KR100558751B1 - Cosmetic composition for skin moisturizing - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing the skin, and more particularly, contains a subtilsin protease, Astragalus membranaceus, Astragalus mongholicus, Zizyphus jujuba, and hyaluronic acid. It relates to a skin moisturizing cosmetic composition that improves roughness, lack of elasticity.

Subtilisin * Astragalus * Jujube * Hyaluronic Acid * Pull * Roughness * Moisturizing

Description

Cosmetic composition for skin moisturizing {Cosmetic composition for skin miosture}

The present invention relates to a cosmetic composition for moisturizing the skin, and more particularly, contains a subtilsin protease, Astragalus membranaceus, Astragalus mongholicus, jujube (Zizyphus jujuba) and hyaluronic acid (hyaluronic acid) It relates to a skin moisturizing cosmetic composition that improves roughness, lack of elasticity.

The skin is divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside, and it protects the organs of the body from temperature and humidity changes, ultraviolet rays and other physical and chemical external stimuli. In particular, the epidermis plays an important role in preventing evaporation of moisture inside the human body. The epidermis is divided into the stratum corneum, granule layer, pore layer, and basal layer in order from the outside, and the cells of the stratum corneum act as bricks, and the intercellular lipids between the stratum corneum act as the mortar to form the skin barrier ( J. Invest.Drmatol. 80 (Suppl.), 44-49. 1983. In addition, the keratinocytes of healthy people have high concentrations of Natural Moisturing Factor (NMF) to help the skin retain moisture. For example, because amino acids such as amino acids are water-soluble, they can effectively combine with moisture Inhibit drying (J. Invest. Dermatol. 54, 24-31, 1970).

However, these days, the artificial temperature control of air conditioning and heating according to the change of environment and life patterns, the skin stress caused by various stresses and environmental pollution that occur in social life, the frequent face wash due to makeup habits and the increase of age Due to various causes such as aging of the skin, the moisture of the stratum corneum decreases, the skin becomes dry, the surface becomes rough, and the skin becomes crumbly and loses luster, and the appearance of dullness occurs.

Conventionally, a method of increasing moisture retention in the stratum corneum by using a humectant having a property of absorbing moisture or an occlusive moisturizer that prevents evaporation of moisture as a moisturizing agent is used. Examples of the humectants include glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, and 2-pyrrolidone-5-sodium carbonate. There is a drawback to feeling. In addition, as a closed moisturizer, lipid components such as ceramide, essential fatty acids and lipid complexes were used (J. Invest. Dermatol. (5), 731-740, 1994). There are disadvantages inadequate for the manufacture of the tops.

However, in recent years, the claim that differentiation of the stratum corneum is important to prevent drying of the skin moisture, the differentiation process that develops from the basal keratinocytes to the outermost stratum corneum cells should be performed normally as a role as a skin moisture retention barrier It turns out that you can. That is, during the process of keratinization, the cells produce natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes firm and flexible, thus functioning as a barrier. Physiologically, the tendency of the skin to dry with age increases, which can be interpreted as increasing the dropout time of the stratum corneum, decreasing the lipid synthesis ability of epidermal cells, and decreasing the amount of moisturizing factors and lipids in the stratum corneum. . Thus, by accelerating the differentiation of keratinocytes, a new approach has been made that can induce a strengthening of the skin barrier and enhance skin moisture retention and protection from the external environment.

Accordingly, the present inventors studied a cosmetic composition having excellent skin moisturizing effect and no stickiness or slack when applied to the skin to improve skin pulling, roughness, lack of glossiness, etc. The cosmetics containing Astragalus, Jujube, and Hyaluronic Acid were prepared, and when applied to human skin, it was confirmed that the skin moisturizing effect was actually excellent. This moisturizing effect promotes the differentiation of keratinocytes and induces the strengthening of the skin barrier. It was found that this was achieved by improving skin moisture retention function, and completed the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition for moisturizing the skin which is excellent in moisturizing effect on the skin to improve skin pulling, roughness and lack of elasticity.

In order to achieve the above object, the skin moisturizing cosmetic composition according to the present invention is characterized in that it contains a subtilisin protease, Astragalus, jujube, and hyaluronic acid. More preferably, the cosmetic composition for moisturizing the skin according to the present invention is 0.001 to 10% by weight of the subtilisin protease, 0.001 to 20% by weight of Astragalus extract, 0.001 to 20% by weight of jujube extract, and 0.000001 to hyaluronic acid. It is characterized by containing 10% by weight.

Hereinafter, the present invention will be described in more detail.

Subtilsin protease used in the present invention is an alkaline protease from Bacillus licheniformis, and has an effect of removing dead skin cells. Exfocellia, a commercial product of the subtilisin protease, is known to have a keratinizing effect stably in various formulations as a protease stabilizing material.

In the present invention, the above-mentioned exfocellia is used as a subtylsin protease, and the exfocellia used in the present invention is used in an amount of 0.001 to 10% by weight based on the total weight of the composition. If it is less than 0.001% by weight, the effect on the skin is weak, and if it exceeds 10% by weight, the skin is very sticky and not suitable as a cosmetic.

