KR101207559B1 - Cosmetic composition comprising the extract of Pourthiaea villosa as active ingredient - Google Patents

Abstract

The present invention is Yunnori ( Pourthiaea) villosa ( Thunb ) Decne . there is . The present invention relates to a cosmetic composition containing villos ) extract as an active ingredient, and provides a cosmetic composition as an active ingredient, and the extract according to the present invention has an antioxidant effect, an MMP-1 production inhibitory effect, a collagen biosynthesis effect, Melanin production inhibitory effect, inflammatory cytokine expression inhibitory effect, anti-inflammatory effect, skin irritation effect, moisturizing effect, antibacterial effect and wrinkle improvement effect can be used as an active ingredient of various functional cosmetics.

Description

Cosmetic composition comprising the extract of Yunni-riku as an active ingredient {Cosmetic composition comprising the extract of Pourthiaea villosa as active ingredient}

The present invention relates to a cosmetic composition containing the extract of Yunni as an active ingredient.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into intrinsic aging and photo-aging, and research on this has been actively conducted. UV rays, stress, disease conditions, environmental factors, wounds, and free radicals may be caused by aging, which intensifies the body's ability to destroy antioxidant defenses and damage cells and tissues. And aging. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency.

Therefore, it is necessary to protect the cell membrane by eliminating free radicals generated by metabolic processes of the body, ultraviolet rays, and inflammatory reactions.Also, damaged cells must be regenerated by active metabolism to proliferate the cells. The skin can recover quickly and maintain healthy skin.

Aging involves not only these free radicals, but also enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation.

Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Until now, the raw materials used as cosmetic materials mostly inhibited only the enzyme activity. Therefore, we tried to regulate the expression and activity of MMP expression induced by intracellular aging and photoaging.

On the other hand, skin changes due to pigment deposition occur. Factors that determine skin color include basic or partial pigmentation, such as tanning due to blemishes, freckles, and sun exposure, other than acne, scars, and the distribution of acne and skin, except for differences in race, region, sex, and age. Blood circulation, stress, and health. Among the above factors, the most important factor is pigmentation.

Pigments that affect skin color include melanin, melanoids, carotene, and hemoglobin, the most important of which is melanin, the most important factor affecting the biosynthesis of melanin is the amount of ultraviolet rays and hormones in the body.

Melanin plays a major role in preventing or damaging the skin from ultraviolet rays by absorbing or scattering ultraviolet rays. There is no special maximum absorption wavelength and it absorbs light in all areas. In addition, it has excellent ability to remove reactive oxygen species, sometimes melanin itself generates active oxygen, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows free radical properties Pray.

The biosynthesis of melanin occurs through a series of oxidation processes, beginning with the conversion of tyrosine, an amino acid, from the melanocytes of melanin-forming cells into tyrocinase and into dihydroxy phenylalanine. It is formed from a polymer of eumelanin.

This biosynthesis process is carried out in a special type of brown cells in organelles called melanosomes. Melanosomes, including melanin granules, move from the periphery of the nucleus to the dendritic end of the dendritic projections and into the cytoplasm by the action of keratinocytes. Accumulate around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to the surrounding keratinocytes are partially affected by hormones and ultraviolet rays, and primarily by genetic influences. In addition, it is known that metal ions such as cytokines, copper, zinc, and iron, interferon, prostaglandin, histamine, etc., which are involved in the expression of tyrosinase and synthesis and transfer of melanin, are involved.

Ascorbic acid (JP-A 4-9320), hydroquinone (JP-A-192062), kojic acid (JP-A-56-7710), arbutin (JP-A 4-93150), and some extracts of tyrosina Although it is used as a whitening cosmetic because of its anti-ageing activity, its use is limited due to its low stability in cosmetic formulations, which is degraded and colored, causing off-flavor, efficacy at the biological level, unclear effect, and safety problems. to be. The above substances have been demonstrated to be effective in inhibiting tyrosinase, but the effect is low in experiments similar to the actual biolevels. Therefore, the evaluation of the inhibitory effect by melanoma cell culture similar to the biological level is required.

Hydroquinone is also defined as a carcinogenic substance and its use in cosmetics is prohibited or restricted. Kojic acid and ascorbic acid are very unstable substances. Cosmetics containing a small amount of the above components may cause browning when stored for several weeks at room temperature.

In general, functional substances used in many products related to the skin have an effective effect on the skin and may also cause skin irritation. Cosmetics using a variety of ingredients contain substances that can cause irritation or inflammation, and more than 10% of people who use personal care products complain of side effects on the skin. Is on the rise. More than 30-40% of respondents say they are sensitive skin in a consumer survey, and this trend continues to increase. Moreover, recent cosmetic development trends are focused on functional cosmetics (cosmeceutical) that can have a real effect on the skin, the skin side effects due to the use of personal care products such as cosmetics is expected to increase gradually. Generally, various raw materials are used to make cosmetics, and usually 20 to 50 raw materials are used. These are ingredients that are indispensable for the preservation, maintenance, and function of cosmetics such as preservatives, surfactants, sunscreens, and whitening agents, and may cause skin irritation in some of the products actually used. These ingredients have been proved to be safe through skin irritation tests, but they have been applied to the product, causing irritation, itching, stinging and burning on the skin, and erythema and edema. May cause irritation. In particular, the environmental pollution is severe and the skin is easily damaged by the increase in the amount of ultraviolet radiation due to the destruction of the ozone layer, the drying of the living space, the constitution of the individual's allergies, the stress, the chemical harmful substances.

There are many substances that are used for anti-inflammatory purposes so far, but some raw materials may not be used in cosmetics or they may be used in a limited amount, and they may be difficult to stabilize or have poor solubility. It has a difficult problem.

In this context, antibiotics such as erythromycin, tetracycline, and sindamycine, which are antibiotics that have an inhibitory effect on acne-causing bacteria, cannot be used as cosmetics. Because of this problem, various studies have been conducted to develop an antimicrobial substance using the property that the antimicrobial substance produced by the microorganism has the ability to inhibit a wide range of microorganisms and has low selective toxicity to higher organisms.

In order to solve these various problems, cosmetics using natural products have recently been developed to reduce skin irritation caused by various chemicals. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

Therefore, the present inventors have studied the possibility of applying to cosmetics in a variety of natural products that have not been known so far, selecting a rosaceae yunnori (ginyun nori, true yunnori, jeongjinori nori) and to extract extracts from the skin, Antioxidant effect, MMP-1 production inhibitory effect, collagen biosynthesis effect, melanin production inhibitory effect, inflammatory cytokine expression inhibitory effect, anti-inflammatory effect, skin irritation effect, moisturizing effect, antibacterial effect and wrinkle improvement effect Since this is very good, it was found that the efficacy as a cosmetic can be expected.

The purpose of the present invention is to contain the extract of Yunri tree, antioxidant effect, MMP-1 production inhibitory effect, collagen biosynthesis effect, melanin production inhibitory effect, inflammatory cytokine expression inhibitory effect, anti-inflammatory effect, skin irritation effect, moisturizing effect, antibacterial effect And to provide a cosmetic composition exhibiting an anti-wrinkle effect.

It is also an object of the present invention to provide a cosmetic composition that maximizes the efficacy and effect of Yunni-tree extract using solvent extraction, supercritical extraction, ultrasonic extraction, fermentation or foaming.

