KR100613266B1 - Keratin exfoliation accelerator and pharmaceuticals or cosmetics containing it as an active ingredient - Google Patents

Abstract

The present invention overcomes side effects and stability problems such as alpha hydroxy acid (AHA) and trichloroacetic acid, which are known to date, have no toxicity and have good stability, and have excellent exfoliation effect than existing substances. As an accelerator, 5,15-diacetoxy-3-phenylacetoxy-14-oxoratyadiene-6 (17) -epoxide represented by the following general formulas (I) and (II), respectively (Euphorbia factor L1) And 5,15-diacetoxy-3,7-dibenzoyloxy

At least one compound selected from the group consisting of -14-oxorathiadiene (Euphorbia factor L2).

[Formula I]

[Formula II]

Keratin exfoliation accelerator, keratin, desmosome, keratin stratification, alpha hydroxy acid, trichloroacetic acid, water soluble, euphorbia factor L1, L2

Description

Keratin peeling accelerator and pharmaceuticals or cosmetics containing the same as an active ingredient {STRATUM CORNEUM DELAMINATION PROMOTING AGENT AND MEDICINE OR COSMETIC MATERIAL MATERIAL CONTAINIG THE SAME}

[Industrial use]

TECHNICAL FIELD The present invention relates to a keratin exfoliation accelerator, and more particularly, 5,15-diacetoxy-3-phenylacetoxy-14-oxoratyadiene- represented by the following general formulas (I) and (II), respectively.

6 (17) -epoxide (5,15-diacetoxy-3-phenylacetoxy-14-oxolathyradiene-6 (17)

-epoxide), and 5,15-diacetoxy-3,7-dibenzoyloxy-14-oxoratiadiene (5,15-

It relates to a keratin exfoliation promoter selected from diacetoxy-3,7-dibenzoyloxy-14-oxolathyradiene) and to pharmaceuticals or cosmetics containing the same as an active ingredient.

[Formula I]

[Formula II]

[Prior art]

Generally, the skin is divided into three parts from the upper layer of skin: epidermis, dermis and subcutaneous fat layer. Of these, the epidermis is further subdivided into the stratum corneum, granule layer, polar layer, and basal layer. The epidermal cells of the basal layer are differentiated as they rise to the upper part of the skin and finally reach the stratum corneum. Epidermal cells that reach the stratum corneum lose their nuclei and are converted to dead cells as they are filled with insoluble proteins called keratin. The stratum corneum consists of differentiated epidermal cells (skin cells) and the skin lipids that fill them in. They protect defense against external physical, chemical and biological stimuli and protect the body from external physical, chemical and biological stimuli. do. Keratinocytes are connected by a protein called desmosome, and as they rise up the stratum corneum, desmosomes are broken down, weakening cohesion between keratinocytes and finally keratinocytes are separated from the skin. In normal skin, the stratum corneum consists of 15-20 layers and it takes 15-20 days for them to be completely separated (British Journal of Dermatology, 86, 14-19, 1974; KM Halprin, Fragrance Society, 12 (4), 265-271, 1988; M. Takahashi).

Aging skin, dry skin, acne skin and the like, the separation of the stratum corneum is slower than normal, the stratum corneum is thickened (stratum corneum stratification) phenomenon, the outstanding feature that appears in the appearance of the skin is the scale of the skin (scale). The stratum corneum stratification is mainly due to decreased skin moisturizing ability, decreased production and activity of desmosome degrading enzymes, decreased cell activity, and skin aging, UV exposure, and pollution. Artificially thinning the stratum corneum thickened by these internal and external factors externally increases the activity or regeneration of living cells under the stratum corneum, reducing skin scales appearing on the appearance of the skin, softening the skin, removing wrinkles, suppressing the occurrence of acne, and There is a lot of research to solve the stratum corneum stratification within the range that does not cause irritation due to the effects of treatment. To relieve keratinization, physically rub the skin or use chemical dermabrasion. In particular, chemical peels have additional effects such as fine wrinkles, rough skin relief and blemishes. Substances used for chemical peeling include trichloroacetic acid, phenol, and alpha hydroxy acid (AHA). The concentration of alpha hydroxy acid used for chemical peeling is used at a high concentration of at least 20-30%, but at low concentrations below 10%, the stratum corneum is slowly exfoliated (Journal of American Academy of Dermatology, 11, 867-879, 1984; Van Scott et al.), Enhancing skin moisturization (Happi, July, 66-68, 1994; Tom et al.), And reducing fine lines (Cutis, 43, 222-228, 1989; Van Scott et al.). Many products are on the market. In addition, it is known that it is possible to treat and prevent acne by removing keratin that narrows facial hair follicles by promoting keratin exfoliation (Dermatology, 1994, Yeogak-gak). In addition, a keratin exfoliation promoting substance is used to remove the melanin already produced. However, alpha-hydroxy acid complains of tingling and irritation due to low pH, so the use concentration and pH control are very important to alleviate side effects of the skin. In addition, it is possible to reduce skin side effects by attaching proteins, lipids, etc. to alpha hydroxy acids, or by adding side effect relievers, and by changing the structure, but the effects such as exfoliation and fine wrinkles are also reduced to minimize the side effects and maximum effects. It is still difficult to obtain.

