JPS5655853A - Quantitative determination of amino acid - Google Patents
Quantitative determination of amino acidInfo
- Publication number
- JPS5655853A JPS5655853A JP13229279A JP13229279A JPS5655853A JP S5655853 A JPS5655853 A JP S5655853A JP 13229279 A JP13229279 A JP 13229279A JP 13229279 A JP13229279 A JP 13229279A JP S5655853 A JPS5655853 A JP S5655853A
- Authority
- JP
- Japan
- Prior art keywords
- ninhydrin
- reagent
- amino acid
- crystallization
- sedimentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
PURPOSE: To eliminate the scattering of measured values, by employing a reagent composition containing ninhydrin in the quantity within specified limits and thereby preventing the crystallization and sedimentation in the liquid-sending tube of an automatic amino acid analyzer, as well as the turbidily formation and the change in the liquid flow rate in an absorbance-measuring system.
CONSTITUTION: The quantity of ninhydrin in the ninhydrin reagent used for analysis of amino acids is set to be 0.5W1.5% (weight/volume) which is less than that in the conventional one. In this way, the crystallization and sedimentation caused by the combination of ninhydrin and hydrinduntin formed by the reduction of the former with a reducing agent present in the reagent are prevented. By setting the quantity of ninhydrin within the limits as mentioned above, the composition whose analytical sensitivity (strength of coloration) is not changed and which has excellent stability with the passage of time can be obtained.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13229279A JPS5655853A (en) | 1979-10-13 | 1979-10-13 | Quantitative determination of amino acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13229279A JPS5655853A (en) | 1979-10-13 | 1979-10-13 | Quantitative determination of amino acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5655853A true JPS5655853A (en) | 1981-05-16 |
Family
ID=15077870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13229279A Pending JPS5655853A (en) | 1979-10-13 | 1979-10-13 | Quantitative determination of amino acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5655853A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015536464A (en) * | 2012-11-21 | 2015-12-21 | ジェイピーピー クロマトグラフィー リミテッド | A novel ninhydrin reagent for the analysis of nitrogen-containing compounds |
-
1979
- 1979-10-13 JP JP13229279A patent/JPS5655853A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015536464A (en) * | 2012-11-21 | 2015-12-21 | ジェイピーピー クロマトグラフィー リミテッド | A novel ninhydrin reagent for the analysis of nitrogen-containing compounds |
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