JPS5894382A - Instrument for cultivation - Google Patents
Instrument for cultivationInfo
- Publication number
- JPS5894382A JPS5894382A JP19305081A JP19305081A JPS5894382A JP S5894382 A JPS5894382 A JP S5894382A JP 19305081 A JP19305081 A JP 19305081A JP 19305081 A JP19305081 A JP 19305081A JP S5894382 A JPS5894382 A JP S5894382A
- Authority
- JP
- Japan
- Prior art keywords
- main body
- elastic material
- elastic
- lid
- cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000005700 microbiome Species 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 6
- 235000015170 shellfish Nutrition 0.000 claims 1
- 239000013013 elastic material Substances 0.000 abstract description 8
- 244000043261 Hevea brasiliensis Species 0.000 abstract 1
- 229920003052 natural elastomer Polymers 0.000 abstract 1
- 229920001194 natural rubber Polymers 0.000 abstract 1
- 229920002379 silicone rubber Polymers 0.000 abstract 1
- 239000004945 silicone rubber Substances 0.000 abstract 1
- 239000000853 adhesive Substances 0.000 description 5
- 230000001070 adhesive effect Effects 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 239000002390 adhesive tape Substances 0.000 description 4
- 210000001520 comb Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
■ 発明の背景
技術分野
本発明は、培養器具に関する。 さらに詳しくは、特に
、微生物ないし細胞の密栓ないし嫌気培養を行うための
培養器具に関する。DETAILED DESCRIPTION OF THE INVENTION ■ Background Technical Field of the Invention The present invention relates to a culture device. More specifically, the present invention particularly relates to a culture device for sealing or anaerobically culturing microorganisms or cells.
先行技術とその問題点
微生物ないし細胞の培養には、開放糸でイ１り培養と、
密栓ないし嫌気培養として密封系でイ’ｒ″）培養とが
ある。。Prior art and its problems For culturing microorganisms or cells, there are two methods: single culture using open threads,
There is culture in a closed system as a hermetically sealed or anaerobic culture.
従来、密封系で１１う培養としては、）＠重用の凹部を
有するシャーレないし、培養用の凹部を：ｋ ＆１有す
る多穴グレート等の本体に、訝体をかぶせ、側面外周に
粘着テープを貼って、すきまを粘着アープで塞いで気密
にすることや、あるいは本体を他の密封できる容器に入
れて気暫にすることが行われている。Conventionally, for culturing in a sealed system, the main body of a Petri dish with recesses for heavy use or a multi-hole grate with recesses for culture of Then, the gap is sealed with adhesive Arp to make it airtight, or the main body is placed in another sealable container and left to air.
しかし、前者のような粘着テープを用いるときには、凹
部内に収納する培地が液体である場合、本体を水平に保
たねば培地がこぼれてしまうため、側面外周に粘着テー
プを貼る作業がむずかしく、熟練を要するという欠点が
ある。 また、後者のように他の容器内に収納する場合
は、作業の簡便さに欠ける。However, when using adhesive tape like the former, if the culture medium stored in the recess is liquid, the culture medium will spill if the main body is not held horizontally, so it is difficult to apply the adhesive tape to the outer periphery of the side surface, and it is difficult to apply it to the outer periphery of the side surface. The disadvantage is that it requires Furthermore, when storing the product in another container as in the latter case, the work is not easy.
これＶこ対し、特に、本体として多穴プレートを用いる
場合、ウェルと称される培養用凹部ないしＪ＠養穴上に
、直接粘着シートを接着し、各ウェル上部を５ｊＭする
ことも行われている。In contrast, when a multi-well plate is used as the main body, an adhesive sheet is directly adhered to the culture recesses or culture holes called wells, and the upper part of each well is 5mm thick. There is.
［７かし、この場合には、ウェル上部に粘着シートか位
置し、ノートの接着剤と培地とが相対するため、培養が
接着剤の影響を受けてしまう危険性がある。 また、ウ
ェル壁上面が濡れていたり、あるいは、上面の幅が狭か
ったりすると、完全な密封が難しく、また培養は高湿榮
件下で行うため、培養中に粘着７−トが一部はがれるこ
ともある。[7] However, in this case, an adhesive sheet is placed above the well, and the adhesive on the notebook faces the medium, so there is a risk that the culture will be affected by the adhesive. In addition, if the top surface of the well wall is wet or the top surface is narrow, it will be difficult to seal it completely, and since the culture is performed under high humidity conditions, some of the adhesive tape may peel off during culture. There is also.
