KR100534074B1 - Cosmetic composition containing grain extract and fermented grain phellinus linteus extract, and cosmetics containing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of a cereal mixture comprising sorghum, black beans, white beans, red beans, and black sesame, and an extract of brown rice or juvenile germ mycelium, wherein the cosmetic composition according to the present invention is very excellent. It has free radical scavenging ability, tyrosinase activity inhibiting activity, antioxidant effect and sunscreen effect, and thus can be usefully used as a cosmetic composition for the purpose of preventing skin aging, improving wrinkles, skin whitening, skin moisturizing or sunscreen. In addition, the cosmetic composition according to the present invention can provide a more improved skin aging, wrinkle improvement, skin whitening effect, such as when using the extract of grain mixture or grain germination mushroom extract alone.

Description

Cosmetic composition containing grain extract and grain germination mushroom extract and cosmetics containing the same {COSMETIC COMPOSITION CONTAINING GRAIN EXTRACT AND FERMENTED GRAIN PHELLINUS LINTEUS EXTRACT, AND COSMETICS CONTAINING THE SAME}

The present invention has excellent free radical scavenging ability, tyrosinase activity inhibiting activity, antioxidant effect and sunscreen effect, and thus is usefully used as a cosmetic composition for the purpose of preventing skin aging, wrinkle improvement, skin whitening, skin moisturizing or sun protection. It relates to a composition that can be.

Phellinus linteus is a rare mud mushroom that grows rarely in the old tree of mulberry. It is extremely rare to grow naturally in nature, and it is difficult to collect the situation fruit body with the decrease of mulberry. Is almost in practical use (JP 1996-013174, 2000-001853, Japan 0180097, etc.).

In addition, the situation mushroom has been reported to have the strongest anti-tumority among the mushrooms, it was also found to be excellent in the bactericidal and bactericidal effect against pathogenic bacteria causing various skin diseases.

In addition, the situation mushroom has been widely used in various fields, in particular, cosmetics for skin improvement. For example, Patent Application No. 1996-57166 discloses a cosmetic composition having an anti-aging effect of skin containing an extract obtained from a mycelium of a situation or its culture solution; Patent application No. 1996-69542 discloses a composition excellent in preventing and improving skin damage, immuno-enhancing effect, whitening effect, antioxidant effect, etc. containing a situation mushroom extract; Patent Application No. 1999-11685 discloses antibacterial and antibacterial cosmetic soaps containing situational ingredients such as situational extracts, situational powders, situational mycelium, and situational cultures; Patent application 2001-8433 describes a composition having excellent skin moisturizing power, elasticity, stability and whitening effect containing niobium containing natural situation mushroom extract.

However, although the cosmetic compositions containing the conventional situation mushroom component has various skin improvement effects such as anti-wrinkle, skin aging, skin elasticity improvement, and antioxidant effect, their effects are limited, and thus, other than the situation mushroom component Containing the component of, efforts to further improve the skin improvement effect has been carried out in the art. For example, Patent Application No. 2001-24063 discloses a composition that further improves the anti-wrinkle effect, collagen fiber biosynthesis effect, and skin elasticity enhancing effect by containing a situation mushroom extract and a grape fermentation extract.

Therefore, the present inventors also conducted a number of studies to find a method for further improving the various skin improvement effects such as skin aging, wrinkle improvement, skin whitening, skin elasticity improvement, skin moisturizing effect obtained by the situation mushroom extract, sorghum The skin-improving effect of the extract comprising a mixture of grains including black beans, white beans, red beans, and black sesame seeds and brown rice or juvenile sprouted mushroom extract (hereinafter, referred to as grain germinated mushroom extract) The present invention was found to complete the present invention.

Therefore, an object of the present invention has excellent free radical scavenging ability, tyrosinase activity inhibiting ability, antioxidant effect and ultraviolet ray blocking effect, thus cosmetics for the purpose of skin improvement effect such as skin aging prevention, wrinkle improvement, skin whitening, skin moisturizing It is to provide a composition that can be usefully used as a composition.

In order to achieve the above object, the cosmetic composition according to the present invention is characterized in that it contains an extract of a cereal mixture including sorghum, black beans, white beans, red beans, and black sesame, and grain germination situation mushroom extract.

