JPS5712359A - Enzyme electrode - Google Patents
Enzyme electrodeInfo
- Publication number
- JPS5712359A JPS5712359A JP8728780A JP8728780A JPS5712359A JP S5712359 A JPS5712359 A JP S5712359A JP 8728780 A JP8728780 A JP 8728780A JP 8728780 A JP8728780 A JP 8728780A JP S5712359 A JPS5712359 A JP S5712359A
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- enzyme
- insoluble metallic
- metallic complex
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 229940088598 enzyme Drugs 0.000 abstract 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract 2
- 108090000854 Oxidoreductases Proteins 0.000 abstract 2
- 102000004316 Oxidoreductases Human genes 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 abstract 2
- 239000000843 powder Substances 0.000 abstract 2
- 238000004321 preservation Methods 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 108010015776 Glucose oxidase Proteins 0.000 abstract 1
- 239000004366 Glucose oxidase Substances 0.000 abstract 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 229940075397 calomel Drugs 0.000 abstract 1
- 229910052802 copper Inorganic materials 0.000 abstract 1
- 239000010949 copper Substances 0.000 abstract 1
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical compound Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 abstract 1
- 239000003792 electrolyte Substances 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 229940116332 glucose oxidase Drugs 0.000 abstract 1
- 235000019420 glucose oxidase Nutrition 0.000 abstract 1
- 229910002804 graphite Inorganic materials 0.000 abstract 1
- 239000010439 graphite Substances 0.000 abstract 1
- 229910052742 iron Inorganic materials 0.000 abstract 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 abstract 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 229910052697 platinum Inorganic materials 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To obtain an enzyme electrode has superior stability in preservation and speeds and facilitates measurement by fixing oxidoreductase on an insoluble metallic complex and by bringing this complex into electric contact with a conductive substance. CONSTITUTION:On a disk 1 obtained by press-forming oxidoreductase, such as glucose oxidase, insoluble metallic complex, such as iron, copper and manganese, constituting a redox system, and conductive substance (graphite) powder, or by forming and sticking the insoluble metallic complex on the compact after compacting the conductive powder, enzyme 4 is fixed to manufacture an enzyme electrode 5. The electrode 5 is fixed in a cylindrical holder 6 with a screw 7 and a platinum plate current collecting body 8 is contacted and connected to a lead wire 9. Then, the density of a substrate (glucose, etc.) is measured by a device which uses a saturation calomel electrode 10 as a reference electrode, a counter electrode 11 and a phosphoric acid buffer solution 12 as an electrolyte and is equipped with a separator 13. The electrode 5 is stable in preservation and its characteristics are maintained for a long period.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8728780A JPS5712359A (en) | 1980-06-26 | 1980-06-26 | Enzyme electrode |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8728780A JPS5712359A (en) | 1980-06-26 | 1980-06-26 | Enzyme electrode |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5712359A true JPS5712359A (en) | 1982-01-22 |
Family
ID=13910574
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8728780A Pending JPS5712359A (en) | 1980-06-26 | 1980-06-26 | Enzyme electrode |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5712359A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003106966A2 (en) * | 2002-05-02 | 2003-12-24 | Therasense, Inc. | Miniature biological fuel cell that is operational under physiological conditions, and associated devices and methods |
US7811689B2 (en) | 1998-06-17 | 2010-10-12 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US8206563B2 (en) | 2001-05-16 | 2012-06-26 | Abbott Diabetes Care Inc. | Device for the determination of glycated hemoglobin |
US9668684B2 (en) | 2009-02-26 | 2017-06-06 | Abbott Diabetes Care Inc. | Self-powered analyte sensor |
-
1980
- 1980-06-26 JP JP8728780A patent/JPS5712359A/en active Pending
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8435682B2 (en) | 1998-06-17 | 2013-05-07 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US8679688B2 (en) | 1998-06-17 | 2014-03-25 | Abbott Diabetes Care In. | Biological fuel cell and methods |
US9509010B2 (en) | 1998-06-17 | 2016-11-29 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US7811689B2 (en) | 1998-06-17 | 2010-10-12 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US7998625B2 (en) | 1998-06-17 | 2011-08-16 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US7998624B2 (en) | 1998-06-17 | 2011-08-16 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US9070934B2 (en) | 1998-06-17 | 2015-06-30 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US8241797B2 (en) | 1998-06-17 | 2012-08-14 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
USRE45370E1 (en) | 1998-06-17 | 2015-02-10 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US8889305B2 (en) | 1998-06-17 | 2014-11-18 | Abbott Diabetes Care Inc. | Biological fuel cell and methods |
US8460525B2 (en) | 2001-05-16 | 2013-06-11 | Abbott Diabetes Care Inc. | Device for the determination of glycated hemoglobin |
US8206563B2 (en) | 2001-05-16 | 2012-06-26 | Abbott Diabetes Care Inc. | Device for the determination of glycated hemoglobin |
US8759055B2 (en) | 2002-05-02 | 2014-06-24 | Abbott Diabetes Care Inc. | Miniature biological fuel cell that is operational under physiological conditions, and associated devices and methods |
WO2003106966A2 (en) * | 2002-05-02 | 2003-12-24 | Therasense, Inc. | Miniature biological fuel cell that is operational under physiological conditions, and associated devices and methods |
WO2003106966A3 (en) * | 2002-05-02 | 2004-07-01 | Therasense Inc | Miniature biological fuel cell that is operational under physiological conditions, and associated devices and methods |
US7368190B2 (en) | 2002-05-02 | 2008-05-06 | Abbott Diabetes Care Inc. | Miniature biological fuel cell that is operational under physiological conditions, and associated devices and methods |
US9668684B2 (en) | 2009-02-26 | 2017-06-06 | Abbott Diabetes Care Inc. | Self-powered analyte sensor |
US10631768B2 (en) | 2009-02-26 | 2020-04-28 | Abbott Diabetes Inc. | Self-powered analyte sensor |
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