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JPS55101052A - Simple measuring method for hemoglobin glucoside in blood - Google Patents

Simple measuring method for hemoglobin glucoside in blood

Info

Publication number
JPS55101052A
JPS55101052A JP707379A JP707379A JPS55101052A JP S55101052 A JPS55101052 A JP S55101052A JP 707379 A JP707379 A JP 707379A JP 707379 A JP707379 A JP 707379A JP S55101052 A JPS55101052 A JP S55101052A
Authority
JP
Japan
Prior art keywords
ion
buffer liquid
exchange resin
glucoside
hemoglobin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP707379A
Other languages
Japanese (ja)
Inventor
Shoji Maruyama
Hiroshi Igarashi
Eiko Kayane
Toshisada Ishido
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Original Assignee
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SOGO SEIBUTSU IGAKU KENKYUSHO KK filed Critical SOGO SEIBUTSU IGAKU KENKYUSHO KK
Priority to JP707379A priority Critical patent/JPS55101052A/en
Publication of JPS55101052A publication Critical patent/JPS55101052A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE: To make it possible to take an accurate measurement in a short time by using a small quantity of ion-exchange resin by measuring the absorbance of a separated liquid layer after adding hemolysis inspecting objective to a mixture of the ion-exchange resin and a buffer liquid with specific pH and ion density.
CONSTITUTION: To a mixed liquid of ion-exchange resin and a phosphoric-acid buffer liquid of pH6.5W6.9 and ion density 0.01W0.1M, hemolysis inspecting objective added over a stir. Next, the above-mentioned buffer liquid is separated and the absorbance of the obtained buffer liquid is measured to obtain hemoglobin glucoside in blood. It is recommended to use 3W10ml of the phosphric-acid buffer liquid for 10W50μl of hemolysis inspecting objective. As the ion-exchange resin, weak acid positive ion exchange resin is used and an adequate container is filled with 1W2ml of resin. The absorbance of the buffer liquid after separation is measured in an ordinary 410nm photoelectric colorimetric method, thereby obtaining the quantity of hemoglobin glucoside.
COPYRIGHT: (C)1980,JPO&Japio
JP707379A 1979-01-26 1979-01-26 Simple measuring method for hemoglobin glucoside in blood Pending JPS55101052A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP707379A JPS55101052A (en) 1979-01-26 1979-01-26 Simple measuring method for hemoglobin glucoside in blood

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP707379A JPS55101052A (en) 1979-01-26 1979-01-26 Simple measuring method for hemoglobin glucoside in blood

Publications (1)

Publication Number Publication Date
JPS55101052A true JPS55101052A (en) 1980-08-01

Family

ID=11655895

Family Applications (1)

Application Number Title Priority Date Filing Date
JP707379A Pending JPS55101052A (en) 1979-01-26 1979-01-26 Simple measuring method for hemoglobin glucoside in blood

Country Status (1)

Country Link
JP (1) JPS55101052A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5872055A (en) * 1981-10-26 1983-04-28 Sogo Seibutsu Igaku Kenkyusho:Kk Simple method for measuring blood hemoglobin A↓1
US4407961A (en) * 1981-03-16 1983-10-04 Sanders James L Ion-exchange system and method for isolation and determination of glycosylated hemoglobin in human blood
WO1983003476A1 (en) * 1982-03-25 1983-10-13 James Lynn Sanders Ion-exchange system and method for isolation and determination of glycosylated hemoglobin in human blood

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5166894A (en) * 1974-09-09 1976-06-09 Denki Kagaku Keiki Kk Taikichu no tankasuisonodosokuteihoshiki

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5166894A (en) * 1974-09-09 1976-06-09 Denki Kagaku Keiki Kk Taikichu no tankasuisonodosokuteihoshiki

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4407961A (en) * 1981-03-16 1983-10-04 Sanders James L Ion-exchange system and method for isolation and determination of glycosylated hemoglobin in human blood
JPS5872055A (en) * 1981-10-26 1983-04-28 Sogo Seibutsu Igaku Kenkyusho:Kk Simple method for measuring blood hemoglobin A↓1
WO1983003476A1 (en) * 1982-03-25 1983-10-13 James Lynn Sanders Ion-exchange system and method for isolation and determination of glycosylated hemoglobin in human blood

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