Another object of the present invention is the antioxidant composition, MMP-1 production inhibitory effect, collagen biosynthesis effect, melanin production inhibitory effect, inflammatory cytokine expression inhibitory effect, anti-inflammatory effect when applying the cosmetic composition containing the Yunri tree extract to human skin To provide a cosmetic method for obtaining skin irritation, moisturizing, antibacterial and antiwrinkle effects.

Therefore, the object of the present invention as described above is achieved by the cosmetic composition for antioxidant containing Yunni wood extract as an active ingredient.

Another object of the present invention is achieved by a cosmetic composition for improving skin wrinkles containing Yunni extract as an active ingredient.

Another object of the present invention is achieved by a cosmetic composition for skin whitening containing the extract of Yunni wood as an active ingredient.

Another object of the present invention is achieved by an anti-inflammatory cosmetic composition containing the extract of Yunni as an active ingredient.

Another object of the present invention is achieved by the antimicrobial cosmetic composition containing a Yunni extract as an active ingredient.

Another object of the present invention is achieved by a moisturizing cosmetic composition containing the extract of Yunni.

Still another object of the present invention is achieved by a cosmetic composition for alleviating skin irritation, which comprises the extract of Yunri as an active ingredient.

Preferably, the Yunri tree extract is characterized in that it contains 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, preferably, the Yunni tree extract is (a) water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, Solvent extraction using an extraction solvent selected from the group consisting of dichloromethane, hexane and mixtures thereof, (b) ultrasonic extraction, (c) supercritical extraction, (d) fermentation or (e) foaming. Also, preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray It is characterized by having a formulation selected from the group consisting of.

Another object of the present invention is to apply the cosmetic composition to the skin of the human, characterized in that to obtain an antioxidant effect, skin wrinkle improvement effect, skin whitening effect, anti-inflammatory effect, antibacterial effect, skin moisturizing improvement effect or irritation relief effect It is achieved by the makeup method.

Yunni tree extract according to the present invention skin antioxidant effect (Experimental Example 1), MMP-1 production inhibitory effect (Experimental Example 2), collagen synthesis enhancement effect (Experimental Example 3), melanin production inhibitory effect (Experimental Example 4), inflammatory Cytokine expression suppression effect (Experimental Example 5), anti-inflammatory effect (Experimental Example 6), stimulation alleviation effect (Experimental Example 7), moisturizing effect (Experimental Example 8), antibacterial effect (Experimental Example 10) and wrinkle improvement effect (Experimental example) 10)

In addition, according to the present invention, in order to maximize the efficacy of the yunni extract, the antioxidant effect, MMP-1 production inhibitory effect, collagen synthesis inhibitory effect, melanin production inhibitory effect through solvent extraction method, ultrasonic extraction method, supercritical extraction method, fermentation method or preparation method It further enhances the effects and inflammatory cytokine expression suppression, anti-inflammatory, skin irritation, moisturizing, antibacterial effect.

Yunno tree (Pourthiaea villosa) used as an active ingredient in the cosmetic composition of the present invention is a tree belonging to the Rosaceae family and is commonly found in south Korea and is commonly seen in Jeju Island. Stems grow from bottom to side, with several trunks rising. The young branches have white hairs and elliptical branches. The leaves are 5 ~ 10 cm long, rounded, and regenerated, with sharp and sharp teeth on the edges. The flower is white in May and is 3 ~ 5 cm in diameter and has 5 petals and sepals each. Roots are known to be effective in dysentery and diarrhea, and there are no specific ingredients known.

In the present invention, the yunniri extract means that obtained by extracting from various organs or parts (e.g., leaves, flowers, roots, stems, branches, bark and seeds, etc.) of the yunniri, preferably leaves, stems, branches , An extract obtained from the bark or the root, more preferably an extract obtained from the leaf, the branch, the bark or the root, and most preferably the extract obtained from the branch or the bark.

In addition, the Yunri tree extract of the present invention may be prepared according to a conventional method known in the art, that is, using a conventional solvent, under the conditions of conventional temperature and pressure, preferably (a) an extraction solvent Extraction using solvent (b) Supercritical extraction by reduced pressure with carbon dioxide and high temperature, (c) ultrasonic extraction, (d) fermentation or (e) foaming.

In the present invention, the extract of Yunri tree using the solvent extraction method using the extraction solvent is a variety of extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (for example, methanol, ethanol, propanol and butanol), propylene Obtainable using an extraction solvent selected from the group consisting of glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, and mixtures thereof. Preferably it is obtained using ethanol, 70% (v / v) ethanol aqueous solution or water, most preferably 70% (v / v) ethanol.

On the other hand, it will be apparent to those skilled in the art that the Yunri tree extract of the present invention can obtain an extract having substantially the same effect using not only the aforementioned extraction solvent but also other extraction solvents.

According to a preferred embodiment, the method of producing a Yunni tree extract of the present invention is as follows. First, the branches or bark of Yunri tree are washed with purified water, dried and crushed into small pieces so that the particles have a size of 300 mesh or less, followed by 1 to 10 times the dry weight of water, 1 to 4 carbon atoms, or Hydrous lower alcohol, acetone, ethyl acetate, butyl acetate or 1,3 butylene glycol; After cooling condenser is installed to prevent the active ingredient from evaporating in the state of extraction at 40 ~ 100 ℃ heated for 3 to 20 hours, or extracted at 4 ~ 40 ℃ for 1 to 15 days and completely dried by a rotary vacuum evaporator to prepare . In this case, 1,3 butylene glycol is difficult to dry by using a rotary vacuum evaporator, so it is directly extracted in the above conditions and then dried in a hot air dryer at 30 ℃ ~ 60 ℃ so that the drying loss 1% (w / w) We use for cosmetics.

In addition, the yunniri extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract through a conventional purification process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using ultrafiltration membranes with constant molecular weight cut-off values, various chromatography (size, charge, hydrophobicity or affinity) Active fraction obtained through various purification methods additionally carried out, such as separation by sex)) is also included in the yunniri extract of the present invention.

Further, the present invention provides a cosmetic composition characterized in that the above extract is obtained by liquid-freezing obtained by cold-pressing and heating filtration at room temperature, and further by concentrating or lyophilizing the solvent under reduced pressure.

The extraction by supercritical extraction by decompression by carbon dioxide and by high temperature means supercritical fluid extraction. Generally, supercritical fluid is a liquid having a gas when the critical point is reached at high temperature and high pressure. It is gaseous, chemically similar in polarity to nonpolar solvents, and because of its properties, supercritical fluids are used for extraction of fat-soluble substances (J. Chromatogr. A. 1998; 479: 200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gaseous properties as the pressure and temperature reach a critical point through the operation of a supercritical fluid device, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substance contained in the sample is extracted from the supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a co-solvent is added to carbon dioxide.

Such cosolvents are selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether and mixtures thereof.

Most of the extracted samples contain carbon dioxide. Since carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method may be used as a cosmetic composition, and the cosolvent may be removed by a reduced pressure evaporator.

As an example of a method for producing a Yunni-tree extract by supercritical extraction, the branches or bark of Yunni-tree are washed with purified water, dried and broken into small pieces of 300 mesh or less, and then the sample is placed in a supercritical extraction vessel and subjected to liquid chromatography. Using a graffiti pump, pure carbon dioxide is supercritical at the proper temperature and pressure and flows to the test tube while extracting it.