Therefore, the present invention overcomes side effects and stability problems such as skin tingling such as alpha hydroxy acid (AHA) and trichloroacetic acid, which are known to date, have no toxicity and have good stability, and exfoliate more than conventional substances. It is an object of the present invention to provide an exfoliating accelerator having excellent effects.

[Means for solving the problem]

In order to achieve the above object, the present invention provides 5,15-diacetoxy-3-phenylacetoxy-14-oxolatiadiene-6 (17)-represented by the following general formulas (I) and (II), respectively. Keratin exfoliation promoter selected from epoxide, and 5,15-diacetoxy-3,7-dibenzoyloxy-14-oxoratiadiene.

[Formula I]

[Formula II]

The present invention also provides a pharmaceutical or cosmetics containing the above keratin exfoliation accelerator as an active ingredient.

The drug or cosmetic preferably contains 0.0001-10.0% by weight of a keratin exfoliation accelerator in dry weight.

The medicines or cosmetics include skin softeners, acne healing and prevention agents, wrinkle removers or whitening agents.

Hereinafter, the present invention will be described in detail.

The keratin exfoliation promoter of the general formulas (I) and (II) may be obtained by chemical synthesis or by extracting those present in various animals, plants, and microorganisms, and typically by extracting from Euphorbia lathyris L. You can get it. The compounds of formulas (I) and (II) extracted from the recipients are generally known as Euphorbia factors L1 and L2, respectively.

Euphorbia lathyris L. is a biennial herb and uses the seed of the deceased in the present invention. It is mentioned in the agreement that it is effective in water species, row hydrogen species, and the like, and a patent (JP08175954) relating to the whitening effect of the water extract is reported, but it is certainly different from the present invention. Are distinguished. Ingenol and Euphorbia factors L1-L8 have been identified in the existing component research literature of the recipients (J. Nat. Prod., 1999, 62, 76-79; Tetrahedror. Letters, 1971, 18, 1325-1329). The structural analysis data (NMR, MASS) of Euphoria factors L1 and L2 used in the present invention were confirmed by comparison with literature values.

Euphorbia factors L1 and L2 of the present invention are used in the plant, such as the inner water, but the present invention is not limited thereto.

In the present invention, Euphorbia factor L1 was purified from an organic solvent extract of Euphorbia lathyris L. containing a large amount of Euphorbia factors L1 and L2, or oil obtained by pressing. Purification of Euphorbia Factor L1 includes liquid-liquid extraction using various solvents such as purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, hexane, cyclohexane, petroleum ether, silica gel or active eggs. Purification methods such as column chromatography packed with various synthetic resins such as lumina and high performance liquid chromatography (HPLC) were used. In particular, it is not limited to the above purification method.