Ｈ発明の目的
不発明は、このような実状に鑑みなされたものであって
、」〕記した欠点ｔＣいし小都合を解消した培養器具を
提供−することを、その王たる目的とする。 より具体
的には、簡易な構造をもち、また′Ｍ到かされめて容易
かつ確実に行えるＪ＠養器具を本発明者は、このよさな
目的につき種々検討をくりかえした結果、本発明をなす
に至った。The object of the invention was made in view of the above-mentioned circumstances, and its main object is to provide a culture device that eliminates the drawbacks and inconveniences mentioned above. More specifically, the inventor of the present invention has developed the present invention, which has a simple structure and which can be easily and reliably performed. I arrived at the eggplant.
すなわち本発明は、微生物ｔ【いし細胞の培養用凹部を
少なくとも一つ有する本体と、該本体を橿う蓋体とを有
し、前記本体の側部外面または蓋体の側部内面に弾性体
を設け、該弾性体を介し、前記本体と前記蓋体とが気密
に係合するよ’５に構成してなることを％徴とする培養
器具である。That is, the present invention has a main body having at least one recess for culturing microorganisms (t) and a lid that covers the main body, and an elastic material is provided on the outer side surface of the main body or the inner side surface of the lid. The culture device is characterized in that it is configured such that the main body and the lid are airtightly engaged with each other via the elastic body.
本発明の実施態様は、上記の目的をより有効に達成する
具体的構造に関するものであり、以下のとおりである。Embodiments of the present invention relate to specific structures that more effectively achieve the above objects, and are as follows.
ｉ）弾性体が、本体の側部外面またはに体の側部内面に
設けられている弾性リングである本発明の培養器具。i) The culture device of the present invention, wherein the elastic body is an elastic ring provided on the outer side surface of the main body or the inner side surface of the body.
１１）弾性リングが、本体の側部外面または蓋体の側部
内面に設けられた少なくとも１つの溝内に着脱可能に嵌
着されている上記ｉ）の培養器具。11) The culture device according to i) above, wherein the elastic ring is removably fitted into at least one groove provided on the outer side surface of the main body or the inner side side surface of the lid.
璽 発明の具体的構成
以下、本発明を第１図〜第３図に示される実施例に従い
、詳細に説明する。DESCRIPTION OF THE PREFERRED EMBODIMENTS Specific Structure of the Invention The present invention will now be described in detail with reference to embodiments shown in FIGS. 1 to 3.
本発明の培５ 器具は、本体１を有する。 本体１は、
培養用の凹部な有するシャーレ状であっても、図示のよ
うに、多数の培養用の凹部２、すなわちウェルを廟する
多穴プレート状であってもよい。 そして、その構造、
形状、材質等は種々のものとすることができる。The culture medium 5 device of the present invention has a main body 1. The main body 1 is
It may be in the shape of a petri dish having a recess for culturing, or as shown in the figure, it may be in the shape of a multi-hole plate having a large number of recesses 2 for culturing, that is, wells. And its structure,
Various shapes, materials, etc. can be used.
本発明の培−＃器具は、この本体１を覆う蓋体４を有す
る。 、ｉ坏４の形状、構造、材質等には特に１ｉｉｌ
Ｊ限はなく、その寸法は、後述の弾性体を介して、蓋体
４が本体１と気密に係合できるものとすればよい。The culture medium ## instrument of the present invention has a lid 4 that covers the main body 1. , especially the shape, structure, material, etc. of i-4.
There is no limit to J, and the dimensions may be such that the lid 4 can be airtightly engaged with the main body 1 via an elastic body, which will be described later.
本体１の側部外面または蓋体の側部内（３）には、弾性
体３が設けられる。An elastic body 3 is provided on the outer surface of the side of the main body 1 or inside the side (3) of the lid.
弾性４３は、徨々の配置にて設けることができるが、最
も簡便には１図示のようＫ、弾性体３を弾性リングとし
、これな本体の側部外ｊｉｊ１５を少なくとも一周する
ように設けるのが好適である。The elastic members 43 can be provided in various arrangements, but the most convenient method is to use an elastic ring as shown in FIG. is suitable.
あるいは、女体４の側部内面に弾性リングを設けること
もできる。Alternatively, an elastic ring may be provided on the inner side of the female body 4.
このように弾性リングを配置し、第３図に示され′るよ
うに、蓋体４を本体１に似合すると、リング状の弾性体
と蓋体４の側部内面とが＠事し、凹部２内部を含む蓋体
１と蓋体４との間か暫封系となる。When the elastic ring is arranged in this way and the lid body 4 is fitted to the main body 1 as shown in FIG. A temporary sealing system is formed between the lid body 1 and the lid body 4 including the inside of the lid body 2.