In supercritical extraction, carbon dioxide is at a temperature of 35 to 100 ° C., preferably 50 to 70 ° C., more preferably about 60 ° C., 100 to 500 atmospheres, preferably 200 to 400 atmospheres, more preferably about 300 Supercritical is recommended under pressure.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration. Ultrasonic waves can destroy an insoluble solvent contained in a sample in a water-soluble solvent. Due to the high local temperature generated at this time, Since the kinetic energy of the particles is increased, sufficient energy required for the reaction is obtained. By inducing the high pressure by the impact effect of the ultrasonic energy, the mixing efficiency of the substance and solvent contained in the sample is increased and the extraction efficiency is increased.

The extraction solvent that can be used in the ultrasonic extraction method is selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether and mixtures thereof.

The extracted sample is vacuum filtered to recover the filtrate and then removed by a reduced pressure evaporator, the extract can be obtained through a conventional method of producing a yunniri extract extract lyophilized.

Ultrasonic extraction method of Yunni tree extract of the present invention is an extraction solvent selected from the branch of the Yunni tree, the bark or branched Yunni tree, the bark of chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether and mixtures thereof After putting in, using an ultrasonic extractor (Supernonic, United States) to extract for about 1 to 6 hours at 30 ℃ at an intensity of 25KHz, the ultrasonic extraction time may vary depending on the amount of sample to be extracted.

In addition, the yunniri extract of the present invention also includes an extract that has undergone a conventional fermentation process using a microorganism. At this time, the microorganisms available can be selected from the group consisting of yeast, lactic acid bacteria, Bacillus bacteria and mixtures thereof, and fermented for 1 to 7 days while maintaining pH 5 to 7 under temperature conditions of 20 to 40 ℃.

The yeast used to ferment the limbus of the present invention belongs to eukaryotic microorganisms (organisms in which the nucleus and cytoplasm are divided by the nuclear membrane) and have cell organelles such as cell walls, cell membranes, nuclei, private bodies, and granules. Those without sex distinction breed mainly by budding, among which many of the isolated yeasts have no fermentation capacity, multiply by fission or bud-fission instead of budding, and not ascospore. There are also various groups of yeasts, such as forming spores or not forming spores. The size is usually 5-10 ㎛, 5-10 times the size of bacteria, and the optimal pH survives at 4.5-5.5 but 1-10. Yeasts that survive 12 hours at pH 1 are more resistant to acids than bacteria, so adjusting the conditions of the medium to pH 3.5 to pH 3.8 can suppress bacterial contamination to some extent.

Yeast is slightly different depending on the species, but most grow well at 20 ~ 25 ℃. Usually, it is killed above 50 ℃, but it can be overcome to some extent by adding the growth factor which is not synthesized above the temperature directly to the medium. Saccharomyces cerevisiae dies at over 40 ° C but can be prevented by adding oleic acid and ergosterol to the medium. Yeast cells themselves are single-celled plants that contain large amounts of protein and trace minerals. Thus, a preferred yeast for use in fermenting the limbus of the present invention is Saccharomyces cerevisiae.

In addition, the lactic acid bacteria used to ferment the Yunni tree in the present invention is a kind of microorganisms which are very beneficial to the human body to break down carbohydrates such as glucose or lactose to produce organic acids such as lactic acid (lactic acid) or acetic acid. Generally, lactic acid bacteria make lactic acid from sugar is called fermentation, and fermented food is made through this fermentation process. Lactic acid bacteria that can be used in the present invention preferably include Lactobacillus, Streptococcus, Bifido bacterium, Leukonostock, Pediococcus, Lactococcus and mixtures thereof, most preferably Lactobacillus . Among the Lactobacillus, the most preferred lactic acid bacteria for use in fermenting the green beans of the present invention is Lactobacillus plantarum .

In addition, Bacillus bacteria can be used as the fermentation bacteria used to ferment Yunni in the present invention, Bacillus is spore-forming bacteria, inhabit in soil or water, and most of them are related to decomposition of organic matter. Bacillus that may be used in the present invention preferably includes Bacillus subtilis, Bacillus pumilis, Bacillus rikenimomis, Bacillus megaterium, Bacillus polyfermentus, Bacillus mesentericus and mixtures thereof, Most preferably, Bacillus subtilis, which is also known as Bacillus subtilis, is an organic substance that is used to produce fermented foods such as meju and Cheonggukjang by anaerobic metabolism of various sugars such as glucose and starch. Decomposed microorganisms.

Fermentation broth using Yunri tree of the present invention can be prepared in the following steps:

a) powdering the branches of the Yunni tree;

b) diluting the powder of the branched part of the yunnori prepared in step a) in purified water and then fermenting using yeast, lactic acid bacteria, Bacillus bacteria or a mixture thereof;

c) filtering the fermentation broth obtained by the fermentation of step b) to low temperature aging and filtering again to produce a fermented liquor.

In the manufacturing step of the Yunri-tree fermentation broth, the powder of the Yunri-tree branch portion of step a) may be prepared in a size of 100 to 500 mesh or less, but is not limited thereto.

In addition, the fermentation of step b) is mixed with yunni powder and purified water at 1:10 and sterilized at 121 ℃ and then inoculated with lactic acid bacteria (Lactobacillus sp), yeast (Sacharomyces sp) and / or Bacillus sp (Bacillus sp) Achieved by culturing. Yunni wood powder used in the fermentation step is preferably used in an amount of 1 to 50g based on 1L of the total culture.

In addition, the yeast or lactic acid bacteria and Bacillus bacteria used in the fermentation step is preferably inoculated in the amount of 1 X 10 ⁴ to 1 X 10 ⁵ cells based on the total culture solution 1L, and according to the characteristics of each strain and It may be cultured in aerobic or usually anaerobic conditions. In addition, additional carbon sources and / or pH adjusters may be added to further activate the culture of the fermentor. At this time, suitable culture conditions are preferably incubated for about 1 to 15 days at 30 ~ 37 ℃, aerobic or normal anaerobic conditions of pH 5-7, more preferably 1 to 7 days.

The step c) is the step of first filtration of the fermentation broth obtained in step b) and low temperature aging of the filtrate, wherein the filtration is filtered through a filter membrane of 0.25 to 0.45 μm size, to remove the extra grains of the powdered fermented berry Can be removed The filtrate separated by the primary filtration membrane can be stabilized by aging at low temperature, and can be stored after concentration of the secondary filtration with a finer filtration membrane than the primary filtration membrane, and the concentrate is diluted and used appropriately. At this time, the filtration-aging-filtration-concentration process is a process step for obtaining a more effective effect of the Yunri-tree fermentation broth, and the low-temperature aging process is preferably performed for 5 to 10 days in the temperature range of 0-4 ° C, and remaining In order to reliably remove the bacteria, the size of the secondary filtration membrane is preferably 0.25 μm or less.

According to a more preferred embodiment, the powder of yunniri powder below 300 mesh size and then added to the culture medium 10 g / L, yeast, lactic acid bacteria, Bacillus bacteria or a mixture thereof is added at 50,000 cfu / L each yeast 30 ℃, lactic acid bacteria at 37 ℃, Bacillus bacteria at 37 ℃, in the case of mixed bacteria selected from 30 ℃ and 37 ℃ depending on the case, pH 5-7, preferably about pH 5.5 fermentation tank in aerobic or normal anaerobic conditions Incubate for about 1 to 7 days in fermentation. After incubation, the fermentation broth was first filtered using a 0.45 μm filtration membrane, and then aged at low temperature for 10 days in the filtrate, and then concentrated by secondary filtration with a 0.25 μm membrane, thereby preparing the extract of the present invention. The final solids concentration is kept to 10 g / L before use.