Hereinafter, the present invention will be described in more detail.

The cosmetic composition according to the present invention, in order to further enhance the skin improvement effect, such as skin aging prevention, wrinkle improvement, skin whitening, skin moisturizing or UV blocking effect of the situation mushroom, sorghum, black beans, white baektae, red beans, and black sesame seeds It is characterized by containing an extract of the cereal mixture comprising. In particular, the cosmetic composition according to the present invention by providing both the extract of the grain mixture and the grain germination situation mushroom extract, it can provide a significantly increased skin improvement effect.

The brown rice used to obtain the grain germination situation mushroom extract included in the composition according to the present invention is not particularly limited in its form, but can be used in the state of peeling only the rice husk and peeling from the rice. Brown rice contains many physiologically active ingredients such as vitamins B1, B2, E, sugars, proteins, fats, minerals, linolenic acid, gamma-orizanol, and inositol (Song Bo-hyun, Korean Journal of Agricultural Chemistry, 30, 1987).

In addition, sorghum (Sorghum bicolor Moench) contained in the grain mixture according to the present invention contains a lot of vitamin B1, B2, phosphorus, iron, calcium and the like, as a food raw material for the purpose of treating intestinal action and constipation As a widely used substance, the composition according to the present invention can exhibit excellent skin improvement effect by containing the extract ingredients of sorghum.

In addition, the black soybean and white milk contained in the grain mixture according to the present invention are rich in linoleic acid and linolenic acid, thereby inhibiting an increase in cholesterol or fatty acids in the blood, thereby improving blood circulation and increasing the content of aspartic acid. It is known to help the activity of the liver and kidneys. Therefore, the composition according to the present invention can exhibit an excellent skin improvement effect by containing black beans and white milk having this function.

In addition, the red beans contained in the grain mixture according to the present invention contain special components such as saponin and choline pigment, have a diuretic and obesity inhibitory effect, and are known to play a role in relieving mental stress. On the other hand, the cosmetic composition according to the present invention was found to have an improved skin improvement effect, by containing the extract component of the red bean.

In addition, Yulmu used to obtain the grain germination situation mushroom extract included in the composition according to the present invention is used as a therapeutic agent for diuresis, tonic, analgesic, anti-inflammatory and is also used as a medicine for adult diseases, nutrition of the part removed The ingredient is known to contain 13.8% protein, 5.1% lipid, 67.7% sugar, 0.7% fiber, 1.6% ash, and some minerals and vitamins.

In addition, the black sesame seed contained in the grain mixture according to the present invention contains a high amount of anti-hyperlipidemic and antioxidant activities such as lecithin, choline, linolenic acid, sesamine, and is known to be a food that prevents constipation and has excellent anti-inflammatory action. have. In the present invention, it was found that by containing the extract component of the black sesame, it can provide a further improved skin improvement effect.

On the other hand, the method of extracting the grain mixture according to the present invention is not particularly limited, but may preferably be obtained by performing organic solvent extraction or hydrothermal extraction.

The method for obtaining an extract of the grain mixture by organic solvent extraction is not particularly limited on a weight basis, and for example, the mixing ratio of sorghum, black beans, white beans, red beans and black sesame seeds (0.1 -5.0): (0.1-8.0): (0.1-5.0): (0.1-8.0): (0.1-8.0) ratio, Preferably it mixes in the ratio of 1: 2: 1: 1: 2, and said grains The mixture was finely ground with a grinder, and then 0.5 kg of ethanol, methanol, isopropyl alcohol and the like were added to 1 kg of the dry mixture obtained by filtration through a 50-150 mesh filter net. Grain is extracted by heating at 50-95 ° C for 30 minutes to 12 hours or more. The extract may be concentrated under reduced pressure according to a conventional method, and then lyophilized to obtain a grain extract.

As another method for obtaining an extract of the grain mixture by the organic solvent extract, 0.5 to 10 times of water is added to 1 kg of the pulverized mixture of the dried product obtained in the same manner as the organic solvent extraction method, and then 100 ° C. After heating at 30 minutes to 12 hours or more at, the extract is concentrated under reduced pressure according to a conventional method, and then lyophilized to obtain an extract of the cereal mixture.

