KR100794209B1 - Instant meal substitute using shellfish and its manufacturing method - Google Patents
Instant meal substitute using shellfish and its manufacturing method Download PDFInfo
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- KR100794209B1 KR100794209B1 KR1020060073292A KR20060073292A KR100794209B1 KR 100794209 B1 KR100794209 B1 KR 100794209B1 KR 1020060073292 A KR1020060073292 A KR 1020060073292A KR 20060073292 A KR20060073292 A KR 20060073292A KR 100794209 B1 KR100794209 B1 KR 100794209B1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L23/00—Soups; Sauces; Preparation or treatment thereof
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Abstract
본 발명은 패류를 이용한 인스턴트 식사대용식 및 그의 제조방법에 관한 것이다. 더욱 상세하게는, 패류 효소분해 핵심 엑스분을 향미반응시켜 풍미를 개선시키고 상기 풍미개선된 핵심 엑스분을 분쇄한 현미에 10 내지 15%(w/w)를 첨가한 후 현미 전분의 알파화 팽화공정을 이용하여 간편하고 영양가 높은 인스턴트 패류 식사대용식 및 그의 제조방법에 관한 것이다. The present invention relates to an instant meal substitute using shellfish and a method for producing the same. More specifically, the flavor of the shellfish enzymatic digestion is improved to improve the flavor, and after the addition of 10 to 15% (w / w) to the milled brown rice, the flavor-enhanced core extract is added and the alpha swelling of the brown rice starch is added. It relates to a simple and nutritious instant shell meal replacement formula and a method for producing the same using the process.
Description
도 1은 본 발명에 따른 패류 효소분해 엑스분의 제조방법을 도식화한 것이다.1 is a schematic diagram illustrating a method for preparing shellfish enzymatic digestion according to the present invention.
도 2는 본 발명 인스턴트 패류 식사대용식의 제조방법을 도식화한 것이다.Figure 2 is a schematic of the manufacturing method of the instant shellfish meal replacement formula of the present invention.
도 3은 본 발명 인스턴트 패류 식사대용식의 사진이다.Figure 3 is a photograph of the instant shellfish meal replacement meal of the present invention.
도 4는 본 발명 인스턴트 패류 식사대용식에 찬물을 첨가한 패류 맛죽의 사진이다.Figure 4 is a photograph of shellfish flavor porridge with cold water added to the instant shellfish meal replacement meal of the present invention.
본 발명은 패류 엑스분을 이용한 인스턴트 식사대용식 및 그의 제조방법에 관한 것이다. 보다 상세하게는 향미 개선된 패류 엑스분, 팽화시킨 쌀 전분 및 패류 동결건조물을 주원료로 하는 인스턴트 식사대용식 및 그의 제조방법에 관한 것이다.The present invention relates to an instant meal substitute using shellfish extract and a method for producing the same. More particularly, the present invention relates to an instant meal substitute having a flavor-improved shell meal, expanded rice starch, and shellfish lyophilized as a main ingredient and a method of manufacturing the same.
패류는 현재 약 10만 종 이상이 알려져 있으며 한국에서는 약 560종이 보고되어 있다. 바다, 담수, 육지에 살며 주로 깊은 바다에서 서식한다. 패류에는 양식할 수 있는 것이 많아 수산업상 중요한 동물 군이 있으며 양식하는 것에는 굴, 전복, 진주조개, 가리비 등이 있다. 패류 식품의 장점으로는 단시간에 손쉽게 조리할 수 있고, 저장이나 보존도 간단하며, 수송 및 휴대에 편리하다. There are currently over 100,000 species of shellfish and about 560 in Korea. Inhabits sea, fresh water and land, and lives mainly in deep sea. Many shellfish can be farmed, and there are important animal groups in fisheries. The farming includes oysters, abalone, pearl shells, and scallops. The advantages of shellfish are that they can be easily cooked in a short time, are easy to store and preserve, and are convenient for transportation and carrying.
인스턴트식품은 밥을 지어 녹말을 알파형으로 만든 다음, 80℃ 이상의 온도에서 건조시킨 것으로 끓는 물을 부으면 1 내지 5분 후에 섭취가 가능한 것을 말한다.Instant food is made of rice starch alpha type, and dried at a temperature of 80 ℃ or more, pour boiling water 1 to 5 minutes after ingestion.
지금까지 즉석죽, 선식 등과 같은 인스턴트식품은 농산물이나 생선류를 주원료로 제조하여 시판되고 있다. 그리고 전복 및 바지락 등 전통적으로 기능성과 풍미가 우수한 패류를 원료로 한 식품은 맛죽이나 레토르트 파우치 등의 제품이 출시되어 있으나, 현대인의 식생활 패턴에 맞는 간편하고 고영양성의 기능성 인스턴트 패류 식사대용식에 관한 연구나 제품은 현재까지 알려진 바가 없다. Until now, instant foods such as instant porridge and seonsik are being marketed by producing agricultural products or fish as a main raw material. In addition, foods made from shellfish and clams that have traditionally excellent functionality and flavor are available in products such as taste porridge and retort pouches. My product is unknown to date.
본 발명은 상기와 같은 점을 감안하여 안출된 것으로, 패류를 원료로 인스턴트 식사대용식 소재로서 활용될 수 있도록 풍미개선을 위한 향미방법 및 패류 엑스분, 쌀 전분의 팽화와 패류 동결건조물을 주원료로 하여 각종 첨가물의 첨가조건을 규명하고, 이의 활용가능성을 시판 유사 식품제품과 비교했을 때 풍미, 성분조성 및 관능미가 우수함을 확인함으로써 본 발명을 완성하였다.The present invention has been made in view of the above, the main method is the swelling and shellfish freeze-dried product of the flavor method for improving the flavor and shellfish X-starch, rice starch so that it can be utilized as a raw meal substitute shellfish as a raw material The present invention was completed by elucidating the addition conditions of various additives, and confirming that their applicability was superior to those of commercially available food products, in terms of flavor, composition, and sensuality.
따라서 본 발명의 목적은 패류의 효소분해법에 의해 제조된 풍미가 우수한 패류 핵심 엑스분을 제공하는데 있다.Accordingly, an object of the present invention is to provide a shellfish core extract with excellent flavor prepared by enzymatic digestion of shellfish.