An example of the refining method will be described in more detail. First, a commercially available fastener is purchased as a herbal medicine, finely pulverized, and 5 to 20 volumes of water or 1 to 4 carbon anhydride or hydrous lower alcohol, Extract with ethyl acetate, hexane or chloroform by heating to 50-100 ° C. for 1-5 hours in an extractor equipped with a reflux condenser. After filtering with a filter cloth, the residue is further extracted one or more times in the same manner. The extracts are combined and concentrated under reduced pressure to give a dry extract. The dried Sucrose extract is suspended in water, and then the same volume of hexane is added, followed by liquid-liquid extraction to obtain an oily hexane fraction. Alternatively, a commercially available fastener is purchased and pulverized finely, and compressed and filtered at a temperature in the range of room temperature-250 ° C to obtain a fastener oil. Solvents such as ethanol, methanol, acetonitrile, purified water, etc., which are not mixed with oil, are mixed with each other in various ratios or added 0.2-10 times to the hexane fraction obtained by pressing or compressed oil, and then liquid-liquid extraction. Obtain the solvent fraction. Hexane was added to the obtained solvent fraction to form a precipitate, or the solvent fraction was concentrated under reduced pressure, and then hexane was added to obtain an insoluble hexane insoluble fraction. These precipitates and insoluble fractions were subjected to fractional column chromatography to separate Euphorbia factors L1 and L2. It was purified again by high performance liquid chromatography. The separated purified was confirmed by mass spectrometry and nuclear magnetic resonance spectra.

The present invention comprises the skin obtained by the above-mentioned Euphorbia factor L1, L2, including cosmetics, soaps, foam cleansing, body cleanser, shampoo, rinse, etc., including flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, peck and the like. It is added to hair cleansers and skin ointments. DETAILED DESCRIPTION OF THE INVENTION In more detail, the Euphorbia factors L1 and L2 are 0.0001% to 10% (W / W), preferably 0.001% to 5% (W / W), based on the dry weight of the cosmetics mentioned above, It is added to skin and hair cleansers, skin ointments. Below this concentration, it is difficult to expect a substantial exfoliation effect, and at the above concentration no further exfoliation effect is increased, and also affects the formulation and product stability.

The present invention will be described in more detail with the following experiments and examples. However, it should be noted that the present invention is not limited to the following examples.

Example 1

Purifier was purchased and 100 g of the pulverized product was put in 500 ml of 80% methanol solution, boiled for 3 hours in a reflux extractor equipped with a cooling condenser, filtered through a 300 mesh filter cloth, and the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble matters, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, suspended in 300 ml of purified water, and then 300 ml of hexane was added. Shake to obtain hexane lysate. This hexane lysate was chromatographed on a silica gel column to give 650 mg of a fraction containing the active ingredient. This was further purified by high performance liquid chromatography to separate 200 mg and 300 mg of Euphorbia factor L1 and L2, respectively.

Example 2

The water injector was purchased, and 100 g of the pulverized product was pressed at room temperature and filtered to obtain 35 mL of the oil in the inoculator. 35 ml of ethanol was added to the oil obtained above, and the ethanol fraction obtained by liquid-liquid extraction was chromatographed on a silica gel column to obtain 700 mg of a fraction containing the active ingredient. This was further purified by high performance liquid chromatography to separate 210 mg and 350 mg of Euphorbia factor L1 and L2, respectively.

Example 3

100 g of ground product was put in 500 ml of 100% ethanol solution, and it carried out by the same method as Example 1, and obtained 200 mg and 310 mg of Euphorbia factors L1 and L2, respectively.

Example 4

100 g of the ground water was crushed into 500 ml of chloroform solution, and the same method as in Example 1 was carried out to obtain 360 mg and 250 mg of Euphorbia factors L1 and L2, respectively.

Example 5

100 g of the fastener pulverized product was put into 500 ml of n-hexane solution, boiled for 3 hours in a reflux extractor equipped with a cooling condenser, filtered through a 300 mesh filter cloth, and the residue was extracted once more in the same manner. Combine each extract and use only the white at room temperature

After filtering by filter paper No. 2, the insoluble substance was removed, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and chromatographed on a silica gel column to obtain 800 mg of the fraction containing the active ingredient. It was purified again by high performance liquid chromatography to separate 380 mg and 280 mg of Euphorbia factor L1 and L2, respectively.

Example 6

100 ml of 80% ethanol was added to 35 ml of the oil obtained by the same method as in Example 2 using 100 g of the ground water, and the ethanol fraction obtained by liquid-liquid extraction was purified by chromatography on a silica gel column. 500 mg of the fraction containing the component was obtained. This was further purified by high performance liquid chromatography to separate 270 mg and 180 mg of Euphorbia factor L1 and L2, respectively.