この場合、弾性リング等の弾性体３は、その材質として
、天然ないし合成コム、シリコンコム等が使用可能であ
るが、これらのうちシリコンコムが最適である。In this case, the elastic body 3 such as an elastic ring can be made of natural or synthetic combs, silicone combs, etc. Among these, silicone combs are most suitable.
一方、弾性リングを本体ｌの１１１１１部外面１５に設
けるには、両者を接着等してもよいか、本俸１の側部外
面１５を少なくとも一周するようにＳ＋Ｌ体３としての
弾性リングが嵌入する隣１５１を少なくとも一つ設け、
この溝１５１内に弾性体３としての弾性リングを着脱可
能に嵌着することか好ましい。 このとき、弾性リン
グは、便用に際し、本体ｌの側部外１１［ｉ１５からず
れることがなく、一方、弾性リンクをとりはずして、開
放系の培養器具としても用いることができるという利点
が生じる。On the other hand, in order to provide the elastic ring on the outer surface 15 of the 11111 part of the main body 1, the two may be bonded together, or the elastic ring as the S+L body 3 may be fitted so as to go around the side outer surface 15 of the main body 1 at least once. Provide at least one neighbor 151,
It is preferable that an elastic ring as the elastic body 3 is removably fitted into the groove 151. At this time, the elastic ring does not shift from the outside side 11 [i15 of the main body l when used for convenience, and on the other hand, there is an advantage that the elastic link can be removed and used as an open culture device.
あるいは、蓋体４の側部内面に溝を設け、この溝内に弾
性リングを着脱可能に嵌入固定することによっても、同
様の利点が生じる。Alternatively, similar advantages can be obtained by providing a groove on the inner surface of the side surface of the lid body 4 and removably fitting and fixing the elastic ring into the groove.
なお、第３図に示される例では、蓋体４の内面の四方の
隅にリプが形成され、上記のように弾性体３をとりはず
して開放系で使用するときの内部、と外部の連通を確保
するための起磁がなされている。In the example shown in FIG. 3, lips are formed at the four corners of the inner surface of the lid body 4, which allows communication between the inside and the outside when the elastic body 3 is removed and used in an open system as described above. Magnetism is being done to ensure this.
■ 発明の具体的作用効果
本発明の培養器具は、凹部２内に培地を収納し、これに
、微生物ないし細胞を入れ、次いで蓋体４を本体１に嵌
着して使用する。(2) Specific Effects of the Invention The culture device of the present invention is used by storing a culture medium in the recess 2, putting microorganisms or cells therein, and then fitting the lid 4 onto the main body 1.
このとき、蓋体４の内面は、弾性体３を介し、本体１側
部外面１５と気密に係合するので、密封性は確実かつ良
好であり、外気の内部への流入はおこらないっ
この場合、Ｊ８女に際し、密封が破れることもな（）。At this time, the inner surface of the lid 4 airtightly engages with the side outer surface 15 of the main body 1 through the elastic body 3, so the sealing performance is reliable and good, and outside air does not flow into the interior. In the case of J8 girls, the seal may break ().
また、４体４を本体１にかぶせこむだけで密封ができ、
封止作業が容易である。In addition, sealing can be achieved by simply placing the 4 parts 4 over the main body 1.
Sealing work is easy.
さらに、それ自体構造が簡易であるうえ、弾性体３を介
して、本体１と′着体４とはつなぎとめられているので
、蓋体４と本体１とを固定する手段は必要なく、この点
からも構造がＴｍｌ＋易となる１゜さらに、粘着テープ
を用い−Ｃ′ｇ封な行うときのような培養への悪影響も
ない。Furthermore, the structure itself is simple, and since the main body 1 and the attached body 4 are connected via the elastic body 3, there is no need for a means for fixing the lid body 4 and the main body 1. Furthermore, there is no adverse effect on the culture unlike when -C'g is sealed using adhesive tape.
この場合、上記実施態様ｉ）にＶ［い、弾性体３として
弾性リングを用いれば、構造がｒＪｌ易となり。In this case, if an elastic ring is used as the elastic body 3 in the above embodiment i), the structure becomes easier.
構造か容易となる。The structure becomes easier.
また、上記実施態様１１）に従い、弾性リングを本体１
の側部外面１５等に形成した溝に１沃１１゛れば、多数
回の使用に際しても溝の脱落はなく、また、着脱可能で
あるので、リングをはずして、開放系の使用を行うこと
もできる。Further, according to the embodiment 11), the elastic ring is attached to the main body 1.