In addition, the Yunri tree extract of this invention also contains the extract obtained using the foaming method. Foaming agent is a pharmaceutical technology that changes the original properties of the medicine by processing the medicine based on the herbal theory. Mainly refers to the process of steaming, boiling, roasting, roasting or roasting or a process in which these are mixed. The treatment conditions are 60 to 100 ° C. for 30 minutes to 6 hours when boiled, 120 to 180 ° C. for 10 minutes to 2 hours for steaming, and 80 to 100 ° C. for 10 minutes to 2 hours for baking. Most preferably, the steaming process is performed, followed by drying at 90 ° C. for 1 hour.

In the present invention, the extract is prepared by steaming and drying the Yunni wood, and most preferably, the extract obtained by the saponification method is further extracted using 70% ethanol. In addition, the extract using the foaming method, as well as the solvent extraction, extraction by the supercritical extraction method or the ultrasonic extraction method by a reduced pressure and high temperature with carbon dioxide can be extracted or fermented using microorganisms.

Yunni tree extract of the present invention may be prepared in a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

According to a more preferred embodiment of the present invention, the Yunni tree extract of the present invention eliminates free radicals, inhibits the production of MMP-1 and enhances the biosynthesis of collagen, inhibits melanin production of melanocytes, and is expressed by UV irradiation. To suppress the expression of inflammatory cytokines, anti-inflammatory, antibacterial and relieve skin irritation, improve wrinkles, characterized in that it comprises an effective amount of Yunri tree extract to impart moisture to the skin.

According to the present invention, the Yunri tree extract is contained 0.001 to 30.0% by weight based on the total weight of the cosmetic composition, more preferably, the Yunni tree extract is contained 0.01 to 10% by weight relative to the total weight of the cosmetic composition It features. When the content of the extract is less than 0.001% by weight, no skin improvement effect is observed, and when the content of the extract is more than 30.0% by weight, the degree of improvement of the skin improvement effect against the increase of the content is insignificant, and there is a problem in the safety and stability of the formulation and economics. Neither ever

Therefore, the cosmetic composition of the present invention comprising a extract of Yunri tree having such various functions has a skin antioxidant effect (Experimental Example 1), MMP-1 production inhibitory effect (Experimental Example 2), collagen synthesis enhancement effect (Experimental Example 3), Melanin production inhibitory effect (Experimental Example 4), inflammatory cytokine expression inhibitory effect (Experimental Example 5), anti-inflammatory effect (Experimental Example 6), stimulating relaxation effect (Experimental Example 7), moisturizing effect (Experimental Example 8), antibacterial effect ( Experimental Example 10) and wrinkle improvement effect (Experimental Example 10) is excellent.

Therefore, the cosmetic composition of the present invention comprising a Yunri tree extract having such various functions is characterized by having an excellent antioxidant effect, skin wrinkle improvement effect, skin whitening effect, anti-inflammatory effect, antibacterial effect, skin moisturizing improvement effect or irritation relief effect It is done.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention by applying a cosmetic composition containing the extract of Yunri tree as an active ingredient to the skin of humans, the antioxidant effect, skin wrinkle improvement effect, skin whitening effect, anti-inflammatory effect, antibacterial effect, skin moisturizing improvement effect or irritation relief effect It provides a make-up method characterized in that the obtained.

The cosmetic method of the present invention refers to all cosmetic methods using the cosmetic composition of the present invention. That is, all the methods known in the art using the cosmetic composition belong to the makeup method of the present invention.

The cosmetic composition of the present invention may be used once or several times, or may be used in combination with other cosmetic compositions other than the present invention. The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

If the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When the cosmetic method using the cosmetic composition comprising the extract of the present invention, the antioxidant effect of skin, MMP-1 production inhibitory effect, collagen synthesis enhancement effect, melanin production inhibitory effect, inflammatory cytokine expression inhibitory effect, anti-inflammatory effect, Antibacterial, moisturizing, irritant and skin wrinkle improvement can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  One: Velvet  Solvent extract preparation

200 g of the dried walnut branch and the bark area, which were washed with purified water and powdered, were powdered and made fine by using 300 mesh sieves. 1000 ml of an aqueous 70% (V / V) ethanol solution having a ratio of ethanol and water adjusted to 7: 3 was added thereto, and the mixture was extracted under reflux three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and freeze dried at 50 ° C. or lower, and then subjected to the experiment of the present invention.

Manufacturing example  2 to 3. Velvet Supercritical  Manufacture of fluid extracts and ultrasonic extracts

Preparation Examples 2 to 3 were obtained in the same manner as in Preparation Example 1, except for the difference in the following extraction method. Preparation Example 2 was extracted by a conventional supercritical extraction method (extracted using olive oil as a cosolvent in a supercritical state under a pressure of about 300 atm at a temperature of about 60 ℃) to obtain a supercritical extract. Preparation Example 3 was obtained through the extraction using the ultrasonic wave (extracted for about 2 hours at 30 ℃ with a strength of 25KHz using a superonic ultrasonic apparatus). However, in Preparation Example 2, ethanol was used as the cosolvent, and in Preparation Example 3, purified water was used as the cosolvent.

Manufacturing example  4. Velvet  Yeast Fermentation Extract Preparation

After washing with purified water and drying the dried walnut branch and bark area was made fine using a 300 mesh sheave. This was added to yeast culture (Potato Dextrose Broth, Accumedia, United States) to 10 g / L. Saccharomyces cerevisiae was used as a yeast strain for fermentation, and was added at 50,000 cfu / L per culture. The culture was carried out using a 5L fermenter for 7 days, maintained at 30 ℃, pH 5-7. After the culture was completed, the fermented fermentation broth was centrifuged to remove primary cultures, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged again for about 10 days in a low temperature warehouse at 4 ° C., and the filtrate was filtered again using a filter of 0.25 μM, and used for the present invention. It is called. The yunni yeast fermentation extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Manufacturing example  5. Velvet  Manufacture of Lactic Acid Bacteria Fermented Extract

After washing with purified water and drying the dried walnut branch and bark area was made fine using a 300 mesh sheave. Add 10 g / L of lactic acid bacteria culture medium (Lactobacillus MRS Broth, Difco, USA) and mix After sterilization at 121 ℃ was inoculated with lactic acid bacteria.

Lactobacillus used for fermentation was used Lactobacillus plantarum (Lactobacillus plantarum), was added at 50,000 cfu / L per culture. Cultivation was carried out using a 5L fermenter for 3 days, maintained at 37 ℃, pH 5-7. After the culture was completed, the fermented fermentation solution of Yunni-tree was centrifuged to remove primary cultures, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged again for about 10 days in a low temperature warehouse at 4 ° C., and the filtrate was filtered again using a filter of 0.25 μM. The fermentation filtrate was used for convenience in the present invention. This is called. The yunni lactobacillus fermented extract used in the experiment below was used to maintain a final concentration of 1g / 100mL.

Manufacturing example  6. Velvet Bacillus bacteria  Preparation of Fermented Extract

After washing with purified water and drying the dried walnut branch and bark area was made fine using a 300 mesh sheave. This was added to the Bacillus culture medium at 10 g / L.