Example 7

The inner water was purchased, and 100 g of the ground product was pressed at 150 ° C. and filtered to obtain 40 ml of the oil of the ground water. 200 ml of 90% acetonitrile was added to the oil obtained above, and the acetonitrile fraction obtained by liquid-liquid extraction was chromatographed on a silica gel column to obtain 520 mg of the fraction containing the active ingredient. This was further purified by high performance liquid chromatography to separate 280 and 190 mg of Euphorbia factor L1 and L2, respectively.

Example 8

The inner water was purchased, and 100 g of the ground product was pressed at 150 ° C. and filtered to obtain 40 ml of the oil of the ground water. 10 ml of 80% ethanol solution was added to the oil obtained above, hexane was added to the ethanol fraction obtained by liquid-liquid extraction, and the precipitate was washed with hexane to obtain 450 mg of hexane insoluble fraction. This was further purified by high performance liquid chromatography to separate Euphorbia Factor L1 and L2 270 mg and 160 mg, respectively.

Experimental Example 1

The exfoliation promoting effect was measured on the Euphorbia factors L1 and L2 obtained according to Examples 1 to 8 above. The test subjects were 30 healthy men aged 20 to 40 years. Before applying the sample, the color of the inside of the test subject's upper arm was measured with a chromameter (CR-200, Minolta, Japan), and 10% dihydroxy acetone (DHA) was added to the Hill Top chamber (manufactured by The Hill Top). About 0.4 mL was added and attached to the inner arm upper arm for 6 hours. After 24 hours, the color of the brown colored area was measured by DHA to compare the color difference with that before the sample application. Thereafter, while applying a sample dissolved in 5% ethanol twice a day, the degree of discoloration was measured by a chromameter every day to measure the time to return to the original skin color. Keratin peeling rate was calculated by the following formula.

* Keratin exfoliation rate (%) = (TT of blank-TT at sample application) / TT x 100 of blank

TT (Turnover Time): The time it takes for the old stratum corneum to be replaced by the new stratum corneum (in this experiment, the time it takes for the skin colored by DHA to fully revert).

TABLE 1

Comparison of exfoliation effect between conventional alpha hydroxy acid and Euphorbia factor L1, L2

Type of sample Turnover Time (days) Keratin exfoliation rate (%) Blank * 18.2 ± 3.3 - Control (ethanol) 17.4 ± 4.8 4.2 5% glycolic acid 14.4 ± 4.3 21.0 Lactic acid 5% 14.6 ± 4.0 19.8 Euphoria Factor L1 5% 11.2 ± 2.2 38.2% Euphoria Factor L2 5% 10.9 ± 1.5 40.3%

Blank *: No sample applied after staining with DHA

In Table 1, glycolic acid and lactic acid, which are known as excellent exfoliants, showed about 20% exfoliation effect when the 5% concentration was used, whereas the euphoria factors L1 and L2 of the same concentration were mentioned above. Compared to the two alpha hydroxy acids, the exfoliation effect was about 2 times 38.2% and 40.3%.

[Table 2] The exfoliation effect of Euphorbia factor L1, L2 according to the concentration

Type of sample Turnover Time (days) Keratin exfoliation rate (%) Blank 18.2 ± 3.3 - Control (ethanol) 17.4 ± 4.8 4.2 Euphoria Factor L1 10% 9.4 ± 1.5 48.3 Euphoria Factor L1 5% 11.2 ± 2.2 38.2 Euphoria Factor L1 2% 14.5 ± 2.2 20.8 Euphoria Factor L1 1% 14.8 ± 3.0 18.8 Euphoria Factor L1 0.1% 16.3 ± 4.6 10.2 Euphoria Factor L1 0.01% 16.9 ± 5.8 7.3 Euphoria Factor L2 10% 8.4 ± 1.3 53.6 Euphoria Factor L2 5% 10.9 ± 1.5 40.3 Euphoria Factor L2 2% 13.1 ± 2.4 28.1 Euphoria Factor L2 1% 14.2 ± 3.5 22.0 Euphoria Factor L2 0.1% 15.8 ± 5.0 13.3 Euphoria Factor L2 0.01% 16.8 ± 5.8 7.7

In the above results, when the concentration of Euphorbia factor L1 and L2 was 10%, the exfoliation effect was 48.3%, 53.6%, and less than 10% at 0.01% concentration.

