JPS5644851A - Measurement of glycocylhemoglobin - Google Patents
Measurement of glycocylhemoglobinInfo
- Publication number
- JPS5644851A JPS5644851A JP12179779A JP12179779A JPS5644851A JP S5644851 A JPS5644851 A JP S5644851A JP 12179779 A JP12179779 A JP 12179779A JP 12179779 A JP12179779 A JP 12179779A JP S5644851 A JPS5644851 A JP S5644851A
- Authority
- JP
- Japan
- Prior art keywords
- azidomethohemoglobin
- glycocylhemoglobin
- azidomethoglycocylhemoglobin
- effuse
- spectrophotometer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005259 measurement Methods 0.000 title abstract 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract 1
- 102000001554 Hemoglobins Human genes 0.000 abstract 1
- 108010054147 Hemoglobins Proteins 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000003729 cation exchange resin Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 210000003743 erythrocyte Anatomy 0.000 abstract 1
- 239000000945 filler Substances 0.000 abstract 1
- 230000002949 hemolytic effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To measure the concentration with high precision using a spectrophotometer by separating and making azidomethoglycocylhemoglobin and azidomethohemoglobin effuse when glycocylhemoglobin is measured by chromatographic method.
CONSTITUTION: Specimens containing azidomethoglycocylhemoglobin and azidomethohemoglobin obtained by methoxylating with sodium azide, glycocylhemoglobin and hemoglobin contained in a hemolytic solution of erythrocytes, are obtained. Next, these specimens are supplied to a column containing weak acidic cation exchange resin as a filler or a liquid chromatographic device within a temperature range of 25°CW45°C (selective measurement temperature to be maintained within precison range of ±1°C). Then azidomethohemoglobin is caused to effuse with a developer (buffersolution) containing sodium axide. Thus it is possible to measure the concentration with high precision using the spectrophotometer.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12179779A JPS5644851A (en) | 1979-09-21 | 1979-09-21 | Measurement of glycocylhemoglobin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12179779A JPS5644851A (en) | 1979-09-21 | 1979-09-21 | Measurement of glycocylhemoglobin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5644851A true JPS5644851A (en) | 1981-04-24 |
Family
ID=14820146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12179779A Pending JPS5644851A (en) | 1979-09-21 | 1979-09-21 | Measurement of glycocylhemoglobin |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5644851A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011125484A1 (en) * | 2010-03-31 | 2011-10-13 | 積水メディカル株式会社 | Method of analyzing hemoglobins |
| JP2015165827A (en) * | 2010-04-09 | 2015-09-24 | 東洋紡株式会社 | Method of measuring glycated hemoglobin |
-
1979
- 1979-09-21 JP JP12179779A patent/JPS5644851A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011125484A1 (en) * | 2010-03-31 | 2011-10-13 | 積水メディカル株式会社 | Method of analyzing hemoglobins |
| CN102893147A (en) * | 2010-03-31 | 2013-01-23 | 积水医疗株式会社 | Method of analyzing hemoglobins |
| JPWO2011125484A1 (en) * | 2010-03-31 | 2013-07-08 | 積水メディカル株式会社 | Analysis method of hemoglobins |
| US9304139B2 (en) | 2010-03-31 | 2016-04-05 | Sekisui Medical Co., Ltd. | Method of analyzing hemoglobins |
| JP2015165827A (en) * | 2010-04-09 | 2015-09-24 | 東洋紡株式会社 | Method of measuring glycated hemoglobin |
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