본 발명의 다른 목적은 상기 패류 핵심 엑스분을 유효성분으로 하는 간편하고 영양가 높은 인스턴트 패류 식사대용식을 제공하는데 있다.Another object of the present invention is to provide a simple and nutritious instant shellfish meal replacement meal using the shellfish core extract as an active ingredient.
본 발명의 또 다른 목적은 상기 인스턴트 패류 식사대용식의 제조방법을 제공하는데 있다.Still another object of the present invention is to provide a method for preparing the instant shellfish meal replacement.
본 발명의 상기 목적은 바지락, 전복 등 패류를 원료로 효소를 불활성화시켜 풍미가 개선된 패류 핵심 엑스분을 추출하고 상기 패류 핵심 엑스분을 현미의 알파화 팽화 전분, 동결건조한 패류 분말 및 각종 첨가물을 혼합하여 인스턴트 패류 식사대용식을 제조함으로써 달성하였다.The above object of the present invention is to extract shell core extracts with improved flavor by inactivating enzymes with shellfish and abalone shellfish as raw materials, and the shellfish core extracts are alpha-expanded starch, freeze-dried shellfish powder and various additives of brown rice. It was achieved by mixing to prepare an instant shellfish meal replacement.
본 발명은 패류로부터 효소분해법 핵심 엑스분 추출 단계; 패류 핵심 엑스분의 향미반응 단계; 패류 핵심 엑스분의 관능검사 단계; 인스턴트 패류 식사대용식 제조 단계; 인스턴트 패류 식사대용식 품질 조성 및 일반성분 분석 단계 및 인스턴트 패류 식사대용식의 관능검사 단계를 포함하여 구성된다.The present invention is the enzymatic digestion core extracting step from shellfish; Flavor reaction of shellfish core extract; Sensory evaluation step of shellfish core extract; Instant shell meal replacement manufacturing step; Instant shell meal replacement quality composition and general component analysis step and instant shell meal replacement is configured to include a sensory test step.
본 발명에서 효소분해법 및 식사대용식으로 제조되는 패류는 패류 종류이면 어느 것이나 사용 가능하지만 본 발명에서는 일실시예로 바지락(Tapes philippinarum)을 사용하였다.Shellfish prepared by the enzymatic digestion method and meal replacement in the present invention can be any shell type, but in the present invention was used as an example of the clams ( Tapes philippinarum ).
본 발명에서 일반성분의 함량은 상법에 따라 수분은 상압가열건조법, 조단백질은 세미마이크로켈달(semi-micro Kjeldahl)법, 조지방은 속시렛(Soxhet)법, 회분은 건식회화법으로 측정하였다.In the present invention, the content of the general ingredients according to the conventional method, the moisture is atmospheric pressure drying method, the crude protein is semi-micro Kjeldahl method, crude fat was measured by Soxhet method, ash ash dry ash method.
본 발명에서 수분활성도(Aw)는 시료를 매우 잘게 세분화하여 수분활성도 측정기(Novasina MSL, Japan)를 이용하여 측정하였다.In the present invention, the water activity (Aw) was subdivided very finely into a sample and measured using a water activity meter (Novasina MSL, Japan).
본 발명에서 pH와 염도(salinity)는 시료에 10 배량의 순수를 가하여 균질기로 균질화한 후 pH는 pH meter(Fisher basic, USA)로써, 염도는 염도계(Istek 460CP, Korea)로써 측정하였다.In the present invention, pH and salinity (salinity) was added to the sample 10 times of pure water and homogenized with a homogenizer, pH was measured by pH meter (Fisher basic, USA), salinity was measured by a salinity meter (Istek 460CP, Korea).
본 발명에서 휘발성염기질소(Volatile basic nitrogen, VBN)는 Conway unit를 사용하는 미량확산법으로, 아미노질소(NH2-N)는 Formol법으로 정량하였다. In the present invention, volatile basic nitrogen (Volatile basic nitrogen, VBN) is a microdiffusion method using a Conway unit, amino nitrogen (NH2-N) was quantified by the Formol method.
본 발명에서 당-아미노반응(maillard reaction)에 의한 향미의 개선은 일정 농도의 바지락 효소분해 엑스분(SE, short-neck clam enzymatic hydrolyzate), 글루코스(glucose), 글리신(glycine) 및 시스틴(cystine)을 일정 비율의 중량비로 섞어 반응온도, 반응시간 및 반응액의 pH를 조정하여 당-아미노반응을 진행하였다. In the present invention, the improvement of flavor by the sugar-amino reaction (maillard reaction) is a certain concentration of short-neck clam enzymatic hydrolyzate (SE), glucose (glucose), glycine (glycine) and cystine (cystine) Was mixed in a proportion by weight to adjust the reaction temperature, reaction time and pH of the reaction solution to proceed with the sugar-amino reaction.
본 발명에서 TBA(Thiobarbituric acid value)값은 시료 5g를 정평한 후 수증기증류법으로 측정하였다. In the present invention, TBA (Thiobarbituric acid value) value was measured by steam distillation after 5g of the sample.
본 발명에서 안지오텐신-Ⅰ전환효소 저해능은 Cushman과 Cheung의 방법에 따라 시료 엑스분 50μL에 2% 안지오텐신-Ⅰ전환효소 조효소액 및 sodium borate buffer (pH 8.3) 용액 100μL를 가한 후 37℃에서 5분간 방치시켰다. 여기에 기질 로서 12.5mM Hippuryl-His-Leu 용액 50μL를 가하여 37℃에서 30분간 반응시킨 다음 1N 염산 용액 500μL를 가하여 반응을 정지시켰다. 다음 에틸아세테이트 1.5mL를 가하여 15초간 잘 교반한 후, 3,000rpm에서 5분간 원심분리시켜 상층액 1mL를 취한다. 이 상층액을 감압 데시케이터에 넣어 완전히 건조시킨 후 증류수 5mL를 가하여 용해시키고 228nm에서 흡광도를 측정하여 시료 엑스분 첨가 전후의 백분율로서 안지오텐신-Ⅰ전환효소 저해능을 측정하였다.In the present invention, the angiotensin-I converting enzyme inhibitory ability was added to 50 μL of sample X minutes according to the method of Cushman and Cheung, and 100 μL of 2% angiotensin-I converting enzyme coenzyme solution and sodium borate buffer (pH 8.3) solution were left at 37 ° C. for 5 minutes. I was. 50 μL of a 12.5 mM Hippuryl-His-Leu solution was added thereto and reacted at 37 ° C. for 30 minutes. Then, 500 μL of a 1N hydrochloric acid solution was added to stop the reaction. Next, 1.5 mL of ethyl acetate was added thereto, the mixture was stirred well for 15 seconds, and then centrifuged at 3,000 rpm for 5 minutes to take 1 mL of the supernatant. The supernatant was placed in a reduced pressure desiccator, dried completely, and dissolved in 5 mL of distilled water. The absorbance was measured at 228 nm, and the angiotensin-I converting enzyme inhibitory activity was measured as a percentage before and after the addition of the sample extract.