Bacillus strains used for fermentation were used Bacillus subtilis ( Bacillus subtilis ), was added at 50,000 cfu / L per culture. Cultivation was carried out using a 5L fermenter for 3 days, maintained at 37 ℃, pH 5-7. After the culture was completed, the fermented broccoli fermented broth was centrifuged to remove the cultures first, followed by final filtration using 0.25 μM filter paper. The filtrate thus obtained was aged for about 10 days in a low temperature warehouse at 4 ° C., and then the filtrate was filtered again using a filter of 0.25 μM and used in the present invention. Called an extract. Moreover, the extraction yield by this method was 221.6 g / 1 kg. Fermented Bacillus Bacillus fermentation extract used in the following experiment was used to maintain a final concentration of 1g / 100mL.

Manufacturing example  7. Velvet A preparation method  Preparation of extract

In the embodiment of the present invention to obtain the extract of the yunniri tree was processed by the process of steaming and drying. In other words, after washing with dried water and dried dried Yunni trees outpost powdered by using a 300 mesh sheave, the steaming temperature was carried out at 90 ℃, 1 hour conditions and then dried. The extract of the yunni tree made in this way was extracted using additional 70% ethanol, and as a result, an extract of 53.8g / 1kg was obtained.

Experimental Example  One: DPPH Antioxidant effect measurement

In Experimental Example 1, the antioxidant effect (free radical scavenging rate) was measured by using the DPPH method to measure the antioxidant effect of the Yunni-tree extracts obtained in Preparation Examples 1-7.

The DPPH method measures the antioxidant effect by reducing power using a free group called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The free radical scavenging rate (%) is measured at a wavelength of 560 nm by comparing the degree of decrease in absorbance of DPPH by the test substance (Yunori tree extract) and the absorbance of the blank test solution.

The reagent used was 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) as 61.88 mg in methanol to make 100 ml.

As a measuring method

① Add 0.15 ml of 0.1 mM DPPH solution to 0.15 ml of 96-well plate, stir rapidly, and incubate for 10 minutes at 25 ° C.

(2) Then measure the absorbance St at 560nm.

③ The blank of the sample solution is measured in the same manner as when the absorbance St is measured except that methanol is used instead of the 0.1 mM DPPH solution to measure the absorbance So.

④ The blank test is performed in the same manner as in the case of measuring the absorbance St, except that distilled water is used instead of the sample solution to measure the absorbance Bt.

⑤ Blank in the blank test was measured in the same manner as when the absorbance St was measured except that methanol was used instead of the 0.1 mM DPPH solution and distilled water was used instead of the sample solution.

The result of free radical scavenging rate (%) was calculated by Equation 1, and the results are shown in Table 1 below.

St: absorbance at 560 nm after free radical scavenging of the sample

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: absorbance at 560 nm before reaction when free radicals are not added

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

sample Treatment concentration (%) Free radical scavenging rate ((%) antioxidant effect) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Production Example 6 Production Example 7 Cypress Tree Extract 0.0001 11.1 27.1 13.2 15.2 12.1 14.5 11.2 0.0005 32.3 36.4 29.5 35.4 30.3 40.7 29.4 0.001 59.5 64.2 54.9 66.4 60.3 60.4 50.2 0.005 87.1 85.2 84.2 89.9 86.5 88.8 82.4 0.010 93.5 90.3 91.4 92.3 91.9 90.6 90.0 Vit-c 0.05  89.9

As shown in Table 1, in the free radical scavenging experiment by DPPH, it was confirmed that the yunniri extract according to the present invention increased the antioxidant effect in a concentration-dependent manner, the yunniri extract extracts the free radical scavenging rate at 0.01% concentration ( It was confirmed that it has an antioxidant effect more excellent than that of vitamin C, a substance known to have an antioxidant effect.

Experimental Example  2. Yun-ri tree  Extract MMP -1 suppression effect

The effect of inhibiting the production of MMP-1, which is a collagen degrading enzyme, from the extract of Yunri tree obtained in Preparation Examples 1 to 7 was measured.

Fibroblasts (Korean Cell Line Bank, South Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc, Denmark) at 1 × 10 ⁶ cells per well, and treated with DMEM medium (Sigma, United States) and 37 ° C. After incubation for 24 hours in serum-free DMEM medium to which the Yunri tree extract of Preparation Examples 1 to 7 and the control group was further incubated for 48 hours in a serum-free DMEM medium not containing the Yunri tree extract.

After incubation, the supernatants of each well were collected and the amount of ng / ml newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, United States of America), and MMP-1 production according to Equation 2 below. The percent inhibition was calculated and the results are shown in Table 2.

Name of sample Treatment concentration Inhibition rate of MMP-1 production (%) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Production Example 6 Production Example 7 Cypress Tree Extract
10ppm 13.14 15.23 23.45 12.1 17.56 27.1 15.2 50 ppm 29.12 33.12 47.25 30.3 30.32 36.4 35.4 100 ppm 45.23 48.88 62.98 60.3 46.21 64.2 66.4 500 ppm 85.26 89.54 87.32 86.5 86.9 85.2 89.9 1000 ppm 88.25 92.31 93.69 91.9 92.66 90.3 92.3 TGF-β 200 nM 75.3

As shown in the results of Table 2, the Yunri tree extract of the present invention was confirmed to inhibit the production of MMP-1 (inhibition of MMP-1 activity) in a concentration-dependent manner.

In addition, even when considering the difference between the concentration of TGF-β (200nM), the concentration of the Yunri tree extract of the present invention (500ppm), which is known to have an inhibitory effect on MMP-1 production, The degree of inhibition of MMP-1 showed the same as that of the extract of the present invention, Yunni-tree extract shows that MMP-1 production is inhibited.

Experimental Example  3. Yun-ri tree  Enhancement of Collagen Synthesis by Extracts

Fibroblasts, human normal epithelial cells, were seeded in 48-well microplates at 1 × 10 ⁶ cells per well and incubated in DMEM medium for 24 hours. Serum-free DMEM medium to which the Yunri tree extract prepared by Preparation Examples 1 to 7 was added and the serum-free DMEM medium containing no Yunni tree extract as a control was further incubated for 48 hours. After the incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / Respectively. Collagen biosynthesis increase rate (%) was calculated according to the following equation (3), the results are shown in Table 3.

Name of sample Treatment concentration Increase in collagen biosynthesis (%) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Production Example 6 Production Example 7 Cypress Tree Extract
1 ppm 12.10 31.24 13.10 11.410 13.50 18.10 13.10 5 ppm 33.4 51.12 33.89 32.89 33.00 36.89 33.82 10ppm 51.50 85.87 60.59 65.59 65.00 55.59 57.54 50 ppm 82.20 108.50 85.64 81.64 83.50 75.64 82.65 100 ppm 113.50 141.35 109.98 101.98 100.95 90.98 93.98 TGF-β 200 nM  175.23

According to Table 3, it was confirmed that the collagen biosynthesis rate of the Yunri tree extract prepared by Preparation Examples 1 to 7 of the present invention increases in a concentration-dependent manner. In particular, it was confirmed that the supercritical extract of Yunni wood prepared by Preparation Example 2 had a better effect than the extracts of Preparation Examples 6 and 7.