On the other hand, grain germination situation mushroom mycelium according to the present invention can be prepared by the following method. In other words, the situation mushrooms that have been widely used are already cultured in a petri dish (produced by the Rural Development Administration; strain name IJ209) in a petri dish, and cultured with a culture inoculum. When about 70% of the mycelium of the expanded mushrooms grown on a flat plate is grown, it is used as a preliminary culture for culturing with liquid spawn. Situation mushroom mycelium pieces inoculated in a liquid medium of 3.5% black sugar, 3.1% starch syrup, 0.3% soy flour, and then incubated for 150rpm / 28 ℃ / 8 days to produce a whole culture inoculum. On the other hand, the medium production of the situation mushroom is to adjust the moisture to 65% for brown rice or yulmu, 50% filled in a 1000ml plastic container, sterilized for 25 minutes at 121 ℃, then cooled and used as a culture medium. In this culture medium, the above-mentioned mushroom or mycelium mushroom mycelium can be cultured to obtain the grain germinating mushroom or mushroom mushroom mycelium.

That is, inoculate the pre-cultivated situation mushroom mycelium in a sterile state, and incubate by laying the medium or obliquely in order to increase the contact surface with air, and at this time, by growing a large amount of mycelium on the surface, it is collected as a situation mushroom mycelium. use. At this time, the culture conditions are incubated for 100 days while maintaining a humidity of 70-80%, temperature 28 ℃. After completion of the cultivation, the diseased mushrooms were removed in a sterile chamber, cut into 2-3 cm thickness, and placed in an intact container, followed by secondary culture for 5 days in a culture chamber of temperature 30 ° C., humidity 90%, carbon dioxide 0.3%, and precursor 500Lux. Prepare mycelium and internal mushrooms. The molded mushroom shaped article thus formed was put in a hot air dryer and hot-air dried at 65 ° C. for 48 hours, and used as a brown rice (yield) situation mushroom mycelium and a situation mushroom (grain cereal mushroom) of the present invention.

On the other hand, the extract of the grain germination situation mushroom or situation mushroom mycelium contained in the cosmetic composition according to the present invention, 1 to 3 times the amount of distilled water is added to the situation mushroom or situation mushroom mycelium, 1 to 3 hours at 105 ℃ to 115 ℃ After repeating the boiling water extraction process for 2 to 3 times, concentrated under reduced pressure to obtain a situation mushroom extract containing a polysaccharide component in the extract, and then the resulting extract was filtered through 260 mesh, 700 mmHg at 60 ℃ Concentration under reduced pressure can be obtained through the method of 25 brix.

On the other hand, the extraction method of the grain mixture described above and the extraction conditions of the situation mushroom or mushroom mushroom mycelium are general conditions used for extracting the physiologically active components they contain, so long as the physiological activity of the extraction component is maintained Various modifications can be made to the conditions.

In addition, the use form of the grain germination situation mushroom which is a raw material used to obtain the grain germination situation mushroom extract contained in the cosmetic composition according to the present invention is not particularly limited, for example, grain germination situation mushroom, grain germination situation Mushroom powder, grain germination mushroom mycelium, grain germination mushroom culture, preferably grain germination mushroom mycelium.

On the other hand, the composition according to the present invention, based on the total dry weight of the composition, the grain extract obtained by extracting a cereal mixture containing sorghum, black beans, white beans, red beans, black sesame, 1 to 99% by weight, and grain germination situation It can contain 99 to 1% by weight of the mushroom extract.

The cosmetic composition according to the present invention has a synergistic effect on the skin improvement effect such as skin whitening effect by using the two in combination, as compared to the case of using only one component of the mixture of the grain mixture and the mixture of grain germination situation mushroom extract Can be represented.

In addition, the composition according to the invention can function as a free radical scavenger, antioxidant, anti-inflammatory or sunscreen, thus protecting the organs of the body from changes in temperature or humidity and external environmental stimuli such as ultraviolet rays or various pathogenic microorganisms. It can be usefully used to prevent the degradation of skin function and the promotion of aging of the skin caused by various harmful components resulting from continuous physical or chemical stimulation or metabolism from the outside.

In addition, the composition according to the present invention, in addition to the above-described functions, known functions of the situation mushroom, such as anti-cancer effect, anti-viral effect of acne treatment effect; And the above-mentioned functions of each grain can be exhibited without inhibiting the skin improvement effect.