본 발명에서 유리아미노산 및 관련화합물은 시료 자숙농축액을 일정 비율로 희석한 다음 설포살리실릭산(5’-sulfosalicylic acid)를 10% 정도 첨가하여 하룻밤 방치한 후 여과하여 감압건조한 다음 0.20M 리티움 완충액(lithium buffer; pH 2.2)으로 보정한 후 아미노산 자동분석계(LKB-4150α, LKB Biochrom. Ltd., England)로 분석하였다.In the present invention, the free amino acid and related compounds are diluted with a certain amount of the sample concentrate, and then added with sulfosalicylic acid (5'-sulfosalicylic acid) about 10% overnight, filtered and dried under reduced pressure, followed by 0.20M litrium buffer solution. After calibration with (lithium buffer; pH 2.2), it was analyzed by an amino acid autoanalyzer (LKB-4150α, LKB Biochrom. Ltd., England).
본 발명에서 총아미노산은 시료에 6N HCl 용액을 넣어 열처리하여 24시간 분해 시킨 후 감압건고하고 citrate buffer(pH 2.20, 0.20M)로 정용한 후 아미노산 자동분석계(LKB-4150α, LKB Biochrom. Ltd., England)로써 측정하였다. In the present invention, the total amino acid was decomposed for 24 hours by adding 6N HCl solution to the sample, followed by heat treatment, and dried under reduced pressure, followed by definition with citrate buffer (pH 2.20, 0.20M), followed by amino acid automatic analyzer (LKB-4150α, LKB Biochrom. Ltd., England). Was measured.
본 발명에서 트리메틸아민옥시드(TMAO)와 트리메틸아민(TMA)는 Hashimoto와 Okaichi의 방법에 따라 분액여두에 제단백 및 탈지처리한 시료 엑스분 5mL에 중성 포르말린 용액 1mL, 톨루엔 10mL 및 50% 탄산칼륨 용액 3mL를 넣고 격렬하게 80 회 정도 교반한 다음 무수 망초를 넣어둔 시험관에 톨루엔 층만을 옮겨 탈수시키고, 이를 다시 다른 시험관에 취해 0.02% 톨루엔-피크린산 용액을 5mL 가해 혼합한 후 410nm에서 흡광도를 측정하여 트리메틸아민 함량을 구하였다. 한편, 시료 엑스분 5 mL, 5% 트리클로로아세트산 5mL와 10% 삼염화티타늄 용액 0.5mL를 25mL 정용플라스크에 넣어 2시간 방치 후 물로서 정용하고, 410nm에서 흡광도를 측정하여 (트리메틸아민옥시드+트리메틸아민) 함량을 구하였다. 여기에서 앞서 구한 트리메틸아민 함량을 빼어 트리메틸아민옥시드 함량으로 하였다. 총 크레아티닌(Total creatinine)은 제단백 및 탈지처리한 시료 엑스분 8mL를 취하여 시험관에 넣고 1.0 N 황산 용액을 1mL 첨가한 후 마개를 하여 121℃에서 30분간 분해시켰다. 냉각 후 m-니트로페놀 한방울, 0.1 N 가성소오다 용액 1 mL를 넣고, 실온에서 1시간 방치한 후 520 nm에서 UV 분광광도계 (Unicam Heλios, Unicam Ltd., England)로써 흡광도를 측정하여 검량곡선으로부터 총 크레아티닌의 함량을 구하였다.In the present invention, trimethylamine oxide (TMAO) and trimethylamine (TMA) are 1 mL of neutral formalin solution, 10 mL of toluene, and 50% potassium carbonate in 5 mL of samples extracted by deproteinization and degreasing in the separating funnels according to the method of Hashimoto and Okaichi. Add 3 mL of solution and stir vigorously for about 80 times, transfer only the toluene layer to a test tube containing anhydrous forget-me-not, dehydrate it, and add it to another test tube, add 5 mL of 0.02% toluene-picric acid solution, mix, and measure absorbance at 410 nm. Trimethylamine content was obtained. Meanwhile, 5 mL of sample X minutes, 5 mL of 5% trichloroacetic acid, and 0.5 mL of 10% titanium trichloride solution were placed in a 25 mL diaphragm flask and left for 2 hours, followed by standing as water. The absorbance was measured at 410 nm (trimethylamine oxide + trimethyl). Amine) content was obtained. The trimethylamine content calculated | required here was subtracted and it was set as trimethylamine oxide content. Total creatinine was taken in a test tube containing 8 mL of deproteinized and degreased sample extracts, 1 mL of 1.0 N sulfuric acid solution was added, and a stopper was decomposed at 121 ° C. for 30 minutes. After cooling, add 1 drop of m -nitrophenol and 0.1 mL of caustic soda solution, and leave at room temperature for 1 hour, and then measure the absorbance at 520 nm with UV spectrophotometer (Unicam Heλios, Unicam Ltd., England). Total creatinine content was determined.
본 발명에서 무기질 및 인의 정량은 질산을 이용하여 유기질을 습식 분해한 후 일정량으로 정용한 다음, 유도결합플라즈마 분광계(Inductively Coupled Plasma Atomic Emission Spectrometer; ICP, Atomscan 25, TJA Co., USA)로써 분석하였다. In the present invention, the determination of inorganic and phosphorus was carried out by wet decomposition of organic matter using nitric acid, and then fixed in an amount, followed by an inductively coupled plasma atomic emission spectrometer (ICP, Atomscan 25, TJA Co., USA).