If the grooves formed on the outer side surface 15, etc. of the ring are filled with 1.5 mm, the grooves will not fall off even after multiple uses, and since it is removable, the ring can be removed and used in an open system. You can also do it.
そして、これらの諸効果は、弾性リング等の弾性体を、
蓋体の側部内面に設けても、本体の側部外面に設けても
、同様に実現するものである。These effects make elastic bodies such as elastic rings,
This can be achieved in the same way whether it is provided on the inner surface of the side of the lid or on the outer surface of the side of the main body.
第１図〜第３図は、本発明の実施例を説明するための図
であり、このうち第１図は培養器具な構成する本体の斜
視図、第２図は本体の断面図、第３図は本体と蓋体を組
合せて構成した培養器具の断面図である。
１・・・・・・・・・・・・・・・・・本 体２・・・
・・・−・−・・・・・・・・・凹 部３・・・・・・
・・・・・・・・・・弾性体１５・・・・・・・・・・
・・・・本体側部外面１５１ ・・・・・・・・・・・
溝
４・・・・・・・・・・・・・・蓋体
特許出願人 テルモ株式会社
代理人 弁理士 石 井 陽 −Figures 1 to 3 are diagrams for explaining embodiments of the present invention, of which Figure 1 is a perspective view of a main body constituting a culture device, Figure 2 is a sectional view of the main body, and Figure 3 is a cross-sectional view of the main body. The figure is a sectional view of a culture device constructed by combining a main body and a lid. １・・・・・・・・・・・・・・・・・・ Main body 2...
・・・−・−・・・・・・・・・Concave part 3・・・・・・
・・・・・・・・・Elastic body 15・・・・・・・・・・
...Body side outer surface 151 ......
Groove 4・・・・・・・・・・・・・・・Lid body patent applicant Terumo Corporation representative Patent attorney Yo Ishii −
Claims (1)
つ有する本体と、該本体を覆う蓋体とを有し、 前記本
体の側部外面または蓋体の側部内面に弾性体を設け、
該弾性体を介し、前記本体と前記無体とが気密に係合す
るように構成してなることを特徴とする培養器具。 ２ 弾性体が、本体の側部外向または蓋体の側部内面に
設けら第１ている弾性リングである特許横木の範吐鵡）
２１瑣に記載の培養器具。 ３、 ４τＦリングが、本体の側部外面または蓋体の側
部内面に設けられた少なくとも１つの溝内に盾脱６」能
に嵌着されている特許請求の範囲第２項に記載の培＊！
６貝。[Scope of Claims] ], comprising a main body having at least one recess for culturing microorganisms or cells, and a lid covering the main body; Set up your body,
A culture device characterized in that the main body and the intangible body are configured to airtightly engage with each other via the elastic body. 2. Patent crossbars in which the elastic body is an elastic ring provided on the outer side of the main body or the inner side of the lid body.
The culture device described in 21 (d). 3. The culture medium according to claim 2, wherein the 4τF ring is fitted in at least one groove provided on the outer side surface of the main body or the inner side surface of the lid body in a shield-like manner. *!
6 shellfish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19305081A JPS5894382A (en) | 1981-11-30 | 1981-11-30 | Instrument for cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19305081A JPS5894382A (en) | 1981-11-30 | 1981-11-30 | Instrument for cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5894382A true JPS5894382A (en) | 1983-06-04 |
Family
ID=16301340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19305081A Pending JPS5894382A (en) | 1981-11-30 | 1981-11-30 | Instrument for cultivation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5894382A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010104327A (en) * | 2008-10-31 | 2010-05-13 | Asahi Glass Co Ltd | Container for culture |
| JP2013099280A (en) * | 2011-11-08 | 2013-05-23 | Dainippon Printing Co Ltd | Cell culture container and method for producing the same |
| JP2017536836A (en) * | 2014-12-02 | 2017-12-14 | 陳剣ＣＨＥＮ， Ｊｉａｎ | Electric shock pipe and electroporation apparatus having the electric shock pipe |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5245263U (en) * | 1975-09-26 | 1977-03-30 |
-
1981
- 1981-11-30 JP JP19305081A patent/JPS5894382A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5245263U (en) * | 1975-09-26 | 1977-03-30 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010104327A (en) * | 2008-10-31 | 2010-05-13 | Asahi Glass Co Ltd | Container for culture |
| JP2013099280A (en) * | 2011-11-08 | 2013-05-23 | Dainippon Printing Co Ltd | Cell culture container and method for producing the same |
| JP2017536836A (en) * | 2014-12-02 | 2017-12-14 | 陳剣ＣＨＥＮ， Ｊｉａｎ | Electric shock pipe and electroporation apparatus having the electric shock pipe |
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