.5 wt% lipophilic monostearic acid, 0.50% by weight of beeswax, 0.5% by weight of paraffin wax, 3.0% by weight of squalane, 1.2% by weight of polysorbate 60, 0.8% by weight of sesquioleic acid, and fragrance were separately mixed to heat and dissolved to prepare an alcohol mixture.

Then, 0.1% by weight of N-acetylglucosamine, 1.0% by weight of sodium dilauroylglutamic acid lysine, 0.5% by weight of PEG 4000, 4.00% by weight of concentrated glycerin, 6.00% by weight of butylene glycol, 0.08% by weight of carboxypolymer, 0.08% of triethanolamine The nutrient emulsion was prepared by stirring and cooling the alcohol mixture by mixing the weight%, an appropriate amount of preservative, and the remaining amount of purified water with heat to dissolve it.

Comparative Examples 4, 5, and 6: Preparation of Nutrient Milk

Except for using the composition ratio shown in Table 2 in Example 2 was carried out in the same manner as in Example 2 to prepare the nutrient milk of Comparative Examples 4, 5, and 6.

Example 3: Nutrition Cream

As shown in Table 3 below, 1.0 wt% vegetable wax, 0.9 wt% cetanol, 1.00 wt% cetostearyl alcohol, 0.8 wt% stearic acid, 1.0 wt% self-emulsifying monostearic acid, 1.00 wt% lipophilic monostearic acid, Beeswax 1.50 wt%, squalane 6.00 wt%, paraffin wax 0.5 wt%, capric caprylic triglyceride 3.00 wt%, dimethicone 0.50 wt%, polysorbate 60 1.20 wt%, sorbitan stearate 0.8 wt%, and The fragrance was separately mixed and heated to prepare an alcohol mixture.

Then, 1.0% by weight of N-acetylglucosamine, 0.5% by weight of PEG 4000, 5.0% by weight of sodium dilauroyl glutamate, 6.00% by weight of concentrated glycerin, 6.00% by weight of butylene glycol, 0.1% by weight of carboxyvinyl polymer, triethanolamine The nutritional cream was prepared by stirring and cooling the said alcohol mixture in 0.10 weight% and the appropriate amount of preservatives by mixing with a residual amount of purified water and heating it.

Comparative Examples 7, 8, and 9: Nutritional Cream Preparation

The nutrition cream of Comparative Examples 7, 8, and 9 was prepared in the same manner as in Example 3, except that the composition ratios shown in Table 3 were used.

Experimental Example 1 Measurement of Moisturizing Force Using Coneometer CM 825

The moisturizing effect was measured using the Coneometer CM 825 (Corneometer CM 825, Courage + Khazaka) for the Examples and Comparative Example compositions. Five women in their 35's and 60's for each sample were divided into 8 sections of the upper arm 2 x 2 cm 2, and washed several times with water, and then water was removed without stimulation. After 15 minutes, the samples were applied evenly by dropping 10 µl into each of the two sections. After 2 hours, measurements were made 5 times per compartment using a coneometer. The experiment was conducted in a constant temperature and humidity room with a room temperature of 21 ± 2 ℃ and a relative humidity of 50 ± 5%. The measured values were used by averaging the values obtained by measuring five times, and are shown in Table 4 using the average value of five subjects. Table 4 shows the increase in the moisturizing power of Example 1, Comparative Examples 2, 3 for Comparative Example 1 to compare the increase in the moisturizing power of Example 1, Comparative Examples 2, 3 with respect to Comparative Example 1. In addition, Example 2, Comparative Examples 4-6, Example 3, and Comparative Example 7-9 are also shown in the same manner.

In Examples 1 to 3, the increase and decrease by the use of both sodium dilauroyl glutamic acid lysine and N-acetylglucosamine were used in Comparative Examples 2, 3, 5, 6, 8, and 9, respectively. It shows the increase and decrease of moisturizing ability. The increase in the moisturizing power of each of Examples 1, 2 and 3 is superior to the sum of the increase in the moisturizing power of Comparative Examples 2 and 3, the sum of the increase in the moisturizing power of Comparative Examples 5 and 6, and the increase in the moisturizing power of Comparative Examples 8 and 9, respectively. Was derived. Therefore, in terms of increasing the moisturizing power, it can be said that the formulations of Examples 1-3 of the present invention have a synergistic effect compared with the case of using sodium dilauroyl glutamic acid lysine or N-acetylglucosamine, respectively.

Experimental Example 2: Measurement of moisturizing power using a desiccator

For the Examples and Comparative Examples, the moisturizing effect was measured using the difference in the rate of moisture evaporation in the liquid phase as follows, and 30% of the Examples or Comparative Example formulations were dissolved in purified water, respectively.

After 10 g of the sample was titrated into a cup with a constant surface area, it was left for 24 hours in a desiccator adjusted to a temperature of 35 ° C. using completely dried calcium chloride (CaCl ₂ ) as an absorbent, and each sample was weighed 5 times after 24 hours. Moisturizing power was measured using the measured average value. The results are shown in Table 5.

