JPS57132884A - Immobilizing method of enzyme - Google Patents
Immobilizing method of enzymeInfo
- Publication number
- JPS57132884A JPS57132884A JP1851781A JP1851781A JPS57132884A JP S57132884 A JPS57132884 A JP S57132884A JP 1851781 A JP1851781 A JP 1851781A JP 1851781 A JP1851781 A JP 1851781A JP S57132884 A JPS57132884 A JP S57132884A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- added
- hydrophobic
- monomers
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 230000003100 immobilizing effect Effects 0.000 title abstract 2
- 239000000178 monomer Substances 0.000 abstract 4
- 230000002209 hydrophobic effect Effects 0.000 abstract 3
- 239000007853 buffer solution Substances 0.000 abstract 2
- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 abstract 1
- OMIGHNLMNHATMP-UHFFFAOYSA-N 2-hydroxyethyl prop-2-enoate Chemical compound OCCOC(=O)C=C OMIGHNLMNHATMP-UHFFFAOYSA-N 0.000 abstract 1
- DBCAQXHNJOFNGC-UHFFFAOYSA-N 4-bromo-1,1,1-trifluorobutane Chemical compound FC(F)(F)CCCBr DBCAQXHNJOFNGC-UHFFFAOYSA-N 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 abstract 1
- 239000002202 Polyethylene glycol Substances 0.000 abstract 1
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Substances CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 abstract 1
- VOZRXNHHFUQHIL-UHFFFAOYSA-N glycidyl methacrylate Chemical compound CC(=C)C(=O)OCC1CO1 VOZRXNHHFUQHIL-UHFFFAOYSA-N 0.000 abstract 1
- 230000005865 ionizing radiation Effects 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 238000010907 mechanical stirring Methods 0.000 abstract 1
- 229920001223 polyethylene glycol Polymers 0.000 abstract 1
- 239000011369 resultant mixture Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 239000007762 w/o emulsion Substances 0.000 abstract 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To immobilize an enzyme at a high immobilizing ratio, by giving a porous structure to a hydrophobic polymeric matrix, and entrapping an enzyme therein.
CONSTITUTION: One or two or more hydrophobic polymerizable monomers polymerizable at 0°C or below, e.g. glycidyl methacrylate or ethylene glycol dimethacrylate, are added to a buffer solution at a suitable pH containing one or two or more enzymes, and if necessary a solvent capable of dissolving both of the buffer solution and the monomers, e.g. polyethylene glycol or an alcohol, is added thereto, or a hydrophilic polymerizable monomer, e.g. hydroxyethyl methacrylate or hydroxyethyl acrylate, is added thereto. The resultant mixture is then mixed thoroughly under mechanical stirring to form a water-in-oil emulsion, which is then irradiated with light or ionizing radiation to polymerize the hydrophobic polymerizable monomers.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1851781A JPS57132884A (en) | 1981-02-10 | 1981-02-10 | Immobilizing method of enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1851781A JPS57132884A (en) | 1981-02-10 | 1981-02-10 | Immobilizing method of enzyme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS57132884A true JPS57132884A (en) | 1982-08-17 |
| JPS6219836B2 JPS6219836B2 (en) | 1987-05-01 |
Family
ID=11973814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1851781A Granted JPS57132884A (en) | 1981-02-10 | 1981-02-10 | Immobilizing method of enzyme |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57132884A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5934886A (en) * | 1982-08-24 | 1984-02-25 | Japan Atom Energy Res Inst | Method for immobilizing enzymes on the surface of porous particles |
| JPS59154988A (en) * | 1983-02-23 | 1984-09-04 | Nitto Electric Ind Co Ltd | Water-dispersible polymer particles and immobilized enzyme using said particles as carrier |
-
1981
- 1981-02-10 JP JP1851781A patent/JPS57132884A/en active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5934886A (en) * | 1982-08-24 | 1984-02-25 | Japan Atom Energy Res Inst | Method for immobilizing enzymes on the surface of porous particles |
| JPH0361426B2 (en) * | 1982-08-24 | 1991-09-19 | Japan Atomic Energy Res Inst | |
| JPS59154988A (en) * | 1983-02-23 | 1984-09-04 | Nitto Electric Ind Co Ltd | Water-dispersible polymer particles and immobilized enzyme using said particles as carrier |
| JPH0229312B2 (en) * | 1983-02-23 | 1990-06-28 | Nitto Denko Corp |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6219836B2 (en) | 1987-05-01 |
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