본 발명에서 관능검사는 선식, 시판 죽 및 수프의 관능적 특성에 익숙하도록 훈련된 9명의 패널을 구성하여 인스턴트 패류 식사대용식 시제품의 맛, 조직감, 향기 및 종합적 기호도에 대하여 관능평가를 5단계 평점법으로 실시하였다. 이때 대조구로서는 시판용 쇠고기죽 및 수프을 사용하였으며, 대조구의 각 평가항목에 대한 관능평가점수를 3점으로 설정하고, 이를 기준으로 하여 5단계평점법 (5점: 아주 좋음, 4점: 좋음, 3점: 시판 쇠고기 죽과 동일, 2점: 나쁨, 1점: 아주 나쁨)으로 멸치 맛죽 시제품의 관능적 특성을 평가하였다. 관능검사 결과는 SPSS system (Statistical Package SPSS Inc. USA)을 이용하여 ANOVA test 및 Duncan's multiple range test로 p<0.05 수준에서 시료간의 유의성을 검정하였다.In the present invention, the sensory test consists of nine panels trained to be familiar with the sensory characteristics of wire, commercial porridge, and soup, and the sensory evaluation was performed on the taste, texture, fragrance, and overall acceptability of the instant shellfish meal replacement prototypes using a 5-step grading method. Was carried out. At this time, commercial beef porridge and soup were used as a control, and the sensory evaluation score for each evaluation item of the control was set to 3 points, and based on this, the 5-step scoring method (5 points: very good, 4 points: good, 3 points). : Sensory characteristics of anchovy-flavored porridge prototypes were evaluated with the same as commercial beef porridge, 2 points: bad, 1 point: very bad). The sensory test was performed using the SPSS system (Statistical Package SPSS Inc. USA) to test the significance between samples at the p <0.05 level using the ANOVA test and Duncan's multiple range test.
이하, 본 발명의 구체적인 구성 및 작용을 바람직한 실시예 및 실험예에 의해 더욱 상세히 설명한다.Hereinafter, the specific configuration and operation of the present invention will be described in more detail by preferred embodiments and experimental examples.
실시예1Example 1 : 바지락 효소분해 핵심 : Clapper Enzyme Core 엑스분X minutes 추출 extraction
도 1에서 도시된 바와 같이, 바지락의 핵심 엑스분은 하기와 같이 추출하였다. As shown in Figure 1, the core extract of the clam was extracted as follows.
초퍼(chopper)를 사용하여 바지락을 세절한 후 시료 중량에 대해 2배량의 물을 가하고, 95℃에서 5분간 자숙하여 자가소화효소를 불활성화시켰다. 상기에서 얻은 시료액을 pH 8.0으로 조정하고 상용효소 알카라제(alcalase) 0.6L을 시료 단백질량에 대해 0.5%(w/w)를 가하여 교반하면서 55℃에서 4시간 동안 1차 효소분해시켰다. 이를 다시 95℃에서 5분간 열처리하여 알카라제를 불활성화시킨 후 pH 6.0으로 조정하고 단백질분해효소인 플라보자임(flavourzyme)을 시료 단백질량에 대해 1.0%(w/w) 가하여 45℃에서 교반하면서 5시간동안 2차 효소분해시킨 후 다시 95℃에서 5분간 열처리하여 잔존효소를 불활성화 시켰다. 이 효소분해액을 여과포를 이용하여 잔사를 분리하고 부유지방층을 제거한 후, 고형분의 함량이 Brix 5~10°가 되도록 농축하여 바지락 효소분해 핵심 엑스분(short-neck clam enzymatic hydrolyzate)을 제조하였다.The clap was cut using a chopper, and twice the amount of water was added to the sample weight, and incubated at 95 ° C. for 5 minutes to inactivate autophagy. The sample solution obtained above was adjusted to pH 8.0, and 0.6L of the enzyme enzyme alcalase was added to 0.5% (w / w) based on the amount of the sample protein, followed by primary enzymatic degradation at 55 ° C. for 4 hours. This was further heat-treated at 95 ° C. for 5 minutes to inactivate the alkalase, and then adjusted to pH 6.0. Flavorzyme, a protease was added 1.0% (w / w) to the amount of the sample protein, and stirred at 45 ° C. After the second enzymatic degradation for 5 hours while heat treatment at 95 ℃ again for 5 minutes to inactivate the remaining enzyme. The enzymatic digestion solution was separated using a filter cloth to remove the suspended fat layer, and then concentrated to a solid content of Brix 5-10 ° to prepare short-neck clam enzymatic hydrolyzate.
실시예2Example 2 : : 패류Shellfish 핵심 main point 엑스분의Quarter 조향Steering 및 And 풍미개선을Improve flavor 위한 향미 반응( Flavor reaction for reactionreaction flavoringflavoring ))
표 1과 같이 Brix 5.0°의 바지락 효소분해 엑스분, 0.2M 글루코스(glucose), 0.2M 글리신(glycine) 및 0.2M 시스틴(cystine)을 중량비 4:2:1:1 비율로 섞어 반응온도 120℃, 반응시간 100분 및 반응액의 pH를 7.0으로 조정하여 당-아미노반응을 진행하였다. 상기의 반응조건은 향미 개선을 위한 당-아미노반응(maillard reaction)의 최적화 조건으로, 실시영역의 5단계 부호화, 중심합성계획, 반응표면 회귀분석, 통계분석 시스템(Statistical analysis system; SAS) 및 매스메티카 프로그램(mathematica program)을 사용하여 산출하였으며, 반응에 따른 돌연변이원의 생성 등을 고려하여 최적화 향미 반응 조건을 설정하였다.As shown in Table 1, a mixture of Brix 5.0 ° C. enzymatic digestion, 0.2M glucose, 0.2M glycine and 0.2M cystine were mixed at a weight ratio of 4: 2: 1: 1, and the reaction temperature was 120 ° C. , The reaction time was 100 minutes and the pH of the reaction solution was adjusted to 7.0 to proceed the sugar-amino reaction. The above reaction conditions are the optimization conditions of the sugar-amino reaction (maillard reaction) to improve the flavor, five-step coding, central synthesis scheme, response surface regression analysis, statistical analysis system (SAS) and mass It was calculated using the Mathematica program, and optimized flavor reaction conditions were set in consideration of the generation of mutagen according to the reaction.
