JPS60256464A - Membrane type serum sampling system and method - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed Description of the Invention] Technical Field> The present invention uses a model plasma separator to separate and collect only plasma from blood when blood is collected from a donor, and at the same time returns plasma-poor blood to the donor. The present invention relates to a model sample collection system and molded sample collection method.

<Prior art> Conventionally, as a plasma sampling method, there is a plasma sampling method (method 1) in which plasma is separated and collected from extracorporeal blood using a centrifuge. However, it is uneconomical because it requires expensive equipment. The amount of extracorporeally circulating blood during plasma collection is large, the long time it takes to collect and return blood, and there are concerns about side effects such as chills, shivering, and hypotension. blood cell fragments,
It has problems such as the fact that it tends to contain impurities in the throat.

<Purpose>') The purpose of the present invention is to provide a model plasma collection system and a molded plasma collection method that solve the above problems by adopting a method in which plasma collection and blood return are performed simultaneously in the membrane separation method. According to the present invention, the advantages are that it is highly economical, the amount of extracorporeal circulating blood during plasma collection is small, the plasma collection and blood return time is short, there are no side effects, and pure separated plasma can be obtained. has.

<Example> An embodiment of the system of the present invention will be described based on the drawings.

Figures 1 and 2 show the flow path system of the model plasma sampling system of the present invention. blood sampling indwelling needle,
An anticoagulant mixing unit that adds an anticoagulant to blood that has flowed out of the body to make anticoagulated blood, a model plasma separator that separates plasma from the anticoagulated blood, and a model plasma separator that A plasma container that collects plasma separated from blood, a blood return indwelling needle that punctures another vein and withdraws plasma-poor blood from the body, and a plasma infusion device that introduces plasma from a model plasma separator into a plasma container. The device is characterized by a blood circuit that communicates with a blood collection indwelling needle, an anticoagulant mixer, a model plasma separator, and a blood return indwelling needle in this order.

The blood collection indwelling needle used in the present invention punctures a vein and blood flows out of the body at a flow rate of 10 to 100 tttl/min, and a metal or plastic indwelling needle or a winged needle can be used. .

The anticoagulant mixing unit used in the present invention adds an appropriate amount of anticoagulants such as sodium citrate solution, heparin, prostaglandin, gabexate mesylate, and antithrombin to the blood that has flowed out of the body. For example, as shown in Figure 2, there is an anticoagulant introduction pump that introduces and adds a required flow rate of anticoagulant to the blood, and an anticoagulant introduction pump that introduces and adds the anticoagulant to the blood that flows out of the body and the added anticoagulant. It consists of a mixer that mixes anticoagulated blood with blood agents and introduces it as anticoagulated blood into a model plasma separator.The mixer monitors the flow rate of anticoagulated blood and, if necessary, performs anticoagulation to remove anticoagulated blood. The flow rate of the coagulant introduction pump can be adjusted.

The model plasma separator used in the present invention separates plasma from anticoagulated blood by membrane separation, and membrane materials include semi-synthetic polymer membranes such as cellulose diacetate membranes and cellulose triacetate membranes, and polyolefin membranes. film,
Examples include synthetic polymer membranes such as polyacrylate membranes, polyvinyl membranes, polycarbonate membranes, and polysulfone membranes.
Polymer membranes with membrane pore sizes of 0.1-1 microns are preferred. Examples of the membrane shape include a hollow base type, a flat membrane type, and a tube type, with a hollow fiber type being particularly suitable.

Plasma separated from blood in the model plasma separator is collected in a plasma container. As the plasma container, a blood bag type container (for example, made of ethylene-vinyl acetate copolymer sheet) is suitable and is convenient to handle.

The blood return indwelling needle used in the present invention punctures another vein to directly return plasma-poor blood into the body, and can be a metal or plastic indwelling needle or a winged needle.

The plasma introduction path used in the present invention introduces separated plasma from the plasma outlet of the model plasma separator into the plasma container,
Made of soft plastic tube as king. If necessary, a plasma pump can be placed in the middle of the plasma introduction path, and the plasma flow force and separation membrane pressure can be adjusted.

The blood circuit used in the present invention is connected in this order to a blood collection indwelling needle, an anticoagulant mixer, a model plasma separator, and a blood return indwelling needle, and consists mainly of a soft plastic tube.

In the present invention, as shown in FIG. 2, a replenisher pump and, if necessary, a replenisher warmer are installed between the membrane di-type plasma separator and the blood return indwelling needle to control the plasma flow rate in the plasma introduction path. It is also possible to lower the viscosity of plasma-poor blood by providing a replenisher introduction section that can introduce and mix physiological saline at a flow rate of 0 to 0.5 times into plasma-poor blood.