Experimental Example 2

As described above, the skin test for stinging was measured with the Euphorbia factors L1 and L2 having excellent exfoliation effect as follows.

The test subjects were 30 healthy women aged 20 to 35 years. First of all, after washing the face to remove previously secreted sebum and dust, it was adapted for 15 minutes in a constant temperature and humidity room (27 ℃, 70% relative humidity). 0.4 mL of the sample dissolved in ethanol was soaked in the nonwoven fabric and removed after 20 seconds after attaching the nonwoven fabric around the nose and cheeks. After removing the nonwoven fabric, the degree of tingling was measured by subjective judgment according to the following criteria for each time interval of 10 seconds, 150 seconds, 300 seconds, and 480 seconds.

* Based on sting score

0: There is no feeling. 1: I sting slightly.

2: I am quite stinging. 3: I feel badly tingling.

[Table 3] criteria for stinging degree

Sting score Judgment 0.0-0.3 No stinging effect 0.4-1.0 Slightly stingy 1.1-2.0 Stinging 2.1-3.0 Causes severe stinging

[Table 4] Score of degree of stinging according to sample application

Type of sample Time in seconds Hourly score Average score ethanol 10 0.1 0.13 150 0.2 300 0.1 480 0.1 5% glycolic acid 10 1.9 2.08 150 2.5 300 2.4 480 1.5 Lactic acid 5% 10 1.8 1.95 150 2.2 300 2.2 480 1.6 Euphoria Factor L1 5% 10 0.4 0.50 150 0.8 300 0.5 480 0.3 Euphoria Factor L2 5% 10 0.3 0.48 150 0.8 300 0.5 480 0.3

In Table 4, Euphorbia factors L1 and L2 showed a low degree of stinging compared to glycolic acid and lactic acid at the same concentration.

As described above, the preparation example was carried out with the Euphorbia factors L1 and L2 having excellent keratin exfoliation effect and low stinging compared with the conventional alpha hydroxy acid. However, it should be noted that the present invention is not limited only to the following preparation examples.

Preparation Example 1-2 and Comparative Example 1

Examples of prescription ointments for external skin containing Euphorbia factors L1 and L2 are as follows.

Composition Preparation Example (wt%) Comparative Example 1 One 2 -Active ingredient Euphoria factor L1 Euphoria factor L2-Diethyl sebacate-Brazing-Polyoxyethylene oleyl ether phosphate-Sodium benzoate-Vaseline  10-8 5 6 Proper Rest  -10 8 5 6 appropriate amount remaining  --8 5 6 Proper Rest

Preparation Example 3-4 and Comparative Example 2

Prescription examples of creams in the cosmetics containing Euphoria factors L1 and L2 are as follows.

Composition Preparation Example (wt%) Comparative Example 2 3 4 -Active Ingredients Euphoria Factor L1 Euphoria Factor L2-Stearic Acid-Cetanol-Potassium Hydroxide-Glycerine-Propylene Glycol-Preservative-Fragrance-Purified Water  1.0-15.0 1.0 0.7 5.0 3.0 appropriate quantity remaining  -1.0 15.0 1.0 0.7 5.0 3.0 appropriate quantity remaining  --15.0 1.0 0.7 5.0 3.0 appropriate quantity remaining

Preparation Examples 5-6 and Comparative Example 3

Prescription examples of flexible lotion in cosmetics containing Euphoria factors L1 and L2 are as follows.

Composition Preparation Example (wt%) Comparative Example 3 5 6 -Active ingredient Euphorbia factor L1 Euphorbia factor L2-Ethanol-Polyoxyethylene sorbitan polylaurate-Methyl paraoxybenzoic acid-Glycerin-1,3-butylene glycol-Fragrance-Colorant-Purified water  0.2-10.0 1.0 0.2 5.0 6.0 Proper quantity remainder  -0.2 10.0 1.0 0.2 5.0 6.0 appropriate quantity remaining  --10.0 1.0 0.2 5.0 6.0 Proper quantity remaining

Preparation Examples 7-8 and Comparative Example 4

Prescription examples of nutritional lotion among cosmetics containing Euphoria factors L1 and L2 are as follows.