<표 1> 향미 반응을 위한 패류 핵심 엑스분 당-아미노산 복합반응물Table 1 Shellfish Core X-min Sugar-Amino Acid Complex for Flavor Reaction
향미 반응(reaction flavoring)을 위한 당-아미노반응에 첨가할 아미노산은 Glycine+Cystine 복합첨가가 구수함과 단내 등의 관능적 특성이 인지되어 패류 엑스분과 혼합하여 당-아미노반응을 실시하였다. The amino acid to be added to the sugar-amino reaction for the reaction flavoring was recognized by the Glycine + Cystine complex addition and the organoleptic properties such as sweetness.
반응온도 120℃, 반응시간 100분 및 pH 7.1에서 향미 반응시킨 패류 엑스분을 5단계 평점법으로 관능검사한 결과를 표 2에 나타내었다. Table 2 shows the results of sensory evaluation of shellfish extracts reacted at a reaction temperature of 120 ° C., a reaction time of 100 minutes and a pH of 7.1 by a five-step scoring method.
상기 조건에서 향미 반응된 엑스분은 미반응 엑스분에 비해 냄새, 맛 및 종 합적 기호도 면에서 관능적 기호도가 상당히 증진되는 것으로 나타났다. The taste-reacted X-minutes under the above conditions were significantly improved in sensory preferences in terms of smell, taste, and overall palatability compared to the unreacted X-minutes.
<표 2> 바지락 효소분해 핵심 엑스분의 향미 반응 전후의 관능검사<Table 2> Sensory test before and after flavor reaction of clam enzymatic digestion core extract
실험예1Experimental Example 1 : 바지락 효소분해 핵심 : Clapper Enzyme Core 엑스분의Quarter 성분조성 및 기능특성 분석 Ingredient composition and functional characteristics analysis
Brix 2.0°으로 고형물의 양을 조정한 바지락 효소분해 엑스분의 일반성분 조성은 표 3과 같이 수분, 조회분 및 조단백질 함량은 각각 97.5%, 0.2% 및 1.3% 로 구성되었다.The general composition of the clam enzymatic digestion powder with the amount of solids adjusted to Brix 2.0 ° was 97.5%, 0.2% and 1.3%, respectively, as shown in Table 3.
<표 3> 바지락 효소분해 핵심 엑스분의 일반성분 조성(g/100mL)<Table 3> Composition of General Components of Glycolactate Core Extract (g / 100mL)
Brix 2.0°인 바지락 효소분해 엑스분의 pH, 염도, 산도 및 휘발성염기질소의 함량을 표 4와 같았다.Table 4 shows the pH, salinity, acidity, and volatile basic nitrogen content of Brix 2.0 deg.
엑스분의 pH와 염도는 각각 6.28 내외 및 0.15%, 산도는 0.98mL로 Brix 2.0°이라는 점을 고려할 때, 추출 중 유기산류의 용출이 비교적 많이 일어난 것으로 생각되었고 휘발성염기질소량은 2.1mg/100mL로서 역시 고형물량이 Brix 2.0° 인 점을 고려해 볼 때 추출 중 육성분의 분해로 인해 암모니아나 트리메틸아민 등이 비교적 많이 생성되는 것으로 나타났다. 따라서 이 효소분해 핵심 엑스분을 그대로 사용하는 것보다 당-아미노반응에 의한 향미 반응을 실시하여 풍미가 개선된 바지 락 효소분해 핵심 엑스분을 제조하여 본 발명에 사용하였다.Considering that the pH and salinity of the extracts were around 6.28 and 0.15%, respectively, and the acidity was 0.98 mL, Brix 2.0 °, elution of organic acids occurred relatively much during extraction and the amount of volatile basic nitrogen was 2.1 mg / 100 mL. Also, considering the Brix 2.0 ° solids content, ammonia and trimethylamine were relatively produced due to the decomposition of meat during extraction. Therefore, rather than using the enzyme digestion core extract as it is by performing a flavor reaction by the sugar-amino reaction to produce the clam enzymatic digestion core extract with improved flavor was used in the present invention.
<표 4> 바지락 엑스분의 pH, 염도, 산도 및 휘발성염기질소 함량TABLE 4 pH, salinity, acidity and volatile nitrogen content of clam extract
Brix 2.0°으로 고형물의 양을 조정한 바지락 효소분해 엑스분의 아미노질소 함량과 안지오텐신-Ⅰ전환효소 저해능을 측정한 결과를 표 5에 나타내었다. Table 5 shows the results of measuring the amino nitrogen content and angiotensin-I converting enzyme inhibitory ability of the clam enzymatic digestion obtained by adjusting the amount of solids to Brix 2.0 °.
바지락 효소분해 핵심 엑스분의 아미노질소 함량은 74.5mg/100mL로서 안지오텐신 저해능은 77.0±1.7%로서, 상당히 높은 안지오텐신-Ⅰ전환효소의 저해능을 나타내었다. 한편, 추출방법이 다른 바지락 엑스분과 비교하기 위해 98℃에서 4시간 열수로 추출한 열수고음 엑스분과 115℃에서 1시간 중탕고음한 중탕고음 엑스분을 함께 제조하여 아미노질소량과 안지오텐신-Ⅰ전환효소 저해능을 비교해 본 결과 하기 표 5과 같이 바지락 효소분해 엑스분이 아미노질소량과 안지오텐신-Ⅰ전환효소 저해능이 모두 월등히 높았다. 이는 효소분해 엑스분이 타 엑스분에 비해 육단백질이 분해되어 생성된 펩티드의 양이 많고 그 종류가 다양하기 때문이라고 생각되는데, 이 안지오텐신-Ⅰ전환효소 저해능은 엑스분 중의 펩티드 함량 및 종류와 관련이 있을 것으로 보아진다.The amino nitrogen content of the core extract of clam enzymatic digestion was 74.5 mg / 100 mL, and the angiotensin inhibitory activity was 77.0 ± 1.7%, indicating a very high inhibitory activity of angiotensin-I converting enzyme. On the other hand, in order to compare the extraction method with other clam extracts, hydrothermal treble extracts extracted with hot water at 98 ° C. for 4 hours and decocted treble extracts with 1 hour of soak at 115 ° C. were used to prepare amino nitrogen and angiotensin-I conversion enzyme inhibitory ability. As a result, as shown in Table 5, the extract of clam enzyme was significantly higher in both amino nitrogen and angiotensin-I converting enzyme inhibitory ability. This is thought to be due to the large amount and variety of peptides produced by the breakdown of hexaproteins compared to other extracts. This angiotensin-I converting enzyme inhibitory ability is related to the peptide content and type of extracts. It is expected to be.