Experimental Example  4: Yun-ri tree  Inhibitory Effect of Extracts on Melanin Production by B16F1 Melanocytes

Experimental Example 4 is to determine the whitening effect by looking at the degree of inhibition of melanin production on B16F1 melanin forming cells in order to confirm the whitening effect of the Yunri tree extract obtained in Preparation Examples 1 to 7. B16F1 melanin forming cells used in Experimental Example 4 are cell strains derived from mice, and cells that secrete a black pigment called melanin.

During the artificial culture of these cells, samples were treated to compare the degree of reduction of melanin pigment. The B16F1 melanin-forming cells used in this experiment were purchased from the American Type Culture Collection (ATCC).

The melanin biosynthesis inhibitory effect of B16F1 melanin forming cells was measured as follows. B16F1 melanin-forming cells were divided into 6-well plates at a concentration of 2 X 10 ⁵ per well, and the cells were attached to cells and incubated for 72 hours by treating the Yunni extract according to Preparation Example 1 at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, and the cells were counted and centrifuged to recover the cells. Quantification of intracellular melanin was performed with a slight modification of the method of Rotan: Cancer Res., 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin and the absorbance of melanin was measured at 405 nm with a microplate reader. The inhibition rate (%) of melanin formation was measured. The inhibition rate of melanin production of B16F1 melanin forming cells (%) was calculated by the following Equation 4, the results are shown in Table 4.

A: Melanin amount in wells without sample

B: Amount of melanin in the well to which the sample was added

Name of sample process
density
(%) Melanin production inhibition rate (%) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Production Example 5 Production Example 6 Production Example 7 Cypress Tree Extract 0.01 23.51 11.51 22.33 12.31 21.60 12.32 12.65 0.05 47.25 33.25 39.21 29.23 45.52 45.41 34.54 0.1 65.21 55.21 58.33 38.36 60.32 68.53 54.23 0.5 75.23 65.44 66.23 56.24 70.45 76.63 65.75 Hydroquinone 50 ppm 71.54 Arbutin 200 ppm  66.18

As can be seen from the results of Table 4, it was found that the purified Yunri tree extract prepared by Preparation Examples 1 to 7 of the present invention significantly inhibited melanin production in B16F1 melanin forming cells.

Experimental Example  5: Yun-ri tree  Inhibitory Effects of Extracts on Inflammatory Cytokine Expression by Ultraviolet Irradiation

Experimental Example 5 is to evaluate the inhibitory effect of the inflammatory cytokine expression expressed by ultraviolet irradiation of the Yunri tree extracts obtained in Preparation Examples 1 to 7, 24-24 fibroblasts isolated from human epidermal tissue 5 x 10 ⁴ pieces were placed in a well test plate and attached for 24 hours.

Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with 10 mJ / cm 2 of UV light using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), after which PBS was removed and FBS was not added to the cell culture medium (DMEM). Medium) 350 μl was added.

After treating the Yunri tree extract to be evaluated here, it was incubated for 5 hours. 150 µl of the culture supernatant was taken to quantify IL-1α, thereby determining the inhibitory effect on the inflammatory cytokine expression of Yunni extract. The amount of IL-1α was quantified using an enzyme immunoassay (Enzyme-linked Immunosorbent Assay), and the expression inhibition rate (%) of inflammatory cytokines (IL-1α) was calculated by Equation 5 below, and the results are shown in Table 5 below. Shown in

Bo: IL-1? Production in wells without UV irradiation

Bt: IL-1? Production in wells irradiated with ultraviolet light and not treated with the sample

St: IL-1α production amount of wells that were irradiated with UV light

Name of sample Treatment concentration Inhibitory rate of inflammatory cytokine expression (%) Preparation Example 1 Preparation Example 2 Production Example 3 Production Example 4 Production Example 5 Production Example 6 Preparation Example 7 Cypress Tree Extract 10ppm 12.10 23.99 13.99 21.21 13.01 12.32 14.12 50 ppm 23.40 35.78 25.78 32.32 24.58 32.64 21.65 100 ppm 31.50 61.33 31.33 45.54 31.00 41.34 23.56 500 ppm 42.20 67.65 47.65 65.65 45.60 54.32 54.75 1000 ppm 53.50 78.99 58.99 76.54 56.30 64.54 60.12 Indomethacin 100 ppm 61.23

According to Table 5, it can be seen that the supercritical extract of Yunni tree prepared by Preparation Example 2 inhibits the production of inflammatory cytokine IL-1α by UV at 78.99% at a concentration of 1000 ppm. It was confirmed that the Yunni-tree extract prepared by Preparation Example 2 was better in inhibiting the expression of IL-1α by about 1.47 to 1.98-fold than that of the Yunni-tree extract prepared by Preparation Example 1. In particular, it can be seen that the yunniri extract according to the present invention showed an excellent inflammatory cytokine expression rate than indomethacin, an inhibitor of inflammatory cytokine expression at the same concentration. Therefore, it can be seen that the yunniri extract of the present invention effectively protects the inflammation caused by ultraviolet irradiation even at low concentrations.

Example  One : Yun-ri tree  Extract-containing Cosmetics  Preparation of the composition

Cosmetics containing the Yunri tree extract prepared a cosmetic (Example 1 to Example 7) each containing the Yunri tree extract of Preparation Examples 1-7. At this time, for the comparison of efficacy, cosmetic composition comprising glycerin and 1,3 butylene glycol as a moisturizing agent instead of Yunni tree extract (Comparative Example 1), cosmetic composition comprising glycerin and sorbitol as a moisturizing agent (Comparative Example 2), Yunni tree Cosmetic compositions containing adenosine (Comparative Example 3) instead of extracts and cosmetic compositions (control) not containing both extracts and moisturizers are shown in Table 6 below. The effect was measured using a cosmetic containing purified water instead of extract or moisturizer as a control.

ingredient Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Comparative Example 1 Comparative Example 2 Comparative Example 3 Control group Production Example 1 5.0 - - - - - - - - - - Production Example 2 - 5.0 - - - - - - - - - Production Example 3 - - 5.0 - - - - - - - - Production Example 4 - - - 5.0 - - - - - - - Production Example 5 - - - - 5.0 - - - - - - Production Example 6 - - - - - 5.0 - - - - - Production Example 7 - - - 5.0 - - - - glycerin - - - - - - - 5.0 5.0 - - 1,3-butylene glycol - - - - - - - 5.0 - - - Sorbitol - - - - - - - - 5.0 - - Adenosine - - - - - - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl
Alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl
Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Micro
Crystallin 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Squirrel 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan
Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl
acetate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 6 : Anti-inflammatory Effects of Cosmetic Composition Containing Yunni Tree Extract (Ear Edema Test)

The anti-inflammatory effect was measured using the cosmetic composition containing the Yunri tree extract according to Examples 1 to 7 of Table 6 and the cosmetic composition of the comparative example.

Eighteen healthy mice were studied, divided into three groups per experimental group, and two animals were tested per group. Both ears of two rats in one group were wiped well with alcohol (ethanol), and then one rat was applied with the test group and one rat with 4 μl of the control group (purified water). After 1 hour, 20 µl of acetone was applied to the left ear of both rats in one group, and arachidonic acid, an inflammation-positive control material, was applied to the right ear. After 1 hour again, the thickness of both ears was repeatedly measured using a micrometer to obtain an average value, and the anti-inflammatory effect was calculated according to Equation 6 below. The results are shown in Table 7.

