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JPS5797451A - Measuring method for enzyme immunity by both sides combination method of proteinic high molecular antigen using regenerable immune adsorption body - Google Patents

Measuring method for enzyme immunity by both sides combination method of proteinic high molecular antigen using regenerable immune adsorption body

Info

Publication number
JPS5797451A
JPS5797451A JP17262780A JP17262780A JPS5797451A JP S5797451 A JPS5797451 A JP S5797451A JP 17262780 A JP17262780 A JP 17262780A JP 17262780 A JP17262780 A JP 17262780A JP S5797451 A JPS5797451 A JP S5797451A
Authority
JP
Japan
Prior art keywords
antigen
antibody
enzyme
high molecular
tagged
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17262780A
Other languages
Japanese (ja)
Inventor
Kanefusa Kato
Akira Kosaka
Ryohei Yamamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Pharmaceutical Co Ltd filed Critical Amano Pharmaceutical Co Ltd
Priority to JP17262780A priority Critical patent/JPS5797451A/en
Publication of JPS5797451A publication Critical patent/JPS5797451A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To reuse a carrier and to measure an antigen accurately, by adsorbing the proteinic high molecular antigen to the insoluble carrier combined an antibody of this antigen tagged by enzyme through S-S bond under the existence of hydrophobic protein and salts. CONSTITUTION:Tagged antibody by enzyme of a proteinic high molecular antigen (alpha-fetoprotein, all sorts of peptid hormones) to be measured in blood serum or urine is combined by S-S bond on the surface of an insoluble carrier such as nylon. After the sample containing said antigen is reacted to said fixing tagged enzyme antibody, the S-S bond is cut by a reducing agent such as thiodislateol and combined body of enzyme-antibody-antigen is eluted and then, the quantity of the antigen in the sample is determined by measuring the enzyme activity in an eluate. On the one hand, the insoluble carrier can be reused by combining the tagged antibody again. Hydrophobic protein such as gelatine and salt of Na, are let coexist in the system to react the antigen-antibody and the accuracy of measurement is improved by restraining the action of interfering substance containing in sample living body fluids.
JP17262780A 1980-12-09 1980-12-09 Measuring method for enzyme immunity by both sides combination method of proteinic high molecular antigen using regenerable immune adsorption body Pending JPS5797451A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17262780A JPS5797451A (en) 1980-12-09 1980-12-09 Measuring method for enzyme immunity by both sides combination method of proteinic high molecular antigen using regenerable immune adsorption body

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17262780A JPS5797451A (en) 1980-12-09 1980-12-09 Measuring method for enzyme immunity by both sides combination method of proteinic high molecular antigen using regenerable immune adsorption body

Publications (1)

Publication Number Publication Date
JPS5797451A true JPS5797451A (en) 1982-06-17

Family

ID=15945378

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17262780A Pending JPS5797451A (en) 1980-12-09 1980-12-09 Measuring method for enzyme immunity by both sides combination method of proteinic high molecular antigen using regenerable immune adsorption body

Country Status (1)

Country Link
JP (1) JPS5797451A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61271458A (en) * 1985-05-28 1986-12-01 Olympus Optical Co Ltd Immunological analysis
WO2015194350A1 (en) * 2014-06-20 2015-12-23 コニカミノルタ株式会社 Sandwich assay using labeled lectin and kit therefor

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61271458A (en) * 1985-05-28 1986-12-01 Olympus Optical Co Ltd Immunological analysis
WO2015194350A1 (en) * 2014-06-20 2015-12-23 コニカミノルタ株式会社 Sandwich assay using labeled lectin and kit therefor
JPWO2015194350A1 (en) * 2014-06-20 2017-04-20 コニカミノルタ株式会社 Sandwich type assay using labeled lectin and kit therefor

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