The cosmetic composition according to the present invention having the above-mentioned functions is formulated into various formulations such as skin, lotion such as lotion, nourishing lotion, massage cream, nourishing cream such as milk lotion, massage cream, pack such as beauty pack, cosmetic soap, etc. It may be formulated, but is not limited to such formulations. The materials added to prepare such formulations and their contents are obviously known in the art.

In particular the composition according to the invention may be formulated as a cosmetic soap. Currently, cosmetic soap has been developed with a focus on washing power, deodorizing power, skin protection, and skin cleanness, and in order to exert various physiological functions, various additives are added in small amounts, but most additives are not natural substances. Subsequently, it not only causes skin problems, but also has a disadvantage that it may cause a weak effect on the human body. Ordinary soaps are alkali metal soaps alone or in combination with fatty acids obtained from vegetable oils such as palm oil, sugar oil, soybean oil, olive oil, anti-coated oil, and animal fats and oils such as tallow, pork, sunny and fish oils alone, or a mixture thereof. It is prepared by neutralizing with alkali metal salts, and thus, in the case of skin infected or wounded by bacteria, there is a problem that the healing is not easy and the infection and the wound are more spread. However, since the cosmetic soap according to the present invention contains an extract of a mushroom extract and a cereal mixture, it can be prepared as a cosmetic soap having excellent skin improvement effect, in particular, a skin whitening effect and an antioxidant effect, and excellent safety. By using the cosmetic soap, the above problems can be solved. That is, when using the cosmetic soap according to the present invention, the biologically active ingredients contained in the soap during washing or bathing is dissolved with the soap, grain extract and nutritional substances contained in the situation to penetrate the skin to enhance skin beauty, Various germs of the skin are removed by the antibacterial and antimicrobial components, thereby preventing the onset of various skin diseases such as acne and the like.

On the other hand, the cosmetic composition according to the present invention is a skin whitening cosmetics, skin anti-aging cosmetics, wrinkle improvement cosmetics, skin elasticity improvement cosmetics, skin moisturizing cosmetics, skin damage prevention cosmetics, anti-viral acne treatment cosmetics, Jawasun It can be very usefully used to manufacture cosmetics such as barrier cosmetics.

Hereinafter, although an Example is given and this invention is demonstrated in detail, this invention is not limited only to these Examples.

Example 1 Ethanol Extraction of Grain Mixture

Sorghum, black beans, white beans, red beans, and black sesame seeds were mixed in a weight ratio of 1: 2: 1: 2: 2, washed with water, dried naturally, and finely pulverized with the crusher. Filtration with a 50-150 mesh filter network. 2 kg of 75% ethanol was added to 1 kg of the grinding mixture and heated with stirring at 75 ° C. for 4 hours. This heated extraction solution was removed by centrifugation at 3,000 rpm for 10 minutes to precipitate the residue. Ethanol was removed in a manner that the residue was removed under reduced pressure using a rotary evaporator (Iyla, Japan) at a temperature of 700 mmHg at 60 ° C., and the ethanol-free extract solution was removed. Lyophilization gave a grain extract (hereinafter referred to as 'G-EtOH').

Example 2 Hot Water Extraction of Grain Mixture

Sorghum, black beans, white beans, red beans, and black sesame seeds were mixed in a weight ratio of 1: 2: 1: 2: 2, the mixture was washed with water, and then dried naturally, and the dried product was finely ground with a grinder. It filtered with the 50-150 mesh filter net. 1 L of water was added to 1 kg of the ground mixture, followed by heating with stirring at 100 ° C. for 2 hours to extract the water-soluble physiologically active component contained in the grains. This heated extraction solution was removed by centrifugation at 3,000 rpm for 10 minutes to precipitate the residue. Ethanol was removed by concentrating the hot water extract solution from which the residue was removed under reduced pressure at 60 ° C. under 700 mmHg. The extract was freeze-dried to obtain a grain extract (hereinafter, 'G-W'). ').