A = inflated thickness in the experimental group

B = inflated thickness in the control

product Swelling Anti-inflammatory effect Increased thickness Difference from control Example 1 1.88 0.99 34.50% Example 2 1.77 1.10 38.32% Example 3 1.77 1.10 38.32% Example 4 1.48 1.39 48.43% Example 5 1.68 1.19 41.46% Example 6 1.87 1.00 34.84% Example 7 1.74 1.07 37.28% Comparative Example 1 2.84 0.03 1.04% Control (purified water) 2.87 - -

Through the results of the measurement of the anti-inflammatory effect of Table 7, the cosmetic composition of Example 1 to Example 7 containing the yunniri extract of the present invention, a comparison comprising glycerin and 1,3 butylene glycol in place of the yunniri extract Compared with the cosmetic composition of Example 1, it can be seen that the excellent anti-inflammatory effect.

Experimental Example  7: Yun-ri tree  Efficacy of Skin Extracts

In order to determine the skin irritation of the cosmetic composition containing the extract of the present invention, Example 1 to 3, Comparative Example 1 and control in 5 × 20 cm in the lower part of both arms of the healthy adult male and female After adjusting the pH to 5.5 with 0.3% lactic acid aqueous solution as an irritant to 100 parts by weight of the cosmetic, respectively, about 0.1 g was applied for 24 hours, and then removed, and after 1 hour and 24 hours had elapsed, The skin condition change was read visually, and the stimulation rate (%) was calculated according to the following equation. As a comparative example, 0.3% lactic acid aqueous solution (v / w) was used. The experimental results are shown in Table 8 below.

Criteria

-: No erythema or unusual phenomenon

+ -: slightly reddish from the surroundings

+: Significantly reddened than surrounding

++: Severe reddening and swelling around

sample Judgment result Stimulation rate
(%) ++ + + - - Example 1 + lactic acid 0.3% - 4 6 20 15.5% Example 2 + 0.3% lactic acid - 2 4 24 8.89% Example 3 + lactic acid 0.3% One 5 24 7.78% Example 4 + lactic acid 0.3% 2 4 24 8.89% Example 5 + lactic acid 0.3% 2 3 25 7.77% Example 6 + lactic acid 0.3% 2 4 24 8.89% Example 7 + lactic acid 0.3% 2 4 24 8.89% Comparative Example 1 + lactic acid 0.3% 3 15 11 One 56.66% Control Example + Lactic Acid 0.3% 7 15 8 - 65.55%

As can be seen from the skin irritation relaxation test results of Table 8, the cosmetic composition (Examples 1 to 7) containing the Yunri tree extract of the present invention is a control and a comparison comprising glycerin and 1,3 butylene glycol or sorbitol Compared with Example 1, it can be seen that the stimulation rate caused by the skin stimulation source decreases.

Experimental Example  8 : Yun-ri tree  Moisturizing effect of extract

Clinical moisturizing effect on the cosmetic composition of Examples 1 to 7 was measured as follows. Through a survey, 125 healthy adult men and women who feel dry skin were randomly divided into 5 groups (A, B, C, D, and E) of 25 persons, and the groups A, B, and C were used in Examples 1-7. The cosmetic composition, in the D group, each of the cosmetic composition containing glycerin and 1,3 butylene glycol as a moisturizing agent (Comparative Example 1), instead of the Yunni-tree extract, The cosmetic composition was applied to the face twice a day for 8 weeks. The moisturizing effect was evaluated using Corneometer CM 820 (Corage + Kazaka, Germany) every two weeks from the start of the practical test and after the end. The experimental results are shown in Table 9 below.

Skin Moisturizing Effect of Yunni Tree Extract division Before use 2 weeks after use 4 weeks after use Example 1 23.5 38.6 45.7 Example 2 23.2 36.4 43.6 Example 3 23.2 37.8 47.4 Example 4 23.2 37.8 45.4 Example 5 23.5 38.6 42.7 Example 6 23.2 36.4 39.6 Example 7 23.5 38.6 44.7 Comparative Example 1 24.5 35.9 40.8 Comparative Example 2 22.8 36.5 37.9

As shown in Table 9, it can be confirmed that the moisturizing effect of the cosmetics containing the Yunri tree extract of the present invention (Examples 1 to 7) is excellent even when compared to cosmetics containing other general moisturizing agents (Comparative Examples 1 and 2). there was.

Experimental Example  9. Yun-ri tree  Effect of extracts on skin wrinkles

A clinical experiment was carried out on the skin wrinkle improvement effect of the cosmetics containing the extract of Yunni tree of the present invention. Clinical trials were evaluated through the actual use test of each cosmetic.

That is, the clinical experiment is the cosmetic composition of Example 2 containing the Yunri tree extract of Preparation Example 2 of Table 6, the cosmetic composition of Comparative Example 1 containing glycerin and 1,3 butylene glycol as a moisturizer instead of the Yunri tree extract Using the composition and the cosmetic composition of Comparative Example 3 containing adenosine known to have an anti-wrinkle effect in place of the extract of Yunri tree, the effect of improving the wrinkles of the skin caused by the use of the cosmetic was confirmed and compared.

Experimenter (20-35 year old female) A group of 20 women, each of which consisted of 20 cosmetic groups containing glycerin and 1,3 butylene glycol as moisturizers instead of Yunri tree extract (Comparative Example 1), treatment group, Yunnori The cream extract (Comparative Example 3), in which the tree extract was replaced with adenosine 5.0% (v / v), was divided into three groups of the experimental group of Example 2 including the Yunri tree extract and wrinkles after 6 weeks using both sides of the face. The improvement effect was visually evaluated to confirm the degree of wrinkle improvement. The results of the wrinkle improvement effect based on this evaluation are shown in Table 10 below.

Wrinkle improvement effect of cosmetic composition containing Yunni extract (visual evaluation) Wrinkle improvement effect Effective rate (%) Great slightly none Example 2
(Yunri tree extract) 24 4 2 85.0 Comparative Example 1
(Moisturizer) 3 4 23 25.0 Comparative Example 3
(Adenosine) 17 7 6 80.0

As can be seen from the results of Table 10, the cosmetic composition of Example 2 containing the extract of the present invention of the present invention showed a wrinkle improvement effect about 3.4 times higher than that of Comparative Example 1 containing a moisturizing agent.

In addition, the result was superior to the cosmetic of Comparative Example 3 containing adenosine known to have an anti-wrinkle effect instead of the Yun-ri extract, indicating that the excellent extract of the yunri extract.

Experimental Example  10: Yun-ri tree  Antimicrobial Effects of Extracts

In order to examine the antimicrobial effect of Yunni-tree extract, the cosmetic composition of Example 8-18, which contains the Yunni-tree extract of Preparation Examples 1 to 4 in various contents in the composition of Table 11, and methylparaben as an antiseptic instead of Yunni-tree extract Control cosmetics containing purified water were prepared instead of the cosmetics of Comparative Example 4 containing the imidazolidinylurethane and the extract of Yunri.

ingredient Example Comparative example Control group 8 9 10 11 12 13 14 15 16 17 18 4 Content (unit: weight%) Production Example 1 0.5 1.0 2.0 - - - - - - - - - - Production Example 2 - - - - - - 0.5 1.0 2.0 - - - - Production Example 3 - - - - - - - - - 0.5 1.0 - - Production Example 4 - - - 0.5 1.0 2.0 - - - - - - - Methylparaben - - - - - - - - - - - 0.2 - Imidazolidinyl
Urea - - - - - - - - - - - 0.2 - glycerin 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Polysorbate 60 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Glyceryl
Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Wax 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Macadamia Nut Oil 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100

The antimicrobial effect on bacteria and fungi was measured using the cosmetics of Examples and Comparative Examples of Table 11.