<표 5> 바지락 효소분해 핵심 엑스분의 아미노질소 함량과 안지오텐신-Ⅰ전환효소 저해능Table 5 Amino nitrogen content and angiotensin-I converting enzyme inhibitory activity
Brix 2.0°으로 고형물의 양을 조정한 바지락 효소분해 핵심 엑스분의 유리아미노산 조성을 아미노산 자동분석계로써 측정한 결과는 표 6과 같았다.Table 6 shows the results of measuring the amino acid composition of the free amino acid composition of the clam enzymatic core X-mins, the amount of solids adjusted to Brix 2.0 °.
수산물 엑스분의 가장 중요한 정미발현성분으로 알려진 유리아미노산의 총량은 1,631.4mg/100mL로서, 주요 유리아미노산은 taurine, urea, asparagine glutamic acid, valine, leucine, lysine 및 arginine 등이었고, 이 외의 아미노산도 고루 함유되어 있었다. 이러한 유리아미노산 조성은 바지락 핵심 엑스분 맛의 강도와 맛의 발현 및 조화에 크게 기여할 것으로 본다. The total amount of free amino acid known as the most important net expression component of aquatic extracts is 1,631.4mg / 100mL. The major free amino acids are taurine, urea, asparagine glutamic acid, valine, leucine, lysine and arginine. It was. This free amino acid composition is expected to greatly contribute to the strength and flavor expression and harmony of the clam core extract.
<표 6> 바지락 효소분해 핵심 엑스분의 유리아미노산 함량 (mg/100mL)<Table 6> Free amino acid content of core extract of clam enzymatic digestion (mg / 100mL)
Brix 2.0°으로 고형물의 양을 조정한 바지락 효소분해 핵심 엑스분의 유기염기성분을 측정한 결과는 표 7과 같았다. 수산물 엑스분의 시원한 감미에 관여하고 수산생물의 삼투압을 조절하는 성분인 트리메틸아민옥시드, 이의 환원물질인 트 리메틸아민 및 수산물 엑스분의 떫은맛에 관여하는 성분인 총 크레아티닌은 각각 3.9mg/100mL, 0.4mg/100mL 및 1.6mg/100mL 정도로 양적인 면으로 보아 바지락 엑스분 맛의 발현에 미치는 영향은 그다지 크지 않은 것으로 확인하였다.Table 7 shows the results of measuring organic base components of the clam enzyme digestion core X-min, the amount of solids adjusted to Brix 2.0 °. Trimethylamine oxide, which is involved in the cool sweetness of seafood extracts and controls the osmotic pressure of aquatic organisms, total creatinine, which is a component involved in the astringent taste of trimethylamine oxide, its reducing substance, and seafood extract, is 3.9mg / 100mL. In terms of quantity, 0.4mg / 100mL and 1.6mg / 100mL were found to have little effect on the expression of clam extract.
<표 7> 바지락 효소분해 핵심 엑스분의 유기염기 함량(mg/100mL)<Table 7> Organic base content (mg / 100mL) of core extract of clam enzymatic digestion
Brix 2.0°으로 고형물의 양을 조정한 바지락 효소분해 핵심 엑스분의 무기질 조성을 유도결합플라즈마 분광계로 분석한 결과는 표 8과 같았다. 바지락 엑스분에는 무기이온으로서 Na, Mg 및 Ca이 양적으로 많았으며, 그 외 Fe, Zn, S, K, P 등도 미량 함유되어 있었다. 바지락 효소분해 핵심 엑스분의 무기이온 조성을 다른 수산물 엑스분과 비교해 볼 때 칼슘의 함량이 많은 반면, 칼륨 및 인의 함량이 적은 점이 특징이었다.The mineral composition of the clam enzymatic core X-min, the amount of solids adjusted to Brix 2.0 °, was analyzed using an inductively coupled plasma spectrometer. In the clam extract, Na, Mg and Ca were quantitatively high as inorganic ions, and Fe, Zn, S, K, and P were also contained in trace amounts. Compared with the other seafood extracts, the inorganic ion composition of the clam enzyme digestion core extracts was high in calcium and low in potassium and phosphorus.
<표 8> 바지락 효소분해 핵심 엑스분의 무기이온 함량(mg/100mL)<Table 8> Content of inorganic ion (mg / 100mL)
실험예2Experimental Example 2 : 바지락 효소분해 핵심 : Clapper Enzyme Core 엑스분의Quarter 관능특성 및 수율 Sensory properties and yield
수산물 엑스분에 익숙하도록 훈련한 9인의 관능검사원을 조직하여 상기에서 제조한 바지락 열수고음, 중탕고음 엑스분과 효소분해 엑스분의 냄새, 맛 및 종합 적기호도에 대해 관능검사한 결과 및 원료 바지락 육 1kg에서 얻어지는 Brix 2.0°의 엑스분 수율을 표 9에 나타내었다.Nine sensory testers trained to be familiar with seafood extracts, organoleptic hot water, biscuit, and enzymatic digestion of odor, taste, and comprehensive redness of the clam extract, and raw clam meat 1kg The x-minute yield of Brix 2.0 ° obtained in Table 9 is shown.
냄새, 맛 및 종합적 기호도 면에서는 바지락 효소분해 핵심 엑스분이 상기 열수고음, 중탕고음 엑스분에 비해 월등히 높은 평점을 받았다. 한편, 바지락 육 1kg에서 얻어지는 Brix 2.0° 엑스분의 수율은 바지락 효소분해 핵심 엑스분이 6.58L, 열수고음이 2.65L, 중탕고음이 1.80L로서 바지락 효소분해 핵심 엑스분의 수율이 상기 타 엑스분에 비해 3배 정도 높은 것으로 나타났다.In terms of smell, taste, and overall acceptability, the clam enzymatic digestion core excellence score was significantly higher than that of the hydrothermal treble and spout. On the other hand, the yield of Brix 2.0 ° X minutes obtained from 1 kg of clam meat was 6.58L for clam enzyme digestion, 2.65L for hot water treble, and 1.80L for hot water treble. 3 times higher than that.