Astragalus extract used in the present invention is saponin of the astragalalosides (astragalosides) and polysaccharides of the astragalalan (astragalan), kaempferol, quercetin (quercetin), isorhamnetin (isorhamnetin), calicosin (calycosin) ), Flavonoids such as formononetin, rhamnocitrin, kumatakenin, amino acids such as asparagine, glutamic acid, proline, β-aminobutyric acid, arginine, alanine, etc. It promotes the regeneration of skin tissues, enhances immune function, enhances defense against external stimuli, and promotes blood circulation to smooth the nutrition supply to the skin.

Astragalus extract used in the present invention was used to extract the root of Astragalus membranaceus or Astragalus mongholicus using a hydrophilic solvent 1,3-butylene glycol and water, the amount of 0.001 ~ 20% by weight based on the total weight of the composition do. This is because the effect on the skin is less than 0.001% by weight, and if it is more than 20% by weight, there is a possibility of precipitation in the formulation, which is not suitable as a cosmetic.

Jujube extract used in the present invention is rich in vitamins A, B2, C, amino acids, sugars, proteins, β-carotene and the like, and triterpene glucosides such as sterols, coumarins, flavonoids and oleanolic acid and ursolic acid. It contains a compound of anti-inflammatory, moisturizing, soothing, wrinkle relief in the skin.

Jujube extract used in the present invention uses a component extracted from the fruit of Zizyphus jujuba using a hydrophilic solvent 1.3-butylene glycol and water, the preferred content thereof is 0.001 ~ 20% by weight. This is because the effect on the skin is less than 0.001% by weight, and the skin safety problem may occur above 20% by weight, so it is not suitable as a cosmetic.

Hyaluronic acid used in the present invention is a natural polymer obtained by culturing microorganisms, which is in the form of free acid or salt, is a kind of mucopolysaccharide, which is present in the connective tissue of the human dermal layer to maintain moisture. It is an ingredient that plays an important role in. The preferred content of hyaluronic acid used in the present invention is 0.000001 to 10% by weight. If it is less than 0.000001% by weight, the effect on the skin is weak, and when it exceeds 10% by weight, it is difficult to dissolve completely in water and it is not suitable as a cosmetic since it has a sticky feeling.

Hereinafter, although an Example and a test example are given and this invention is demonstrated more concretely, this invention is not limited only to these examples.

[Test Example 1] keratinocyte differentiation promoting effect

Human keratinocytes were placed in a culture flask, cultured and grown to about 80%, and the samples of Table 1 were each added at a concentration of 5 ppm and treated for 4 days, followed by urea or sodium dodecyl sulfate (SDS). The denatured protein was removed by mixing the same denaturant and reducing agent such as β-mercaptoethanol. Then, the peptide concentration was analyzed by measuring the absorbance at 310 nm of the remaining stratum corneum, and the results are shown in Table 1 by comparing the increase by each sample based on distilled water as a negative control (0%).

matter Keratinocyte differentiation promoting effect Subtylcin 75% Astragalus 61% Jujube 53% Hyaluronic acid 38% Distilled Water (Negative Control) 0%

As can be seen from the above results, it was confirmed that the keratinocyte differentiation promoting effect of the subtilisin protease, Astragalus, jujube, hyaluronic acid material used in the present invention is very excellent.

[Examples 1-3 and Comparative Examples 1-3] Preparation of Cosmetics for Skin Moisturizing

After dissolving the water parts up to 1-8 and the alcohol parts up to 9-12 in each of the contents shown in Table 2 at room temperature, the alcohol parts were gradually added to the aqueous phase while solubilizing with stirring. Next, after the addition of the alcohol part was completed, stirring was continued for about 3 to 5 minutes, followed by vacuum degassing to complete the preparation of the cosmetics of Examples 1 to 3 and Comparative Examples 1 to 3.

Component (content; wt%) Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 1. Purified water to 100 to 100 to 100 to 100 to 100 to 100 2. Glycerin 3 3 3 3 3 3 3. Carbomer 0.2 0.2 0.2 0.2 0.2 0.2 4. Triethanolamine 0.18 0.18 0.18 0.18 0.18 0.18 5. Subtylsin Protease 0.1 0.2 0.2 - 0.1 - 6. Astragalus Extract One 2 2 - One - 7. Jujube extract One One 2 - - One 8. Hyaluronic Acid One One 2 - - One 9. Ethanol 3 3 3 3 3 3 10. Polyoxyethylene Hydrogenated Castor Oil 0.3 0.3 0.3 0.3 0.3 0.3 11. Incense Quantity Quantity Quantity Quantity Quantity Quantity 12. Preservative Quantity Quantity Quantity Quantity Quantity Quantity

Test Example 2 Skin Barrier Function Restoration Effect

After repeatedly applying acetone to the skin of hairless mice, the barrier function was impaired. The epidermal water loss (TEWL) was measured by servomed evaporimeter EP1 and measured at 4.0 g / ㎡ / h. When reaching, each of the samples containing the composition of Table 2 was applied to an area of 5 cm 2, and the barrier function was evaluated by measuring the amount of epidermal moisture loss after 1 hour, 2 hours, 4 hours, and 8 hours. The extent of recovery was evaluated. At this time, a known lipid mixture (2: 1: 1 mixture of ceramide: cholesterol: fatty acid) was used as a positive control. The results are shown in Table 3 below comparing the changes over time based on the amount of epidermal moisture loss in the initial damaged barrier state (100%).