In addition, in the membrane-type plasma sampling method of the present invention, a blood collection indwelling needle is punctured into a vein to drain blood out of the body, and an anticoagulant is added and mixed with the blood in an anticoagulant mixing section to prevent anticoagulation. As blood,
Anticoagulated blood flows into a model plasma separator to separate plasma and collect it in a plasma container, and then the plasma-poor blood flowing out from the model plasma separator is added to the flow rate of the separated plasma from 0 to 0,5
It is characterized by introducing and mixing double the flow rate of physiological saline, and then returning plasma-poor blood to the body through a blood return indwelling needle punctured into another vein.

Next, the method of the present invention will be explained using examples.

In the method of the present invention, blood from a blood donor's vein flows into the blood circuit through a blood collection indwelling needle at a blood flow rate of 45 cm. On the other hand, the anticoagulant (for example, sodium citrate solution) is removed from the anticoagulant container by -'1
The anticoagulant is introduced into the blood circuit at a flow rate of 5 πt/min by an anticoagulant introduction pump, mixed in a mixer, and the anticoagulated blood is transferred to a cellulose triacetate hollow fiber membrane at a flow rate of 50 m/'/min. type plasma separator (effective membrane area 0.25 rr
? ). In the plasma separator, the average plasma flow rate 1
8 separates plasma in 7 minutes, and the separated plasma is collected in a plasma container made of ethylene-vinyl acetate copolymer sheet through a plasma introduction channel. - The plasma-poor blood (red blood cell concentrate) flowing out of the plasma separator is mixed with physiological saline at a flow rate of 0 to 0.5 times the flow rate of the separated plasma, for example, an average of 6.4 tttlZ, and then The blood is returned to the body through a blood return indwelling needle inserted into another vein.

In this way, 500 ml of plasma could be collected after 30 minutes of blood collection and simultaneous blood return. In the method of the present invention, the amount of extracorporeally circulating blood was 150 g/. No side effects such as chills, shivering, or hypotension were observed during the sample collection. Furthermore, the separated plasma did not contain any impurities such as platelets or blood cell fragments.

Effect〉 The effects of the present invention are shown below.

(1) Since the mechanism is simplified, it is highly economical.

(2) Since the membrane-type blood drug separator is small and uses a method of returning blood at the same time as plasma collection, the amount of extracorporeally circulating blood during plasma collection is small.

(3) Since the amount of extracorporeally circulating blood during plasma collection is small and a closed circuit system is used, no side effects occur.

(4) Since membrane separation prevents permeation of formed components, pure separated plasma can be obtained.

(5) The model sample sampling system is small and easy to handle.

[Brief explanation of drawings]

FIG. 1 and FIG. 2 show a flow path system of an embodiment of the model serum sampling system of the present invention. Patent applicant Nissho Co., Ltd. −

Claims (5)

[Claims] (1) A blood collection indwelling needle that punctures a vein and drains blood out of the body, an anticoagulant mixing unit that adds an anticoagulant to the blood that has drained out of the body to make anticoagulated blood, and the anticoagulant. A model plasma separator that separates plasma from converted blood, a plasma container that collects the plasma separated from blood by the model plasma separator, and a blood return device that returns plasma-poor blood to the body by puncturing another vein. Consists of an indwelling needle, a plasma introduction channel that introduces plasma from the model plasma separator to the plasma container, and a blood circuit that communicates with the blood collection indwelling needle, anticoagulant mixer, model plasma separator, and blood return indwelling needle in this order. A model serum collection system featuring this feature. (2) The anticoagulant mixing unit includes an anticoagulant introduction pump that introduces and adds a required flow rate of anticoagulant to the blood, and an anticoagulant mixing unit that mixes the blood that has flowed out of the body and the added anticoagulant. It consists of a mixer that introduces anticoagulated blood into a model plasma separator, in which the flow rate of anticoagulated blood is monitored and, if necessary, anticoagulated blood is carried out to adjust the flow rate of the anticoagulant introduction pump. 2. The model sample sampling system according to claim 1, wherein the model sample sampling system is configured to adjust: (3) The model plasma collection system according to claim 1, wherein the model plasma separator is made of a polymer membrane having a membrane pore diameter of 0.1 to 1 micron. (4) Between the model plasma separator and the blood return indwelling needle, it consists of a replenisher pump and, if necessary, a replenisher warmer, and physiological saline at a flow rate of 0 to 0.5 times the plasma flow rate in the plasma introduction channel. 2. The model plasma collection system according to claim 1, further comprising a replenisher introduction section that can introduce and mix water with the plasma-poor blood. (5) Puncture a blood collection indwelling needle into a vein to allow blood to flow out of the body, add and mix an anticoagulant to this blood in an anticoagulant mixing section to make anticoagulated blood, and use a model plasma separator. anticoagulated blood flows into the plasma separator, plasma is separated and collected in a plasma container, and then the plasma-poor blood flowing out from the model plasma separator is divided into O-0.5 times the flow rate of the separated plasma. A molded serum collection method characterized by introducing and mixing physiological saline, and then returning plasma-poor blood into the body through a blood return indwelling needle punctured in another vein.