JPS5894386A - Deodorization of abyssal fish oil - Google Patents

Abstract

PURPOSE:To remove the characteristic fishy smell of an abyssal fish oil, with simple method, by treating the oil with soil and a nutrient medium for the proliferation of microorganisms. CONSTITUTION:Soil (ordinary soil of paddy field, plowed land, forestry, etc.) and a nutrient medium for the proliferation of microorganisms (e.g. Waksman medium, bouillon medium, etc.) are added to an abyssal fish oil (e.g. orange raffy oil containing 88% 36-42C ester and 7.6% triglyceride), and mixed by shaking or agitation. The deodorized oil is separated and recovered by centrifugal separation, filtration, etc. The effect can be further improved by inoculating soil microorganisms in the oil.

Description

Detailed Description of the Invention The present invention is characterized in that oil obtained from deep-sea fish is treated with soil and a nutrient medium for propagating microorganisms, soil and a nutrient medium for propagating microorganisms, and soil microorganisms or a culture solution of soil microorganisms. It concerns a method for deodorizing deep-sea fish oil.

Traditionally, natural oils have been widely used as base materials for cosmetics, but
Among these, fish oil has a fishy odor unique to fish, so in reality it is hardly used as a raw material for cosmetics compared to vegetable oils. This is due to the superior properties of fish oil. This is because no deodorizing method has been found, and if this method is developed, it is expected that it will be used dramatically as a cosmetic oil.

Conventionally, various studies have been made for this purpose.

For example, the best method is to harden fish oil, but this method changes the melting point and is not preferred when used as a cosmetic oil.

The present inventors have found that when fish oil is treated with soil, a nutrient medium for microbial propagation, soil microorganisms, or a culture solution of soil microorganisms,
Among fish oils, it has been found that this method is particularly effective for deep-sea fish oil, and that the fishy odor can be efficiently removed while retaining the characteristics of the oil, leading to the completion of the present invention.

That is, the present invention provides (1) a method for deodorizing deep-sea fish oil, which is characterized by treating deep-sea fish oil with soil and a nutrient medium for microbial propagation; and (2) treating deep-sea fish oil with soil, a nutrient medium for microbial propagation, and soil microorganisms. Deep-sea fish oil deodorization method characterized by
(3) The present invention provides a method for deodorizing deep-sea fish oil, which is characterized by treating deep-sea fish oil with a culture solution of soil and microorganisms.

The configuration of the present invention will be explained in detail below.

The deep-sea fish used in the present invention refers to fish that can live deeper than 200 meters below the sea surface, and these fish are adapted to a dark world with great water pressure.

Specifically, orange ranphie, baramura 1 aphid beetle, Japanese horse mackerel, Hikari goldfish, black-and-white bream, black-and-white sea bream, black astragalus, waterfish,
Known species include the black-sea conger, the conger eel, and the coelacanth, and these deep-sea fish are characterized by containing a large amount of wax ester as an oil component.

For example, orencilla and phi are deep-sea fish caught in specific waters near Newland, 1,600 meters below the surface, and have a pale yellow, transparent oil between their skin and flesh.

This oil (hereinafter referred to as orencilla or fee oil) is a pale yellow transparent liquid wax with 88% aged 0311-C42 esters and 7.6% triglycerides.

Also contains some free fatty acids, free alcohols, sterols, and phospholipids.

Among these, phospholipids, glycerides of highly unsaturated fatty acids, and proteins contained as impurities are responsible for the fishy odor characteristic of orensilla and fi oil.

In carrying out the method of the present invention, soil is added to deep sea fish oil and mixed by shaking or stirring.

After that, the deodorized oil is separated and recovered using methods such as centrifugation and filtration.

The soil used in the present invention refers to general soil such as farmland, mountain forest, and garden soil, and these soils generally contain decayed and decomposed products of animals and plants, such as many clay minerals, cellulose, lignin, and protein. Many microorganisms live there.

The amount of soil added in the present invention is Orenshira.

A deodorizing effect is observed if the amount is 10 parts by weight or more based on the weight of the feed oil, but a weight loading of 50 parts by weight or more is preferable to obtain orensilla and feed oils that do not have a fishy odor.

The soil used in the present invention is as follows.

