JPS5768785A - Activity enhancement of protease - Google Patents
Activity enhancement of proteaseInfo
- Publication number
- JPS5768785A JPS5768785A JP14508380A JP14508380A JPS5768785A JP S5768785 A JPS5768785 A JP S5768785A JP 14508380 A JP14508380 A JP 14508380A JP 14508380 A JP14508380 A JP 14508380A JP S5768785 A JPS5768785 A JP S5768785A
- Authority
- JP
- Japan
- Prior art keywords
- peptide bond
- protease
- formula
- metal protease
- methylamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 4
- 239000004365 Protease Substances 0.000 title abstract 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 4
- 230000000694 effects Effects 0.000 title abstract 2
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 abstract 4
- 229940024606 amino acid Drugs 0.000 abstract 3
- 235000001014 amino acid Nutrition 0.000 abstract 3
- 150000001413 amino acids Chemical class 0.000 abstract 3
- 239000002184 metal Substances 0.000 abstract 3
- 239000012736 aqueous medium Substances 0.000 abstract 2
- 238000005755 formation reaction Methods 0.000 abstract 2
- RTWNYYOXLSILQN-UHFFFAOYSA-N methanediamine Chemical compound NCN RTWNYYOXLSILQN-UHFFFAOYSA-N 0.000 abstract 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 125000000217 alkyl group Chemical group 0.000 abstract 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- 125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 abstract 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 abstract 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 1
- UYWQUFXKFGHYNT-UHFFFAOYSA-N phenylmethyl ester of formic acid Natural products O=COCC1=CC=CC=C1 UYWQUFXKFGHYNT-UHFFFAOYSA-N 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- -1 tryprophan Natural products 0.000 abstract 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To In cutting a peptide bond or carrying out a formation reaction using a metal protease, to enhance the activity of metal protease, by using methylamine or methylenediamin.
CONSTITUTION: For example, in forming amino acids shown by the formulasIand II by the formation of a peptide bond between the amino acid shown by the formula I(Z is benzyloxycarbonyl; Asp is aspartic acid residue) and the amino acid shown by the formula II(X is phenylalanine, tryprophan, tyrosine, leucine, isoleucine residue; R is lower alkyl or amino) or by the cutting of peptide bond shown by the formula III(Z, Asp, X, and R are as shown before) through an enzymatic reaction using a metal protease in an aqueous medium, about 0.5W2.5 mole methylamine or methylenediamine per l aqueous medium is added to the reaction system.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14508380A JPS5768785A (en) | 1980-10-18 | 1980-10-18 | Activity enhancement of protease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14508380A JPS5768785A (en) | 1980-10-18 | 1980-10-18 | Activity enhancement of protease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5768785A true JPS5768785A (en) | 1982-04-27 |
| JPS638748B2 JPS638748B2 (en) | 1988-02-24 |
Family
ID=15376981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14508380A Granted JPS5768785A (en) | 1980-10-18 | 1980-10-18 | Activity enhancement of protease |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5768785A (en) |
-
1980
- 1980-10-18 JP JP14508380A patent/JPS5768785A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS638748B2 (en) | 1988-02-24 |
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