Example 3 Extraction of Hot Water from Situation Mushroom Mycelium

Grain germination mushroom ( Phellinus linteus ) mycelium was washed with water, and then extracted by boiling water at 105 ° C for 2 hours by adding distilled water twice the weight, and after extraction, the aqueous layer was transferred to another container Extracted once more by the method and combined with the primary extraction solution. The extract was filtered through 260 mesh and concentrated under reduced pressure to 700 Brim Hg at 60 ° C. to 25 Brix (hereinafter referred to as “P-W”).

Example 4 Component Analysis of Each Extract

Table 1 shows the results of analyzing the general and mineral components of the grain extract and the situation mushroom mycelium extract prepared above in accordance with the provisions of No. 2000-18 of the Korea Food and Drug Administration. As a result of comparing each component, crude protein and crude fat content of G-EtOH was higher than G-W, but crude fiber and carbohydrate contents were extracted from G-W. In the case of P-W, the ratio of crude protein, crude fiber, and carbohydrates was relatively high. In addition, the content of minerals, including calcium, was much higher than that of grain extracts, and heavy metals such as mercury and selenium were not detected in all three extracts.

General Ingredient Analysis G-EtOH G-W P-W moisture(%) 7.3 6.8 91.5 Ash content (%) 3.8 3.7 2.1 Crude Protein (%) 8.1 6.5 3.9 Crude fat (%) 0.5 0.2 0.2 Crude fiber (%) 0.3 0.6 0.4 carbohydrate(%) 79.2 82.2 1.9 Aluminium (mg / kg) 10.7 17.8 4.2 Calcium (mg / kg) 411.4 347.9 821.1 Potassium (mg / kg) 330.6 221.3 484.3 Magnesium (mg / kg) 117.7 233.7 43.5 Manganese (mg / kg) 64.8 42.3 02 Sodium (mg / kg) 132.9 117.1 2259.1 Phosphorus (mg / kg) 89.7 113.7 1517.0 Mercury (mg / kg) - - - Selenium (mg / kg) - - -

Example 5 Ultraviolet Absorption Spectrum

One of the basic requirements for the whitening effect is the ability to absorb ultraviolet rays. Therefore, as a result of analyzing the absorption capacity of each sample and the composite to ultraviolet rays (Fig. 1), the mixture of G-EtOH and PW (4: 1, dry weight%, A) was in a wide range from 300 to 240 nm. It was found to have strong ultraviolet absorbing ability, and it was confirmed that the mixture of GW and PW (4: 1, dry weight%, C) had strong absorbing power between 280 and 245 nm. Albumin (B) or grain extract (G-EtOH: G-W = 1: 1, dry weight%, D) was found to be less absorbent than the situation mushroom mycelium extract or at a longer wavelength. From these results, it can be seen that the mixture of G-EtOH and P-W or the mixture of G-W and P-W has a higher ultraviolet absorbing effect than when used alone.

Example 6 Free Radical Scavenging Ability

Each sample mixture was added to 0.5 ml of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl methanol solution, and then 1 ml of 10% ethanol solution was added, followed by standing at 37 ° C. for 10 minutes, followed by 517 nm. Absorbance was measured at. The free radical scavenging ability was expressed as a free radical concentration of 50% (SC50), the content was measured by the following equation.

Free radical inhibition rate (%) = [1- (sample group-control group) / reference group] × 100

Comparing G-EtOH and G-W of cereals, ethanol extract showed stronger free radical scavenging ability. In the case of P-W, there was a weaker free radical scavenging ability than the grain extract, but there was no significant difference from G-W. G-EtOH and PW (4: 1 mixture, dry weight%) and GW and PW (4: 1 mixture, dry weight%) mixtures were found to be stronger than those using the samples, among others. The mixture of G-EtOH and PW showed the most added activity. From these results, it can be seen that the mixture of G-EtOH and P-W or the mixture of G-W and P-W shows synergy in free radical scavenging ability.