따라서 바지락 육으로부터 본 발명에 사용되는 바지락 핵심 엑스분 소재를 제조하려면 여러 가지 추출법 중에서 효소분해법이 관능적인 면에서 가장 적합함을 알 수 있었다.Therefore, from the clam meat, it was found that the enzymatic digestion method was most suitable in terms of sensuality among the various extraction methods in order to manufacture the clam core x-minute material used in the present invention.
<표 9> 바지락 효소분해 핵심 엑스분의 관능검사 및 수율<Table 9> Sensory Evaluation and Yield of Core Extracts
실시예3Example 3 : 본 발명 인스턴트 Instant invention 패류Shellfish 식사대용식의 제조 Preparation of Meal Replacement
도 3과 같이 본 발명 인스턴트 패류 식사대용식을 하기와 같이 제조하였다. 본 발명 인스턴트 패류 식사대용식의 소재인 분쇄한 현미에 상기 제조한 패류 효소분해 핵심 엑스분을 10 내지 15% 첨가한 후 스크류 팽화기(디스크플레이트 온도 130℃, 압력 40 중량부/cm2)를 이용하여 간단히 물만 부어도 호화시킬 수 있는 패류 엑스분 첨가 알파화 전분을 가공하였다. 상기 패류 엑스분 첨가 알파화 전분에 표 10과 같은 조성의 첨가물을 혼합하여 인스턴트 패류 식사대용식을 제조하였다. Instant shellfish meal replacement formula of the present invention as shown in Figure 3 was prepared as follows. 10 to 15% of the shellfish enzymatic digestion core extract prepared above was added to the pulverized brown rice which is a substitute for instant shellfish meal, and then, using a screw expander (disc plate temperature 130 ° C., pressure 40 parts by weight / cm 2). Alpha-starch added with shellfish extract, which can be gelatinized simply by pouring water, was processed. Instant shellfish meal replacement formula was prepared by mixing the additives of the composition as shown in Table 10 to the shellfish extract starch-added alpha starch.
상기 실시예3에 따라 제조된 본 발명 인스턴트 패류 식사대용식에 물을 부어 혼합한 결과 도 4와 같이 호화되었다.Water was poured into the instant shellfish meal replacement formula of the present invention prepared according to Example 3 and mixed as shown in FIG. 4.
<표 10> 본 발명 인스턴트 패류 식사대용식의 첨가물 조성 Table 10: Additive Composition of Instant Shell Meal Replacements of the Present Invention
실험예3Experimental Example 3 : 본 발명 인스턴트 Instant invention 패류Shellfish 식사대용식 품질의 성분 분석 Component Analysis of Meal Replacement Quality
본 발명 인스턴트 패류 식사대용식의 일반성분, 구성아미노산 및 무기질 조성을 각각 표 11 내지 13에 나타내었다. 본 발명 인스턴트 패류 식사대용식의 수분 함량은 6.4%, 조단백질은 14.7%로서 단백질 함량이 많은 고단백식품임이 확인되었으며 이는 본 발명 인스턴트 패류 식사대용식의 제조 중 첨가된 바지락 효소분해 엑스분 및 바지락 동결건조물의 영향 때문으로 판단된다. 인스턴트 패류 식사대용식 시제품은 하기 표 11과 같이 수분 함량이 매우 적고 지방 함량이 5.8%로 비교적 많이 함유되었다. General ingredients, constituent amino acids and mineral compositions of the instant shellfish meal replacement formula of the present invention are shown in Tables 11 to 13, respectively. Moisture content of the instant shellfish meal replacement meal of the present invention was 6.4%, crude protein is 14.7%, it was confirmed that the protein is a high protein food with a lot of protein content of the instant shellfish meal replacement formula and the effect of the added clam enzymatic digestion and clam lyophilized during the preparation of the instant shellfish meal replacement formula It is because of this. The instant shellfish meal replacement prototype contained a relatively low moisture content and a relatively high fat content of 5.8% as shown in Table 11 below.
<표 11> 본 발명 인스턴트 패류 식사대용식의 일반성분 조성(g/100g)Table 11 General ingredient composition (g / 100g) of the instant shellfish meal replacement formula of the present invention
본 발명 인스턴트 패류 식사대용식의 구성아미노산은 하기 표 12와 같이 총 함량은 14,066mg/100g으로, 곡류를 주식으로 하는 우리나라 사람들에게 부족하기 쉬운 리신(Lys)을 비롯하여 아스파르트산(Asp), 글루탐산(Glu), 류신(Leu), 페닐알라닌(Phe) 및 아르기닌(Arg)이 각각 1% 이상 함유되어 있었으며, 이외의 다른 아미노산들도 비교적 고루 함유되어 있었다. The amino acid composition of the instant shellfish meal replacement formula of the present invention has a total content of 14,066mg / 100g as shown in Table 12 below, as well as lysine (Lys), aspartic acid (Asp) and glutamic acid (Glu), which are not enough for Korean people who have grain as a staple food. ), Leucine (Leu), Phenylalanine (Phe) and Arginine (Arg) contained more than 1%, and other amino acids were relatively evenly contained.
<표 12> 본 발명 인스턴트 패류 식사대용식의 구성아미노산 함량 (mg/100 g)<Table 12> Compositional Amino Acid Content (mg / 100 g) of the Instant Shell Meal Replacement Formula of the Present Invention
본 발명 인스턴트 패류 식사대용식의 무기질 조성은 하기 표 13에서와 같이 첨가된 죽염의 영향으로 Na 성분이 3,353mg/100g으로 가장 많았으며, 다음이 K, P, Mg, Ca, S 등이 고루 함유되어 있었다. 이들은 인체에 흡수되기 쉬운 이온의 형태로서 본 발명 인스턴트 패류 식사대용식을 섭취할 경우 인체에 필요한 무기질을 충분히 공급할 수 있을 것으로 평가되었다.The mineral composition of the instant shellfish meal replacement formula of the present invention was the most Na component 3,353mg / 100g under the influence of bamboo salt added as shown in Table 13, the following is evenly contained K, P, Mg, Ca, S, etc. there was. These were evaluated to be able to supply sufficient minerals to the human body when the instant shellfish meal replacement meal of the present invention in the form of ions easily absorbed by the human body.