Soil A Nippacho mulch Soil B Yokodai garden soil C Yokodai mulch In the present invention, when a known nutrient medium for microbial propagation is further added as a deodorizing treatment agent, the effect is improved.

The nutrient medium for microbial propagation of the present invention includes Tarainski (
Krainskyls) medium, Schiabeck Dox medium, Waxman medium/mineral salt medium, meat juice medium, wort, etc. are selected.

In the present invention, when soil microorganisms are further inoculated as a deodorizing treatment agent, the effect is improved.

The soil microorganisms of the present invention are separated by a conventional method from a soil dispersion liquid in which soil is dispersed in sterilized water using a broth agar medium, potato dextrose agar medium, Tarainski agar medium, etc. A large number of microorganisms such as Actinobacteria, fungi, and Gucchilia are generally isolated.

As described above, the present invention is an excellent method for deodorizing deep-sea fish oil, which can remove the fishy odor unique to deep-sea fish oil in an extremely simple manner.

The present invention will be specifically explained below with reference to Examples.

(Left below) Example 1 Orencilla, fee oil 1009 to soil 21:1g
~50 Riton broth medium, Waxman medium, Shi7pe.

One or more liquid media selected from Ri-Dox medium, wort, inorganic salt medium, and Talline skim medium 100
and shake at 10°C for 1 to 5 days. After shaking and separating (300 Or, p, m) L, the deodorized orencilla and fi oil in the upper layer were collected and the deodorizing effect was investigated, and the results are shown in Table I.

The composition of the medium mentioned in Section 2 is as follows.

Meat juice medium meat extract 10kg Peptone 10ml Salt 59 Water 1000ml, Kussman medium 2HP0. 0.59 M g SO, + 029 NetCI
01g caco3 3. Og yeast extract
0.5g mannitol 10 water 1ooo rnln
lcyacdo, ox medium sucrose 20-309 NaNO339 KHPO'1riK (J O,59 MgSO4・7H200,5g FeSO,0,019 Water 1000 rrl
Wort malt extract 10-50 9 Water 1000 m/
Inorganic salt medium Potassium phosphate 0.5 9 Potassium phosphate 25 9 Magnesium sulfate α3 Calcium chloride 0.3 9 It is effective to get rid of the fishy odor of orencilla and fee oil.

(Left below) Example 2 20 q to 5 liters of soil per 100 liters of orange roughy oil
0 g and broth medium, Waxman medium, and 1 pe.

One or more liquid media selected from Ri-Dox medium, wort, inorganic salt medium, and Kleinski medium 100
Q is added, and one or more soil microorganisms are added to the soil.
Inoculate with a few platinum loops and shake at 1°C for 1 to 5 days.

After shaking, centrifugation (3,000 r, p, m,
) The deodorized orenshiranophy oil in the upper layer was collected and the deodorizing effect was investigated, and the results were as shown in Table ■. It was found that the fishy odor of orencilla and fee oils can be removed by treating them with soil microorganisms.

Table ■ Example 3 Broth medium/Waxmann medium, °Zurbecked.

After sterilizing 100 g of one or more liquid media selected from Kusu medium, wort, inorganic salt medium, and Kleinsky medium, one to several platinum loops of soil microorganisms were inoculated with I'C.
Culture with shaking for 1 to 3 days to allow the bacteria to grow sufficiently. Then add 100 g of orencilla and fi oil and shake at 30' for 1 to 5 days. After shaking, centrifuge (3000r
, p, m,) L,, The deodorized orensilla and fi oil in the upper layer were collected and the deodorizing effect was investigated, and the results were as shown in Table 3.

(The following is a blank space) As shown in Table 4 (■), it was found that by treating orencilla and fee oil with a culture solution of soil microorganisms, the fishy odor peculiar to orencilla and fee oil was removed.

Claims (3)

[Claims] (1) A method for deodorizing deep-sea fish oil, which is characterized by treating deep-sea fish oil with soil and a nutrient medium for breeding microorganisms. (2) A method for deodorizing deep-sea fish oil, which is characterized by treating deep-sea fish oil with soil, a nutrient medium for breeding microorganisms, and soil microorganisms. (3) A method for deodorizing deep-sea fish oil, which is characterized by treating deep-sea fish oil with a culture solution of soil microorganisms.