Free radical scavenging ability           Sample concentration (%) 0.2 0.4 0.6 0.8 1.0 2.0 G-EtOH 18 30 48 62 76 83 G-W 22 34 46 58 67 78 P-W 27 34 45 50 62 78 G-EtOH and P-W (4: 1 mixed, dry weight%) 32 42 54 71 83 88 G-W and P-W (4: 1 mixed, dry weight%) 28 36 51 73 77 85 Vitamin c 28 32 34 48 65 72

Example 7 Effect on Tyrosinase Activity

Tyrosinase is an enzyme that helps to stimulate the oxidation of tyrosine in vivo to produce melanin. In other words, if the activity of this enzyme is strong, it means that melanin is actively produced, and if it can inhibit the activity of the enzyme, it can be said to have a relatively whitening effect (Pomerantz, SH, J. Biochem., 24, 1966). ). The samples were prepared at a concentration of 1 mg / ml, and 15 µl each was put into a microplate (96 wells), 150 µl of 0.1 M phosphate buffer (pH 6.86) and 25 µl of 1.5 mM L-tyrosine solution, followed by 2.5 units / ml tyrosinase. 7 μl (mushroom tyrosinase, Sigma, USA, 0.05 M phosphate buffer, pH 6.8) was added, and the reaction was carried out at 37 ° C. for 10 minutes, and then the absorbance was measured at 490 nm.

% Inhibition = 《((D-C)-(B-A)] / (D-C) '' × 100

A: absorbance before reaction of the inhibitor

B: Absorbance after the reaction with the inhibitor

C: Absorbance before reaction of without inhibitor

D: absorbance after the reaction without the inhibitor

The inhibitory activity against tyrosinase was calculated as a concentration (IC ₅₀ ) that inhibits the activity against 50% when the absorbance of the untreated group is 100. The results are shown in Table 3 below. In the case of a mixture of G-EtOH and PW (4: 1 mixture, dry weight%) and a mixture of GW and PW (4: 1 mixture, dry weight%), the ability to inhibit tyrosinase activity was stronger than that of using the sample, respectively. Among them, G-EtOH and PW (4: 1 mixture) mixture showed the strongest inhibitory ability. From these results, it can be seen that the mixture of G-EtOH and PW or the mixture of GW and PW exhibits a synergistic effect on tyrosinase inhibitory activity.

Inhibitory effect of tyrosinase activity (%) sample Mushroom Tyrosinase (IC ₅₀ ) G-EtOH 0.415 G-W 0.447 P-W 0.387 G-EtOH and P-W (4: 1 mixed, dry weight%) 0.299 G-W and P-W (4: 1 mixed, dry weight%) 0.318 Kojic acid (Sigma, USA) 0.042 Hydroquinone (Sigma, USA) 0.016 Arbutin 0.422

Example 8 Antioxidant Effect

Linoleic acid was used to verify the effect of each extract to inhibit the automatic oxidation. This is for verifying the antioxidant effect. After adding 3 ml of 10 mM linoleic acid solution and 0.075 ml of sample extract (4 mg / ml) to an 8 ml cap tube, the mixture was mixed well and left at 4 ° C. for 24 hours. 4.7 ml of 75% ethanol aqueous solution was added to 0.1 ml of the reaction solution, followed by mixing, 0.1 ml of 30% ammonium thiocyanate solution and 0.1 ml of ferrous chloride reagent were added and reacted for 3 minutes, followed by absorbance at 500 nm. Was measured. Antioxidant effect was measured in comparison with the control group without addition of the sample. As a result, the G-EtOH and P-W mixtures showed an antioxidant activity of 87.5%, and the G-W and P-W mixtures were 83.4%. It was about the same level as vitamin C, and it was confirmed that there was a significantly stronger antioxidant power than using each extract.

Antioxidant Effect on Linoleic Acid sample Antioxidative effect(%) G-EtOH 52.9 G-W 50.7 P-W 54.8 G-EtOH and P-W (4: 1 mixed, dry weight%) 87.5 G-W and P-W (4: 1 mixed, dry weight%) 83.4 Vitamin c 85.4 Arbutin 93.2

Example 9 Preparation of Cosmetics

The skin condition of 24 Samyuk University students aged 21 to 24 years using the nutritional cream prepared in the ratio of Table 5 was examined, followed by moisturizing, T-zone oil (dryness), U-zone oil (oil level), and pores. Four items, size and elasticity, were measured. Wrinkles were excluded from the measurement because they were few because they were young students. As a test method, a nutrition cream was used on the left side of the subject and a control cream was used on the right side for 3 months. The skin condition was measured on both sides of the face prior to the use of the cream, and the same site was remeasured three months after the use of the cream. Skin measurement was performed in a constant temperature and humidity room at 24 ° C. and a relative humidity of 40%. Wrinkles at the tail of the eyes were measured using a Visiometer system (C & K Co., Ltd.). As a result, only three skin conditions (Figs. 2, 3, 4) of the subjects of the present invention are shown as an example, and most of the moisturizing power was improved, and T-zone oil (dryness), U-zone oil (oil level), and skin elasticity It has been shown to improve (Table 6).

