JPS5788348A - Method and device for spectral fluorescence - Google Patents
Method and device for spectral fluorescenceInfo
- Publication number
- JPS5788348A JPS5788348A JP55163300A JP16330080A JPS5788348A JP S5788348 A JPS5788348 A JP S5788348A JP 55163300 A JP55163300 A JP 55163300A JP 16330080 A JP16330080 A JP 16330080A JP S5788348 A JPS5788348 A JP S5788348A
- Authority
- JP
- Japan
- Prior art keywords
- light
- sample
- fluorescence
- detected
- spectroscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003595 spectral effect Effects 0.000 title abstract 2
- 230000005284 excitation Effects 0.000 abstract 3
- 239000007787 solid Substances 0.000 abstract 3
- 230000035945 sensitivity Effects 0.000 abstract 2
- 230000005540 biological transmission Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000010183 spectrum analysis Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
PURPOSE:To improve spectral sensitivity in a solid sample by using the light from a light source of the direction wherein it does not superpose on light reflected in the sample as light used for spectral analysis. CONSTITUTION:Excitation light from a light source 1 is made into monochromatic excitation light 4 with a spectroscope 3, and when this light 4 is irradiated to a solid sample 11, the sample emits fluorescence 7 of the wavelength specific to the molecules contained in the solid sample. After this fluorescence 7 is split with a spectroscope 8 via a mirror 19, the split light 9 is measured of intensity with a detector 10 for each of wavelengths, the from the distribution in these intensities, the fluorescence is spectrally analyzed and the material is determined. Thereby, the excitation light is detected together with the fluorescence released in the same direction after transmission through the sample, and since the reflection from the sample is not detected, the fluorescence is detected with high sensitivity.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55163300A JPS5788348A (en) | 1980-11-21 | 1980-11-21 | Method and device for spectral fluorescence |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55163300A JPS5788348A (en) | 1980-11-21 | 1980-11-21 | Method and device for spectral fluorescence |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5788348A true JPS5788348A (en) | 1982-06-02 |
Family
ID=15771201
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55163300A Pending JPS5788348A (en) | 1980-11-21 | 1980-11-21 | Method and device for spectral fluorescence |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5788348A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS618649A (en) * | 1984-06-25 | 1986-01-16 | Agency Of Ind Science & Technol | Optical measuring device |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5599047A (en) * | 1979-01-24 | 1980-07-28 | Hitachi Ltd | Measuring apparatus for surface fluorescence |
-
1980
- 1980-11-21 JP JP55163300A patent/JPS5788348A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5599047A (en) * | 1979-01-24 | 1980-07-28 | Hitachi Ltd | Measuring apparatus for surface fluorescence |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS618649A (en) * | 1984-06-25 | 1986-01-16 | Agency Of Ind Science & Technol | Optical measuring device |
JPH0158454B2 (en) * | 1984-06-25 | 1989-12-12 | Kogyo Gijutsuin |
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