<표 13> 본 발명 인스턴트 패류 식사대용식의 무기질 함량 (mg/100g)Table 13 Mineral Contents of the Instant Shell Meal Replacements of the Present Invention (mg / 100g)
실험예4Experimental Example 4 : 본 발명 인스턴트 Instant invention 패류Shellfish 식사대용식의 일반성분의 함량 분석 Analysis of Contents of Common Components in Meal Replacement
본 발명 인스턴트 패류 식사대용식의 제품화를 위해 총지방질 함량 등 일반성분을 분석한 결과 표 14에 나타내었다.Table 14 shows the results of analyzing the general components such as total fat content for the production of instant shellfish meal replacement formula of the present invention.
<표 14> 본 발명 인스턴트 패류 식사대용식의 시판을 위한 일반성분 함량Table 14 Contents of General Ingredients for Commercialization of Instant Shellfish Meal Replacements of the Present Invention
실시예4Example 4 : 본 발명 : The present invention 인스탄트Instant 바지락 식사대용식의 관능검사 Sensory test of clam meal substitute
본 발명 인스턴트 패류 식사대용식의 관능적 특성인 맛, 조직감, 향기 및 종합적 기호도에 대하여 관능평가를 5단계 평점법으로 실시한 결과는 표 15와 같았다. 본 발명 인스턴트 패류 식사대용식은 유사 시판제품에 비해 간편성에서는 월등히 뛰어난 평점을 얻었으며 맛, 조직감, 향기 및 종합평가에서도 유사 시판 제품 2종에 비해 높은 평점을 받았다.The results of sensory evaluation using a five-step scoring method on the sensory characteristics, texture, aroma, and overall acceptability of the instant shellfish meal replacement formula of the present invention are shown in Table 15. Instant shellfish meal replacement formula of the present invention obtained a superior rating in simplicity compared to similar commercial products, and received high ratings compared to two similar commercial products in taste, texture, aroma and comprehensive evaluation.
<표 15> 본 발명 인스턴트 패류 식사대용식의 관능검사Table 15: Sensory test of instant shellfish meal replacement formula of the present invention
이상의 실시예와 실험예를 통하여 설명한 바와 같이, 본 발명은 풍미 개선을 위한 향미 반응된 패류 핵심 엑스분, 알파화 쌀전분 및 패류 동결건조물을 원료로 맛, 냄새 및 종합적 기호도 면에서 뛰어난 효과가 있는 인스턴트 패류 식사대용식 및 그 제조 방법에 관한 것이다. 상기 방법에 의해 제조된 본 발명 인스턴트 패류 식사대용식은 유사 시판제품과 비교하여 맛, 조직감, 향기, 간편성 등 관능적면에서 뛰어난 효과가 있으므로, 본 발명 인스턴트 패류 식사대용식 및 그의 제조방법은 식품산업상 매우 유용한 발명인 것이다.As described through the above Examples and Experimental Examples, the present invention has excellent effects in terms of taste, smell and overall acceptability as raw materials of flavor-reacted shellfish core extract, alpha-ized rice starch and shellfish lyophilisate for flavor improvement. Instant shell meal substitutes and a method for producing the same. Since the instant shellfish meal replacement formula of the present invention prepared by the above method has an excellent effect in terms of taste, texture, fragrance, and simplicity compared to similar commercial products, the instant shellfish meal replacement formula of the present invention and its manufacturing method are very It is a useful invention.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101073199B1 (en) | 2011-05-20 | 2011-10-12 | 김미숙 | Instant porridge manufacturing method |
KR101146886B1 (en) * | 2009-08-27 | 2012-05-16 | 강릉원주대학교산학협력단 | A method for preparing the instant food using by Patinopecten yessoensis |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5712980A (en) | 1980-06-26 | 1982-01-22 | Tsunezo Goto | Preparation of portable nutrient food |
KR20030005503A (en) * | 2001-07-09 | 2003-01-23 | 조양호 | Chinese medicine health seeking abalone porridge making uncleaned rice and various grains its main material and manufacture method thereof |
KR20040033500A (en) * | 2002-10-14 | 2004-04-28 | 손종업 | Manufacturing method of instant porridge using natural seasoning |
KR20040072785A (en) * | 2003-02-11 | 2004-08-19 | 양천중 | The method for manufacturing instant abalone-gruel |
KR20050097265A (en) * | 2004-04-01 | 2005-10-07 | 장흥군 | A |
KR20050097266A (en) * | 2004-04-01 | 2005-10-07 | 장흥군 | A |
KR20060102788A (en) * | 2005-03-25 | 2006-09-28 | 이민성 | Food composition mainly from brown rice |
-
2006
- 2006-08-03 KR KR1020060073292A patent/KR100794209B1/en not_active Expired - Fee Related
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5712980A (en) | 1980-06-26 | 1982-01-22 | Tsunezo Goto | Preparation of portable nutrient food |
KR20030005503A (en) * | 2001-07-09 | 2003-01-23 | 조양호 | Chinese medicine health seeking abalone porridge making uncleaned rice and various grains its main material and manufacture method thereof |
KR20040033500A (en) * | 2002-10-14 | 2004-04-28 | 손종업 | Manufacturing method of instant porridge using natural seasoning |
KR20040072785A (en) * | 2003-02-11 | 2004-08-19 | 양천중 | The method for manufacturing instant abalone-gruel |
KR20050097265A (en) * | 2004-04-01 | 2005-10-07 | 장흥군 | A |
KR20050097266A (en) * | 2004-04-01 | 2005-10-07 | 장흥군 | A |
KR20060102788A (en) * | 2005-03-25 | 2006-09-28 | 이민성 | Food composition mainly from brown rice |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101146886B1 (en) * | 2009-08-27 | 2012-05-16 | 강릉원주대학교산학협력단 | A method for preparing the instant food using by Patinopecten yessoensis |
KR101073199B1 (en) | 2011-05-20 | 2011-10-12 | 김미숙 | Instant porridge manufacturing method |
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