Composition Preparation Example (wt%) Comparative Example 4 7 8 -Active ingredient Euphorbia factor L1 Euphorbia factor L2-Waselin-Sesquioleate sorbitan-Polyoxyethylene oleyl ethyl-Paraoxybenzoic acid methyl-Propylene glycol-Ethanol-Carboxy vinyl polymer-Potassium hydroxide-Pigment-Flavor-Purified water  0.1-2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 proper quantity remaining  -0.1 2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 proper quantity remaining  --2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 proper quantity remaining

Preparation Example 9-10 and Comparative Example 5

Prescription examples of packs among cosmetics containing Euphoria factors L1 and L2 are as follows.

Composition Preparation Example (wt%) Comparative Example 5 9 10 -Active Ingredients Euphoria Factor L1 Euphoria Factor L2-Glycerine-Propylene Glycol-Polyvinyl Alcohol-Ethanol-Polyoxyethylene Oleethyl-Paraoxybenzoic Acid Methyl-Pigment-Fragrance-Purified Water  2.0-5.0 4.0 15.0 8.0 1.0 0.2 Proper quantity remainder  -2.0 5.0 4.0 15.0 8.0 1.0 0.2 Proper quantity remaining  --5.0 4.0 15.0 8.0 1.0 0.2 Proper quantity remaining

Preparation Examples 11-12 and Comparative Example 6

Prescription examples of essences in the cosmetics containing Euphorbia factors L1 and L2 are as follows.

Composition Preparation Example (wt%) Comparative Example 6 11 12 -Active Ingredients Euphoria Factor L1 Euphoria Factor L2-Propylene Glycol-Glycerine-Sodium Hyaluronate (1%)-Ethanol-Polyoxyethylene Cured Castor Oil-Paraoxybenzoic Acid Methyl-Flavor-Purified Water  5-10.0 10.0 5.0 5.0 1.0 0.1 Quantity Rest  -5 10.0 10.0 5.0 5.0 1.0 0.1 Quantity Rest  --10.0 10.0 5.0 5.0 1.0 0.1 Quantity Rest

Experimental Example 3

Keratin peeling effect and stinging test were carried out for the above production example and the results are shown in [Table 5].

Table 5 Determination of keratin exfoliation and stinging of Euphoria factor L1 and L2 containing formulations

Production Example Keratin exfoliation rate (%) Sting average score One 50.1 0.62 2 53.0 0.68 3 18.0 0.23 4 24.1 0.31 5 14.3 0.20 6 20.1 0.18 7 10.2 0.18 8 16.0 0.17 9 21.8 0.31 10 26.3 0.26 11 40.7 0.33 12 41.6 0.30

As can be seen in Table 5, the preparation examples according to the present invention show the exfoliation effect in all cases containing Euphorbia factor L1 and L2, and the degree of stinging was also low.

5,15-Diacetoxy-3-phenylacetoxy-14-oxoratyadiene-6 (17) -epoxide (Eupovia factor L1), and 5,15-diacetoxy, which are keratin exfoliation promoters of the present invention -3,7-dibenzoyloxy-14-oxoratyadiene (Eupovia factor L2) has been demonstrated to be superior to conventional alpha hydroxy acids in human exfoliation and skin tingling experiments. It is a material with excellent stability and safety and excellent exfoliation effect.

Claims (4)

5,15-diacetoxy-3-phenylacetoxy-14-oxolatiadiene-6 (17) -epoxide represented by the following general formulas (I) and (II), respectively, and 5,15-diacene A keratin exfoliation promoter selected from oxy-3,7-dibenzoyloxy-14-oxoratiadiene.

[Formula I]

[Formula II] Cosmetics containing the keratin exfoliation promoter of Claim 1 as an active ingredient. 3. A cosmetic according to claim 2, wherein said keratin exfoliation accelerator is contained 0.0001-10.0 wt% in dry weight. The cosmetic according to claim 2, wherein the medicine or cosmetic is a skin softening agent, an acne healing and preventing agent, a wrinkle remover or a whitening agent.