According to the experimental results, the moisturizing power increase rate of Example 1 is greater than the sum of the increase rates of Comparative Examples 2, 3, the moisturizing power increase rate of Example 2 is greater than the sum of the increase rates of Comparative Examples 5, 6, the moisturizing power increase rate of Example 3 Was greater than the sum of the increase rates of Comparative Examples 8 and 9. Therefore, the formulations of Examples 1, 2, and 3 using both sodium dilauroyl glutamate and N-acetylglucosamine each have a synergistic effect on the moisturizing effect compared to the comparative examples when used alone. It is shown.

Experimental Example 3. Wrinkle improvement effect

Wrinkle improvement effect was measured using the composition prepared in Examples 1, 2, 3 and Comparative Examples 1-9.

50 women 35 to 60 years old were applied twice daily to the cosmetic compositions prepared in Examples 1, 2 and 3 and Comparative Examples 1-9 uniformly around the eyes, and the measurement area was wrinkled around the eyes. (crow's feet) was conducted for a total of three months.

The cosmetic composition prepared in Examples 1, 2 and 3 was applied to the left eye of the test subject, and the cosmetic composition prepared in Comparative Examples 1-9 was applied to the right eye. After 3 months, the degree of wrinkle relief was visually determined (judged by four stages of no improvement, slight improvement, moderate improvement, and significant improvement as compared with the control group) and is shown in Table 6 below, and before and after application of the cosmetic composition. Wrinkle template (replica) by measuring the depth of wrinkles (㎛) by image analysis method rm results are shown in Table 7 below.

According to Table 6, the improvement rate of the wrinkles is greater than the sum of the improvement rate increase for Comparative Example 1 of Comparative Example 1 of Comparative Example 1 of Example 1, the improvement rate of Comparative Example 4 of Example 2 The increase is greater than the sum of the improvement rate for Comparative Example 4 of Comparative Examples 5 and 6, and the increase rate of improvement for Comparative Example 7 of Example 3 is greater than the sum of the improvement rate for Comparative Example 7 of Comparative Examples 8 and 9. appear. Therefore, the formulations of Examples 1, 2, and 3 using both sodium dilauroyl glutamic acid lysine and N-acetylglucosamine each have a synergistic effect on the wrinkle improvement effect as compared to the comparative example when used alone. It was shown to have.

Further, according to Table 7, the increase in the wrinkle depth reduction rate for Comparative Example 1 of Example 1 in the depth of the wrinkles is greater than the sum of the increase in the wrinkle depth reduction rate for Comparative Example 1 of Comparative Examples 2 and 3, The increase in wrinkle depth reduction for Comparative Example 4 of 2 is greater than the sum of the increase in wrinkle depth reduction for Comparative Example 4 of Comparative Examples 5 and 6, and the increase in wrinkle depth reduction for Comparative Example 7 of Example 3 is greater than Comparative Example 8, It was found to be greater than the sum of the increase in the decrease in wrinkle depth for Comparative Example 7 of 9. Thus, the formulations of Examples 1, 2, and 3 using both sodium dilauroylglutamate lysine and N-acetylglucosamine each had a synergistic effect on the rate of wrinkle depth reduction compared to the comparative example when used alone. It was shown to have.

Experimental Example 3: Skin Safety Measurement

In order to evaluate the skin safety through the skin patch of the cosmetic composition prepared in Examples 1, 2, 3 and Comparative Examples 2, 3, 5, 6, 8, 9, the following experiment was performed. A total of 90 women (90 women) who had experienced skin side effects by cosmetics were applied to the patient's back with a cosmetic composition containing the cosmetic composition, and after 48 hours, the patch was removed, and then 1 hour to Primary determinations were made after 2 hours, and after 72 and 96 hours, the results are shown in Table 8 below.

According to Table 8, Examples 1, 2, and 3 according to the present invention are slightly safer than the cosmetic compositions prepared in Comparative Examples 2, 3, 5, 6, 8, and 9, and both the compositions of the Examples and the Comparative Example compositions are Significantly safe for skin.

Claims (9)

A skin protection cosmetic composition comprising dilauroyl glutamic acid lysine or a non-toxic salt thereof and N-acetylglucosamine as an active ingredient. The cosmetic composition according to claim 1, for moisturizing skin or improving skin wrinkles. The cosmetic composition according to claim 1, wherein the non-toxic salt of dilauroyl glutamic acid lysine is sodium dilauroyl glutamic acid lysine. The cosmetic composition according to claim 1, comprising 5 to 30 parts by weight of N-acetylglucosamine with respect to 100 parts by weight of the dilauroyl glutamic acid lysine. The cosmetic composition of claim 1, comprising 0.01 to 10.0 wt% of lysine dilauroyl glutamate and 0.001 to 5.0 wt% of N-acetylglucosamine. The composition of claim 1 further comprising a cosmetically acceptable additive. 7. The cosmetic composition according to claim 6, wherein the cosmetically acceptable additive is a stabilizer, a solubilizer, an emulsifier, a vitamin, a pigment, a perfume, or a combination thereof. The cosmetic composition of claim 1 which is a suspension, emulsion, cream, lotion, shampoo, surfactant-containing cleansing, liquid foundation, cream foundation, cleansing foam, cleansing water, hair essence, or rinse. delete