Epidermal moisture loss (%) sample 1 hour later 2 hours later 4 hours later 8 hours later Example 1 63 55 40 27 Example 2 57 53 35 23 Example 3 52 43 35 22 Comparative Example 1 85 87 77 73 Comparative Example 2 63 52 47 40 Comparative Example 3 75 60 47 37 Geological Mixture (Yangseong Control) 64 44 38 27

As shown in the above results, it was confirmed that the skin barrier function recovery effect of Examples 1 to 3 according to the present invention is very excellent compared to Comparative Examples 1 to 3.

[Test Example 3] skin moisture content increase effect

For each cosmetic prepared in the composition of Table 2 was tested the effect of improving the skin moisturizing power. For 20 people in their 20s and 30s without skin diseases, 20 people per group were divided into 7 sets, and each group was given the same Examples 1 to 3 and Comparative Examples 1 to 3 and a positive control group as in Test Example 2, Twice daily for 1 month on face and forearm. Before starting the application, measure the conductivity of the skin using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 degrees, 40% humidity), and after 1 week, 2 weeks and 4 weeks And skin conductivity two weeks after application discontinuation (6 weeks) and the rate of increase was evaluated. The results are shown in Table 4 below.

Skin conductivity increase rate (%) sample 1 week later after 2 weeks 4 weeks later 6 weeks later Example 1 57 62 58 43 Example 2 59 63 69 57 Example 3 60 66 72 60 Comparative Example 1 11 15 18 9 Comparative Example 2 42 43 47 25 Comparative Example 3 43 47 51 37 Geological Mixture (Yangseong Control) 62 65 63 45

In general, the skin conductivity is proportional to the skin moisture content, it was confirmed that the effect of increasing the skin moisture content of Examples 1 to 3 according to the present invention in the results of Table 4. In addition, in the case of Examples 2 and 3, even after two weeks of discontinuation (after six weeks in total), the moisture content was maintained higher than other examples, and thus it was found to have a long-term moisturizing effect.

In addition, subjects were subjected to subjective efficacy evaluation through a questionnaire survey on skin pulling, roughness and elasticity after using Examples 1-3 and Comparative Examples 1-3. The questionnaire survey was to express the satisfaction level of the product for each item of skin pull, roughness, elasticity change by 1-5 points (the higher the number, the higher the satisfaction), and the results are shown in Table 5 below.

sample Satisfaction (Average Score) Improved skin pull Skin Roughness Improvement Increased skin elasticity Example 1 3.5 3.8 3.3 Example 2 4.3 4.6 3.8 Example 3 4.2 4.8 4.2 Comparative Example 1 1.6 1.3 2.2 Comparative Example 2 1.4 2.1 2.4 Comparative Example 3 2.7 3.4 2.7

From the results of Table 5, it can be seen that in the case of Examples 1 to 3 of the present invention, the effect of improving the skin pulling, roughness and gloss is superior to Comparative Examples 1 to 3.

As described above, the cosmetic composition according to the present invention contains subtylcin protease, Astragalus extract, jujube extract, and hyaluronic acid as an active ingredient to promote the differentiation of keratinocytes of the skin and restore the skin barrier function, It has an effect of relieving pulling and roughness and increasing elasticity, and thus may be usefully used as a skin moisturizing cosmetic.

Claims (6)

A skin moisturizing cosmetic composition comprising subtilisin protease, astragalus extract, jujube extract and hyaluronic acid as an active ingredient. The method according to claim 1, wherein the active ingredient contains 0.001 to 10% by weight of subtylcin protease, 0.001 to 20% by weight of Astragalus extract, 0.001 to 20% by weight of jujube extract and 0.000001 to 10% by weight of hyaluronic acid. Cosmetic composition for skin moisturizing, characterized in that. The cosmetic composition according to claim 1, wherein the Astragalus extract is an ingredient extracted from the root of Astragalus membranaceus or Astragalus mongholicus . The cosmetic composition according to claim 1, wherein the jujube extract is an ingredient extracted from the fruit of Zizyphus jujuba . The cosmetic composition according to claim 1, wherein the hyaluronic acid is a natural polymer component obtained by culturing microorganisms and is in the form of a free acid or salt. A skin composition for enhancing skin elasticity, comprising a subtilisin protease, astragalus extract, jujube extract, and hyaluronic acid.