Composition of nourishing cream (cream lotion) (unit: weight%) Material name Nutrition Cream Control Stearic acid 2.50 2.50 Wax 1.00 1.00 Glycerin Stearate 1.20 1.20 Polyglyceryl-3 Methylglucose Distearate 0.50 0.50 cooking oil 3.00 3.00 Butyl paraffin 0.20 0.20 Squalene 1.50 1.50 Octyldodecyl Myristate 2.00 2.00 Butylene glycol 3.00 3.00 Tocopherol 1.50 1.50 Linolenic acid 1.00 1.00 Dimethicone 0.50 0.50 Methyl paraffin 0.15 0.15 Imidazolidinyl urea 0.15 0.15 EDTA 0.05 0.05 Panthenol 1.50 1.50 lecithin 1.00 1.00 glycerin 8.00 8.00 Butylene Glycol 2.00 2.00 Hyaluronic acid 3.50 3.50 Diethanolamine 0.30 0.30 Polyacrylamide 0.80 0.80 Carbomer 0.12 0.12 Brewer's yeast 0.30 0.30 G-EtOH and P-W (4: 1 mixture) 10.00 0.00 Water soluble chitosan 1.00 1.00 Plantain extract 1.30 1.30 water Adjust to 100% Adjust to 100% Sum 100 100

Skin condition change by nourishing cream Metric Nutrition Cream Control Before application After application Before application After application Moisturizing low 6 2 6 4 usually 12 9 12 11 high 6 13 6 9 T zone oil lack 7 3 7 5 normal 12 17 12 13 plethora 7 4 7 6 U zone oil lack 16 8 16 12 normal 6 13 6 10 plethora 2 3 2 2 Skin elasticity bad 3 2 3 3 usually 15 10 15 14 good 6 12 6 7 wrinkle little 21 23 21 21 usually 3 One 3 3 many 0 0 0 0

Example 10 Effect on Skin Elasticity of Nourishing Cream

To test the effect of grain extract on skin elasticity, the nutrition cream was applied twice a day to the left eye edge of 20 middle-aged women aged 30 to 35 years. The right eye margin was treated with the cream of the control. Skin elasticity of the skin surface was measured 60 days after each treatment by Cutometer SEM 474. Cutometer SEM 474 is to measure the depth of wrinkles, the elasticity is expressed as a reduced value compared to the control in%, it was expressed as a flat spread value of 20 subjects. The control was almost unchanged, but it was confirmed that the effect is excellent in the treatment applied to the nourishing cream, it was proved that the cosmetics of the present invention can effectively cure skin wrinkles due to skin aging as well as elasticity of the skin.

Change of Skin Elasticity by Nutritional Cream Treatment content(%) 0 days 30 days 60 days Nutrition Cream 11.8 20.6 38.9 Control 10.7 12.4 13.8

Example 11 Formulation

In a comprehensive review of the above examples, the composite of the grain extract and the situation mushroom was able to demonstrate that the skin has anti-aging, whitening effect, antioxidant, and antiviral. Therefore, it was decided to use the cosmetic formulation in the following formulation.

Formulation Example 1. Cosmetic lotion (skin lotion)

Raw material name content(%) G-EtOH and P-W (4: 1 mixture) 1.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Water Soluble Chitosan 0.4 ethanol 10.0 Brewer's yeast 0.2 Triethanolamine 0.1 Purified water Adjust to 100 Sum 100

Formulation Example 2 Massage Cream

Raw material name content(%) G-EtOH and P-W (4: 1 mixture) 0.5 Beeswax 9.0 Polysorbate 60 2.0 PIO 60 Hardened Castor Oil 2.3 Brewer's yeast 1.0 Liquid paraffin 38.0 Squalane 7.0 Caprylic 3.5 glycerin 5.5 Butylene glycol 2.5 Propylene glycol 3.0 Water soluble chitosan 0.3 Preservative, coloring, flavoring Quantity Purified water Adjust to 100 Sum 100