First, in order to measure the antimicrobial activity against bacteria, 20-30 g of the cosmetics of Examples 8 to 18 and the cosmetics of Comparative Example 4 and the control group Escherichia coli ( Esherichia coli ; ATCC 8739), Pseudomonas aeruginosa (ATCC9027) and Staphylococcus aureus (ATCC6538) were mixed and added so that the initial concentration per sample was 10 ⁷ cfu / g. 1 g of each cosmetic was taken at 1, 7, 14, 21 and 28 days intervals while culturing them in a thermostat at 30-32 ° C for 4 weeks, and the number of viable cells was measured.

In addition, Candida albicans (ATCC10231) and Penicillium cytrinum ( Penicillium ) were used to measure the antimicrobial effect against fungi. citrinum ; KCTC2123) and Aspergillus niger (ATCC16404) were added and mixed at 10 ⁷ cfu / g per sample, followed by incubation in a 25 ° C incubator at 7 days intervals and mycelia and spores on the sample surface. Observation of the occurrence and the results are shown in Table 12 below.

Cosmetics Bacteria (cfu / g) mold* Initial number of bacteria Day 1 Day 7 Day 14 Day 21 Day 28 Example 8 1 x 10 ⁷ 45000 45000 2000 1000 <500 - Example 9 1 x 10 ⁷ 44000 2000 1000 500 <200 - Example 10 1 x 10 ⁷ 45000 1000 500 <100 <100 - Example 11 1 x 10 ⁷ 15000 <100 <100 <100 <100 - Example 12 1 x 10 ⁷ 15000 <100 <100 <100 <100 - Example 13 1 x 10 ⁷ 15000 <100 <100 <100 <100 - Example 14 1 x 10 ⁷ 41000 500 250 <250 <250 - Example 15 1 x 10 ⁷ 45000 300 200 200 <200 - Example 16 1 x 10 ⁷ 44000 150 <100 <100 <100 - Example 17 1 x 10 ⁷ 45000 <100 <100 <100 <100 - Example 18 1 x 10 ⁷ 45000 <100 <100 <100 <100 Comparative Example 4 1 x 10 ⁷ 40000 300 <100 <100 <100 - Control group 1 x 10 ⁷ 54000000 30000000 > 1x10 ⁸ > 1x10 ⁸ > 1x10 ⁸ +++

-: No spawning and mycelium spawning for 8 weeks and good

+: Molding on the wall or lid within 4 weeks

++: mending within 4 weeks and mold on part of the surface

+++: Odor and mold on entire surface within 4 weeks

As shown in Table 12, the cosmetic composition of the control group without preservatives can be found that within 4 weeks of deodorization and mold on the entire surface, the bacterial bacteria significantly increased even after one day. However, the cosmetic composition of Example 8 to Example 18 containing the extract of Yunri tree of the present invention showed a concentration-dependent antimicrobial effect against bacteria and fungi, and after about 28 days, 2.0% (w / v) When used as a content of the preservatives similar to or better than the cosmetics (comparative example 4) mixed with a methyl paraben preservative and imidazolidinyl urea, the antiseptic power of the present invention was determined even when judged as a whole culture day When the extract was used in an amount of 2.0% or more, it was found that much better antiseptic power was secured compared to the cosmetics (comparative example 4) mixed with the synthetic preservative methylparaben and imidazolidinyl urea.

In particular, in the case of Examples 11 to 13, which is a cosmetic containing a fermented extract of Yunni yeast (Preparation Example 4), a strong antimicrobial effect was confirmed from day 1. From this fact, it can be seen that the yunniri extract of the present invention is superior to the antibacterial activity of the fermented extract than the extract obtained by the general solvent extraction method.

Based on the experimental example, the cosmetic composition of various solubilized formulations and emulsion formulations containing Yunni tree extract is presented. Although this is described in detail in the following formulation example, the composition of the present invention is not limited only to the following examples.

Formulation Example  One

Formulation examples of the softening longevity in the cosmetic containing the extract of Yunni wood of Preparation Example 1 are as shown in Table 13.

Softening longevity ingredient Content (% by weight) Cypress Tree Extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example  2

Formulation example of the nutrient cosmetics in the cosmetic containing the extract of Yunni wood of Preparation Example 1 is shown in Table 14.

Nutrition ingredient Content (% by weight) Cypress Tree Extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example  3

Formulation example of the nourishing cream in the cosmetic containing the extract of Yunni wood of Preparation Example 1 is shown in Table 15 below.

Nutrition Cream ingredient Formulation Example Content (% by weight) Cypress Tree Extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Wax 2.6 Tocopheryl acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example  4

Formulation example of the massage cream in the cosmetic containing the extract of Yunni wood of Preparation Example 1 is shown in Table 16 below.

Massage cream ingredient Content (% by weight) Cypress Tree Extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example  5

Formulation examples of the pack in the cosmetic containing the extract of Yunri tree of Preparation Example 1 are as shown in Table 17.

pack ingredient Content (% by weight) Cypress Tree Extract 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glycerin 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Balance system 100

Formulation Example  6

Formulation examples of the essence in the cosmetic containing the extract of Yunri tree of Preparation Example 1 are as shown in Table 18.

essence ingredient Content (% by weight) Cypress Tree Extract 0.1 glycerin 10.0 Betaine 5.0 PEG-1500 2.0 Allantoin 0.1 DL- Panthenol 0.3 EDTA 0.02 Benzophenone-9  0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl Polymer  0.2 Triethanolamine  0.18 Octyldodec-16  0.4 Octyldodecanol  0.3 ethanol 6.0 Preservatives, flavorings, pigments  a very small amount Distilled water Balance system 100

Formulation Example   7

Formulation example of the soap in the cosmetic composition containing the extract of Yunri tree of Preparation Example 1 is as shown in Table 19.

soap ingredient Content (% by weight) Cypress Tree Extract 0.5 Palm oil fatty acid 1.5 Titanium dioxide 0.5 glycerin 0.5 EDTA-2Na 0.15 Spices 0.7 Pigment  Suitable amount Soap chip base
(Contains 15 wt% of moisture) Balance system 100

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (10)

Cosmetic composition for improving skin wrinkles containing Yunni extract as an active ingredient. Cosmetic composition for skin whitening containing Yunni extract as an active ingredient. Moisturizing cosmetic composition containing Yunni extract as an active ingredient. Cosmetic composition for alleviating skin irritation containing Yunni extract as an active ingredient. The cosmetic composition according to any one of claims 1 to 4, wherein the extract of Yunni-tree is contained in an amount of 0.001 to 30.0 wt% based on the total weight of the cosmetic composition. The method according to any one of claims 1 to 4, wherein the yunni extract is (a) water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl Solvent extraction using an extraction solvent selected from the group consisting of acetate, chloroform, diethyl ether, dichloromethane, hexane and mixtures thereof, (b) ultrasonic extraction, (c) supercritical extraction, (d) fermentation or (e) It is obtained by the foaming method, The cosmetic composition characterized by the above-mentioned. delete delete delete delete