Formulation Example 3. Pack

Raw material name content(%) G-EtOH and P-W (4: 1 mixture) 0.5 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Nonylphenyl Ether 0.3 Polysorbate 60 0.3 Water soluble chitosan 0.3 Preservative, coloring, flavoring Quantity Purified water Adjust to 100 Sum 100

Formulation Example 4 Cosmetic Soap

Cereal extracts (ethanol and hot water extracts), turmeric mushrooms, turmeric mushroom mycelium extracts, and extracts obtained by the method described above are mixed to add 1 to 50% by weight to a known soap composition, and the same method as in the conventional soap manufacturing method. Soap is prepared using and used as a skin soap for preventing skin aging.

As described above, the composition according to the present invention has a very good free radical scavenging ability, tyrosinase activity inhibitory activity, antioxidant effect and UV blocking effect, thus preventing skin aging, wrinkle improvement, skin elasticity increase, skin whitening, magnetic line It can be usefully used as a cosmetic composition for the purpose of blocking, increasing moisturizing power, and the like.

1 is a mixture of an ethanol extract of a grain mixture and an extract of brown rice or juvenile germ mushroom mycelium (hereinafter referred to as a situation mushroom mycelium) (A), albumin (B), a hydrothermal extract of the grain mixture and an extract of the mushroom fungus mycelium It is a figure which shows the absorption spectrum with respect to the wavelength of each ultraviolet region with respect to the mixture (C), the extract of the grain mixture (D), etc. FIG.

2, 3 and 4 is a diagram showing the skin improvement effect after 60 days of nutrition cream treatment.

Claims (10)

To the total dry weight of the composition, 1 to 99% by weight of the cereal extract obtained by extracting a cereal mixture containing sorghum, black beans, white beans, red beans, and black sesame seeds, and 99 to 1% by weight of brown rice or yul germination mushroom extract Cosmetic composition containing. According to claim 1, wherein the extract of the cereal mixture, the mixing ratio of sorghum, black beans, white beans, red beans, and black sesame seeds by weight 1: 1, 2: 1: 2: 2, respectively, and the grain mixture After finely pulverizing with a pulverizer, 0.5 to 10 times of usable organic solvents such as ethanol, methanol, isopropyl alcohol, and the like were added to 1 kg of the dry pulverized mixture obtained by filtration through a 50 to 150 mesh filter net. A cosmetic composition, which is obtained by an organic solvent extraction method of extracting grains by heating at 50 to 95 ° C. for 30 minutes to 12 hours or more. The extract of claim 1, wherein the extract of the grain mixture is mixed with sorghum, black beans, white beans, red beans, and black sesame seeds in a ratio of 1: 2: 1: 1: 2, and finely pulverized the mixture with a grinder. To 1 kg of the dry pulverized mixture obtained by filtration through a 50-150 mesh filter network, 0.5-10 times of water was added, followed by a hot water extraction method heated at 100 ° C. for 30 minutes to 12 hours or more. Cosmetic composition characterized in that. The method of claim 1, wherein the grain germination mushroom extract is added 1 to 3 times the amount of distilled water to brown rice or germinated mushroom mushroom mushroom, brown rice or germinated mushroom mushroom mycelium, or a mixture thereof, and 1 to 3 at 105 ℃ to 115 ℃ After repeating the boiling water extraction process for 2 to 3 times, concentrated under reduced pressure to obtain a situation mushroom extract containing a polysaccharide component in the extract, and then the resulting extract is filtered through 260 mesh, 700 mm at 60 ℃ Concentrated under reduced pressure with Hg, a cosmetic composition, characterized in that obtained by the method of 25 Brix (Brix). Cosmetics for skin whitening comprising a cosmetic composition according to any one of claims 1 to 4. A skin anti-aging cosmetic comprising a cosmetic composition according to any one of claims 1 to 4. A cosmetic for improving wrinkles, comprising the cosmetic composition according to any one of claims 1 to 4. Cosmetics for skin moisturizing comprising the cosmetic composition according to any one of claims 1 to 4. A sunscreen cosmetic comprising a cosmetic composition according to any one of claims 1 to 4. Cosmetic soap comprising the cosmetic composition according to any one of claims 1 to 4.